Abstract：
:Narcotic analgesics are widely (ab) used and sometimes only occur in low concentrations in biological samples. Therefore, a highly sensitive liquid chromatography tandem mass spectrometry method was developed for simultaneous analysis of 9 narcotic analgesics and metabolites (buprenorphine, O-desmethyltramadol, fentanyl, norbuprenorphine, norfentanyl, pethidine, piritramide, tilidine and tramadol) in urine and whole blood. Sample preparation was performed on a mixed-mode cation exchange solid phase extraction cartridge with an additional alkaline wash step to decrease matrix effects and thus increase sensitivity. Ionization with electrospray ionization was found to be more efficient than atmospheric pressure chemical ionization. The use of a mobile phase of high pH resulted in higher electrospray ionization signals than the conventional low pH mobile phases. In the final method, gradient elution with 10mM ammonium bicarbonate (pH 9) and methanol was performed on a small particle column (Acquity C18, 1.7 μm, 2.1 mm × 50 mm). Selectivity, matrix effects, recovery, linearity, sensitivity, precision, accuracy and stability were validated in urine and whole blood. All parameters were successfully evaluated and the method showed very high sensitivity, which was the major aim of this study. The applicability of the method was demonstrated by analysis of several forensic cases involving narcotic analgesics.
journal_name
Forensic Sci Intjournal_title
Forensic science internationalauthors
Verplaetse R,Tytgat Jdoi
10.1016/j.forsciint.2011.01.047subject
Has Abstractpub_date
2012-02-10 00:00:00pages
136-45issue
1-3eissn
0379-0738issn
1872-6283pii
S0379-0738(11)00062-4journal_volume
215pub_type
杂志文章abstract：:Cannabis cultivation in order to produce drugs is forbidden in Switzerland. Thus, law enforcement authorities regularly ask forensic laboratories to determinate cannabis plant's chemotype from seized material in order to ascertain that the plantation is legal or not. As required by the EU official analysis protocol th...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2010.03.034
更新日期：2010-07-15 00:00:00
abstract：:Previous studies have indicated that metal particles remain on bone after sharp force injury or gunshot and that their detection by scanning electron microscopy with energy dispersive X-ray spectrometry (SEM-EDS) could greatly help in tool identification. However, the presence of metal particles on bone surfaces in th...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2012.08.007
更新日期：2012-11-30 00:00:00
abstract：:The forensic anthropologist is responsible for contributing to the identification of an unknown by constructing a biological profile from their skeletal remains. Towards achieving this goal, anthropologists can apply population and temporally specific standards with known error margins to morphometric data collected f...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2014.08.019
更新日期：2014-11-01 00:00:00
abstract：:Household products such as bleach, gasoline or hydrochloric acid have been used to mask the presence of a cadaver or to prevent the colonization of insects. These types of chemicals affect insect development and alter the forensic entomology analysis. This study was designed to test the effects of six household produc...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2015.05.032
更新日期：2015-08-01 00:00:00
abstract：:The location of a gunshot entrance wound as well as the wound path trajectory are the main findings that help to determine the position from which a weapon was fired and thus distinguish firearm suicides from homicides and accidents. We present a case of a 28-year old man, who was found dead in his car. Because of an ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2017.03.015
更新日期：2017-06-01 00:00:00
abstract：:This report highlights the importance of an individual chemical's odor impact in the olfactory identification of marijuana, cocaine, and heroin. There are small amounts of highly odorous compounds present in headspace of these drugs, with very low odor detection thresholds, that are more likely responsible for contrib...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2015.08.027
更新日期：2015-12-01 00:00:00
abstract：:Laser-induced breakdown spectroscopy (LIBS), X-ray fluorescence spectroscopy (XRF) and scanning electron microscopy/energy dispersive X-ray spectroscopy (SEM/EDX) are compared in terms of their discrimination power when applied to Australian soil specimens. SEM/EDX and XRF are frequently used in forensic laboratories ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2014.10.018
更新日期：2014-12-01 00:00:00
abstract：OBJECTIVE:Age estimation in adults is an important issue in forensic science. This study aimed to estimate the chronological age of Iranians by means of pulp/tooth area ratio (AR) of canines in digital panoramic radiographs. MATERIALS AND METHODS:The sample consisted of panoramic radiographs of 271 male and female sub...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2018.01.016
更新日期：2018-04-01 00:00:00
abstract：:Driving under the influence of alcohol (DUIA) and drugs (DUID) is considered an elevated risk for traffic safety. When assessing a driver's fitness to drive, standardized and objective measurement methods are still required, in order to clarify the question whether an individual is under the influence of substances ac...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2020.110559
更新日期：2020-12-01 00:00:00
abstract：:The use of average curvature measurements for the forensic comparison of curly hairs has been reported, but a method, in which various types of hair form are quantitatively examined and objectively interpreted for hair comparison, has not been reported to date. In the present study, numerical data on hair form from Ja...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/s0379-0738(02)00450-4
更新日期：2003-01-28 00:00:00
abstract：:This report describes the heat-induced alterations in human bone tissue observed using scanning electron microscopy and microradiography. Femoral bone samples were taken from persons varying in age from 1 year to 97 years at the time of their death. The bone was heated at selected temperatures in the range 200-1600 de...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(95)01735-2
更新日期：1995-06-30 00:00:00
abstract：:Two rates of carbon dioxide flow into the termination chamber were applied for the euthanasia of rats. The classical phases of asphyxia were observed in both groups of animals although their course was prolonged in rats exposed to the lower gas flow. The haemorrhages were assessed histologically in the following struc...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/s0379-0738(01)00591-6
更新日期：2001-12-27 00:00:00
abstract：:The spectrophotometric method for the determination of carboxyhemoglobin (HbCO) in blood reported by Fretwurst and Meinecke was modified so as to give the same values of percentage HbCO (HbCO%) as those determined by the oxygen electrode method. Values of HbCO% of nine practical samples determined by both the oxygen e...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(81)90157-2
更新日期：1981-09-01 00:00:00
abstract：:We describe an experiment to investigate the potential effectiveness of an international DNA database for linking unsolved crimes using the data from a collaborative exercise among members of the European DNA Profiling Group. We show that effective retrieval of unknown samples will be feasible even from large database...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(92)90131-f
更新日期：1992-02-01 00:00:00
abstract：:Despite the fact that the differences in epidermal ridge density between men and women have been accepted for some time, they have only been thoroughly demonstrated in a small number of populations. The aim of this study is to determine whether such differences exist in a sample of the Spanish population by counting e...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2008.06.014
更新日期：2008-08-25 00:00:00
abstract：:Clinical and research-based tests in molecular biology require a substantial amount of DNA and RNA, unfortunately, a considerable number of cells is needed for this amount of sample. Blood is one of the best and easiest source of cells, but is not used due to its invasive drawing methods and the needed volume. Another...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2019.109931
更新日期：2019-10-01 00:00:00
abstract：:A 79-year-old woman suffered from acute posttraumatic stress disorder (PTSD) and a loco typico, non-displaced fracture of her right distal radius due to an incident involving the assault of two unleashed owned dogs, which suddenly ran into her and aggressively jumped on her chest and knocked her down to the ground. Re...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2011.02.016
更新日期：2011-05-20 00:00:00
abstract：:Cranial suture closure has been recognized for over a century as a useful trait for age estimation. Although this indicator has become a standard feature of age assessment protocols in skeletal remains, serious questions have been raised about its reliability. This article attempts to provide a comprehensive review of...
journal_title：Forensic science international
pub_type： 历史文章,杂志文章,评审
doi：10.1016/j.forsciint.2019.110111
更新日期：2020-02-01 00:00:00
abstract：:The aim of this work was to study the efficiency of automatic fibre searching with the Maxcan fibre finder (Cox Analytical Systems, Sweden) in comparison to manual searching. The influence of some parameters (color, thickness, background noise) on the results of a fibre search was considered. Eighteen experimental tap...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2005.08.015
更新日期：2006-07-13 00:00:00
abstract：:Nyaope is a mixture of low grade heroin, cannabis products, antiretroviral drugs and other materials added as bulking agents. It is a highly physically additive mixture which is smoked by users. As part of the development of a method for the analysis and profiling of nyaope this study evaluates the stability of the ca...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2019.04.040
更新日期：2019-07-01 00:00:00
abstract：:In this article, the authors present a case of intrauterine foetal death (IUFD). The post-mortem histologic examination revealed placental mesenchymal dysplasia (PMD), a rare human placental disorder. Moreover, cases of PMD are often misdiagnosed as partial mole. The mother was a 26-year-old Italian, whose pregnancy, ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2013.04.002
更新日期：2013-06-10 00:00:00
abstract：:Two rapid and reliable electrophoretic techniques for PGM1 and EsD typing on ultrathin polyacrylamide gels are described. They have been based on non-equilibrium pH gradient electrophoresis and on the addition of chemical spacers (EPPS for PGM1 and HEPES for EsD) to the gel mixture. ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(89)90197-7
更新日期：1989-07-01 00:00:00
abstract：:Sex estimation is an integral aspect of biological anthropology. Correctly estimating sex is the first step to many subsequent analyses, such as estimating living stature or age-at-death. Klales et al. (2012) [6] provided a revised version of the Phenice (1969) [3] method that expanded the original three traits (ventr...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2017.05.001
更新日期：2017-08-01 00:00:00
abstract：:5F-MDMB-PICA has been detected in products sold on the internet as well as in biological samples since 2016. It is associated with serious adverse health and behavioral effects and even death. Herein we report on twelve cases with proven 5F-MDMB-PICA consumption, including three fatalities, four cases of driving under...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2020.110410
更新日期：2020-09-01 00:00:00
abstract：:The ability of human pelvic bones to sexually differentiate has been of great interest in forensic anthropology for quite some time as it allows for the determination of skeletal sex by combining metric and morphological data. However, the criteria for determining the sex of a skeleton must be calibrated according to ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2012.06.014
更新日期：2012-10-10 00:00:00
abstract：:During a stabbing, apparel fabrics are usually damaged and may be penetrated. Despite numerous studies considering forces required to penetrate skin and human stabbing performance, none have systematically evaluated which variables affect severance appearance from a textile science perspective using a human stabbing p...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2015.01.024
更新日期：2015-04-01 00:00:00
abstract：:A 39-year-old woman was found dead at home. She was single, foreign national, chronic alcoholic, and had given shelter to a compatriot for three days. This man found the deceased in her bed, after a night of heavy drinking. The emergency medical team observed numerous recent blue ecchymoses of the upper limbs. Because...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2011.08.004
更新日期：2012-01-10 00:00:00
abstract：:This paper describes a sensitive UPLC-MS/MS method for quantification of methadone and 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) in single empty puparial case of Lucilia sericata. Larvae were reared on substrates spiked with different concentrations of methadone (0-4 μg/g). Methadone was quantified in p...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2011.01.020
更新日期：2011-06-15 00:00:00
abstract：:Our aim was to investigate the reason for relatively low detection rates for opioids and fentanyl in particular in post-mortem cases in the State of Hamburg. We re-analysed 822 blood samples from two different time periods, 2011/12 and 2016. These samples had been previously analysed in accordance with post-mortem rou...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2019.109970
更新日期：2019-12-01 00:00:00
abstract：:A very serious case of DFSA (drug facilitated sexual assault) is presented, in which a six-year-old girl died following sedation with γ-hydroxybutyric acid (GHB). She had been sexually abused by a relative. Samples of cardiac blood, bile, vitreous humour, liver, kidney, brain tissues and hair were analysed by a LC-MS/...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2015.12.044
更新日期：2016-02-01 00:00:00
