Methadone determination in puparia and its effect on the development of Lucilia sericata (Diptera, Calliphoridae).

Abstract:

:This paper describes a sensitive UPLC-MS/MS method for quantification of methadone and 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) in single empty puparial case of Lucilia sericata. Larvae were reared on substrates spiked with different concentrations of methadone (0-4 μg/g). Methadone was quantified in puparia reared on high concentrated substrates (0.8-4 μg/g). The major metabolite of methadone (EDDP) was not detected, confirming rapid elimination of metabolites by the larvae before pupation. The effects of methadone on the development of L. sericata were also investigated. No effect on sex ratio was detected. A significant difference was calculated for emerged adults but no trends could be observed. Concerning the developmental curve, a significant difference was observed between control and high methadone concentrations using the Kolmogorov-Smirnov test.

journal_name

Forensic Sci Int

authors

Gosselin M,Di Fazio V,Wille SM,Fernandez Mdel M,Samyn N,Bourel B,Rasmont P

doi

10.1016/j.forsciint.2011.01.020

subject

Has Abstract

pub_date

2011-06-15 00:00:00

pages

154-9

issue

1-3

eissn

0379-0738

issn

1872-6283

pii

S0379-0738(11)00033-8

journal_volume

209

pub_type

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