当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Ergodic and nonergodic processes coexist in the plasma membrane as observed by single-molecule tracking.

Ergodic and nonergodic processes coexist in the plasma membrane as observed by single-molecule tracking.

Abstract：

:Diffusion in the plasma membrane of living cells is often found to display anomalous dynamics. However, the mechanism underlying this diffusion pattern remains highly controversial. Here, we study the physical mechanism underlying Kv2.1 potassium channel anomalous dynamics using single-molecule tracking. Our analysis includes both time series of individual trajectories and ensemble averages. We show that an ergodic and a nonergodic process coexist in the plasma membrane. The ergodic process resembles a fractal structure with its origin in macromolecular crowding in the cell membrane. The nonergodic process is found to be regulated by transient binding to the actin cytoskeleton and can be accurately modeled by a continuous-time random walk. When the cell is treated with drugs that inhibit actin polymerization, the diffusion pattern of Kv2.1 channels recovers ergodicity. However, the fractal structure that induces anomalous diffusion remains unaltered. These results have direct implications on the regulation of membrane receptor trafficking and signaling.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Weigel AV,Simon B,Tamkun MM,Krapf D

doi

10.1073/pnas.1016325108

subject

Has Abstract

pub_date

2011-04-19 00:00:00

pages

6438-43

issue

16

eissn

0027-8424

issn

1091-6490

pii

1016325108

journal_volume

108

pub_type

杂志文章

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