Abstract:
:Genetic analysis has revealed that the accumulation of several chloroplast mRNAs of the green alga Chlamydomonas reinhardtii requires specific nucleus-encoded functions. To gain insight into this process, we have cloned the nuclear gene encoding the Mbb1 factor by genomic rescue of a mutant specifically deficient in the accumulation of the mRNAs of the psbB/psbT/psbH chloroplast transcription unit. Mbb1 is a soluble protein in the stromal phase of the chloroplast. It consists of 662 amino acids with a putative chloroplast-transit peptide at its N-terminal end. A striking feature is the presence of 10 tandemly arranged tetratricopeptide-like repeats that account for half of the protein sequence and are thought to be involved in protein-protein interactions. The Mbb1 protein seems to have a homologue in higher plants and is part of a 300-kDa complex that is associated with RNA. This complex is most likely involved in psbB mRNA processing, stability, and/or translation.
journal_name
Proc Natl Acad Sci U S Aauthors
Vaistij FE,Boudreau E,Lemaire SD,Goldschmidt-Clermont M,Rochaix JDdoi
10.1073/pnas.97.26.14813keywords:
subject
Has Abstractpub_date
2000-12-19 00:00:00pages
14813-8issue
26eissn
0027-8424issn
1091-6490pii
97/26/14813journal_volume
97pub_type
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