Abstract:
:Our previous study showed that hydroxyurea (Hu) could induce HEL cells to express human beta-globin gene. However the molecular mechanisms by which the expression of beta-globin gene is activated and regulated are poorly understood. Here we show that the binding patterns between the core DNA sequences (HS2 core sequence -10681 approximately -10971 bp, HS3 core sequence -14991 approximately -14716 bp and HS4 core sequence -18586 approximately -18306 bp) of DNase I hypersensitive sites in the human beta-globin LCR and nuclear matrix proteins isolated from Hu induced and uninduced HEL cells are quite different. Results demonstrated that nuclear matrix proteins might play important roles in regulating the expression of human beta-like globin genes through their interaction with HSs (HS2, HS3 and HS4 core sequences) in the LCR. Moreover, the results obtained from the in vitro DNA-matrix binding assay showed that the core DNA sequences of DNase I hypersensitive sites (HS2, HS3 and HS4) were unable to bind to the nuclear matrix isolated from uninduced HEL cells; in addition, HS2 core DNA sequence was capable of binding to the nuclear matrix prepared from Hu-induced HEL cells, while both HS3 and HS4 core DNA sequences could not do so. Results indicated that the HS2 core DNA sequence may be a functional MAR (matrix attachment region). We suggest that the HS2 core DNA sequence binding to the nuclear matrix in Hu-induced HEL cells may open the structure of chromatin to make the LCR accessible to the promoter of beta-globin gene and to promote its transcription.
journal_name
Cell Resjournal_title
Cell researchauthors
Zhang SB,Qian RLdoi
10.1038/sj.cr.7290144keywords:
subject
Has Abstractpub_date
2002-12-01 00:00:00pages
411-6issue
5-6eissn
1001-0602issn
1748-7838journal_volume
12pub_type
杂志文章相关文献
CELL RESEARCH文献大全abstract::The cytotoxic T lymphocyte antigen-4 (CTLA-4)-blocking antibody ipilimumab induces immune-mediated long-term control of metastatic melanoma in a fraction of patients. Although ipilimumab undoubtedly exerts its therapeutic effects via immunostimulation, thus far clinically useful, immunologically relevant biomarkers th...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2015.3
更新日期:2015-02-01 00:00:00
abstract::Protein ubiquitination by E3 ubiquitin ligases plays an important role in cancer development. In this study, we provide experimental evidence that a RING-finger-containing protein RNF13 is an ER/Golgi membrane-associated E3 ubiquitin ligase and its RING finger domain is required for the ubiquitin ligase activity. Immu...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2008.285
更新日期:2009-03-01 00:00:00
abstract::Bone-marrow-derived mesenchymal stem cells (MSCs) have been shown to possess immunosuppressive properties, e.g., by inhibiting T cell proliferation. Activated T cells can also enhance the immunosuppression ability of MSCs. The precise mechanisms underlying MSC-mediated immunosuppression remain largely undefined, altho...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2008.80
更新日期:2008-08-01 00:00:00
abstract::Morphologically, caveolae and lipid rafts are two different membrane structures. They are often reported to share similar lipid and protein compositions, and are considered to be two subtypes of membrane lipid microdomains. By modifying sucrose density gradient flotation centrifugation, which is used to isolate lipid ...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2009.27
更新日期:2009-04-01 00:00:00
abstract::A paracrine regulation was recently proposed in human embryonic stem cells (hESCs) grown in mouse embryonic fibroblast (MEF)-conditioned media (MEF-CM), where hESCs spontaneously differentiate into autologous fibroblast-like cells to maintain culture homeostasis by producing TGF-beta and insulin-like growth factor-II ...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2009.35
更新日期:2009-06-01 00:00:00
abstract::The term epigenetics refers to heritable changes not encoded by DNA. The organization of DNA into chromatin fibers affects gene expression in a heritable manner and is therefore one mechanism of epigenetic inheritance. Large parts of eukaryotic genomes consist of constitutively highly condensed heterochromatin, import...
journal_title:Cell research
pub_type: 杂志文章,评审
doi:10.1038/cr.2009.13
更新日期:2009-03-01 00:00:00
abstract::Various factors/pathways including hormonal regulation have been suggested to control herpes simplex virus type 1 (HSV-1) latency and reactivation. Our computer analysis identified a DNA repeat containing thyroid hormone-responsive elements (TRE) in the regulatory region of HSV-1 latency-associated transcript (LAT). T...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2010.50
更新日期:2010-05-01 00:00:00
abstract::Interleukin (IL)-8 is a potent neutrophil chemotactic factor and a crucial mediator in neutrophil-dependent inflammation. Various cell types produce IL-8, either in response to external stimuli such as cytokines or bacterial infection, or after malignant transformation. Anti-IL-8 strategies have been considered for an...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/sj.cr.7290275
更新日期:2005-02-01 00:00:00
abstract::The authors apologized for the spelling mistake of author name for Zhengyu Ju. The correct form is Zhenyu Ju. We apologize for any hassle that may have been caused by our mistake. ...
journal_title:Cell research
pub_type: 杂志文章,已发布勘误
doi:10.1038/s41422-018-0063-1
更新日期:2018-08-01 00:00:00
abstract::Over the past four years, the field of the innate immune response has been highly influenced by the discovery of the IkappaB kinase (IKK)-related kinases, TANK Binding Kinase 1 (TBK1) and IKKi, which regulate the activity of interferon regulatory factor (IRF)-3/IRF-7 and NF-kappaB transcription factors. More recently,...
journal_title:Cell research
pub_type: 杂志文章,评审
doi:10.1038/cr.2008.273
更新日期:2008-09-01 00:00:00
abstract::In 2006, the "wall came down" that limited the experimental conversion of differentiated cells into the pluripotent state. In a landmark report, Shinya Yamanaka's group described that a handful of transcription factors (Oct4, Sox2, Klf4 and c-Myc) can convert a differentiated cell back to pluripotency over the course ...
journal_title:Cell research
pub_type: 杂志文章,评审
doi:10.1038/cr.2011.28
更新日期:2011-03-01 00:00:00
abstract::Oct4 is a well-known transcription factor that plays fundamental roles in stem cell self-renewal, pluripotency, and somatic cell reprogramming. However, limited information is available on Oct4-associated protein complexes and their intrinsic protein-protein interactions that dictate Oct4's critical regulatory activit...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2011.179
更新日期:2012-01-01 00:00:00
abstract::Type I interferon (IFN) production plays pivotal roles in host antiviral innate immune responses, but an excessive production of type I IFN leads to the development of immunopathological conditions. Investigations on the regulatory mechanisms underlying host type I IFN production are currently of great interest. Here,...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2015.108
更新日期:2015-10-01 00:00:00
abstract::Transforming growth factor-beta utilizes a multitude of intracellular signaling pathways in addition to Smads to regulate a wide array of cellular functions. These non-canonical, non-Smad pathways are activated directly by ligand-occupied receptors to reinforce, attenuate, or otherwise modulate downstream cellular res...
journal_title:Cell research
pub_type: 杂志文章,评审
doi:10.1038/cr.2008.328
更新日期:2009-01-01 00:00:00
abstract::Since its discovery in 1989, researchers strive after a small animal model for Hepatitis C virus infection, so far with very limited success. A study recently published in Nature now for the first time reports the recapitulation of the complete life cycle of this virus in inbred mice with a functional adaptive immune ...
journal_title:Cell research
pub_type: 评论,杂志文章
doi:10.1038/cr.2013.132
更新日期:2013-12-01 00:00:00
abstract::Endothelial progenitor cells (EPCs) participate in the processes of postnatal neovascularization and re-endothelialization in response to tissue ischemia and endothelial injury. The level of EPCs present has been found to be directly associated with the outcome of cardiovascular diseases, and could be regulated by sti...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2008.69
更新日期:2008-07-01 00:00:00
abstract::Calcium-dependent proteolytic calpains are implicated in a variety of physiological processes, as well as pathologies associated with calcium overload. However, the mechanism by which calpain is activated remains elusive since intracellular calcium levels under physiological conditions do not reach the high concentrat...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/s41422-020-0388-4
更新日期:2020-08-26 00:00:00
abstract::Heterotrimeric G proteins are known to function as messengers in numerous signal transduction pathways. The null mutation of RGA (rice heterotrimeric G protein alpha subunit), which encodes the alpha subunit of heterotrimeric G protein in rice, causes severe dwarfism and reduced responsiveness to gibberellic acid in r...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/sj.cr.7310111
更新日期:2006-12-01 00:00:00
abstract::Influenza A viruses (IAVs), particularly H1N1, H5N1 and H7N9, pose a substantial threat to public health worldwide. Here, we report that MIR2911, a honeysuckle (HS)-encoded atypical microRNA, directly targets IAVs with a broad spectrum. MIR2911 is highly stable in HS decoction, and continuous drinking or gavage feedin...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2014.130
更新日期:2015-01-01 00:00:00
abstract::Circular RNAs (circRNAs) were only recently discovered as a new class of noncoding RNAs, functionally still largely uncharacterized. Three publications that appeared concurrently in Cell Research and Molecular Cell provide initial evidence for certain endogenous circRNAs coding for proteins. ...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2017.70
更新日期:2017-06-01 00:00:00
abstract::In the present article, we report that DR4 or DR5 overexpression dramatically activates the release of the inflammatory cytokines IL-8, TNF-alpha, CCL20, MIP-2 and MIP-1beta in an NF-kappaB-dependent manner in 293T, MDA-MB-231 and HCT-116 cells. We showed that death receptor-mediated signals were extracellular domain-...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2009.57
更新日期:2009-06-01 00:00:00
abstract::Mouse cloning from fertilized eggs can assist development of approaches for the production of "genetically tailored" human embryonic stem (ES) cell lines that are not constrained by the limitations of oocyte availability. However, to date only zygotes have been successfully used as recipients of nuclei from terminally...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2010.180
更新日期:2011-05-01 00:00:00
abstract::There is strong relationship between melanocortin-1 receptor (MC1R) gene variants and human hair color and skin type. Based on a sequencing study of MC1R gene in 50 individuals from the Uygur, Tibetan, Wa and Dai ethnic populations, we discuss the occurrence of 7 mc1r variants consisting of 5 nonsynonymous sites (Val6...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/sj.cr.7290070
更新日期:2001-03-01 00:00:00
abstract::A potential link between inflammation and cancer has been suspected for over a century, but the exact molecular mechanisms connecting the two remained nebulous. We proposed that NF-kappaB transcription factors regulated via the IkappaB kinase (IKK) complex play a critical role in coupling inflammation and cancer and h...
journal_title:Cell research
pub_type: 杂志文章,评审
doi:10.1038/cr.2008.30
更新日期:2008-03-01 00:00:00
abstract::The neurodegenerative polyglutamine diseases are caused by an expansion of unstable polyglutamine repeats in various disease proteins. Although these mutant proteins are expressed ubiquitously in neuronal and non-neuronal cells, they cause selective degeneration of specific neuronal populations. Recently, increasing e...
journal_title:Cell research
pub_type: 杂志文章,评审
doi:10.1038/cr.2010.32
更新日期:2010-04-01 00:00:00
abstract::Phosphatidylinositol 3-phosphate (PI3P) plays essential roles in vesicular trafficking, organelle biogenesis and autophagy. Two class III phosphatidylinositol 3-kinase (PI3KC3) complexes have been identified in mammals, the ATG14L complex (PI3KC3-C1) and the UVRAG complex (PI3KC3-C2). PI3KC3-C1 is crucial for autophag...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2017.94
更新日期:2017-08-01 00:00:00
abstract::Pluripotent stem cells (PSCs) harbor constitutive DNA replication stress during their rapid proliferation and the consequent genome instability hampers their applications in regenerative medicine. It is therefore important to understand the regulatory mechanisms of replication stress response in PSCs. Here, we report ...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2017.139
更新日期:2018-01-01 00:00:00
abstract::Mouse sp56 is considered as one of the candidates for mouse zona pellucida 3 (mZP3) receptor. Up to date, its homologue has only been cloned from guinea pig, namely AM67. Based on the cDNA sequence of mouse sp56, we designed a pair of primer to amplify its homologue from rat testis cDNA. Using RT-PCR, two fragments of...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/sj.cr.7290156
更新日期:2003-04-01 00:00:00
abstract::In mammals, the inheritance of mitochondrion and its DNA (mtDNA) is strictly maternal, despite the fact that a sperm can inject up to 100 functional mitochondria into the oocyte during fertilization. The mechanisms responsible for the elimination of the paternal mitochondria remain largely unknown. We report here that...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2011.182
更新日期:2011-12-01 00:00:00
abstract::Type I interferon (IFN-I) production is efficiently induced to ensure a potent innate immune response to viral infection. How this response can be enhanced, however, remains to be explored. Here, we identify a new cytoplasmic long non-coding RNA (lncRNA), lncLrrc55-AS, that drives a positive feedback loop to promote i...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/s41422-019-0193-0
更新日期:2019-08-01 00:00:00