Abstract:
BACKGROUND:Cerebral ischemia-reperfusion injury is the main reason for the loss of neurons in the ischemic cerebrovascular disease. Therefore, to deeply understand its pathogenesis and find a new target is the key issue to be solved. This research aimed to investigate the neuroprotective effects of salvianolic acid B (SalB) against oxygen-glucose deprivation/reperfusion (OGD/RP) damage in primary rat cortical neurons. METHODS:The primary cultures of neonatal Wister rats were randomly divided into the control group, the OGD/RP group and the SalB-treatment group (10 mg/L). The cell model was established by depriving of oxygen and glucose for 3 hours and reperfusion for 3 hours and 24 hours, respectively. The neuron viability was determined by MTT assay. The level of cellular reactive oxygen species (ROS) was detected by fluorescent labeling method and spin trapping technique respectively. The activities of neuronal Mn-superoxide dismutase (Mn-SOD), catalase (CAT) and glutathione peroxidase (GSH-PX) were assayed by chromatometry. The mitochondria membrane potential (ΔΨ(m)) was quantitatively analyzed by flow cytometry. The release rate of cytochrome c was detected by Western blotting. The neuronal ultrastructure was observed by transmission electron microscopy. Statistical significance was evaluated by analysis of variance (ANOVA) followed by Student-Newman-Keuls test. RESULTS:OGD/RP increased the level of cellular ROS, but decreased the cell viability and the activities of Mn-SOD, CAT and GSH-PX; SalB treatment significantly reduced the level of ROS (P < 0.05); and enhanced the cell viability (P < 0.05) and the activities of these antioxidases (P < 0.05). Additionally, OGD/RP induced the fluorescence value of ΔΨ(m) to diminish and the release rate of cytochrome c to rise notably; SalB markedly elevated the level of ΔΨ(m) (P < 0.01) and depressed the release rate of cytochrome c (P < 0.05); it also ameliorated the neuronal morphological injury. CONCLUSION:The neuroprotection of SalB may be attributed to the elimination of ROS and the inhibition of apoptosis.
journal_name
Chin Med J (Engl)journal_title
Chinese medical journalauthors
Wang Y,Jiang YF,Huang QF,Ge GL,Cui Wsubject
Has Abstractpub_date
2010-12-01 00:00:00pages
3612-9issue
24eissn
0366-6999issn
2542-5641journal_volume
123pub_type
杂志文章abstract:BACKGROUND:This retrospective study aimed to investigate the difference of the intra-dural reserving space for spinal cord in magnetic resonance imaging (MRI) between patients with and without developmental cervical stenosis and its clinical significance. METHODS:A total of 264 patients with cervical spondylotic myelo...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
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doi:
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.4103/0366-6999.213959
更新日期:2017-09-20 00:00:00
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journal_title:Chinese medical journal
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更新日期:2005-07-20 00:00:00
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更新日期:2004-04-01 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1998-08-01 00:00:00
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更新日期:2010-09-01 00:00:00
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更新日期:2013-02-01 00:00:00
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pub_type: 杂志文章
doi:
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2010-01-05 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章,评审
doi:
更新日期:1999-02-01 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2000-11-01 00:00:00
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pub_type: 杂志文章,随机对照试验
doi:
更新日期:2009-03-20 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2000-09-01 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2010-10-01 00:00:00
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journal_title:Chinese medical journal
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:1990-02-01 00:00:00
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journal_title:Chinese medical journal
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doi:
更新日期:2000-01-01 00:00:00
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doi:
更新日期:1997-04-01 00:00:00
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pub_type: 杂志文章
doi:
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doi:
更新日期:2007-12-20 00:00:00
abstract:: ...
journal_title:Chinese medical journal
pub_type: 信件
doi:10.4103/0366-6999.243565
更新日期:2018-10-20 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章,多中心研究
doi:
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doi:
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doi:
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