Abstract：
:A amphiphilic block copolymer composed of conventional monomethoxy (polyethylene glycol)-poly (d,l-lactide-co-glycolide)-poly (l-lysine) (mPEG-PLGA-b-PLL) was synthesized. The chemical structure of this copolymer and its precursors was confirmed by Fourier Transform Infrared Spectroscopy (FTIR), (1)H Nuclear Magnetic Resonance ((1)H NMR) and Gel Permeation Chromatography (GPC). The copolymer was used to prepare nanoparticles (NPs) that were then loaded with either the anti-cancer drug adriamycin or small interfering RNA-negative (siRNA) using a double emulsion method. MTT assays used to study the in vitro cytotoxicity of mPEG-PLGA-b-PLL NPs showed that these particles were not toxic in huh-7 hepatic carcinoma cells. Confocal laser scanning microscopy (CLSM) and flow cytometer analysis results demonstrated efficient mPEG-PLGA-b-PLL NPs-mediated delivery of both adriamycin and siRNA into the cells. In vivo the targeting delivery of adriamycin or siRNA mediated by mPEG-PLGA-b-PLL NPs in the huh-7 hepatic carcinoma-bearing mice was evaluated using a fluorescence imaging system. The targeting delivery results and froze section analysis confirmed that drug or siRNA is deliver to tumor more efficiently by mPEG-PLGA-b-PLL NPs than free drug or Lipofectamine™2000. The high efficiency delivery of mPEG-PLGA-b-PLL NPs mainly due to the enhancement of cellular uptake. These results imply that mPEG-PLGA-b-PLL NPs have a great potential to be used as an effective carriers for adriamycin or siRNA.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Liu P,Yu H,Sun Y,Zhu M,Duan Ydoi
10.1016/j.biomaterials.2012.02.041subject
Has Abstractpub_date
2012-06-01 00:00:00pages
4403-12issue
17eissn
0142-9612issn
1878-5905pii
S0142-9612(12)00246-3journal_volume
33pub_type
杂志文章相关文献
BIOMATERIALS文献大全abstract：:Titiania nanotubes have large potential in medical implant applications but their tissue compatibility is still controversial. Since the sterilization methods may impact the biocompatibility of titania nanotubes and be the source of the controversy, we investigate the influence of three commonly used sterilization met...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2009.11.103
更新日期：2010-03-01 00:00:00
abstract：:Lymphocytes typically interact with implanted biomaterials through adsorbed exogenous proteins. To provide a more complete characterization of these interactions, analysis of lymphocyte migration on adsorbed extracellular matrix proteins must accompany the commonly performed adhesion studies. We report here a comparis...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(99)00154-4
更新日期：1999-12-01 00:00:00
abstract：:Acute and chronic wounds affect millions and are associated with billions of dollars in healthcare costs. The use of healing markers, biochemical cues from biocompatible matrices and materials, and their correlation with wound healing has the potential to generate valuable diagnostic, prognostic, and therapeutic infor...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2019.05.007
更新日期：2019-08-01 00:00:00
abstract：:The design of bioartificial liver assist device requires an effective attachment of primary hepatocytes on polymeric biomaterials. A better understanding of this cell-surface interaction would aid the optimal choice of biomaterials. In this study, the adhesion contact dynamics of primary hepatocytes on poly(ethylene t...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2004.03.041
更新日期：2005-03-01 00:00:00
abstract：:Silicon (Si) substitution in the crystal structures of calcium phosphate (CaP) ceramics such as hydroxyapatite (HA) and tricalcium phosphate (TCP) generates materials with superior biological performance to stoichiometric counterparts. Si, an essential trace element required for healthy bone and connective tissues, in...
journal_title：Biomaterials
pub_type： 杂志文章,评审
doi：10.1016/j.biomaterials.2007.05.003
更新日期：2007-10-01 00:00:00
abstract：:mRNA-based vaccines have the benefit of triggering robust anti-cancer immunity without the potential danger of genome integration from DNA vaccines or the limitation of antigen selection from peptide vaccines. Yet, a conventional mRNA vaccine comprising of condensed mRNA molecules in a positively charged protein core ...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2017.02.019
更新日期：2017-05-01 00:00:00
abstract：:Photolithography is the current workhorse for the microelectronic industry. It has been used extensively for the creation of patterns on two-dimensional surfaces. Various research groups have studied the use of photolithography to pattern surfaces for the alignment of cells. So far, these applications have been limite...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2003.08.055
更新日期：2004-05-01 00:00:00
abstract：:Tissue engineering strategies for repairing and regenerating articular cartilage face critical challenges to recapitulate the dynamic and complex biochemical microenvironment of native tissues. One approach to mimic the biochemical complexity of articular cartilage is through the use of recombinant bacterial collagens...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2016.05.011
更新日期：2016-08-01 00:00:00
abstract：:Employing equilibrium dialysis, the binding abilities of Cu, Al, Co and Cr ions from corroded Cu-Al and Co-Cr dental casting alloys towards human saliva and two of its gel chromatographic fractions were determined. Results indicate that both Cu and Co bind to human saliva i.e. 0.045 and 0.027 mg/mg protein, respective...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(85)90001-8
更新日期：1985-05-01 00:00:00
abstract：:Ocular surface diseases including conjunctival disorders are multifactorial progressive conditions that can severely affect vision and quality of life. In recent years, stem cell therapies based on conjunctival stem cells (CjSCs) have become a potential solution for treating ocular surface diseases. However, neither a...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2020.120462
更新日期：2021-01-01 00:00:00
abstract：:The metabolic disposition and elimination process of the anhydride co-polymer poly[1,3-bis(p-carboxy-phenoxypropane):sebacic acid] 20:80 [P(CPP:Sa)20:80] implanted in the rat brain was studied. Two polymers were prepared, one with [14C]SA and unlabelled CPP, and the other co-polymer with [14C]CPP and unlabelled SA. Wi...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(94)90166-x
更新日期：1994-07-01 00:00:00
abstract：:The aim of this study was to investigate the potential protective role of fullerenol C60(OH)24 on doxorubicin-induced liver toxicity using in vivo (female Sprague-Dawley rats) and in vitro (human hepatocellular carcinoma - HepG2; colorectal adenocarcinoma cell lines - Caco-2) approaches. The first (healthy control) an...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2008.04.048
更新日期：2008-08-01 00:00:00
abstract：:Bone tissue is an anisotropic non-homogeneous composite material composed of inorganic, bone mineral fibres (hydroxyapatite) embedded in an organic matrix (type I collagen and non-collagenous proteins). Factors contributing to the overall mechanical behaviour include constituent volume fraction, mechanical properties,...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(94)90263-1
更新日期：1994-01-01 00:00:00
abstract：:Mucus layer coating the vaginal epithelium represents a barrier for intravaginally delivered recombined adenoviral (rAd) vectors, but it could be overcome by proper polyethylene glycol (PEG) modification. Here we synthesized two cationic PEG derivatives, amino-(EO)n/(AGE)m-Cyss (APCs). The polymers contained neutral l...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2014.05.056
更新日期：2014-09-01 00:00:00
abstract：:Thin and defect-free calcium phosphate films with a Ca/P ratio of 1.62 were formed by electron-beam evaporation. The as-deposited films had average bonding strengths to the metal implants of 64.8 MPa and the dissolution rates of 47.5 nm/h in isotonic saline solutions. The interface mechanical characteristics and histo...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(01)00147-8
更新日期：2002-01-01 00:00:00
abstract：:The development of biohybrid organs (BHO) will benefit from improved membranes regarding transport and cell contacting properties. Here we describe in a first study the development and testing of membranes made of polyacrylonitrile (PAN) and polysulfone (PSU) for the immobilisation of kidney epithelial cells. Comparat...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(99)00147-7
更新日期：2000-01-01 00:00:00
abstract：:We investigated the morphological effect of phase-separated block copolymer surfaces composed of poly(2-methacryloyloxyethyl phosphorylcholine (MPC)) (PMPC) and poly(dimethylsiloxane) (PDMS) on protein adsorption and cell adhesion behavior. We observed three different types of phase-separated surface morphologies by T...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2009.06.031
更新日期：2009-10-01 00:00:00
abstract：:The concentrations of soluble copper and tin which were generated by the corrosion of a number of different amalgams in both an artificial saliva and Ringer's solution are reported. Besides variations in solution composition, the effects of solution agitation and abrasive particle wear upon the release of soluble spec...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(84)90015-2
更新日期：1984-07-01 00:00:00
abstract：:Regeneration of the damaged myocardium is one of the most challenging fronts in the field of tissue engineering due to the limited capacity of adult heart tissue to heal and to the mechanical and structural constraints of the cardiac tissue. In this study we demonstrate that an engineered acellular scaffold comprising...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2013.08.017
更新日期：2013-12-01 00:00:00
abstract：:The present study has examined the efficacy of a polymer microarray platform to screen a library of polyurethanes for applications such as human skeletal progenitor cell isolation and surface modification of tissue engineering scaffolds to enhance skeletal cell growth and differentiation. Analysis of polyurethane micr...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2008.10.038
更新日期：2009-02-01 00:00:00
abstract：:Methyl methacrylate (MMA) is a volatile liquid widely used in the manufacture of acrylic polymers. In modern dentistry, MMA is the mainstream material in denture bases. MMA has been implicated as primary irritant and sensitizer, which can cause allergic eczematous reaction on the oral mucosa as well as skin. To date, ...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(03)00132-7
更新日期：2003-08-01 00:00:00
abstract：:Endotoxemia is a severe pathophysiology induced by bacterial endotoxin (also known as lipopolysaccharide, LPS), causing high mortality in clinic due to the life-threatening syndromes, such as sepsis, shock, and multiple organ dysfunction. Removing or neutralizing endotoxin from the circulatory system has been proven t...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2018.10.029
更新日期：2019-01-01 00:00:00
abstract：:Cationic nanoparticles are a promising class of transfection agents for oligonucleotide and gene delivery, but vary greatly in their effectiveness and cytotoxicity. Recently, we developed a new class of cationic transfection agents based on cationic shell-crosslinked knedel-like nanoparticles (cSCKs) that efficiently ...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2009.10.033
更新日期：2010-03-01 00:00:00
abstract：:Cutaneous melanoma is an increasingly common and potentially lethal malignancy of melanocytes, the melanin producing cells normally located in the basal layer of the skin epidermis. Despite major advances in cancer chemotherapeutics and immunotherapy, the success in treating metastatic melanoma remains poor. The notio...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2011.08.056
更新日期：2011-12-01 00:00:00
abstract：:This study examined the engineering and true axial stress-strain behavior of smooth cylindrical and shallow and deep notched cylindrical test specimens, under applied axial tensile loading using non-contacting methods, of both conventional and highly crosslinked ultra-high molecular weight polyethylenes (UHMWPEs). The...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2004.09.031
更新日期：2005-06-01 00:00:00
abstract：:Programmable hydrogels are defined as hydrogels that are able to change their properties and functions periodically, reversibly and/or sequentially on demand. They are different from those responsive hydrogels whose changes are passive or cannot be stopped or reversed once started and vice versa. The purpose of this r...
journal_title：Biomaterials
pub_type： 杂志文章,评审
doi：10.1016/j.biomaterials.2018.03.008
更新日期：2018-09-01 00:00:00
abstract：:We investigated the bioactivity of new chitosan-based multi-membrane hydrogel (MMH) architectures towards chondrocyte-like cells. The microstructure of the hydrogels constituting the membranes precludes any living cell penetration, whereas their lower scale architecture allows the protein diffusion. The biological beh...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2011.04.012
更新日期：2011-08-01 00:00:00
abstract：:Porous calcium phosphate ceramics consisting of hydroxyapatite (HA) and tricalcium phosphate (TCP) with different HA to TCP ratio were implanted intramuscularly in rabbits for six months in order to carry out a comparative study on osteogenic activity of the ceramics. Bone formation was detected only in HT73 (HA to TC...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(01)00119-3
更新日期：2002-01-01 00:00:00
abstract：:Silicone elastomers have been used as implants in orthopaedics for replacements of small joints such as the metacarpophalangeal joint. A series of experiments was conducted an axially tensioned silicone elastomer tendon spacer samples to determine whether there is a relationship between stress in the material and imag...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(97)00155-5
更新日期：1998-01-01 00:00:00
abstract：:Effective real-time and long-time in vivo imaging for flap perfusion requires bright and stable imaging agents whose emissions can effectively penetrate live tissues without photobleaching. Compared to the standard imaging agent today - intraoperative indocyanine green (ICG), quantum dots (QDs) is a more attractive al...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2016.06.043
更新日期：2016-10-01 00:00:00
