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The complement-mediated prozone effect in the Luminex single-antigen bead assay and its impact on HLA antibody determination in patient sera.

Abstract：

:The Luminex xMAP system has become an important tool for HLA antibody screening and identification in sera of transplant patients. Recently, the Luminex single antigen bead assay was shown to be prone to an artefact, the so called prozone phenomenon: Sera with high titer HLA antibodies gave negative results when tested neat, but reacted strongly positive after 1:10 dilution. We also observed such a phenomenon and found that it was most likely caused by the complement component 1 (C1) by competitively displacing the detection antibodies. In this article we review the complement-mediated prozone effect and other mechanisms of interference with solid phase assays, and we discuss possible consequences for HLA antibody testing with the Luminex SAB assay.

journal_name

Int J Immunogenet

journal_title

International journal of immunogenetics

authors

Weinstock C,Schnaidt M

doi

10.1111/j.1744-313X.2012.01147.x

subject

Has Abstract

pub_date

2013-06-01 00:00:00

pages

171-7

issue

3

eissn

1744-3121

issn

1744-313X

journal_volume

40

pub_type

杂志文章,评审

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