Abstract：
:Fresh produce is often a source of enterohaemorrhagic Escherichia coli (EHEC) outbreaks. Fimbriae are extracellular structures involved in cell-to-cell attachment and surface colonisation. F9 (Fml) fimbriae have been shown to be expressed at temperatures lower than 37 °C, implying a function beyond the mammalian host. We demonstrate that F9 fimbriae recognize plant cell wall hemicellulose, specifically galactosylated side chains of xyloglucan, using glycan arrays. E. coli expressing F9 fimbriae had a positive advantage for adherence to spinach hemicellulose extract and tissues, which have galactosylated oligosaccharides as recognized by LM24 and LM25 antibodies. As fimbriae are multimeric structures with a molecular pattern, we investigated whether F9 fimbriae could induce a transcriptional response in model plant Arabidopsis thaliana, compared with flagella and another fimbrial type, E. coli common pilus (ECP), using DNA microarrays. F9 induced the differential expression of 435 genes, including genes involved in the plant defence response. The expression of F9 at environmentally relevant temperatures and its recognition of plant xyloglucan adds to the suite of adhesins EHEC has available to exploit the plant niche.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Holmes A,Rossez Y,Wright KM,Hedley PE,Morris J,Willats WGT,Holden NJdoi
10.3390/ijms21249720subject
Has Abstractpub_date
2020-12-19 00:00:00issue
24issn
1422-0067pii
ijms21249720journal_volume
21pub_type
杂志文章abstract：:The FLOWERING LOCUS T (FT) gene plays crucial roles in regulating the transition from the vegetative to reproductive phase. To understand the molecular mechanism of reproduction, three homologous FT genes were isolated and characterized from Cymbidium sinense "Qi Jian Bai Mo", Cymbidium goeringii and Cymbidium ensifol...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms130911385
更新日期：2012-01-01 00:00:00
abstract：:Alpha thalassaemia is highly prevalent in the plural society of Malaysia and is a public health problem. Haematological and molecular data from 5016 unrelated patients referred from various hospitals to the Institute for Medical Research for α thalassaemia screening from 2007 to 2010 were retrieved. The aims of this r...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms140918599
更新日期：2013-09-10 00:00:00
abstract：:Cholangiocarcinoma (CCA) is the second most common type of liver cancer, and is highly aggressive with very poor prognosis. CCA is classified into intrahepatic cholangiocarcinoma (iCCA) and extra-hepatic cholangiocarcinoma (eCCA), which is further stratified into perihilar (pCCA) and distal (dCCA). Cancer stem cells (...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20174154
更新日期：2019-08-25 00:00:00
abstract：:Mechanistic approaches to modeling the effects of ionizing radiation on cells are on the rise, promising a better understanding of predictions and higher flexibility concerning conditions to be accounted for. In this work we modified and extended a previously published mechanistic model of cell survival after photon i...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20236054
更新日期：2019-11-30 00:00:00
abstract：:The discovery of cell-free fetal DNA molecules in plasma of pregnant women has created a paradigm shift in noninvasive prenatal testing (NIPT). Circulating cell-free DNA in maternal plasma has been increasingly recognized as an important proxy to detect fetal abnormalities in a noninvasive manner. A variety of approac...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms18020453
更新日期：2017-02-20 00:00:00
abstract：:Somatic embryos (SE) have potential to rapidly form a whole plant. Generally, SE is thought to be derived from embryogenic calli (EC). However, in maize, not only embryogenic calli (EC, can generate SE) but also nonembryogenic calli (NEC, can't generate SE) can be induced from immature embryos. In order to understand ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19124004
更新日期：2018-12-12 00:00:00
abstract：:Alzheimer's disease (AD) is one of the most frequently diagnosed types of dementia in the elderly. An important pathological feature in AD is the aggregation and deposition of the β-amyloid (Aβ) in extracellular plaques. Transthyretin (TTR) can cleave Aβ, resulting in the formation of short peptides with less activity...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21239003
更新日期：2020-11-26 00:00:00
abstract：:Copper is an essential microelement that plays an important role in a wide variety of biological processes. Copper concentration has to be finely regulated, as any imbalance in its homeostasis can induce abnormalities. In particular, excess copper plays an important role in the etiopathogenesis of the genetic disease ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21031069
更新日期：2020-02-06 00:00:00
abstract：:Extracellular acidification occurs under physiologic and pathologic conditions, such as exercise, ischemia, and inflammation. It has been shown that acidosis has various adverse effects on bone. In recent years there has been increasing evidence which indicates that ovarian cancer G protein-coupled receptor 1 (OGR1) i...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms151222365
更新日期：2014-12-03 00:00:00
abstract：:Updated measurements of charged particle fluxes during the transit from Earth to Mars as well as on site measurements by Curiosity of Martian surface radiation fluxes identified potential health hazards associated with radiation exposure for human space missions. Designing mitigation strategies of radiation risks to a...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20010176
更新日期：2019-01-05 00:00:00
abstract：:Nut-based milks and yogurts are gaining popularity, but may not offer the same benefits as dairy yogurts to consumers. Cashew nuts often cause severe allergic reactions, and cashew nut allergens are stable to several types of processing. To compare its characteristics to dairy yogurt and characterize the effects of fe...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21218267
更新日期：2020-11-04 00:00:00
abstract：:It has been well confirmed ox-LDL plays key roles in the development of atherosclerosis via binding to LOX-1 and inducing apoptosis in vascular endothelial cells. Recent studies have shown ox-LDL can suppress microRNA has-let-7g, which in turn inhibits the ox-LDL induced apoptosis. However, details need to be uncovere...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms141122708
更新日期：2013-11-18 00:00:00
abstract：:Mathematical models are key to systems biology where they typically describe the topology and dynamics of biological networks, listing biochemical entities and their relationships with one another. Some (hyper)thermophilic Archaea contain an enzyme, called non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase (...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18040876
更新日期：2017-04-20 00:00:00
abstract：:The composition of IL-23R complex is similar to that of the IL-12 receptor (IL-12R) complex with a shared IL-12R-β1 chain. The IL-12R-β1 heterodimerizes with IL-23R and IL-12R-β2 to form IL-23R and IL-12R complexes, respectively. The IL-12R-β2 has been shown to function as a tumor suppressor gene and apoptotic inducer...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms141224656
更新日期：2013-12-18 00:00:00
abstract：:Chemerin, a novel adipokine, has been associated with metabolic, inflammatory, and atherosclerotic diseases. We aimed to determine the genetic basis of chemerin levels by conducting a genome-wide association study (GWAS) and to investigate the role of RARRES2 polymorphisms and circulating chemerin levels in the long-t...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20051174
更新日期：2019-03-07 00:00:00
abstract：:Genome instability is an essential hallmark in tumor development, including colorectal cancer. We have recently identified the oxysterol binding protein-related protein 3 (ORP3), also known as oxysterol binding protein-like 3 (OSBPL3), as a novel ploidy-control gene, whose knock-out leads to aneuploidy induction and p...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21165894
更新日期：2020-08-16 00:00:00
abstract：:Due to a typesetting error during layout, several references were incorrectly listed in [...]. ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,已发布勘误
doi：10.3390/ijms20112651
更新日期：2019-05-29 00:00:00
abstract：:Brain-derived neurotrophic factor (BDNF), a critical member of the neurotrophic family, plays an important role in multiple stress-related mental disorders. Although alterations in BDNF in multiple brain regions of individuals experiencing stress have been demonstrated in previous studies, it appears that a set of ele...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21041375
更新日期：2020-02-18 00:00:00
abstract：:Sequence-defined recombinant antibodies (rAbs) have emerged as alternatives to hybridoma-secreted monoclonal antibodies (mAbs) for performing immunoassays. However, the polyploidy nature of hybridomas often leads to the coexistence of aberrant or non-specific functional variable region (VR) gene transcripts, which com...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21186857
更新日期：2020-09-18 00:00:00
abstract：:KCND3 encodes the voltage-gated potassium ion channel subfamily D member 3, a six trans-membrane protein (Kv4.3), involved in the transient outward K+ current. KCND3 defect causes both cardiological and neurological syndromes. From a neurological perspective, Kv4.3 defect has been associated to SCA type 19/22, a compl...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21165802
更新日期：2020-08-13 00:00:00
abstract：:Autophagy primarily works to counteract nutrient deprivation that is strongly engaged during starvation and hypoxia, which happens in hypoperfusion. Nonetheless, autophagy is slightly active even in baseline conditions, when it is useful to remove aged proteins and organelles. This is critical when the mitochondria an...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms19092756
更新日期：2018-09-13 00:00:00
abstract：:Natural autoantibodies to the IGF1 receptor (IGF1R-aAb) have been described in relation to Graves' ophthalmopathy. Other physiological roles of natural IGF1R-aAb are not known. We hypothesized that IGF1R-aAb may be related to muscle development. Serum samples (n = 408) from young overweight subjects (n = 143) were col...
journal_title：International journal of molecular sciences
pub_type： 临床试验,杂志文章
doi：10.3390/ijms21020463
更新日期：2020-01-11 00:00:00
abstract：:Cell therapy has been evaluated to enhance heart function after injury. Delivered cells mostly act via paracrine mechanisms, including secreted growth factors, cytokines, and vesicles, such as exosomes (Exo). Intramyocardial injection of cardiac-resident progenitor cells (CPC)-derived Exo reduced scarring and improved...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20030468
更新日期：2019-01-22 00:00:00
abstract：:Aqueous solutions of ethoxylated alcohols which form lyotropic liquid crystals at high concentrations (40-80%) were selected as model lubricating substances. Microscopic studies under polarized light and viscosity measurements were carried out in order to confirm the presence of liquid crystalline structures in the ca...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms11010189
更新日期：2010-01-12 00:00:00
abstract：:This work assesses the effect of chemical induction with isopropyl β-D-1-thiogalactopyranoside (IPTG) on the expression of enhanced green fluorescent protein (eGFP) by planktonic and biofilm cells of Escherichia coli JM109(DE3) transformed with a plasmid containing a T7 promoter. It was shown that induction negatively...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21020576
更新日期：2020-01-16 00:00:00
abstract：:Ischemic preconditioning (IPre) reduces ischemia/reperfusion (I/R) injury in the heart. The non-coding microRNA miR-125b-1-3p has been demonstrated to play a role in the mechanism of IPre. Hypercholesterolemia is known to attenuate the cardioprotective effect of preconditioning; nevertheless, the exact underlying mech...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21113744
更新日期：2020-05-26 00:00:00
abstract：:In patients with glioblastoma, antiangiogenic therapy with bevacizumab (BEV) has been shown to improve progression-free survival (PFS), but not overall survival (OS). Especially in patients with an unusual infiltrative phenotype as seen in multifocal glioblastoma, the use of BEV therapy is still more controversial. Th...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18112469
更新日期：2017-11-20 00:00:00
abstract：:Osteoporosis represents the most common bone disease worldwide and results in a significantly increased fracture risk. Extrinsic and intrinsic factors implicated in the development of osteoporosis are also associated with delayed fracture healing and impaired bone regeneration. Based on a steadily increasing life expe...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20010083
更新日期：2018-12-26 00:00:00
abstract：:It is well known that cobalt chloride (CoCl2) can enhance the stability of hypoxia-inducible factor (HIF)-1α. The aim of this study is to detect the effect of CoCl2 on the hypoxia tolerance of mice which were repeatedly exposed to autoprogressive hypoxia. Balb/c mice were randomly divided into groups of chemical pretr...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms150610999
更新日期：2014-06-18 00:00:00
abstract：:Sarcopenia has a significant negative impact on healthspan in the elderly and effective pharmacologic interventions remain elusive. We have previously demonstrated that sarcopenia is associated with reduced activity of the sarcoplasmic reticulum Ca2+ ATPase (SERCA) pump. We asked whether restoring SERCA activity using...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms22010037
更新日期：2020-12-22 00:00:00
