Abstract：
BACKGROUND:Severe acute respiratory syndrome (SARS) is a disease with a mortality of 9.56%. Although SARS is etiologically linked to a new coronavirus (SARS-CoV) and functional cell receptor has been identified, the pathogenesis of the virus infection is largely unclear. METHODS:The clinical specimens were processed and analyzed using an indirect enzyme-linked immunosorbent assay (ELISA) in-house. Further investigations of target antigen included reviews of phage display technique, rapid amplification of cDNA ends (RACE) technique, protein expression and purification, Western blotting validation, serological and immunohistochemical staining in postmortem tissue. RESULTS:A type of medium or low titer anti-lung tissue antibodies were found in the sera of SARS patients at the early stage of the disease. Human long interspersed nuclear element 1 (LINE1) gene endonuclease (EN) domain protein was one of the target autoantigens and it was aberrantly expressed in the lung tissue of SARS patients. Anti-EN antibody was positive in the sera of 40.9% of SARS patients. CONCLUSIONS:Human LINE1 endonuclease domain was identified as a putative target of SARS-associated autoantibodies, which were presented in the serum of SARS patients and may be involved in the pathogenesis of SARS.
journal_name
Chin Med J (Engl)journal_title
Chinese medical journalauthors
He WP,Shu CL,Li BA,Zhao J,Cheng Ysubject
Has Abstractpub_date
2008-04-05 00:00:00pages
608-14issue
7eissn
0366-6999issn
2542-5641journal_volume
121pub_type
杂志文章abstract：OBJECTIVE:To study protective immunity afforded by murine immunization with DNA vaccine of Schistosoma japonicum (S. japonicum) as measured by reduction in worm burden and host antibody, cytokines. METHODS:DNA vaccine pCD-Sj32 was constructed, identified and expressed. pCD-Sj32 could induce substantial protective immu...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
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journal_title：Chinese medical journal
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abstract：BACKGROUND:Cholesterol-lowering therapy with statins has been reported to reduce the morbidity and mortality of cardiovascular diseases. This study aimed to investigate the effects of combined application of extended-release niacin and atorvastatin on lipid profile modification and the risks of adverse events in patien...
journal_title：Chinese medical journal
pub_type： 杂志文章,随机对照试验
doi：
更新日期：2009-07-20 00:00:00
abstract：BACKGROUND:Nasopharyngeal carcinoma (NPC) is a squamous-cell carcinoma especially prevailing among the natives of southern China. The regimen of concurrent chemoradiotherapy (CCRT) that include platinum and 5-fluorouracil (5-FU) is considered to be the standard treatment for NPC. However, its clinical use is limited by...
journal_title：Chinese medical journal
pub_type： 杂志文章,meta分析
doi：
更新日期：2014-01-01 00:00:00
abstract：BACKGROUND:Acute kidney injury (AKI) is a severe disease in critically ill patients. Neutrophil infiltration into kidney was associated with the development of AKI, and P-selectin may be involved in the process of neutrophil recruitment in kidney. This study aimed to explore the potential effect of platelet-derived P-s...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.4103/0366-6999.209889
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abstract：: ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.4103/0366-6999.244110
更新日期：2018-11-05 00:00:00
abstract：OBJECTIVE:A human promyelocytic leukemia (APL) cell line NB4 was used to demonstrate the synergistic effects between all-trans retinoic acid (ATRA) and prostaglandin E2 (PGE2) on growth inhibition and cytodifferentiation induction. METHODS:NB4 cells were cultured in the presence of either ATRA or PGE2 as a single agen...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1997-10-01 00:00:00
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journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2007-03-05 00:00:00
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journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1994-06-01 00:00:00
abstract：OBJECTIVE:To investigate the correlation between glutathione S-transferase (GST) M1 and T1 genotypes and endometriosis risk (EM). METHODS:Polymerase chain reaction (PCR) technique was used to detect the presence or absence of the GSTM1 and GSTT1 genes in genomic DNA isolated from the blood samples of 68 Han Chinese wo...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2003-05-01 00:00:00
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journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2008-09-05 00:00:00
abstract：BACKGROUND:HIV-1 infected and immune-activated macrophages and microglia secrete neurotoxins, such as tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β), which play major role in the neuronal death. It has been shown that different HIV-1 variants have varying abilities to elicit secretion of TNF-α by peripheral...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2011-12-01 00:00:00
abstract：BACKGROUND:Anomalous origin of the left coronary artery from the pulmonary artery (ALCAPA) is a rare but potentially life-threatening congenital heart defect. A retrospective analysis was carried out to elucidate the surgical outcomes of ALCAPA in infants and children using follow-up echocardiography. METHODS:From Sep...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.4103/0366-6999.215334
更新日期：2017-10-05 00:00:00
abstract：BACKGROUND:Stereotactic radiosurgery is an alternative to resection of intracranial cerebral arteriovenous malformations (AVMs), while it will fail in some cases. This study aimed to evaluate the changes after stereotactic radiosurgery for AVMs. METHODS:Nineteen cases with cerebral AVMs had failure after stereotactic ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2008-06-20 00:00:00
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journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2009-05-05 00:00:00
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journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2012-03-01 00:00:00
abstract：Background:Signal transducer and activator of transcription 3 (STAT3) was strongly expressed and activated in psoriatic keratinocytes (KCs) and correlated with the severity of psoriasis. The study aimed to investigate the effects of STAT3 small interfering RNA (siRNA) combined with ultrasonic irradiation and SonoVue mi...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.4103/0366-6999.239297
更新日期：2018-09-05 00:00:00
abstract：OBJECTIVE:To reveal the sequence of events of osteoclastic resorption taking place in the cortical bone of the femoral neck in aged women. MATERIAL AND METHODS:The cross sections of cortical bones of the femoral neck from 7 aged women who underwent endoprosthetic replacement for intracapsular hip fracture, were proces...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1996-09-01 00:00:00
abstract：BACKGROUND:The mechanisms underlying postoperative pain remain unclear. Neurotransmitters of excitatory and inhibitory amino acids play an important role in the transmission and modulation of pain in the spinal dorsal horn. This study aimed to investigate the changes of release of excitatory and inhibitory amino acids ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2007-11-20 00:00:00
abstract：BACKGROUND:Inner retinal oxygenation response (ΔPO(2)) is a worldwide study focus. However, the relevant reports on its radiological measurements are limited. In this study, magnetic resonance imaging (MRI), employing T1 weighted image (T1WI), was used to detect changes in ΔPO(2) following 100% oxygen inhalation in hum...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2010-11-01 00:00:00
abstract：:The effects of hypoxic endothelial cell conditioned medium (HECCM) on proliferation and collagen synthesis of cultured porcine pulmonary arterial smooth muscle cells (PASMCs) were studied by 3H-thymidine (3H-TdR) and 3H-proline incorporations, image analysis for determination of DNA content and colorimetric assay usin...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1995-11-01 00:00:00
abstract：OBJECTIVE:To have a better understanding of genetic contributions to the development of obstructive sleep apnea hypopnea syndrome (OSAHS) by reviewing studies on its genetic basis. DATA SOURCES:A comprehensive search of the PubMed literature without restriction on the publication date was carried out using terms "obst...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
abstract：BACKGROUND:In patients with chronic total occlusion (CTO) and multivessel coronary artery disease, the comparison of surgical and the percutaneous revascularization strategies has rarely been conducted. The aim of this study was to compare long term clinical outcomes of drug eluting stent (DES) implantation with corona...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2011-04-01 00:00:00
abstract：BACKGROUND:One of the major causes of death in severe acute pancreatitis (SAP) is severe infection owing to bacterial translocation. Some clinical studies suggested that ecoimmunonutrition (EIN) as a new strategy had better treatment effect on SAP patients. But the experiment studies on the precise mechanism of the eff...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2006-04-20 00:00:00
abstract：BACKGROUND:Double plate osteosynthesis is the standard treatment for intra-articular fractures of the distal humerus. However, there is still controversy concerning dual plate positions in terms of providing optimal stability. The purpose of this retrospective study was to compare the clinical outcomes in patients with...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2013-06-01 00:00:00
abstract：BACKGROUND:The conventional method cannot guarantee the precise osteotomies required for a perfect realignment and a better prognosis after total knee arthroplasty (TKA). This study investigated a customized guide plate for osteotomy placement in TKAs with the aid of the statistical shape model technique using weight-b...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.4103/0366-6999.176082
更新日期：2016-02-20 00:00:00
abstract：BACKGROUND:Laparoscopic Heller cardiomyotomy and Dor fundoplication is the surgical procedure of choice for esophageal achalasia. The aim of this study was to investigate the clinical outcome of laparoscopic Heller-Dor procedure in our initial series of 25 patients with achalasia. METHODS:Between October 2003 and Janu...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2006-03-20 00:00:00
abstract：:Endometriosis is a prevalent chronic disease that affects approximately 6% to 10% of reproductive-aged women. Although numerous researchers have endeavored to explore the etiology of endometriosis over a century, its etiology still remains an enigma. The exploration of pathophysiologic mechanism and novel therapy for ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.1097/CM9.0000000000000885
更新日期：2020-07-20 00:00:00
abstract：BACKGROUND:Hippophae rhamnoides L. (HL) exerts antioxidant activities against various oxidative stress conditions. In this study, we investigated effects of extract from HL leaves (HLE) on cell proliferation and neuroblast differentiation in the subgranular zone (SGZ) of the dentate gyrus (DG) of aged gerbils. METHODS...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
abstract：BACKGROUND:Roux-en-Y gastric bypass (GBP) is the main surgical procedure used in type 2 diabetes. The objective of this study was to evaluate the different types of GBP in treatment of type 2 diabetes. METHODS:Patients with type 2 diabetes were randomly divided into two groups: those who underwent gastrojejunal loop a...
journal_title：Chinese medical journal
pub_type： 杂志文章,随机对照试验
doi：
更新日期：2012-06-01 00:00:00
