Abstract:
:Platelets play an essential role in hemostasis and atherothrombosis. Owing to their short storage time, there is constant demand for this life-saving blood component. In this study, we report that it is feasible to generate functional megakaryocytes and platelets from human embryonic stem cells (hESCs) on a large scale. Differential-interference contrast and electron microscopy analyses showed that ultrastructural and morphological features of hESC-derived platelets were indistinguishable from those of normal blood platelets. In functional assays, hESC-derived platelets responded to thrombin stimulation, formed microaggregates, and facilitated clot formation/retraction in vitro. Live cell microscopy demonstrated that hESC-platelets formed lamellipodia and filopodia in response to thrombin activation, and tethered to each other as observed in normal blood. Using real-time intravital imaging with high-speed video microscopy, we have also shown that hESC-derived platelets contribute to developing thrombi at sites of laser-induced vascular injury in mice, providing the first evidence for in vivo functionality of hESC-derived platelets. These results represent an important step toward generating an unlimited supply of platelets for transfusion. Since platelets contain no genetic material, they are ideal candidates for early clinical translation involving human pluripotent stem cells.
journal_name
Cell Resjournal_title
Cell researchauthors
Lu SJ,Li F,Yin H,Feng Q,Kimbrel EA,Hahm E,Thon JN,Wang W,Italiano JE,Cho J,Lanza Rdoi
10.1038/cr.2011.8subject
Has Abstractpub_date
2011-03-01 00:00:00pages
530-45issue
3eissn
1001-0602issn
1748-7838pii
cr20118journal_volume
21pub_type
杂志文章相关文献
CELL RESEARCH文献大全abstract::Ferroptosis is an iron-dependent form of regulated necrosis. It is implicated in various human diseases, including ischemic organ damage and cancer. Here, we report the crucial role of autophagy, particularly autophagic degradation of cellular iron storage proteins (a process known as ferritinophagy), in ferroptosis. ...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2016.95
更新日期:2016-09-01 00:00:00
abstract::This article profiles current status of spread and control of HIV/AIDS in China. China has a significant population of MSM (men who have sex with men) and they have been becoming very much alive in many ways since 1990s due to recent social changes. Some surveys indicate that great many of MSM are engaged in high-risk...
journal_title:Cell research
pub_type: 杂志文章,评审
doi:10.1038/sj.cr.7290359
更新日期:2005-11-01 00:00:00
abstract::LRP16 was previously identified as an estrogen-induced gene in breast cancer cells. The responsiveness of LRP16 to estrogen and its functional effects in endometrial cancer (EC) cells are still unclear. Here, we show that the mRNA level and promoter activity of the LRP16 gene were significantly increased by 17beta-est...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2007.79
更新日期:2007-10-01 00:00:00
abstract::α-Tubulin detyrosination, largely catalyzed by vasohibins, is involved in many microtubule (MT)-related cellular events. In this study, we identified a core heterodimeric complex of human small vasohibin-binding protein (SVBP) and vasohibin 1 (VASH1) (hereafter denoted as SVBP-VASH1) that catalyzes the detyrosination ...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/s41422-019-0187-y
更新日期:2019-07-01 00:00:00
abstract::Plant reproduction is initiated by the specification of sporocytes that form haploid spores through meiosis. A new study in Arabidopsis published in Cell Research shows how the product of sporocyteless/nozzle, a key gene in this process, partners with co-repressors and transcription factors to promote spore formation,...
journal_title:Cell research
pub_type: 评论,杂志文章
doi:10.1038/cr.2014.164
更新日期:2015-01-01 00:00:00
abstract::Elimination of misfolded proteins of the endoplasmic reticulum (ER) requires their retrotranslocation from the ER to the cytosol via membrane-bound ubiquitin ligase complexes. Baldridge and Rapoport now reconstitute a key step of retrotranslocation, demonstrating a protein conduit gated by ubiquitination. ...
journal_title:Cell research
pub_type: 评论,杂志文章
doi:10.1038/cr.2016.92
更新日期:2016-10-01 00:00:00
abstract::Morphologically, caveolae and lipid rafts are two different membrane structures. They are often reported to share similar lipid and protein compositions, and are considered to be two subtypes of membrane lipid microdomains. By modifying sucrose density gradient flotation centrifugation, which is used to isolate lipid ...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2009.27
更新日期:2009-04-01 00:00:00
abstract::The endoplasmic reticulum (ER) is composed of the nuclear envelope, perinuclear sheets and a peripheral tubular network. The peripheral ER and mitochondria form tight contacts at specific subdomains, which coordinate the functions of the two organelles and are required for multiple cellular processes such as Ca2+ tran...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/s41422-018-0065-z
更新日期:2018-08-01 00:00:00
abstract::Craniofacial anomalies (CFAs) characterized by birth defects of skull and facial bones are the most frequent congenital disease. Genomic analysis has identified multiple genes responsible for CFAs; however, the underlying genetic mechanisms for the majority of CFAs remain largely unclear. Our previous study revealed t...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2016.51
更新日期:2016-06-01 00:00:00
abstract::Our previous study showed that hydroxyurea (Hu) could induce HEL cells to express human beta-globin gene. However the molecular mechanisms by which the expression of beta-globin gene is activated and regulated are poorly understood. Here we show that the binding patterns between the core DNA sequences (HS2 core sequen...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/sj.cr.7290144
更新日期:2002-12-01 00:00:00
abstract::Wor1 (white-opaque switching regulator 1) is a master regulator of the white-opaque switching in Candida albicans, an opportunistic human fungal pathogen, and is associated with its pathogenicity and commensality. Wor1 contains a conserved DNA-binding region at the N-terminus, consisting of two conserved segments (WOP...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2014.102
更新日期:2014-09-01 00:00:00
abstract::Differentiation of inflammatory macrophages from monocytes is characterized by an orderly integration of epigenetic and transcriptional regulatory mechanisms guided by lineage-determining transcription factors such as PU.1. Further activation of macrophages leads to a stimulus- or microenvironment-specific signal inte...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2016.1
更新日期:2016-02-01 00:00:00
abstract::Classical methyl-CpG binding proteins contain the conserved DNA binding motif methyl-cytosine binding domain (MBD), which preferentially binds to methylated CpG dinucleotides. These proteins serve as transcriptional repressors, mediating gene silencing via DNA cytosine methylation. Mutations in methyl-CpG binding prot...
journal_title:Cell research
pub_type: 杂志文章,评审
doi:10.1038/sj.cr.7290294
更新日期:2005-04-01 00:00:00
abstract::Dysregulation of beta-site APP-cleaving enzyme (BACE) and/or gamma-secretase leads to anomalous production of amyloid-beta peptide (Abeta) and contributes to the etiology of Alzheimer's disease (AD). Since these secretases mediate proteolytic processing of numerous proteins, little success has been achieved to treat A...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2010.3
更新日期:2010-02-01 00:00:00
abstract::Mechanical deformability is known to increase during cancer progression. Now Ma et al. identify a therapeutic strategy that targets deformable cells by microparticle-based drug delivery. ...
journal_title:Cell research
pub_type: 评论,杂志文章
doi:10.1038/cr.2016.54
更新日期:2016-06-01 00:00:00
abstract::Cytosine methylation of genomic DNA controls gene expression and maintains genome stability. How a specific DNA sequence is targeted for methylation by a methyltransferase is largely unknown. Here, we show that histone H3 tails lacking lysine 4 (K4) methylation function as an allosteric activator for methyltransferase...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2011.92
更新日期:2011-08-01 00:00:00
abstract::Autophagy is a conserved process that delivers cytosolic substances to the lysosome for degradation, but its direct role in the regulation of antiviral innate immunity remains poorly understood. Here, through high-throughput screening, we discovered that CCDC50 functions as a previously unknown autophagy receptor that...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/s41422-020-0362-1
更新日期:2021-01-01 00:00:00
abstract::Drosophila Wingless (Wg) acts as a morphogen during development. Wg secretion is controlled by a seven-pass transmembrane cargo Wntless (Wls). We have recently identified retromer as a key regulator involved in Wls trafficking. As sorting nexin (SNX) molecules are essential components of the retromer complex, we hypot...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2011.167
更新日期:2011-12-01 00:00:00
abstract::Heterotrimeric G proteins are known to function as messengers in numerous signal transduction pathways. The null mutation of RGA (rice heterotrimeric G protein alpha subunit), which encodes the alpha subunit of heterotrimeric G protein in rice, causes severe dwarfism and reduced responsiveness to gibberellic acid in r...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/sj.cr.7310111
更新日期:2006-12-01 00:00:00
abstract::Lineage conversion by expression of lineage-specific transcription factors is a process of epigenetic remodeling that has low efficiency. The mechanism by which a cell resists lineage conversion is largely unknown. Using hepatic-specific transcription factors Foxa3, Hnf1α and Gata4 (3TF) to induce hepatic conversion i...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2017.36
更新日期:2017-05-01 00:00:00
abstract::Polymerase I and transcript release factor (PTRF, also known as Cavin-1) is an essential component in the biogenesis and function of caveolae. Here, we show that PTRF expression is increased in senescent human fibroblasts. Importantly, overexpression of PTRF induced features characteristic of cellular senescence, wher...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2011.56
更新日期:2011-07-01 00:00:00
abstract::Several studies have demonstrated the clonal transmission of distinct differentiation and self-renewal properties in hematopoietic stem cells during the regeneration of blood production in transplanted recipients. A recent publication now identifies Vwf expression as a discriminating marker of a hematopoietic stem cel...
journal_title:Cell research
pub_type: 评论,杂志文章
doi:10.1038/cr.2013.139
更新日期:2014-01-01 00:00:00
abstract::Why stem cell numbers decline with age is a major question in regenerative biology and medicine. Skeletal muscle has emerged as a powerful paradigm to address this issue. Recently, genetic and cell marking strategies were used to uncover a new and causal relationship between muscle stem cells and differentiated fibers...
journal_title:Cell research
pub_type: 评论,杂志文章
doi:10.1038/cr.2013.3
更新日期:2013-04-01 00:00:00
abstract::While M1 macrophages are highly pro-inflammatory and microbicidal, M2 macrophages and the related tumor associated macrophages (TAMs) regulate tissue remodeling and angiogenesis and can display immunomodulatory activity. In July issue of Cell Research, Zhang et al. show that ROS production, critical for the activation...
journal_title:Cell research
pub_type: 评论,杂志文章
doi:10.1038/cr.2013.88
更新日期:2013-08-01 00:00:00
abstract::A monoclonal antibody, LC-1, recognizing lung cancer associated common antigens was obtained in authors' laboratory. Its single chain Fv fragment (ScFv) named LC-1 ScFv was constructed based on recombinant phage displayed techniques. For expression on cell membrane, LC-1 ScFv was cloned into pDisplay vector, which dir...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/sj.cr.7290109
更新日期:2002-03-01 00:00:00
abstract::T helper (TH)-cell subsets, such as TH1 and TH17, mediate inflammation in both peripheral tissues and central nervous system. Here we show that STAT5 is required for T helper-cell pathogenicity in autoimmune neuroinflammation but not in experimental colitis. Although STAT5 promotes regulatory T cell generation and imm...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2014.154
更新日期:2014-12-01 00:00:00
abstract::Enucleation of erythroblasts during terminal differentiation is unique to mammals. Although erythroid enucleation has been extensively studied, only a few genes, including retinoblastoma protein (Rb), have been identified to regulate nuclear extrusion. It remains largely undefined by which signaling molecules, the ext...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2011.159
更新日期:2012-03-01 00:00:00
abstract::Polyamines are implicated in regulating various developmental processes in plants, but their exact roles and how they govern these processes still remain elusive. We report here an Arabidopsis bushy and dwarf mutant, bud2, which results from the complete deletion of one member of the small gene family that encodes S-a...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/sj.cr.7310056
更新日期:2006-05-01 00:00:00
abstract::A wide variety of neurons and glial cells differentiate from common precursor cells in the developing nervous system. During this process, Notch-mediated cell-cell interaction is essential for maintenance of dividing cells and subsequent generation of cell type diversity. Activation of Notch inhibits cellular differen...
journal_title:Cell research
pub_type: 杂志文章,评审
doi:10.1038/sj.cr.7290016
更新日期:1999-09-01 00:00:00
abstract::The nucleotide sequence deduced from the amino acid sequence of the scorpion insectotoxin AaIT was chemically synthesized and was expressed in Escherichia coli. The authenticity of this in vitro expressed peptide was confirmed by N-terminal peptide sequencing. Two groups of bioassays, artificial diet incorporation ass...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/sj.cr.7290120
更新日期:2002-06-01 00:00:00