Abstract：
:AS160 has emerged as a key player in insulin-mediated glucose transport through controlling GLUT4 trafficking, which is thought to be regulated by insulin-stimulated phosphorylation of sites including the 14-3-3 binding phospho-Thr649 (equivalent to Thr642 in human AS160). To define physiological roles of AS160-Thr649 phosphorylation and 14-3-3 binding in glucose homeostasis, we substituted this residue by a nonphosphorylatable alanine by knockin mutation in mice. The mutant protein was expressed at normal levels, while insulin-stimulated AS160 binding to 14-3-3s was abolished in homozygous knockin mice. These animals displayed impaired glucose disposal and insulin sensitivity, which were associated with decreased glucose uptake in vivo. Insulin-stimulated glucose transport and cell surface GLUT4 content were reduced in isolated muscles, but not in adipocytes. These results provide genetic evidence that insulin-induced AS160-Thr649 phosphorylation and/or its binding to 14-3-3 play an important role in regulating whole-body glucose homeostasis, at least in part through regulating GLUT4 trafficking in muscle.
journal_name
Cell Metabjournal_title
Cell metabolismauthors
Chen S,Wasserman DH,MacKintosh C,Sakamoto Kdoi
10.1016/j.cmet.2010.12.005subject
Has Abstractpub_date
2011-01-05 00:00:00pages
68-79issue
1eissn
1550-4131issn
1932-7420pii
S1550-4131(10)00447-Xjournal_volume
13pub_type
杂志文章相关文献
Cell Metabolism文献大全abstract：:Cannabinoid signaling by CB1 receptors drives fibrogenesis and fat accumulation in liver. A report in this issue of Cell Metabolism (Jeong et al., 2008) now links hepatic stellate cells, a resident liver fibrogenic cell type, to the generation of steatosis through production of the endocannabinoid 2-arachidonoylglycer...
journal_title：Cell metabolism
pub_type： 评论,杂志文章
doi：10.1016/j.cmet.2008.02.002
更新日期：2008-03-01 00:00:00
abstract：:TRPM channels have emerged as key mediators of diverse physiological functions. However, the ionic permeability relevant to physiological function in vivo remains unclear for most members. We report that the single Drosophila TRPM gene (dTRPM) generates a conductance permeable to divalent cations, especially Zn(2+) an...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2010.08.012
更新日期：2010-10-06 00:00:00
abstract：:Over the past years, plenty of evidence has emerged illustrating how metabolism supports many aspects of cellular function and how metabolic reprogramming can drive cell differentiation and fate. Here, we present a method to assess the metabolic configuration of single cells within their native tissue microenvironment...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2017.08.014
更新日期：2017-11-07 00:00:00
abstract：:Hormesis is an adaptive stress response implicated in longevity regulation. Schroeder et al. (2013) have now connected stress, epigenetic changes, and aging in yeast by showing that mitochondria-derived reactive oxygen species modulate the chromatin binding capacity of the histone demethylase Rph1p at subtelomeres, re...
journal_title：Cell metabolism
pub_type： 评论,杂志文章
doi：10.1016/j.cmet.2013.05.015
更新日期：2013-06-04 00:00:00
abstract：:Proper control of hepatic glucose production is central to whole-body glucose homeostasis, and its disruption plays a major role in diabetes. Here, we demonstrate that although established as an intracellular lipid chaperone, aP2 is in fact actively secreted from adipocytes to control liver glucose metabolism. Secreti...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2013.04.012
更新日期：2013-05-07 00:00:00
abstract：:The fibroblast growth factor receptor 4 (FGFR4)-R388 single-nucleotide polymorphism has been associated with cancer risk and prognosis. Here we show that the FGFR4-R388 allele yields a receptor variant that preferentially promotes STAT3/5 signaling. This STAT activation transcriptionally induces Grb14 in pancreatic en...
journal_title：Cell metabolism
pub_type： 杂志文章,收录出版
doi：10.1016/j.cmet.2013.05.002
更新日期：2013-06-04 00:00:00
abstract：:The "Rosies" of Cell Metabolism persist as a new generation enters the stage. With inspiration from this issue's cover art, we celebrate young and diverse scientists and the mentorship that has guided them throughout. Their stories come from different corners of the world but are tied together by a common thread of te...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2017.08.021
更新日期：2017-09-05 00:00:00
abstract：:Reduced reproduction is associated with increased fat storage and prolonged life span in multiple organisms, but the underlying regulatory mechanisms remain poorly understood. Recent studies in several species provide evidence that reproduction, fat metabolism, and longevity are directly coupled. For instance, germlin...
journal_title：Cell metabolism
pub_type： 杂志文章,评审
doi：10.1016/j.cmet.2012.12.003
更新日期：2013-01-08 00:00:00
abstract：:miRNAs can be found in serum and other body fluids and serve as biomarkers for disease. More importantly, secreted miRNAs, especially those in extracellular vesicles (EVs) such as exosomes, may mediate paracrine and endocrine communication between different tissues and thus modulate gene expression and the function of...
journal_title：Cell metabolism
pub_type： 杂志文章,评审
doi：10.1016/j.cmet.2019.07.011
更新日期：2019-10-01 00:00:00
abstract：:Oxidants are thought to damage cells, and stem cells are viewed as particularly vulnerable to oxidative stress. Now, a new study (Morimoto et al., 2013) suggests that the self-renewal of certain stem cells may actually require reactive oxygen species (ROS). ...
journal_title：Cell metabolism
pub_type： 评论,杂志文章
doi：10.1016/j.cmet.2013.06.007
更新日期：2013-07-02 00:00:00
abstract：:Adipocyte mobilization of fatty acids (lipolysis) is instrumental for energy expenditure. Lipolysis displays both spontaneous (basal) and hormone-stimulated activity. It is unknown if lipolysis is important for future body weight gain and associated disturbed glucose metabolism, and this was presently investigated in ...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2018.05.004
更新日期：2018-07-03 00:00:00
abstract：:Mammalian cells detect decreases in oxygen concentrations to activate a variety of responses that help cells adapt to low oxygen levels (hypoxia). One such response is stabilization of the protein HIF-1 alpha, a component of the transcription factor HIF-1. Here we show that a small interfering RNA (siRNA) against the ...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2005.05.002
更新日期：2005-06-01 00:00:00
abstract：:Individuals with elevated LDL-cholesterol levels have an increased risk for cardiovascular disease. Despite lipid lowering strategies, however, a significant cardiovascular risk remains. Bekkering et al. show that monocytes from patients with familial hypercholesterolemia have a trained immunity phenotype and that lip...
journal_title：Cell metabolism
pub_type： 信件
doi：10.1016/j.cmet.2019.05.014
更新日期：2019-07-02 00:00:00
abstract：:Diurnal oscillations of gene expression controlled by the circadian clock and its connected feeding rhythm enable organisms to coordinate their physiologies with daily environmental cycles. While available techniques yielded crucial insights into regulation at the transcriptional level, much less is known about tempor...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2016.10.003
更新日期：2017-01-10 00:00:00
abstract：:A BMI in the normal range associates with a decreased risk of cardiometabolic disease and all-cause mortality. However, not all subjects in this BMI range have this low risk. Compared to people who are of normal weight and metabolically healthy, subjects who are of normal weight but metabolically unhealthy (∼20% of th...
journal_title：Cell metabolism
pub_type： 杂志文章,评审
doi：10.1016/j.cmet.2017.07.008
更新日期：2017-08-01 00:00:00
abstract：:Because MYC plays a causal role in many human cancers, including those with hypoxic and nutrient-poor tumor microenvironments, we have determined the metabolic responses of a MYC-inducible human Burkitt lymphoma model P493 cell line to aerobic and hypoxic conditions, and to glucose deprivation, using stable isotope-re...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2011.12.009
更新日期：2012-01-04 00:00:00
abstract：:Cells within the islet of Langerhans are heterogeneous. Camunas-Soler et al. (2020) implement a patch-seq technique to collect both transcriptomic and electrophysiological data from the same cell. By doing so, they discover new genes that correlate with functional heterogeneity and find that shifts in these correlatio...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2020.04.012
更新日期：2020-05-05 00:00:00
abstract：:The mechanistic target of rapamycin complex 1 (mTORC1) coordinates nutrient availability with cell growth. Recent reports by Sabatini and coworkers (Saxton et al., 2016; Wolfson et al., 2016) characterize a cytoplasmic amino acid receptor that couples the binding of leucine to the activation of mTORC1. ...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2016.02.012
更新日期：2016-03-08 00:00:00
abstract：:Current evidence suggests that hypothalamic fatty acid metabolism may play a role in regulating food intake; however, confirmation that it is a physiologically relevant regulatory system of feeding is still incomplete. Here, we use pharmacological and genetic approaches to demonstrate that the physiological orexigenic...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2008.03.006
更新日期：2008-05-01 00:00:00
abstract：:Repression of mitochondrial respiration represents an evolutionarily ancient cellular adaptation to hypoxia and profoundly influences cell survival and function; however, the underlying molecular mechanisms are incompletely understood. Primarily utilizing pulmonary arterial endothelial cells as a representative hypoxi...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2009.08.015
更新日期：2009-10-01 00:00:00
abstract：:Defects in insulin secretion play a central role in the pathogenesis of type 2 diabetes, yet the mechanisms driving beta-cell dysfunction remain poorly understood, and therapies to preserve glucose-dependent insulin release are inadequate. We report a luminescent insulin secretion assay that enables large-scale invest...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2014.12.010
更新日期：2015-01-06 00:00:00
abstract：:Serotonin (5-HT) and leptin play important roles in the modulation of energy balance. Here we investigated mechanisms by which leptin might interact with CNS 5-HT pathways to influence appetite. Although some leptin receptor (LepRb) neurons lie close to 5-HT neurons in the dorsal raphe (DR), 5-HT neurons do not expres...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2011.03.016
更新日期：2011-05-04 00:00:00
abstract：:The final steps in the production of adenosine triphosphate (ATP) in mitochondria are executed by a series of multisubunit complexes and electron carriers, which together constitute the oxidative phosphorylation (OXPHOS) system. OXPHOS is under dual genetic control, with communication between the nuclear and mitochond...
journal_title：Cell metabolism
pub_type： 杂志文章,评审
doi：10.1016/j.cmet.2005.12.001
更新日期：2006-01-01 00:00:00
abstract：:Skin aging is of considerable interest from various perspectives, ranging from aesthetics to cancer development. Zhang et al. (2019) elucidate an immunological consequence of aging in the adipose layer of skin. Age-dependent increases in TGF-β signaling impair fibroblast adipogenic potential that results in impaired a...
journal_title：Cell metabolism
pub_type： 评论,杂志文章
doi：10.1016/j.cmet.2019.02.006
更新日期：2019-03-05 00:00:00
abstract：:Cutting down calories prolongs life, but how this works remains largely unknown. A recent study in Nature (Mair et al., 2011) shows that life span extension triggered by the energy-sensing protein kinase AMPK is mediated by an evolutionarily conserved transcriptional circuit involving CRTC-1 and CREB. ...
journal_title：Cell metabolism
pub_type： 评论,杂志文章
doi：10.1016/j.cmet.2011.03.012
更新日期：2011-04-06 00:00:00
abstract：:XBP1 is a key regulator of the unfolded protein response (UPR), which is involved in a wide range of physiological and pathological processes. XBP1 ablation in liver causes profound hypolipidemia in mice, highlighting its critical role in lipid metabolism. XBP1 deficiency triggers feedback activation of its upstream e...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2012.09.004
更新日期：2012-10-03 00:00:00
abstract：:Immune cell function and fate are intimately linked to engagement of metabolic pathways. The contribution of core metabolic pathways to immune cell bioenergetics has been vigorously investigated in recent years. However, precisely how other peripheral metabolic pathways support immune cells beyond energy generation is...
journal_title：Cell metabolism
pub_type： 杂志文章,评审
doi：10.1016/j.cmet.2017.06.019
更新日期：2017-07-05 00:00:00
abstract：:Nuclear receptors (NRs) are key regulators of gene expression and physiology. Nearly half of all human NRs lack endogenous ligands including estrogen-related receptor α (ERRα). ERRα has important roles in cancer, metabolism, and skeletal homeostasis. Affinity chromatography of tissue lipidomes with the ERRα ligand-bin...
journal_title：Cell metabolism
pub_type： 杂志文章
doi：10.1016/j.cmet.2015.12.010
更新日期：2016-03-08 00:00:00
abstract：:Obesity-related morbidity and mortality are related to fat accumulation and fat distribution in humans. Two large-scale meta-analyses recently published in Nature by Shungin et al. (2015) and Locke et al. (2015) report novel genetic associations for central and overall obesity; these greatly advance our understanding ...
journal_title：Cell metabolism
pub_type： 评论,杂志文章
doi：10.1016/j.cmet.2015.03.016
更新日期：2015-04-07 00:00:00
abstract：:Reporting in Cell Metabolism, Pflimlin et al. recently found no beneficial effect of PAHSAs on glucose control in mice on several high-fat diets. Kuda cautions that due to methodological differences, the data must be carefully reinterpreted, emphasizing that olive oil contains high amounts of FAHFAs, potentially maski...
journal_title：Cell metabolism
pub_type： 评论,信件
doi：10.1016/j.cmet.2018.09.006
更新日期：2018-10-02 00:00:00
