当前位置： SCI文献检索 > INFECTION AND IMMUNITY期刊下所有文献 > The iron-repressed, AraC-like regulator MpeR activates expression of fetA in Neisseria gonorrhoeae.

The iron-repressed, AraC-like regulator MpeR activates expression of fetA in Neisseria gonorrhoeae.

Abstract：

:Neisseria gonorrhoeae is an obligate human pathogen that causes the common sexually transmitted infection gonorrhea. Gonococcal infections cause significant morbidity, particularly among women, as the organism ascends to the upper reproductive tract, resulting in pelvic inflammatory disease, ectopic pregnancy, and infertility. In the last few years, antibiotic resistance rates have risen dramatically, leading to severe restriction of treatment options for gonococcal disease. Gonococcal infections do not elicit protective immunity, nor is there an effective vaccine to prevent the disease. Thus, further understanding of the expression, function, and regulation of surface antigens could lead to better treatment and prevention modalities in the future. In the current study, we determined that an iron-repressed regulator, MpeR, interacted specifically with the DNA sequence upstream of fetA and activated FetA expression. Interestingly, MpeR was previously shown to regulate the expression of gonococcal antimicrobial efflux systems. We confirmed that the outer membrane transporter FetA allows gonococcal strain FA1090 to utilize the xenosiderophore ferric enterobactin as an iron source. However, we further demonstrated that FetA has an extended range of substrates that encompasses other catecholate xenosiderophores, including ferric salmochelin and the dimers and trimers of dihydroxybenzoylserine. We demonstrated that fetA is part of an iron-repressed, MpeR-activated operon which putatively encodes other iron transport proteins. This is the first study to describe a regulatory linkage between antimicrobial efflux and iron transport in N. gonorrhoeae. The regulatory nidus that links these systems, MpeR, is expressed exclusively by pathogenic neisseriae and is therefore expected to be an important virulence factor.

journal_name

Infect Immun

journal_title

Infection and immunity

authors

Hollander A,Mercante AD,Shafer WM,Cornelissen CN

doi

10.1128/IAI.05806-11

subject

Has Abstract

pub_date

2011-12-01 00:00:00

pages

4764-76

issue

eissn

0019-9567

issn

1098-5522

pii

IAI.05806-11

journal_volume

pub_type

杂志文章

在线工具

The iron-repressed, AraC-like regulator MpeR activates expression of fetA in Neisseria gonorrhoeae.