Abstract:
:In this study, we aimed to find new genes associated with rheumatoid arthritis (RA) so that more comprehensive genes would be used for monitoring and/or diagnosing patients. Illumina digital gene expression profiling was applied in two sample types - peripheral blood mononuclear cells (PBMCs) and synovial cells to compare the gene expression pattern between 17 patients with RA and three control groups (six osteoarthritis patients, three ankylosing spondylitis patients and 17 healthy controls). Bioinformatics was performed on pathway analysis and protein-protein interaction networks. Four novel genes from PBMCs - DHRS3, TTC38, SAP30BP and LPIN2 - were found to be associated with RA and further confirmed through quantitative real-time polymerase chain reaction. Five new differentially expressed genes (EPYC, LIFR, GLDN, TADA3 and ZNRF3) found in synovial cells were not confirmed. Pathway analyses revealed 10 significantly enriched pathways, and a protein-protein interaction network analysis showed that four novel PBMC-derived genes were connected to previously reported genes by four intermediate genes. Therefore, we proposed that four newly identified PBMC-derived genes could be integrated with previously reported RA-associated genes to monitor and/or diagnose RA.
journal_name
Int J Immunogenetjournal_title
International journal of immunogeneticsauthors
Wang H,Guo J,Jiang J,Wu W,Chang X,Zhou H,Li Z,Zhao Jdoi
10.1111/iji.12313subject
Has Abstractpub_date
2017-06-01 00:00:00pages
107-113issue
3eissn
1744-3121issn
1744-313Xjournal_volume
44pub_type
杂志文章abstract::Leprosy is one of the most neglected infectious tropical diseases of the skin and the nerves caused by the intracellular pathogen Mycobacterium leprae. The inducible NOS isoform encoded by NOS2A plays a vital role in host defence against bacterial infections. The functional promoter polymorphisms in NOS2A are associat...
journal_title:International journal of immunogenetics
pub_type: 杂志文章
doi:10.1111/iji.12108
更新日期:2014-06-01 00:00:00
abstract::Immunological inflammatory reaction is one of the key links in the occurrence and development of post-traumatic osteomyelitis after microbial invasion. Growing evidence suggests complex interactions between IFN-γ and bone remodelling cells. However, potential association of IFN-γ gene polymorphism with susceptibility ...
journal_title:International journal of immunogenetics
pub_type: 杂志文章
doi:10.1111/iji.12462
更新日期:2020-04-01 00:00:00
abstract::Pemphigus foliaceus (PF) is an autoimmune bullous epidermal disease, characterized by autoantibodies specific to the desmosomal protein desmoglein 1 (dsg1) and by acantholysis, the rupture of the cellular junctions among keratinocytes. Known also as fogo selvagem (wild fire) in Brazil, the disease has distinct epidemi...
journal_title:International journal of immunogenetics
pub_type: 杂志文章
doi:10.1111/j.1744-313X.2006.00585.x
更新日期:2006-04-01 00:00:00
abstract::Autoimmune diabetes [type 1 diabetes mellitus (T1DM), latent autoimmune diabetes in adults (LADA) and part of malnutrition-related diabetes] has been shown to have genetic predisposition. Studies in IDDM 5 have lead to the discovery of a novel polymorphism 163 A-->G, of SUMO4 (small ubiquitin-related modifier) gene, a...
journal_title:International journal of immunogenetics
pub_type: 杂志文章
doi:10.1111/j.1744-313X.2007.00668.x
更新日期:2007-04-01 00:00:00
abstract::Single nucleotide polymorphisms (SNPs) in immune-related genes have been shown to play a role in driving the development of the severe phenotypes of dengue virus (DENV) infection. We assessed the association between IFNL3 gene SNP (rs12979860) and dengue clinical outcomes in children. Patients with dengue-related symp...
journal_title:International journal of immunogenetics
pub_type: 杂志文章
doi:10.1111/iji.12477
更新日期:2020-08-01 00:00:00
abstract::In the present study, DNA typing for human leucocyte antigen (HLA)-DPB1, -DRB1, and -DQB1 was performed using polymerase chain reaction-sequence-based-typing (PCR-SBT) method on 94 randomly selected, healthy, unrelated individuals from the Ewenki ethnic population in Inner Mongolia Autonomous Region of China. A total ...
journal_title:International journal of immunogenetics
pub_type: 杂志文章
doi:10.1111/j.1744-313X.2007.00718.x
更新日期:2007-12-01 00:00:00
abstract::This study assessed the phenotypic and allelic profiles of ABO and Rhesus D blood group system among first time blood donors at the National Centre of Blood Supply of Antananarivo. We collected through this retrospective study all data registered during 7 years of practice (from 2003 to 2009). Age and sex were analyse...
journal_title:International journal of immunogenetics
pub_type: 杂志文章
doi:10.1111/j.1744-313X.2012.01120.x
更新日期:2012-12-01 00:00:00
abstract::Genes involved in regulation of the nuclear factor - kappa B (NF-κB) pathway are suggested to play a role in the pathogenesis of acute myeloid leukaemia (AML). The present study aimed to assess the association between the NF-κB1, TRAF3 and TLRs genes single nucleotide polymorphisms (SNPs) and disease susceptibility as...
journal_title:International journal of immunogenetics
pub_type: 杂志文章
doi:10.1111/iji.12255
更新日期:2016-04-01 00:00:00
abstract::Perforin is one of the key effector molecules of cytotoxic T cells and natural killer cells. The influence of HLA-DRB1 alleles on peripheral blood perforin-positive CD4, CD8, CD16 and CD 56 cells was studied by flow cytometry. HLA-DRB1 typing was done in normal healthy subjects (NHS: n = 156) and patients with pulmona...
journal_title:International journal of immunogenetics
pub_type: 杂志文章
doi:10.1111/j.1744-313X.2007.00703.x
更新日期:2007-10-01 00:00:00
abstract::Matrix metalloproteinase 9 plays an important role in the development of bronchial asthma. We were interested in whether the polymorphisms -T1702A, -C1562T, R279Q and +C6T within the matrix metalloproteinase 9 (MMP-9) gene were associated with asthma in a population of 231 asthmatic children. However, we found no asso...
journal_title:International journal of immunogenetics
pub_type: 杂志文章
doi:10.1111/j.1744-313X.2005.00516.x
更新日期:2005-08-01 00:00:00
abstract::Tumour necrosis factor-alpha (TNFalpha) has been implicated in the pathogenicity of severe sepsis by both genetic association studies and animal models. Conflicting functional data have emerged in relation to genetic variants and TNFalpha protein production. Therefore, we assessed the functionality of TNFalpha genetic...
journal_title:International journal of immunogenetics
pub_type: 杂志文章
doi:10.1111/j.1744-313X.2008.00773.x
更新日期:2008-08-01 00:00:00
abstract::Single nucleotide polymorphisms within the promoter or other regulatory sequences of cytokine genes were evaluated and compared between an Iranian population and populations of different ethnic/geographical background. In 40 healthy Iranian subjects, cytokine typing was performed by polymerase chain reaction-sequence-...
journal_title:International journal of immunogenetics
pub_type: 杂志文章,收录出版
doi:10.1111/j.1744-313X.2006.00595.x
更新日期:2006-06-01 00:00:00
abstract::Haematopoietic stem cell transplantation (HSCT), using unrelated donors (UD), is now a common modality of treatment for individuals with a variety of different diseases. HLA matching has been shown to have a significant impact on patient outcome. This study includes 423 unrelated UK patient/donor pairs. The patients a...
journal_title:International journal of immunogenetics
pub_type: 杂志文章
doi:10.1111/j.1744-313X.2008.00793.x
更新日期:2008-08-01 00:00:00
abstract::Insulin resistance (IR) and pancreatic beta-cell dysfunction are usual comorbidities in polycystic ovary syndrome (PCOS). Vascular endothelial growth factor (VEGF) is known to play an important role in the pathogenesis of PCOS. This study examined firstly the possible association of common +405 G/C,-460 T/C and -2578 ...
journal_title:International journal of immunogenetics
pub_type: 杂志文章
doi:10.1111/j.1744-313X.2010.00915.x
更新日期:2010-08-01 00:00:00
abstract::The single nucleotide polymorphism (SNP) G949T in the mannose-binding lectin ( MBL ) 1 gene has been associated with low MBL-A concentration in serum and detected at different frequencies in various European pig populations. However, the origin of this SNP is not known. Part of the MBL1 gene was sequenced in 12 wild b...
journal_title:International journal of immunogenetics
pub_type: 杂志文章
doi:10.1111/j.1744-313X.2012.01132.x
更新日期:2013-04-01 00:00:00
abstract::We examined five single nucleotide polymorphisms (SNPs) and reconstructed 5-locus haplotypes of the CCL2 gene, in knee osteoarthritis (OA) cases and in controls. The CCL2 rs2857657 variant (G) allele was observed more frequently in female knee OA cases than in controls. One haplotype (H5) was observed exclusively in t...
journal_title:International journal of immunogenetics
pub_type: 杂志文章
doi:10.1111/iji.12015
更新日期:2013-08-01 00:00:00
abstract::We report the identification of a novel HLA-DRB1*04, officially named DRB1*0460. It was detected during performing HLA-DRB1 high resolution typing by DNA sequencing-based method. The exon 2 nucleotide sequence of DRB1*0460 is identical to that of DRB1*040301 except at codon 63 (AGC-->AAC), changing the encoded serine ...
journal_title:International journal of immunogenetics
pub_type: 杂志文章
doi:10.1111/j.1744-313X.2006.00641.x
更新日期:2007-02-01 00:00:00
abstract::Illumina first introduced their TruSight human leucocyte antigen (HLA) next-generation sequencing (NGS) typing kit in 2015 and subsequently followed up with a new version in 2016. Here we report on our experience comparing the two versions of the Illumina HLA NGS kits. ...
journal_title:International journal of immunogenetics
pub_type: 杂志文章
doi:10.1111/iji.12322
更新日期:2017-08-01 00:00:00
abstract::Interleukin-18 (IL-18) plays a vital role in both innate and acquired immunity. We analysed polymorphisms at -607(C/A) and -137(G/A) in the promoter region of IL-18 gene by allele-specific polymerase chain reaction in normal healthy subjects (n = 173) and patients with pulmonary tuberculosis (n = 165). Allele, genotyp...
journal_title:International journal of immunogenetics
pub_type: 杂志文章
doi:10.1111/j.1744-313X.2007.00714.x
更新日期:2007-10-01 00:00:00
abstract::We propose a bioinformatics pipeline in which we use an ESTs database to predict and validate single-nucleotide polymorphisms (SNPs) directly linked to gene-coding regions at the HLA class I genes (HLA-A, HLA-B and HLA-C). Annotation originated from our analysis revealed various classes of possible new variations that...
journal_title:International journal of immunogenetics
pub_type: 杂志文章
doi:10.1111/j.1744-313X.2011.01076.x
更新日期:2012-06-01 00:00:00
abstract::HLA-DQB1*06:48 has single nucleotide polymorphisms within codons 70 and 62 of exon 2 (GGG>AGG and AAG>AAC) relative to HLA-DQB1*06:02:01 and HLA-DQB1*06:37. This results in amino acid differences (G>R and K>N) that will change the polarity and charge of the encoded antigen and may therefore affect its peptide repertoi...
journal_title:International journal of immunogenetics
pub_type: 杂志文章
doi:10.1111/iji.12004
更新日期:2013-08-01 00:00:00
abstract::The distributions of HLA allele and haplotype are variable in different ethnic populations and the data for some populations have been published. However, the data on HLA-C and HLA-DQB1 loci and the haplotype of HLA-A, HLA-B, HLA-C, HLA-DRB1 and HLA-DQB1 loci at a high-resolution level are limited in Zhejiang Han popu...
journal_title:International journal of immunogenetics
pub_type: 杂志文章
doi:10.1111/iji.12411
更新日期:2019-02-01 00:00:00
abstract::The present study aimed to analyse the frequencies of human leukocyte antigen HLA-ABCDQB1 and HLA-DRB1 alleles and haplotypes in a subset of 3,732 Han population from Hubei of China. All samples were typed in the HLA-ABCDQB1 and HLA-DRB1 loci using the sequence-based typing method; subsequently, the HLA polymorphisms ...
journal_title:International journal of immunogenetics
pub_type: 杂志文章
doi:10.1111/iji.12516
更新日期:2021-02-01 00:00:00
abstract::The Luminex xMAP system has become an important tool for HLA antibody screening and identification in sera of transplant patients. Recently, the Luminex single antigen bead assay was shown to be prone to an artefact, the so called prozone phenomenon: Sera with high titer HLA antibodies gave negative results when teste...
journal_title:International journal of immunogenetics
pub_type: 杂志文章,评审
doi:10.1111/j.1744-313X.2012.01147.x
更新日期:2013-06-01 00:00:00
abstract::HLA donor-specific antibodies developed de novo after transplant remain a major cause of chronic allograft dysfunction. Our study main purpose was to determine whether HLA MM, assessed traditionally and by HLA total and AbVer eplet mismatch load (EptMM and EpvMM) assessed with HLAMatchMaker, had impact on dnDSA develo...
journal_title:International journal of immunogenetics
pub_type: 杂志文章
doi:10.1111/iji.12519
更新日期:2021-02-01 00:00:00
abstract::A novel MICB allele, MICB*030, has been identified in a healthy Chinese individual of Mongol ethnicity residing in northern China by polymerase chain reaction sequence-based typing (PCR-SBT) and confirmed by cloning and sequencing. MICB*030 was linked to HLA-B*35. Aligned with MICB*005:02, MICB*030 has a nonsynonymous...
journal_title:International journal of immunogenetics
pub_type: 杂志文章
doi:10.1111/iji.12207
更新日期:2015-08-01 00:00:00
abstract::Induced heteroduplex genotyping (IHG) is one of many methods that can be used to determine single nucleotide polymorphisms (SNPs). It is relatively new in comparison to other polymerase chain reaction (PCR)-based techniques. The aim of this study was to compare the results of genotyping using IHG with the results of g...
journal_title:International journal of immunogenetics
pub_type: 杂志文章
doi:10.1111/j.1744-313X.2005.00494.x
更新日期:2005-04-01 00:00:00
abstract::According to the IMGT/HLA Database, the DNA sequence of A*11:53 is identical to A*11:02:01 in exons 2, 3, 4 and 5 except at codon 276. A*11:53 was reported as a rare variant of A*11, while A*11:02:01 was understood to be the second most frequently observed variant of A*11 after A*11:01:01 in Taiwanese. We sequenced HL...
journal_title:International journal of immunogenetics
pub_type: 杂志文章
doi:10.1111/j.1744-313X.2012.01121.x
更新日期:2012-12-01 00:00:00
abstract::In recent years, with the application of genotyping technology, there has been a substantial increase in the number of reported blood group alleles. This survey was designed to evaluate new molecular blood group genotyping methods and compile reference blood group data sets for Polynesian and Maori subjects. Subsequen...
journal_title:International journal of immunogenetics
pub_type: 杂志文章
doi:10.1111/iji.12073
更新日期:2013-12-01 00:00:00
abstract::Knowledge on blood group phenotypes is of key importance in clinical practice. It used in blood transfusion practice to determine the direction of recruitment of voluntary donors as required for each population within a country, and for disease association and population genetics studies. This study aimed at reporting...
journal_title:International journal of immunogenetics
pub_type: 杂志文章
doi:10.1111/iji.12412
更新日期:2019-04-01 00:00:00