Abstract:
:Leptin plays a key role in obstructive sleep apnea syndrome (OSAS). Leptin production in human airways has been previously evaluated by measuring leptin concentration in the exhaled breath condensate and in the induced sputum. The aim was to study leptin expression in the cells of induced sputum and in exhaled breath condensate of subjects with OSAS. Moreover, leptin concentrations in the blood were measured in the same groups of subjects. We enrolled four groups of patients: (1) obese patients with OSAS (OO); (2) non-obese patients with OSAS (NOO); (3) obese patients without OSAS (ONO); and (4) non-obese subjects without OSAS (C). Leptin expression was evaluated by immunocytochemistry in the sputum cells of the enrolled subjects. The concentrations of leptin in the exhaled breath condensate and plasma were measured by using a specific enzyme immunoassay. Leptin protein expression and the percentage of macrophages and neutrophils expressing leptin were higher in the induced sputum of OO, NOO and ONO patients than in C. Leptin concentrations in the exhaled breath condensate were significantly higher in OO patients (5.12 (3.8-6.6) ng ml(-1)) than in NOO (4.1 (3.9-5.2) ng ml(-1)) and ONO (4.2 (3.6-5.0) ng ml(-1)) patients. The concentration of leptin in plasma was significantly more elevated in OO (36 (24-65.9) ng ml(-1)) than in NOO (30.2 (12.4-51.4) ng ml(-1)), whereas it was not significantly different in ONO patients. This study showed that leptin in sputum and in the exhaled breath condensate is higher in obese patients with OSAS than in obese subjects without OSAS. Moreover, different mechanisms for determining leptin concentrations in the exhaled breath condensate and the blood are suggested.
journal_name
J Breath Resjournal_title
Journal of breath researchauthors
Carpagnano GE,Resta O,Pergola GD,Sabato R,Foschino Barbaro MPdoi
10.1088/1752-7155/4/3/036003subject
Has Abstractpub_date
2010-09-01 00:00:00pages
036003issue
3eissn
1752-7155issn
1752-7163pii
S1752-7155(10)58039-Xjournal_volume
4pub_type
杂志文章abstract::Reactive oxygen species are associated with tissue inflammation and injury. Our laboratory has demonstrated that ethane, a stable product of lipid peroxidation, in exhaled breath can be used to measure total body oxidative stress. Herein patients were studied who underwent outpatient surgery, laproscopic bilateral tub...
journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7155/3/1/016002
更新日期:2009-03-01 00:00:00
abstract::Proton transfer reaction time-of-flight mass spectrometry (PTR-ToF-MS) represents an attractive tool for the real-time analysis of VOC profiles in human breath. Quantification of breath VOCs by means of direct MS may be affected by the matrix, as human breath not only contains several hundred VOCs at the ppbV-pptV lev...
journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7163/aa9eea
更新日期:2018-03-01 00:00:00
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journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7155/7/2/026005
更新日期:2013-06-01 00:00:00
abstract:BACKGROUND:The analysis of volatile organic compounds (VOCs) in breath allows non-invasive investigations of diseases. Animal studies are conducted as a model to perform research of VOCs and their relation to diseases. In large animal models ruminants were often used as experimental targets. The effect of their physiol...
journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7163/aabdaf
更新日期:2018-05-14 00:00:00
abstract::Oxidative stress is associated with numerous health conditions and disorders, and aldehydes are known biomarkers of oxidative stress that can be non-invasively measured in exhaled human breath. Few studies report breath aldehyde levels in human populations, and none claim participant numbers in the hundreds or more. F...
journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7163/ab8865
更新日期:2020-07-21 00:00:00
abstract::Bronchiectasis, bronchiolectasis, and bronchiolisation of alveolar regions are salient features of idiopathic pulmonary fibrosis (IPF). We asked whether IPF was associated with physiological changes consistent with increases in the volume of conducting airways, and whether airway volume was related to the severity of ...
journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7155/10/1/016005
更新日期:2016-02-01 00:00:00
abstract::Microbial cultures and/or microbial associated diseases often have a characteristic smell. Volatile organic compounds (VOCs) are produced by all microorganisms as part of their normal metabolism. The types and classes of VOC produced is wide, including fatty acids and their derivatives (e.g. hydrocarbons, aliphatic al...
journal_title:Journal of breath research
pub_type: 杂志文章,评审
doi:10.1088/1752-7155/6/2/024001
更新日期:2012-06-01 00:00:00
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journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7155/1/1/014004
更新日期:2007-09-01 00:00:00
abstract::Chronic pulmonary infections are the principal cause of morbidity and mortality in individuals with cystic fibrosis (CF). Due to the polymicrobial nature of these infections, the identification of the particular bacterial species responsible is an essential step in diagnosis and treatment. Current diagnostic procedure...
journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7155/10/2/026008
更新日期:2016-04-27 00:00:00
abstract::In the present longitudinal study, we followed volatile organic compounds (VOCs) excreted in exhaled breath of 20 healthy individuals over time, while adhering to a gluten-free diet for 4 weeks prior to adherence to a normal diet. We used gas chromatography coupled with mass spectrometry (TD-GC-tof-MS) in combination ...
journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7155/7/3/037104
更新日期:2013-09-01 00:00:00
abstract:BACKGROUND:Recent studies have highlighted the possible involvement of leptin in inflammation. The leptin receptor is also expressed by alveolar macrophages, T lymphocytes and bronchial epitelial cells, suggesting a possible role in the cascade of airway inflammation. OBJECTIVES:The aim of the study was to evaluate th...
journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7163/aa61c5
更新日期:2017-09-27 00:00:00
abstract::Since the ancient discovery of the 'sweet odor' in human breath gas, pursuits of the breath analysis-based disease diagnostics have never stopped. Actually, the 'smell' of the breath, as one of three key disease diagnostic techniques, has been used in Eastern-Medicine for more than three thousand years. With advanceme...
journal_title:Journal of breath research
pub_type: 历史文章,杂志文章,评审
doi:10.1088/1752-7155/7/3/037109
更新日期:2013-09-01 00:00:00
abstract::In this article, a database of blood:air and fat:air partition coefficients (λ b:a and λ f:a) is reported for estimating 1678 volatile organic compounds recently reported to appear in the volatilome of the healthy human. For this purpose, a quantitative structure-property relationship (QSPR) approach was applied and a...
journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7155/10/1/017103
更新日期:2016-01-27 00:00:00
abstract::The objective of this study is to evaluate tonsil odor as a contributor to halitosis and to study the reduction of oral malodor by mouth rinsing, without tonsil treatment. In 48 halitosis patients, tonsil odor and oral malodor were assessed through the 0-5 scale. In tonsil odor assessment, a dental burnisher was inser...
journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7155/2/1/017016
更新日期:2008-03-01 00:00:00
abstract::Exhaled nitric oxide (eNO) has been suggested to be a marker of small airway injury. We investigated the effects of different ventilation strategies on eNO. Sixty-nine patients who received elective open abdominal surgery under general anesthesia with more than 2 h of surgery duration were randomly divided into three ...
journal_title:Journal of breath research
pub_type: 杂志文章,随机对照试验
doi:10.1088/1752-7155/9/1/016006
更新日期:2015-02-26 00:00:00
abstract::This study investigated whether oral malodour with plaque-related periodontal disease was associated with the prevalence of periodontopathic bacteria in saliva and tongue debris. The subjects were 30 patients who visited the Breath Odour Clinic. An oral examination was performed at baseline and two months after period...
journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7155/2/1/017006
更新日期:2008-03-01 00:00:00
abstract::Concentrations of exhaled volatile organic compounds (VOCs) may depend not only on biochemical or pathologic processes but also on physiological parameters. As breath sampling may be done in different body positions, effects of the sampling position on exhaled VOC concentrations were investigated by means of real-time...
journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7155/9/4/047105
更新日期:2015-11-19 00:00:00
abstract::The lung just like all other organs is affected by age. The lung matures by the age of 20 and age-related changes start around middle age, at 40-50 years. Exhaled nitric oxide (FENO) has been shown to be age, height and gender dependent. We hypothesize that the nitric oxide (NO) parameters alveolar NO (CANO), airway f...
journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7163/aa7957
更新日期:2017-09-13 00:00:00
abstract::Lung cancer is a leading cause of cancer-related deaths worldwide, and is considered one of the most aggressive human cancers, with a 5 year overall survival of 10-15%. Early diagnosis of lung cancer is ideal; however, it is still uncertain as to what technique will prove successful in the systematic screening of high...
journal_title:Journal of breath research
pub_type: 杂志文章,评审
doi:10.1088/1752-7155/10/3/034001
更新日期:2016-07-05 00:00:00
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journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7155/2/3/037009
更新日期:2008-09-01 00:00:00
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journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7163/ab1faf
更新日期:2019-06-25 00:00:00
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journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7155/10/3/036009
更新日期:2016-07-22 00:00:00
abstract::Fractional exhaled nitric oxide (FENO) is a marker of airway inflammation. Measuring FENO at multiple flow rates enables calculation of NO parameters: bronchial NO output (J awNO), bronchial wall (C awNO) and alveolar (C ANO) NO concentrations, and bronchial diffusion factor of NO (D awNO). FENO is known to rapidly re...
journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7163/abc054
更新日期:2020-11-05 00:00:00
abstract::Exhaled breath acetone (BrAce) was investigated during and after submaximal aerobic exercise as a volatile biomarker for metabolic responsiveness in high and lower-fit individuals in a prospective cohort pilot-study. Twenty healthy adults (19-39 years) with different levels of cardiorespiratory fitness (VO2peak), dete...
journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7163/abba6c
更新日期:2020-10-31 00:00:00
abstract::The discovery of nitric oxide (NO) as a signalling and regulatory molecule and its subsequent detection in the exhaled breath has not only yielded new mechanistic insights but also diagnostic opportunities and therapeutic targets in several important medical conditions. In diseases involving chronic pulmonary inflamma...
journal_title:Journal of breath research
pub_type: 杂志文章,评审
doi:10.1088/1752-7155/7/1/017118
更新日期:2013-03-01 00:00:00
abstract::Exhaled breath is coming to the forefront of non-invasive biomarker discovery efforts. Concentration of exhaled breath volatile organic compounds (VOCs) on thermal desorption (TD) tubes with subsequent analysis by gas chromatography-mass spectrometry (GC-MS) has dominated this field. As discovery experimentation incre...
journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7155/10/4/046008
更新日期:2016-10-12 00:00:00
abstract::Selected volatile organic compounds (VOCs) in breath may be considered biomarkers if they are indicative of distinct diseases or disease states. Given the inherent molecular selectivity of vibrational spectroscopy, infrared sensing technologies appear ideally suitable for the determination of endogenous VOCs in breath...
journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7155/8/2/026003
更新日期:2014-06-01 00:00:00
abstract::Volatile organic compounds (VOCs) in exhaled breath originate from current or previous environmental exposures (exogenous compounds) and internal metabolic (anabolic and catabolic) production (endogenous compounds). The origins of certain VOCs in breath presumed to be endogenous have been proposed to be useful as prec...
journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7155/7/1/017107
更新日期:2013-03-01 00:00:00
abstract::Breath contains hundreds of volatile organic compounds (VOCs), the composition of which is altered in a wide variety of diseases. Bacteria are implicated in the formation of VOCs, but the biochemical mechanisms that lead to the formation of breath VOCs remain largely hypothetical. We hypothesized that bacterial DNA fr...
journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7163/10/4/047103
更新日期:2016-12-17 00:00:00
abstract::Nitrative stress is thought to be involved in the inflammatory process in COPD airways, and the alveolar nitric oxide concentration (CAlv) has been reported to be increased. However, the CAlv levels are also regulated by gas diffusion at alveolar sites. The aim of the study was to assess the relationship between the C...
journal_title:Journal of breath research
pub_type: 杂志文章
doi:10.1088/1752-7155/7/4/046002
更新日期:2013-12-01 00:00:00