Abstract：
BACKGROUND:Overexpression of breast cancer-specific gene 1 (SNCG) is associated with poor prognosis in advanced breast cancer patients. This study aimed to determine the effects of SNCG knockdown in breast cancer cells by using small hairpin RNA (shRNA). METHODS:Four different SNCG shRNA oligonucleotides were designed and chemically synthesized to construct mammalian expression vectors. These vectors were then stably transfected into a breast cancer MCF-7 cell line to knockdown SNCG expression. After SNCG knockdown was confirmed, the stable cell lines were inoculated into nude mice. SNCG mRNA and protein expressions were analyzed by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry, respectively in both the stable cell lines and xenografts. RESULTS:All four SNCG shRNA constructs significantly reduced SNCG mRNA and protein levels in MCF-7 cells, as compared to the unrelated sequence control shRNA and the liposome control mice (P < 0.05). SNCG-knockdown MCF-7 cells formed significantly smaller tumor masses than cells expressing the unrelated sequence control or the liposome control mice (P < 0.05). CONCLUSION:SNCG shRNA effectively suppressed breast cancer cell formation in vivo and may be a useful clinical strategy to control breast cancer.
journal_name
Chin Med J (Engl)journal_title
Chinese medical journalauthors
Shen PH,Fan QX,Li YW,Zhang W,He XK,Wang Z,Zhang YHsubject
Has Abstractpub_date
2011-05-01 00:00:00pages
1524-8issue
10eissn
0366-6999issn
2542-5641journal_volume
124pub_type
杂志文章abstract：BACKGROUND:With features of high tissue contrast, MRI can be used for the qualitative and quantitative evaluation of atherosclerosis plaques. In this study we investigated the development of atherosclerosis plaque with high resolution 3T MRI in a rabbit model and compared the findings with the histopathological results...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2012-08-01 00:00:00
abstract：OBJECTIVE:To investigate the short- and long-term clinician and angiographic outcome of stenting or angioplasty for chronic total coronary artery occlusion. METHODS:A total of 95 patients with successful percutaneous transluminal coronary angioplasty (PTCA) for total occlusion coronary artery were identified out of 35...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2000-03-01 00:00:00
abstract：:To develop a more comprehensive index for predicting the prognous of liver cirrhosis. 300 consecutive patients with cirrhosis were studied in terms of survival from 1975 to 1986. Median follow-up period was 5.3 years. A multivariable survival analysis (Cox's regression model) using clinical biochemical data obtained a...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1989-04-01 00:00:00
abstract：BACKGROUND:Sericin peptide (SP) has shown a powerful anti-oxidant property in a host of studies. The present study was designed to investigate the possible protective effects of SP against alcohol-induced gastric lesions in mice and to explore the potential mechanisms. METHODS:Animals were randomly divided into 5 grou...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2008-10-20 00:00:00
abstract：:This study demonstrates that there are distinct abnormalities of vitamin D and calcium metabolism in children with nephrotic syndrome (NS) and normal renal function. In patients with active NS, serum calcium, ionized calcium, 25-OHD3 and CT were decreased, plasma PTH level was increased and serum ALP was elevated. The...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1992-10-01 00:00:00
abstract：BACKGROUND:Morphologic imaging after radiofrequency ablation (RFA) of liver metastases is hampered by an inflammatory response in the ablation margin, making the identification of local tumor progression (LTP) difficult. The aim of this study was to evaluate the efficacy of early (18)F-FDG PET/CT scanning to monitor th...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2010-07-01 00:00:00
abstract：BACKGROUND:The primary reasons for local recurrence and therapeutic failure in the treatment of malignant gliomas are the invasion and interactions of tumor cells with surrounding normal brain cells. However, these tumor cells are hard to be visualized directly in histopathological preparations, or in experimental glio...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2012-12-01 00:00:00
abstract：BACKGROUND:Artificial joints present certain problems such as osteal absorption and lysis induced by wear debris which leads to loosening of the prosthesis over a period of time. Here we propose a design of an artificial magnetic suspension joint that was prepared by integrating the medical theories of modern material ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2010-12-01 00:00:00
abstract：OBJECTIVE:To analyze the effect and mechanism of hyperbaric oxygen (HBO) treatment for severe brain injury (SBI). METHODS:Fifty-five patients were divided into a treatment group of 35 patients and a control group of 20 patients. We observed the alterations of clinical, brain electric earth map (BEAM), endothelin (ET) ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2001-04-01 00:00:00
abstract：OBJECTIVE:Renal fibrosis is the most common manifestation of chronic kidney disease (CKD). Noting that existing treatments of renal fibrosis only slow disease progression but do not cure it, there is an urgent need to identify novel therapies. Hydrogen sulfide (H2S) is a newly discovered endogenous small gas signaling ...
journal_title：Chinese medical journal
pub_type： 杂志文章,评审
doi：10.1097/CM9.0000000000000537
更新日期：2019-12-05 00:00:00
abstract：BACKGROUND:Nucleostemin is essential for the proliferation and survival of stem and cancer cells, but it is unknown whether this newly identified molecule is involved in prostate cancer pathogenesis. METHODS:Total RNA and protein were extracted from prostate cancer tissues and PC-3, LNCap and DU145 cell lines. The nuc...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2008-02-20 00:00:00
abstract：OBJECTIVE:To characterize effects of the GP Ib alpha mutation (A156V) on its interaction with von Willebrand factor (vWf) under high fluid shear stress. METHODS:The residue A156 of GP Ib alpha was converted to a valine and the mutant expressed in CHO cells expressing wild-type GP Ib beta and GPIX. The transfected cell...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2000-08-01 00:00:00
abstract：BACKGROUND:Limited data exist on the association of serum gamma-glutamyl transferase (GGT) level within the reference range with the increased risk of coronary heart disease (CHD) prediction in men. The study examined the association between serum GGT concentration within the reference range and the CHD risk prediction...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.4103/0366-6999.161343
更新日期：2015-08-05 00:00:00
abstract：:A 30-year-old female patient with coexisting ankylosing spondylitis and rheumatoid arthritis was diagnosed and treated. The human leukocyte antigen (HLA)-B27 is a predisposing factor of ankylosing spondylitis and HLA-DR4 is a predisposing factor of rheumatoid arthritis. This patient was HLA-B27 and HLA-DR4 positive, a...
journal_title：Chinese medical journal
pub_type： 杂志文章,评审
doi：
更新日期：2011-10-01 00:00:00
abstract：BACKGROUND:It has been long suggested that abnormal clinical factors in the body, such as dyslipidemia and diabetes, can affect the presence of atherosclerosis. However, few studies on the effect of factors within the normal range, such as the loss of renal function with age, on the prevalence of atherosclerosis are fe...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2012-08-01 00:00:00
abstract：BACKGROUND:Clinical practice guidelines (CPGs) play an important role in healthcare in China as well as in the world. However, the current status and trends of Chinese CPGs are unknown. The aim of this study was to systematically review the present situation and the quality of Chinese CPGs published in the peer-reviewe...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2012-10-01 00:00:00
abstract：BACKGROUND:Transforming growth factor-beta1 (TGF-beta1) exerts strong fibrogenic potential in culture-activated HSCs. Smad4 is a key intracellular mediator for the transforming growth factor-beta (TGF-beta) superfamily of growth factors. The aim of this study was to assess the effects of the antisense Smad4 gene on Ito...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2004-08-01 00:00:00
abstract：OBJECTIVE:To review the relationship between personal behavior and cultural factors and reproductive tract infections (RTIs). METHODS:An English-language literature search using MEDLINE (1994-1998), Social Science Index (1994-1998), and informal discovery. Any original researches and reviews related to RTIs' epidemiol...
journal_title：Chinese medical journal
pub_type： 杂志文章,评审
doi：
更新日期：1999-11-01 00:00:00
abstract：OBJECTIVE:To explore the relationship between interleukin-8 (IL-8) and airway hyper-responsiveness (AHR) of asthma in guinea pigs. METHODS:IL-8 at a dose of 0.5 microgram/kg or 5.0 micrograms/kg was administered intranasally to guinea pigs twice a week for 3 weeks. 24 hours after the last administration, airway respon...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1999-11-01 00:00:00
abstract：OBJECTIVE:To evaluate whether hypermethylation of calcitonin (CT) gene could serve as a transforming signal of myelodysplastic syndrome (MDS) to leukemia. METHODS:Bone marrow aspirates from 35 MDS patients, including 25 refractory anemia (RA), 10 refractory anemia with excess of blasts (RAEB) or refractory anemia with...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1998-08-01 00:00:00
abstract：BACKGROUND:Cerebral ischemia-reperfusion injury is the main reason for the loss of neurons in the ischemic cerebrovascular disease. Therefore, to deeply understand its pathogenesis and find a new target is the key issue to be solved. This research aimed to investigate the neuroprotective effects of salvianolic acid B (...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2010-12-01 00:00:00
abstract：BACKGROUND:Severe acute respiratory syndrome (SARS) is a disease with a mortality of 9.56%. Although SARS is etiologically linked to a new coronavirus (SARS-CoV) and functional cell receptor has been identified, the pathogenesis of the virus infection is largely unclear. METHODS:The clinical specimens were processed a...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2008-04-05 00:00:00
abstract：OBJECTIVE:To study the experience in the treatment of subclavian steal syndrome (SSS) and the relation between SSS and the damage of brain. METHODS:In 28 patients with SSS, 23 were operated on. They complained of vertigo, TIA, and claudication of arms. One patient suffered from severe stem ischemia spasm. All the pati...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1999-11-01 00:00:00
abstract：BACKGROUND:Myocardial apoptosis is involved in the pathogenesis of sepsis-related myocardial depression. However, the underlying mechanism remains unknown. This study investigated the role of mitochondrial damage and mitochondria-induced oxidative stress during cardiac apoptosis in septic rats. METHODS:Seventy-two Spr...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2013-01-01 00:00:00
abstract：BACKGROUND:Early neurological deterioration (END) is a prominent issue after recanalization treatment. However, few studies have reported the characteristics of END after endovascular treatment (EVT) as so far. This study investigated the incidence, composition, and outcomes of END after intravenous recombinant tissue ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.4103/0366-6999.222343
更新日期：2018-01-20 00:00:00
abstract：OBJECTIVE:The objective was to provide a brief history of J wave syndromes and to summarize our current understanding of their molecular, ionic, cellular mechanisms, and clinical features. We will also discuss the existing debates and further direction in basic and clinical research for J wave syndromes. DATA SOURCES:...
journal_title：Chinese medical journal
pub_type： 杂志文章,评审
doi：10.4103/0366-6999.154320
更新日期：2015-04-05 00:00:00
abstract：OBJECTIVE:To induce islet grafting tolerance by intravenous injection of anti-CD4, anti-CD8 immunotoxins and donor soluble antigen. METHODS:Fourteen days or 7 days prior to transplantation, the immunotoxin of anti-CD4, anti-CD8 200 micrograms respectively, and donor soluble antigen 500 micrograms were intravenously in...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1999-12-01 00:00:00
abstract：BACKGROUND:The chronic obstructive pulmonary disease assessment test (CAT) is an easy to use health-related quality of life questionnaire, the modified Medical Research Council (mMRC) dyspnea scale is a classic dyspnea scale which is widely used, while the correlation between them is still not clear. This study investi...
journal_title：Chinese medical journal
pub_type： 杂志文章,多中心研究
doi：
更新日期：2013-01-01 00:00:00
abstract：BACKGROUND:Pelizaeus-Merzbacher disease (PMD) is a rare X-linked recessive disorder with symptoms including nystagmus, impaired motor development, ataxia, and progressive spasticity. The proteolipid protein 1 (PLP1) gene is the only pathogenic gene of PMD. Duplication of the PLP1 gene is the most frequent gene defect, ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2008-09-05 00:00:00
abstract：OBJECTIVE:To investigate the efficacy of continuous renal replacement therapy (CRRT) versus intermittent hemodialysis (IHD) in patients with severe acute renal failure (ARF). METHODS:One hundred and ninety-three severe ARF patients who received renal support between December 1978 and December 1998 were involved in thi...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2001-11-01 00:00:00
