Abstract：
OBJECTIVE:To evaluate whether hypermethylation of calcitonin (CT) gene could serve as a transforming signal of myelodysplastic syndrome (MDS) to leukemia. METHODS:Bone marrow aspirates from 35 MDS patients, including 25 refractory anemia (RA), 10 refractory anemia with excess of blasts (RAEB) or refractory anemia with excess of blasts in transformation (RAEBt) and 7 cases of acute myeloid leukemia (AML) transformed from MDS, were studied on methylation rate in 5' end of CT gene by polymerase chain reaction (PCR) technique using methylation-sensitive endonuclease Hpa II with external references of undigested DNA and Msp I digested DNA and internal reference of 112 bp fragment containing codon 61 of N-ras oncogene. The results were expressed as calcitonin gene methylation rate (CTMR) calculated from the densitometer-analyzed integral calculus of PCR products of 566 bp CT(a1), 112 bp N-ras(b1) by using Hpa II-digested DNA and PCR products of 566 bp CT (a0), 112 bp N-ras(b0) by using undigested DNA according to the formula, CTMR = (a1/b1)/(a0/b0) x 100%. RESULTS:The CTMRs in total 35 MDS, 25 RA, 10 RAEBt and 7 cases of AML transformed from MDS were 36.87% +/- 25.10%, 28.12% +/- 24.01%, 58.74% +/- 16.49%, and 54.03% +/- 7.06% respectively, significantly higher than that in control group (P < 0.001, P < 0.05, P < 0.001 and P < 0.001, respectively). CONCLUSION:The results suggest that hypermethylation of CT gene occurs in early stage of leukemic transformation and CTMR might be a useful marker in predicting the transformation of MDS to AML.
journal_name
Chin Med J (Engl)journal_title
Chinese medical journalauthors
Wu S,Xie G,Bai R,Wang Y,Zhu Pkeywords:
subject
Has Abstractpub_date
1998-08-01 00:00:00pages
690-3issue
8eissn
0366-6999issn
2542-5641journal_volume
111pub_type
杂志文章abstract：Background:Betel quid chewing has been a major risk factor for oral cancer (OC) in southern China. This study aimed to analyze the scientific publications on the relationship between betel quid chewing and OC and construct a model to quantitatively and qualitatively evaluate pertinent publications from 1998 to 2017. M...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.4103/0366-6999.238140
更新日期：2018-08-20 00:00:00
abstract：BACKGROUND:Four single nucleotide polymorphisms (SNPs) in the modulator recognition factor 2/AT-rich interaction domain 5B (MRF2/ARID5B) gene located at chromosome 10q21.2 have been shown to be associated with both type 2 diabetes mellitus (T2DM) and coronary artery disease in a Japanese cohort. This study aimed to inv...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.4103/0366-6999.204926
更新日期：2017-05-05 00:00:00
abstract：BACKGROUND:Single incision laparoscopic colectomy has been performed in recent years, and has been shown to be feasible and safe. This study was to assess the feasibility of single incision laparoscopic right hemicolectomy and to compare the differences in different approaches. METHODS:This retrospective study include...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2013-01-01 00:00:00
abstract：BACKGROUND:The 2019 novel coronavirus has caused the outbreak of the acute respiratory disease in Wuhan, Hubei Province of China since December 2019. This study was performed to analyze the clinical characteristics of patients who succumbed to and who recovered from 2019 novel coronavirus disease (COVID-19). METHODS:C...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.1097/CM9.0000000000000824
更新日期：2020-06-05 00:00:00
abstract：BACKGROUND:Transgenic overexpression of human prostasin in rats disturbs salt balance and causes hypertension. We investigated whether genetic variations in prostasin were implicated in hypertension or related phenotypes in the Xinjiang Kazakh population. METHODS:We sequenced all exons and the promoter regions of the ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2011-07-01 00:00:00
abstract：BACKGROUND:Previous studies have showed that photooxidative stress can lead to down-modulation of nuclear factor-kappa B (NF-kappaB) activity causing apoptosis of cultured photoreceptor cells. This study aimed at investigating whether NF-kappaB was involved in photoreceptor cells apoptosis induced by N-methyl-N-nitroso...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2005-07-05 00:00:00
abstract：OBJECTIVE:To assess the effects of Dy-DTPA-BMA (sprodiamide) on ex-vivo MR imaging of reperfused acute myocardial infarction. METHODS:Eighteen dogs were subjected to 2-hour coronary artery occlusion followed by 24-hour reperfusion. Dysprosium-chelate (Dy-DTPA-BMA) was injected into 16 dogs. Twenty minutes before their...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2000-03-01 00:00:00
abstract：BACKGROUND:The CYP2C19 G681A single polymorphism has been proven to affect clopidogrel responsiveness. However, the effect of coexisting polymorphisms of other genes has not yet been reported in the Chinese population. This study investigated the effect of coexisting polymorphisms of CYP2C19 and P2Y12 on clopidogrel re...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2013-03-01 00:00:00
abstract：BACKGROUND:There are no clear guidelines on implant removal. Few have assessed the long-term outcomes of patients with implants left in-situ, or removed. Therefore, removal of implants after fracture fixation remains controversial. METHODS:In this retrospective study, we reviewed 53 patients with implant for fracture ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2011-08-01 00:00:00
abstract：OBJECTIVE:To investigate the role of a potential diabetes-related mitochondrial region, which includes two previously reported mutations, 3243A-->G and 3316G-->A, in Chinese patients with adult-onset type 2 diabetes. METHODS:A total of 277 patients and 241 normal subjects were recruited for the study. Mitochondrial nt...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2002-05-01 00:00:00
abstract：BACKGROUND:Contrast induced acute kidney injury (CIAKI) is an important complication in the use of iodinated contrast media (CM). Our study was to evaluate the neutrophil gelatinase-associated lipocalin (NGAL) and cystatin C for early diagnosis of CIAKI. METHODS:The patients with established or suspected coronary arte...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2012-03-01 00:00:00
abstract：BACKGROUND:Atrial fibrillation (AF) is the most common supraventricular arrhythmia in clinical practice. Chronic atrial fibrillation (CAF) is associated with ionic remodeling. However, little is known about the activity of ATP-sensitive potassium current (IK, ATP) during CAF. So we studied the changes of IK, ATP densit...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2005-07-20 00:00:00
abstract：BACKGROUND:At present, revascularization is still one of the most critical technologies in orthotopic liver transplantation (OLT). Hepatic artery (HA) variations occur frequently in both donors and recipients. Moreover, there are always some pathological changes in the recipient hepatic artery. If handled improperly, i...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2011-10-01 00:00:00
abstract：BACKGROUND:Chronic liver disease causes aberrant formation of fibrous tissue that impedes normal liver function, ultimately resulting in liver cirrhosis. Iron uptake can occur within the hepatic parenchyma or within the various nodules that form in a cirrhotic liver, termed siderotic nodules (SN). We aimed to investiga...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2012-09-01 00:00:00
abstract：OBJECTIVE:To explore the association of human leukocyte antigen (HLA) with pregnancy induced hypertension (PIH). METHODS:We oligotyped HLA-DQA1, -DQB1 locus of 30 Chinese PIH families and 14 control families in Shanghai area by polymerase chain reaction-sequence specific oligonucleotide (PCR-SSO) hybridization method ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1998-02-01 00:00:00
abstract：OBJECTIVE:To study the preventive effect of artemether (Art) in protecting the people from schistosome infection during flood fighting in schistosomiasis endemic area of Poyang Lake, Jiangxi Province. METHODS:From mid July to mid August in 1996, the water level in Poyang Lake rose due to torrential rains and 2 embankm...
journal_title：Chinese medical journal
pub_type： 临床试验,杂志文章,随机对照试验
doi：
更新日期：1998-02-01 00:00:00
abstract：BACKGROUND:Cross-sectional area (CSA) for small pulmonary vessels is considered a parameter of pulmonary vessel alterations in patients with chronic obstructive pulmonary disease. This study was to evaluate the correlation of CSA with airflow obstruction parameters in asthma. Furthermore, we aimed to measure the differ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.1097/CM9.0000000000000367
更新日期：2019-08-20 00:00:00
abstract：:This study evaluated the diagnostic value of high resolution CT (HRCT) for atresia of the external auditory meatus (EAM) and isolated middle ear malformation. CT scan of the temporal bone was done in 33 patients with such disorders and 5 patients with otosclerosis etc. were studied in the same way for comparison. Coro...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1992-06-01 00:00:00
abstract：BACKGROUND:Although acute congestive heart failure (CHF) patients typically present with abnormal auscultatory findings on lung examination, lung sounds are not normally subjected to rigorous analysis. The goals of this study were to use a computerized analytic acoustic tool to evaluate lung sound patterns in CHF patie...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2010-05-05 00:00:00
abstract：BACKGROUND:Our previous research has suggested that genes around D12S1056 in 12q13 may confer susceptibility to ventricular septal defect (VSD) in humans. The present study was to define the chromosome region assignment by transmission disequilibrium test (TDT), and to identify the important candidate gene by family-ba...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2006-02-20 00:00:00
abstract：BACKGROUND:Cerebral ischemia-reperfusion injury is the main reason for the loss of neurons in the ischemic cerebrovascular disease. Therefore, to deeply understand its pathogenesis and find a new target is the key issue to be solved. This research aimed to investigate the neuroprotective effects of salvianolic acid B (...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2010-12-01 00:00:00
abstract：BACKGROUND:During cardiac arrest, the gastrointestinal tract is sensitive to ischemia. Protection of the gastrointestinal tract is a critical factor in determining prognosis following cardiopulmonary resuscitation (CPR). This study seeks to determine the extent of gastrointestinal tract injury and the potential protect...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2012-12-01 00:00:00
abstract：OBJECTIVE:To determine whether mutation of the coding or junction region of glucokinase gene (GCK) is also the pathogenic cause of the common form of non-insulin-dependent diabetes mellitus (NIDDM) in Chinese. METHODS:Single strand conformation polymorphism (SSCP) analysis was performed after the 12 exons and junction...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1996-11-01 00:00:00
abstract：BACKGROUND:This study was designed to evaluate the relationship between high-density lipoprotein cholesterol (HDL-C) level and acute myocardial infarction (AMI) and coronary heart disease (CHD) death and to explore the protective effect of HDL against CHD in the elderly Chinese. METHODS:Started from 1986, 1211 retiree...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2004-04-01 00:00:00
abstract：OBJECTIVE:To investigate molecular events of exocytosis in cultured human pheochromocytoma cells with stimulation. METHODS:The cultured pheochromocytoma cells prepared from human adrenal pheochromocytoma tumor were stimulated for the release of catecholamines by depolarization with the administration of 50 mmol/L KCl....
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1998-04-01 00:00:00
abstract：BACKGROUND:OX40/OX40 ligand (OX40/OX40L) and programmed death-1/programmed death ligand-1 (PD-1/PD-L1) costimulatory signals play important roles in T cell-induced immune responses. The aim of this study was to investigate the roles of OX40/OX40L and PD-1/PD-L1 costimulatory pathways in mouse islet allograft rejection....
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
abstract：:In present study, we examined the effects of NG-nitro-L-arginine (LNNA), an inhibitor of nitric oxide synthase (NOS), and/or methylene blue (MB), a blocker of guanylate cyclase on the vasodilator response of isolated rat arteries including aorta and mesenteric artery to calcitonin gene-related peptide (CGRP) by in vit...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1994-10-01 00:00:00
abstract：BACKGROUND:Hamstring (HS) autograft and bone-patellar tendon-bone allograft are the most common choice for reconstruction of anterior cruciate ligament (ACL). There was a little report about the clinical outcome and difference of arthroscopic ACL reconstruction using allograft and autograft. This study aimed to compare...
journal_title：Chinese medical journal
pub_type： 杂志文章,随机对照试验
doi：10.4103/0366-6999.170265
更新日期：2015-12-05 00:00:00
abstract：BACKGROUND:The growing enthusiasm for coronary artery bypass grafting (CABG) without cardiopulmonary bypass (CPB) is emerging, but the role of off-pump coronary artery bypass (OPCAB) in clinical practice remains controversial. The purpose of this study was to assess differences in the incidences of stroke, atrial fibri...
journal_title：Chinese medical journal
pub_type： 杂志文章,meta分析
doi：
更新日期：2012-01-01 00:00:00
abstract：:Anti-HNK-1 monoclonal antibody (MAb) was reactive with non-cancerous and cancerous prostatic epithelia, as well as natural killer cells, myelinated nerves and cells from APUD systems. The expression of HNK-1 antigen on prostate cancer was investigated immunohistochemically by avidinbiotin-peroxidase complex (ABC) meth...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1995-07-01 00:00:00
