Expression of the c-Met Proteins in Malignant Skin Cancers.

Abstract:

BACKGROUND:The expression of c-Met is substantially elevated in most malignant human cancers. We therefore searched for c-Met expression and compared the expression level among malignant skin cancers. OBJECTIVE:The aim of this study was to determine the c-Met expression pattern and the protein expression level in selected malignant cutaneous tumors. METHODS:G361 cells (malignant melanoma cell line) and A431 cells (squamous cell carcinoma cell line) were cultured and analyzed, using immunoprecipitation and Western blot analysis, for expression of c-Met. Additionally, 16 separate specimens of malignant melanomas (MMs), 16 squamous cell carcinomas (SCCs), 16 basal cell carcinomas (BCCs) and 16 normal tissues were analyzed for the expression of c-Met using immunohistochemical studies. RESULTS:Based on cultured cell immunoprecipitation and Western blot analysis, both A431 cells and G361 cells expressed c-Met, however, c-Met was expressed substantially more in G361 cells. Immunohistochemical examination of c-Met showed that it was over-expressed in all malignant skin cancers. In addition, c-Met expression was more increased in MM compared to other cancers. CONCLUSION:In our study, c-Met is involved in malignant skin cancer development and the level of its expression is thought to be related to the degree of malignancy in melanoma cancers.

journal_name

Ann Dermatol

journal_title

Annals of dermatology

authors

Lee YJ,Kim DH,Lee SH,Kim DW,Nam HS,Cho MK

doi

10.5021/ad.2011.23.1.33

subject

Has Abstract

pub_date

2011-02-01 00:00:00

pages

33-8

issue

1

eissn

1013-9087

issn

2005-3894

journal_volume

23

pub_type

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