Abstract：
OBJECTIVE:To investigate the role of a potential diabetes-related mitochondrial region, which includes two previously reported mutations, 3243A-->G and 3316G-->A, in Chinese patients with adult-onset type 2 diabetes. METHODS:A total of 277 patients and 241 normal subjects were recruited for the study. Mitochondrial nt 3116 - 3353, which spans the 16S rRNA, tRNA(leu(UUR)) and the NADH dehydrogenase 1 gene, were detected using polymerase chain reaction (PCR), direct DNA sequencing, PCR-restriction fragment length polymorphism and allele-specific PCR. Variants were analyzed by two-tailed Fisher exact test. The function of the variants in 16S rRNA were predicted for minimal free energy secondary structures by RNA folding software mfold version 3. RESULTS:Four homoplasmic nucleotide substitutions were observed, 3200T-->C, 3206C-->T, 3290T-->C and 3316G-->A. Only the 3200T-->C mutation is present in the diabetic population and absent in the control population. No statistically significant associations were found between the other three variants and type 2 diabetes. The 3200T-->C and 3206C-->T nucleotide substitutions located in 16S rRNA are novel variants. The 3200T-->C caused a great alteration in the minimal free energy secondary structure model while the 3206C-->T altered normal 16S rRNA structure little. CONCLUSIONS:The results suggest that the 3200T-->C mutation is linked to the development of type 2 diabetes, but that the other observed mutations are neutral. In contrast to the Japanese studies, the 3316G-->A does not appear to be related to type 2 diabetes.
journal_name
Chin Med J (Engl)journal_title
Chinese medical journalauthors
Yang T,Lam CW,Tsang MW,Tong SF,Kam GY,Chan LY,Poon PM,Wu X,Pang CPkeywords:
subject
Has Abstractpub_date
2002-05-01 00:00:00pages
753-8issue
5eissn
0366-6999issn
2542-5641journal_volume
115pub_type
杂志文章abstract：OBJECTIVE:Dermoscopy is a useful technique for improving the diagnostic accuracy of various types of skin disorders. In China, dermoscopy has been widely accepted, and domestic researchers have made tremendous progress in the field of dermoscopy. The main purpose of this review is to summarize the current status of der...
journal_title：Chinese medical journal
pub_type： 杂志文章,评审
doi：10.1097/CM9.0000000000000396
更新日期：2019-09-05 00:00:00
abstract：OBJECTIVE:To investigate the status of the drug resistance and the ampC gene expression of Enterobacter cloacae. METHODS:Disk diffusion tests were made for detecting the susceptibility of antimicrobial agents against Enterobacter cloacae. AmpC gene was amplified by polymerase chain reaction (PCR) and verified by DNA s...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2003-08-01 00:00:00
abstract：BACKGROUND:Survivin is an oncoprotein silenced in normal mature tissues but reactivated in serous ovarian cancer (SOC). Although transcriptional activation is assumed for its overexpression, the long 3'-untranslated region (3'-UTR) in survivin gene, which contains many alternate polyadenylation (APA) sites, implies a p...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.4103/0366-6999.181965
更新日期：2016-05-20 00:00:00
abstract：BACKGROUND:Visfatin, a visceral fat-derived adipocytokine, plays a significant physiological function in lipid metabolism. However, the precise function of visfatin and its regulation by thyroid hormones are still unknown. This study observed the plasma visfatin concentrations in subjects with hyperthyroidism and hypot...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2012-03-01 00:00:00
abstract：BACKGROUND:Several difficulties can arise from wide-neck cerebral aneurysms when treated with endovascular embolization. We aimed to investigate the effect of endovascular treatment of intracranial aneurysms using coil embolization plus an Enterprise stent. METHODS:Forty patients were treated with coil embolization pl...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2011-02-01 00:00:00
abstract：BACKGROUND:The effectiveness of neoadjuvant chemoradiotherapy (NCRT) treatment for patients with esophageal carcinoma (EC) remains controversial. The aim of this study was to compare the effect of NCRT followed by surgery (NCRTS) with surgery alone (SA) for EC. METHODS:The PubMed, EMBASE, and the Cochrane Library data...
journal_title：Chinese medical journal
pub_type： 杂志文章,meta分析
doi：10.4103/0366-6999.195464
更新日期：2016-12-20 00:00:00
abstract：OBJECTIVE:To investigate the role of bax in a vincristine (VCR)-induced multidrug-resistant (MDR) human gastric cancer cell line, SGC7901/VCR, in which the Bax protein expression level was significantly lower compared with that in parent cells. METHODS:A bax eukaryotic expression vector was constructed and transfected...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2000-11-01 00:00:00
abstract：OBJECTIVE:To study the expression of vascular endothelial growth factor (VEGF) in different parts of heart during early acute myocardial ischemia. METHODS:The expression of VEGF in control and study group in rats was performed with the Strept Avidin-Biotin-Peroxidase Complex (SABC) method. RESULTS:A rapid expression ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1999-08-01 00:00:00
abstract：OBJECTIVE:To evaluate the safety and immunogenicity of lyophilized, live attenuated hepatitis A vaccine (H2 strain) in rhesus monkeys. METHODS:Nine adult rhesus monkeys were used as experimental animals. The rhesus monkeys without anti-HAV were divided randomly into the aqueous vaccination group (4 rhesus monkeys), th...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2000-04-01 00:00:00
abstract：OBJECTIVE:To investigate the antitumor immune efficiency of mouse dendritic cells (mDCs) by using adenovirus-mediated tumor necrosis factor-alpha (AdV-TNF-alpha) gene transfer. METHODS:MDCs infected with AdV-TNF-alpha and AdV-pLpA (no gene insert) at 100 multiplicity of infection (MOI) were analyzed by RNase protectio...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2002-12-01 00:00:00
abstract：BACKGROUND:In tumors the process of apoptosis occurs over an interval of time after chemotherapy. It is important to determine the best time for detecting apoptosis by in vivo imaging. In this study, we evaluated the dynamics and feasibility of imaging non-small cell lung cancer (NSCLC) apoptosis induced by paclitaxel ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2013-01-01 00:00:00
abstract：BACKGROUND:Both cortical and cortical-subcortical (cortex-involved) lesions are typically associated with embolic stroke, of which atrial fibrillation (AF) is the common cause. The aim of this study was to find out the associations between cortex-involved stroke, vascular risk factors, and the subtypes (discovery time ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.1097/CM9.0000000000000390
更新日期：2019-09-05 00:00:00
abstract：OBJECTIVE:To assess the value of noninvasive three-dimensional computed tomographic angiography (3D-CTA) in preoperative detection of intracranial arteriovenous malformations (AVMs). METHODS:A prospective evaluation at a single institute over a 2-year period included 23 patients suspected of intracranial AVMs. All pat...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2000-10-01 00:00:00
abstract：BACKGROUND:In county-level tuberculosis (TB) dispensaries in China, the accurate diagnosis of sputum smear-negative pulmonary tuberculosis (SNPT) needs to be improved by developing and validating clinical and radiographic predictors. METHODS:The study was conducted simultaneously in three counties per province in Chon...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2013-01-01 00:00:00
abstract：OBJECTIVE:To study the effect of pericardial devascularization combined with Latarjet's innervation on portal hypertension. METHODS:Forty-eight patients undergoing pericardial devascularization combined with Latarjet's innervation were compared with 57 patients with devascularization. Clinical results were evaluated b...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1998-12-01 00:00:00
abstract：OBJECTIVE:To explore the mechanism of autoimmune hemolysis and establish a more sensitive test for autoimmune hemolytic anemia (AIHA). METHODS:IgG subclasses were tested in 40 patients with idiopathic or secondary AIHA by Coombs test with monoclonal antibodies. Hb, TBil, RC and FHb, as hemolytic parameters, were used ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1999-09-01 00:00:00
abstract：BACKGROUND:The side effects of cyclosporine therapy include thromboembolic complications. However, the mechanisms underlying the hypercoagulable state induced by cyclosporine are not fully understood. Cyclosporine binds to red blood cells (RBCs) with a high affinity in circulation and alters the membranes of RBCs. Ther...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2009-07-20 00:00:00
abstract：BACKGROUND:Lumbar spinal stenosis is a common problem that is receiving attention with the advent of novel treatment procedures. Prior positional MRI studies demonstrated lumbar canal diameter changes with flexion and extension. There have not been any studies to examine the amount of spinal canal diameter change relat...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2010-06-01 00:00:00
abstract：:Four calcium channel blockers, i.e. nifedipine, verapamil, cinnarizine and tetrandrine are currently available and used widely in treating cardiovascular diseases. To confirm the effects, if any, of calcium channel blockers on cirrhotic patients with portal hypertension, a study was performed on esophageal variceal pr...
journal_title：Chinese medical journal
pub_type： 临床试验,杂志文章,随机对照试验
doi：
更新日期：1995-11-01 00:00:00
abstract：BACKGROUND:Septicemia and inflammation-mediated septic shock caused by Vibrio vulnificus (VV) is strongly associated with chronic liver disease. This study examined the effects of antimicrobial therapy on expression of hepatic toll-like receptors and inflammatory cytokines in rats with alcohol-induced liver disease com...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2009-08-20 00:00:00
abstract：BACKGROUND:Fluid dynamic mechanisms attributed to coronary bifurcation lesions remain a subject of study. The present study aimed at investigating the hemodynamic change of wall shear stress (WSS) in patients with coronary bifurcation lesions treated by double kissing (DK) crush or one-stent with final kissing balloon ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2012-05-01 00:00:00
abstract：BACKGROUND:Myocardial bridging with systolic compression of the left anterior descending coronary artery (LAD) may be associated with myocardial ischaemia. The clinical outcome in patients with surgical treatment for symptomatic myocardial bridging remains undetermined. This study assessed the middle- and long-term res...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2007-09-20 00:00:00
abstract：OBJECTIVE:To explore the management of cerebral ischemia caused by Takayasu's arteritis. METHODS:Ninety-three cases treated from June 1984 to September 1999 at the General Post & Telecom Hospital, the Sir Run Run Shaw Hospital, the First Affiliated Hospital of Zhejiang University, the Second Medical College of Beijing...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2002-03-01 00:00:00
abstract：BACKGROUND:The offspring of women with gestational diabetes mellitus (GDM) are prone to macrosomia. However, birth weight is difficult to be correctly estimated by ultrasound because of fetal asymmetric growth characteristics. This study aimed to investigate the correlations between fetal hemodynamics, fetal growth ind...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.4103/0366-6999.189057
更新日期：2016-09-05 00:00:00
abstract：BACKGROUND:The most typical cardiac abnormality is conotruncal defects (CTDs) in patients with 22q11 deletion syndrome (22q11DS). HIRA (histone cell cycle regulator) gene, as one of the candidate genes located at the critical region of 22q11DS, was reported as possibly relevant to CTD in animal models. This study aimed...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.4103/0366-6999.191745
更新日期：2016-10-20 00:00:00
abstract：BACKGROUND:In the past 5 years, new treatment materials and techniques offering a different concept in endovascular treatment have been described for cerebral arteriovenous malformations (CAVMs). The aim of this study was to assess the endovascular treatment of CAVMs by using a liquid embolic material, Onyx (Micro Ther...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2005-12-20 00:00:00
abstract：:A study was made in Neijiang City, Sichuan Province to observe the safety and immunogenicity of a live attenuated hepatitis A virus (HAV) vaccine (H2 strain). 3,031 children, aged 6 to 9 years, were injected subcutaneously with 10(6.0) TCID50, 10(5.5) TCID50, and 10(5.0) TCID50 of HAV vaccine; the seroconversion rate ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1993-08-01 00:00:00
abstract：BACKGROUND:Oxygen inhalation therapy is essential for the treatment of patients with chronic mountain sickness (CMS), but the efficacy of oxygen inhalation for populations at high risk of CMS remains unknown. This research investigated whether oxygen inhalation therapy benefits populations at high risk of CMS. METHODS...
journal_title：Chinese medical journal
pub_type： 杂志文章,随机对照试验
doi：10.4103/0366-6999.182831
更新日期：2016-06-05 00:00:00
abstract：BACKGROUND:Chronic liver diseases are a major burden in China. Alanine aminotransferase (ALT) can be used as an indicator of hepatocyte damage. In this study, we determined the prevalence and etiologies of elevated ALT in an adult population in Jilin, China. METHODS:A total of 4072 individuals aged between 18 and 79 y...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2011-09-01 00:00:00
abstract：BACKGROUND:Total knee arthroplasty (TKA) may be associated with serious complications that adversely affect outcomes and increase the likelihood of disability. However, few studies with sufficient sample size have reported postoperative complications following TKA among Chinese patients. This study aimed to evaluate co...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.4103/0366-6999.213071
更新日期：2017-11-05 00:00:00
