Abstract:
:This paper describes a sensitive UPLC-MS/MS method for quantification of methadone and 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) in single empty puparial case of Lucilia sericata. Larvae were reared on substrates spiked with different concentrations of methadone (0-4 μg/g). Methadone was quantified in puparia reared on high concentrated substrates (0.8-4 μg/g). The major metabolite of methadone (EDDP) was not detected, confirming rapid elimination of metabolites by the larvae before pupation. The effects of methadone on the development of L. sericata were also investigated. No effect on sex ratio was detected. A significant difference was calculated for emerged adults but no trends could be observed. Concerning the developmental curve, a significant difference was observed between control and high methadone concentrations using the Kolmogorov-Smirnov test.
journal_name
Forensic Sci Intjournal_title
Forensic science internationalauthors
Gosselin M,Di Fazio V,Wille SM,Fernandez Mdel M,Samyn N,Bourel B,Rasmont Pdoi
10.1016/j.forsciint.2011.01.020subject
Has Abstractpub_date
2011-06-15 00:00:00pages
154-9issue
1-3eissn
0379-0738issn
1872-6283pii
S0379-0738(11)00033-8journal_volume
209pub_type
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