Abstract：
OBJECTIVE:To characterize the BET gene expression in human testis with spermatogenetic impairments; to examine BRDT protein expression in testis and semen. DESIGN:Prospective study. SETTING:Fertility clinic. PATIENT(S):Azoospermic men (n = 120) who underwent testicular sperm extraction and who were classified as either normal spermatogenesis, mixed atrophy, spermatocyte maturation arrest, or Sertoli cells only according to their combined histologic and cytologic testicular findings and three normozoospermic men who donated sperm. INTERVENTION(S):Evaluation of testicular biopsies by qualitative and quantitative reverse transcriptase-polymerase chain reaction, immunohistochemical staining, and analysis of spermatozoa by immunofluorescence. MAIN OUTCOME MEASURE(S):Expression of the four BET genes in testis and localization of BRDT protein in testicular tissue and ejaculated spermatozoa. RESULT(S):The BRDT gene was not expressed in testicular tissue from patients with Sertoli cells only, whereas the other three genes of the BET family retained expression in all the pathologies. The BRDT protein was localized in the nuclei of spermatocytes, spermatids, and ejaculated spermatozoa. Expression of BRDT protein was almost nil in testicular tissue specimens with spermatocyte maturation arrest despite normal transcript levels. CONCLUSION(S):Human BRDT expression pattern differs from mouse BRDT expression. In human, BRDT is the only BET gene expressed exclusively in testicular germ cells. Its expression in elongated spermatids and ejaculated spermatozoa raises the possibility that it is involved in unidentified additional functions.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Barda S,Paz G,Yogev L,Yavetz H,Lehavi O,Hauser R,Botchan A,Breitbart H,Kleiman SEdoi
10.1016/j.fertnstert.2011.10.010subject
Has Abstractpub_date
2012-01-01 00:00:00pages
46-52.e5issue
1eissn
0015-0282issn
1556-5653pii
S0015-0282(11)02676-8journal_volume
97pub_type
杂志文章abstract：:Turner syndrome (TS), which affects approximately 1 in 2,500 live-born females, is characterized by loss or structural anomalies of an X chromosome. Clinical features vary among patients; multiple organ systems can be affected. Endocrinologists are involved in the management of short stature, delayed puberty, and infe...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/j.fertnstert.2012.07.1104
更新日期：2012-10-01 00:00:00
abstract：:One hundred seventy-seven women aged 41 +/- 8 (mean +/- SD) years, referred for evaluation of excessive uterine bleeding, were enrolled in an open-label randomized trial to evaluate the efficacy of local anesthesia before hysteroscopy in an outpatient population. The patients underwent hysteroscopy and endometrial bio...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章,随机对照试验
doi：
更新日期：1994-11-01 00:00:00
abstract：:Hemochromatosis is a rare disorder of iron storage. This report illustrates a case of hypogonadotropic-hypogonadism in a female with biopsy-proven hemochromatosis. Dynamic pituitary and gonadal testing revealed subnormal gonadotropin responses to gonadotropin-releasing hormone (GnRH) but normal ovarian reserve, as sho...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)53842-4
更新日期：1990-10-01 00:00:00
abstract：OBJECTIVE:To compare final embryo disposition between patients and donor oocyte recipients. DESIGN:Retrospective study. SETTING:Private infertility practice. PATIENT(S):Patients undergoing IVF with embryo cryopreservation. INTERVENTION(S):None. MAIN OUTCOME MEASURE(S):Final cryopreserved embryo disposition. RESUL...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2010.08.002
更新日期：2011-03-01 00:00:00
abstract：:Although salpingitis frequently produces tubal damage and infertility, many women with tubal factor as a cause of their infertility do not have a clinical history of salpingitis. In order to investigate whether or not some such cases might be due to subclinical chlamydial infections, we measured antichlamydial antibod...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)46634-3
更新日期：1982-11-01 00:00:00
abstract：OBJECTIVE:To determine the levels of metabolites surrounding the human oocyte and embryo in vivo. DESIGN:Oviduct and uterine fluids were collected throughout the menstrual cycle. Cumulus cells were collected at oocyte retrieval and their production of metabolites was assessed. Samples were analyzed for pyruvate, lacta...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)58097-2
更新日期：1996-02-01 00:00:00
abstract：OBJECTIVE:To assess the safety and effectiveness of a sprayable, site-specific adhesion barrier system (SprayGel; Confluent Surgical, Waltham, MA) for reduction of adhesion formation. DESIGN:Prospective, randomized, controlled phase III trial. SETTING:University clinic (Kiel, Germany) and private clinic (Bordeaux, Fr...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章,多中心研究,随机对照试验
doi：10.1016/j.fertnstert.2003.12.046
更新日期：2004-08-01 00:00:00
abstract：:The role of insulin sensitizing agents is discussed at length in this Committee Opinion. ...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/j.fertnstert.2008.08.004
更新日期：2008-11-01 00:00:00
abstract：OBJECTIVE:To assess the effect of age and ovarian function on endometrial receptivity. DESIGN:Retrospective comparison between standard IVF and ovum donation in younger and older patients (< 40 and > or = 40 years of age, respectively). PATIENTS:In standard IVF, there were 325 transfer cycles in older patients and 1,...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)56104-4
更新日期：1993-08-01 00:00:00
abstract：OBJECTIVE:To compare pregnancy rates (PRs) using blastocysts cryopreserved on day 7 with those cryopreserved on days 5 and 6. DESIGN:Retrospective observational cohort study. SETTING:Infertility center performing IVF. PATIENT(S):Eight hundred women with infertility undergoing frozen ET. INTERVENTION(S):Blastocysts ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2013.06.021
更新日期：2013-10-01 00:00:00
abstract：OBJECTIVE:To further understand the association between semen quality and cancer risk by means of well defined semen parameters. DESIGN:Retrospective cohort study. SETTING:Not applicable. PATIENT(S):A total of 20,433 men who underwent semen analysis (SA) and a sample of 20,433 fertile control subjects matched by age...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2015.10.027
更新日期：2016-02-01 00:00:00
abstract：:Peritoneal fluid and serum were collected from 78 patients at the time of laparoscopy. Twenty-two were fertile controls (CTL), and 56 were infertility patients, who were subdivided into three main groups: endometriosis (EMS), pelvic adhesions (ADH), and ovarian dysfunction (OvDF). Based on control group data, biochemi...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)48020-9
更新日期：1984-08-01 00:00:00
abstract：OBJECTIVE:To determine whether antisperm autoantibody production after prepubertal vas injury is influenced by immediate repair of the vas compared to delay of the reanastomosis until sexual maturity. DESIGN:Animal study comparing early repair, late repair, and sham-operated groups. SETTING:Research laboratory in a m...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(99)00501-4
更新日期：2000-02-01 00:00:00
abstract：:Blood was collected from 20 patients with varicocele (15 in the infertile group and 5 in the incidental varicocele group) at the time of high ligation by insertion of a catheter into the internal spermatic vein toward the renal side. Simultaneously, blood was obtained from the antecubital vein. The levels of cortisol,...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)46043-7
更新日期：1982-02-01 00:00:00
abstract：OBJECTIVE:To investigate adrenal steroid regulation in polycystic ovary syndrome. DESIGN:Five-hour oral glucose tolerance test (OGTT) and frequently sampled-intravenous gluclose tolerance test. SETTING:University research center. PATIENT(S):Thirty patients. INTERVENTION(S):None. MAIN OUTCOME MEASURE(S):Anthropomet...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2009.03.017
更新日期：2010-03-01 00:00:00
abstract：OBJECTIVE:To determine the incidence and type of mixoploidy in human blastocysts produced in vitro. DESIGN:A laboratory study of spare blastocysts from an IVF program. SETTING:University hospital laboratory. PATIENT(S):Thirty-nine couples undergoing IVF or intracytoplasmic sperm injection. INTERVENTION(S):A total o...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(02)04393-5
更新日期：2002-12-01 00:00:00
abstract：:In this prospective, randomized clinical trial, we tested the hypothesis that a needle-free CO(2) injection system (Biojector 2000) would be better tolerated by patients when compared with traditional sharp needles used for gonadotropin injections during stimulation for IVF. As measured by our questionnaire, the needl...
journal_title：Fertility and sterility
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.fertnstert.2009.03.013
更新日期：2009-10-01 00:00:00
abstract：OBJECTIVES:To investigate whether endometrial thickness on the day of hCG administration is a predictor of intrauterine insemination (IUI) success. DESIGN:A retrospective study. PATIENT(S):Two hundred forty-nine women undergoing IUI cycles. SETTING:University hospital-based infertility center. INTERVENTION(S):Endom...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2006.12.010
更新日期：2007-08-01 00:00:00
abstract：:Although the general trend for delaying childbearing is generally viewed as causing infertility, its consequences on the interpregnancy interval have been unknown. A study of birth records for Swiss married women from 1969 to 2006 revealed that the woman's age at first birth has increased from 25.0 to 30.1 years, wher...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2009.05.078
更新日期：2009-12-01 00:00:00
abstract：OBJECTIVE:To report our experience with genetic screening of oocyte donor candidates and to determine the frequency with which significant genetic issues are identified. DESIGN:Prospective genetic screening of oocyte donor candidates. SETTING:University hospital oocyte donation program. PATIENT(S):Women presenting c...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/s0015-0282(98)00109-5
更新日期：1998-07-01 00:00:00
abstract：:The pituitary response to luteinizing hormone-releasing hormone (LHRH) was tested in women before and approximately 2, 8, and 12 weeks after the injection of either Depo-Provera or norethisterone oenanthate. The secretion of both luteinizing hormone and follicle-stimulating hormone in response to LHRH was similar in t...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)43635-6
更新日期：1978-11-01 00:00:00
abstract：OBJECTIVE:To harmonize the collection of nonsurgical clinical and epidemiologic data relevant to endometriosis research, allowing large-scale collaboration. DESIGN:An international collaboration involving 34 clinical/academic centers and three industry collaborators from 16 countries on five continents. SETTING:In 20...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/j.fertnstert.2014.07.1244
更新日期：2014-11-01 00:00:00
abstract：OBJECTIVE:To determine efficacy and safety of asoprisnil in patients with leiomyomata. DESIGN:Phase 2, multicenter, prospective, randomized, double-blind, placebo-controlled, parallel-group study. SETTING:Twenty-eight sites in the United States and 1 in Canada. PATIENT(S):One hundred twenty-nine women with leiomyoma...
journal_title：Fertility and sterility
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1016/j.fertnstert.2006.11.094
更新日期：2007-06-01 00:00:00
abstract：:This report describes our initial experience with elective single blastocyst transfer in 19 patients who had a mean age of 36.3 +/- 2.4 years. The ongoing pregnancy rate, 53% after the fresh embryo transfer and 68% when thaw cycles are included, suggests that single blastocyst transfer has a place in this relatively o...
journal_title：Fertility and sterility
pub_type： 信件
doi：10.1016/j.fertnstert.2003.10.050
更新日期：2004-06-01 00:00:00
abstract：OBJECTIVE:To study the possible presence of extracellular superoxide anion (O2-) in untreated semen of normal and pathological subjects and to determine the possible relationship between superoxide anion levels, number of seminal leukocytes (WBCs) and seminal parameters. PATIENTS:Semen samples were analyzed from ferti...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)57017-4
更新日期：1994-10-01 00:00:00
abstract：OBJECTIVE:To study the ovarian reserve, ovarian response to gonadotropins, and oocyte maturity in women with cancer undergoing in vitro fertilization (IVF) before chemotherapy or radiotherapy. DESIGN:Case-control study. SETTING:University teaching hospital. PATIENT(S):We evaluated all women with malignancy who under...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2011.04.070
更新日期：2011-07-01 00:00:00
abstract：OBJECTIVE:To evaluate human sperm acrosomal status, zona pellucida (ZP)-binding capacity, and sperm motion characteristics after treatment with pertussis toxin followed by exposure to increasing concentrations of solubilized human ZP. DESIGN:Prospective analytical study. SETTING:Normal human sperm donors in an academ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1996-12-01 00:00:00
abstract：OBJECTIVE:To evaluate the effect of melatonin both on the ovaries of pinealectomized female rats through histomorphometric analysis and on steroid receptors, proliferating cell nuclear antigen (PCNA), and vascular endothelial growth factor (VEGF) expression. DESIGN:Experimental study. SETTING:Federal University of Sã...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2010.04.042
更新日期：2011-03-15 00:00:00
abstract：OBJECTIVE:To investigate c-fos and matrix metalloproteinase-9 (MMP-9) expression in the endometrium from women with or without endometriosis throughout the menstrual cycle, and to explore the correlation of c-fos expression with MMP-9 expression and 17beta-E(2) levels in serum. DESIGN:Molecular studies in human tissue...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2007.07.1386
更新日期：2008-10-01 00:00:00
abstract：OBJECTIVE:To evaluate how endometriosis affects expression of vascular endothelial growth factor (VEGF) and interleukin-6 (IL-6) in granulosa cells. DESIGN:Prospective study. SETTING:IVF-ET program at Osaka Medical College. PATIENT(S):Seventeen patients with revised American Fertility Society stage IV endometriosis ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(02)03339-3
更新日期：2002-10-01 00:00:00
