Protective effect of heme oxygenase-1 on lung injury induced by erythrocyte instillation in rats.

Abstract:

BACKGROUND:Intratracheal instillation of blood induces self-repaired acute lung injury. However, the mechanism of repair has been unclear. Heme-oxygenase (HO)-1, which catalyzes heme breakdown, acts as an inducible defense against oxidative stress and plays an important role in inflammation. The objective of this study was to test the role of HO-1 in lung injury caused by intratracheal instillation of red cells. METHODS:Forty healthy, male Sprague-Dawley rats were randomly divided into five groups: normal group, saline group, erythrocyte group, erythrocyte+zinc-protoporphyrin (ZnPP, HO-1 inhibitor) group and saline+ZnPP group. At 2 days after intratracheal instillation of red cells, lung tissues and lavage samples were isolated for biochemical determinations and histological measurements. RESULTS:Histological analysis revealed that administration of ZnPP worsened the acute lung injury induced by instilled erythrocytes. HO-1 was over-expressed in the erythrocyte group and in the erythrocyte + ZnPP group. Compared with the erythrocyte + ZnPP group, the levels of total protein, lactate dehydrogenase and tumor necrosis factor-alpha in the lavage were lower (P < 0.01), while the level of interleukin-10 was higher in the erythrocyte group (P < 0.01). CONCLUSION:HO-1 protects against erythrocyte-induced inflammatory injury in lung.

journal_name

Chin Med J (Engl)

journal_title

Chinese medical journal

authors

Pang QF,Zhou QM,Zeng S,Dou LD,Ji Y,Zeng YM

subject

Has Abstract

pub_date

2008-09-05 00:00:00

pages

1688-92

issue

17

eissn

0366-6999

issn

2542-5641

journal_volume

121

pub_type

杂志文章