Abstract:
INTRODUCTION:The alterations in dentin tissue depending on increasing age might cause different adhesion capability of bacteria, yielding differences in clinical approaches regarding root canal irrigation. This study, therefore, aimed to evaluate the effects of ethylenediaminetetraacetic acid (EDTA) and sodium hypochlorite (NaOCl) on Enterococcus faecalis biofilm growth in root canal dentin of young and old individuals. METHODS:The root canals of extracted young (<30 years) and old (>60 years) single-rooted human teeth were sectioned at the crown and the apical parts. The root canals of the mid-root sections were enlarged with #2 Gates-Glidden burs. After treatment with 17% EDTA + 2.5% NaOCl, 17% EDTA alone, 2.5% NaOCl alone, or saline, the samples were incubated in E. faecalis suspension for 24 hours. Thereafter, root canal samples were enlarged again with #3 Gates-Glidden burs, and the removed dentin chips were collected. Bacteria were dispersed by using sonication, serially diluted, and then plated for counting on agar plates as colony-forming units. Scanning electron microscopy and confocal laser scanning microscopy investigations were also carried out to examine the biofilm formation on the dentin. Data were analyzed with Kruskal-Wallis test and Mann-Whitney U test with Bonferroni adjustment. RESULTS:Combination of EDTA and NaOCl significantly reduced the amount of intracanal biofilm in both age groups (P < .01). However, the bacterial counts of E. faecalis in the old group were still higher (P < .05). CONCLUSIONS:It might be suggested that root canals from elderly population are more susceptible to canal infection. However, combined application of EDTA and NaOCl significantly reduces the amount of intracanal biofilm.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Ozdemir HO,Buzoglu HD,Calt S,Stabholz A,Steinberg Ddoi
10.1016/j.joen.2010.01.008subject
Has Abstractpub_date
2010-05-01 00:00:00pages
842-6issue
5eissn
0099-2399issn
1878-3554pii
S0099-2399(10)00045-2journal_volume
36pub_type
杂志文章abstract::The purpose of this in vitro study was to compare dye leakage between canals backfilled in a single increment and canals backfilled in multiple increments using the Obtura II system with two different sealers. Sixty extracted single canal teeth were decoronated, cleaned, and shaped. After master cone fit and placement...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(99)80320-3
更新日期:1999-09-01 00:00:00
abstract:INTRODUCTION:It has been widely accepted that dental pulp stem cells (DPSCs), which are a class of self-renewal and differentiation potential of adult stem cells, play an important role in the repair procession of pulp's inflammation. We investigated whether tumor necrosis factor alpha (TNF-α) could induce the prolifer...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2015.02.020
更新日期:2015-07-01 00:00:00
abstract:INTRODUCTION:In carious teeth, transforming growth factor beta 1 (TGF-β1) is released from the dentin matrix and possibly activated in an acidic environment. Conversely, EDTA solutions with a neutral to slightly alkaline pH are used in clinics to promote cell homing in regenerative endodontic procedures. We hypothesize...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2018.09.015
更新日期:2019-01-01 00:00:00
abstract::In this study, we examined the effects of TNF-alpha on Bone morphogenetic protein (BMP-2), Smads (which play intracellular signaling of BMPs) expression and alkaline phosphatase (ALP) activity of human dental pulp (HDP) cells to clarify the mechanism of tertiary dentin formation. The quantity of RT-PCR product for BMP...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2005.12.007
更新日期:2006-06-01 00:00:00
abstract:INTRODUCTION:This study compared the efficacy of a reciprocating single-instrument system and a rotary multi-instrument system followed by a supplementary approach with a finishing instrument in removing the filling material from curved canals during retreatment. METHODS:Forty mesial canals from extracted mandibular m...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2016.04.007
更新日期:2016-07-01 00:00:00
abstract::When dentin is planed by endodontic instruments, a smear layer forms. Whether this layer should be removed is unknown and controversial. This study was conducted to assess the effect of the smear layer on retention of bacteria using an in vitro root canal bacterial colonization model. Canals of 26 extracted human cani...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(06)81186-6
更新日期:1994-02-01 00:00:00
abstract:INTRODUCTION:A ferrule on anterior endodontic-treated teeth has been evaluated using clinical trials, in vitro tests, and finite element analysis (FEA). The patient-specific FEA with a nonuniform ferrule and nonlinear contact biting load associated with clinical validation can be used to predict failure. METHODS:A pat...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2017.04.024
更新日期:2017-09-01 00:00:00
abstract:INTRODUCTION:Mineral trioxide aggregate (MTA) and calcium silicate (CS) cements exhibit acceptable levels of cytocompatibility. The aim of the present study was to evaluate MTA and CS cement genotoxicity in a human osteosarcoma cell line (MG63). METHODS:A mitochondrial colorimetric assay was used to evaluate the MG63 ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2010.03.039
更新日期:2010-07-01 00:00:00
abstract::Endodontic therapy has played an important role in maintaining the integrity of the natural dentition as a fully functional and esthetic masticatory apparatus. Although the sealing of the root canal system is usually accomplished by the conservative endodontic approach, cases which have failed or which involve perfora...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(06)81793-0
更新日期:1991-10-01 00:00:00
abstract::The purpose of this study was to compare clinical, radiographic, and histopathologic effects of Nd:YAG laser pulpotomy to formocresol pulpotomy on human primary teeth. Patients with at least two vital primary molar teeth that required pulpotomy, because of pulpal exposure to caries, were selected for this study. After...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2006.12.013
更新日期:2007-04-01 00:00:00
abstract:INTRODUCTION:The occurrence of multiple injuries of the same tooth is extremely rare. This case report describes the treatment in a midroot and complicated crown-root-fractured permanent incisor that remained untreated for 4 years after the accident. METHODS:The tooth was treated by delayed partial pulpotomy using cal...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2010.03.036
更新日期:2010-07-01 00:00:00
abstract:INTRODUCTION:The lysyl oxidase (LOX) family is an emerging family of amine oxidases responsible for the formation of collagen fibrils in the extracellular matrix. To date, 5 LOX family genes have been identified in humans, encoding LOX and LOX-like proteins (LOXL, LOXL2, LOXL3, and LOXL4). The goal of this study was to...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2012.03.014
更新日期:2012-06-01 00:00:00
abstract:INTRODUCTION:Currently, the success of periapical microsurgery is determined by the restoration of the lamina dura and the elimination of symptoms. However, inadequate site preservation may prevent later implant placement. Although not possible before, the advent of cone-beam computed tomographic imaging and computer-a...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2018.11.002
更新日期:2019-04-01 00:00:00
abstract:INTRODUCTION:Squamous odontogenic tumor-like epithelial islands occurring in the walls of odontogenic cysts are histologically identical to the squamous odontogenic tumor (SOT). Microscopically, the squamous odontogenic tumor-like proliferations (SOTLPs) share certain histologic features with SOT, acanthomatous and des...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2011.02.010
更新日期:2011-05-01 00:00:00
abstract::The aim of this study was to determine the effects of patient's age on the prevalence and depth of bacterial penetration inside dentinal tubules. Fifty-six single-rooted teeth were divided in two groups based on the patient's age: young (ages 18-25) and old (age >or=60). Teeth were instrumented and inoculated with a s...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2008.10.004
更新日期:2009-01-01 00:00:00
abstract:INTRODUCTION:In regenerative endodontic treatment (RET) for immature permanent tooth, better treatment results could be obtained by applying platelet-rich plasma (PRP) as the scaffold rather than the blood clot. The goal of this study was to compare the histologic differences between using PRP and blood clot in RET. M...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2014.03.020
更新日期:2014-09-01 00:00:00
abstract::This case report describes the clinical, radiologic, and histopathologic features of the glandular odontogenic cyst (GOC). Although a relatively rare lesion not previously reported in the endodontic literature, the GOC is recommended for inclusion in a differential diagnosis of a dentoalveolar radiolucency. The GOC is...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200403000-00014
更新日期:2004-03-01 00:00:00
abstract::Studies of endodontic outcomes suggest that fracture of the tooth after endodontic therapy may be a greater problem than endodontic reinfection. Immediate full coverage with or without a post and core is the best way to prevent fracture. Unfortunately, in many population areas, due predominantly to cost, this restorat...
journal_title:Journal of endodontics
pub_type: 杂志文章,评审
doi:10.1016/j.joen.2019.12.012
更新日期:2020-04-01 00:00:00
abstract::The cytotoxicity against two mesenchymal cell lines, L-929 and WI-38, and two epithelial cell lines, KB and HeLa S-3, of lipopolysaccharide (LPS) purified from strains of Bacteroides gingivalis, Fusobacterium nucleatum, and Veillonella parvula isolated from infected root canals was investigated. The inhibition of cell...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(89)80195-5
更新日期:1989-11-01 00:00:00
abstract::Thirteen teeth in human cadaver sections were accessed and #15 K-files were placed at various lengths in one root canal of each tooth. Using a positioner to ensure duplicate views, radiographs were taken with both Ultraspeed and Ektaspeed Plus X-ray film. Each of the 26 radiographs was coded and then placed in random ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(98)80216-1
更新日期:1998-01-01 00:00:00
abstract::The aims of this study were to compare Resilon (Resilon Research LLC, Madison, CT) in conjunction with either a bonding (Epiphany; Pentron Clinical Technologies, Wallingford, CT) or a nonbonding endodontic sealer (Pulp Canal Sealer; Kerr Corporation, Orange, CA) to EndoRez (Ultradent Products Inc, South Jordan, UT) an...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2007.02.018
更新日期:2007-07-01 00:00:00
abstract:INTRODUCTION:Pulp capping materials allow healing of injured pulp with a layer of reparative dentin. Glucose is needed to cure the injured area. Glucose is transported by glucose transporter (Glut) 2 and Glut4, which are transmembrane proteins that act as gatekeepers. We hypothesized that the transport of glucose via G...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2019.10.003
更新日期:2020-01-01 00:00:00
abstract::Black-pigmented bacteria (BPB) have been associated with endodontic infections. The purpose of this study was to evaluate further the presence of BPB with the clinical signs and symptoms associated with endodontic infections. Microbial samples were collected from the root canals of 40 intact teeth with necrotic pulps ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(99)80268-4
更新日期:1999-06-01 00:00:00
abstract::This study evaluated the degree of conversion of one dual-cured resin cement when used to lute fiber posts with different translucencies. To measure the degree of conversion, polyvinylsiloxane molds were prepared to simulate root canals. The posts, Aestheti-Post or Light-Post, were cemented in these molds and, after p...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2006.11.015
更新日期:2007-03-01 00:00:00
abstract::This review considers oral trauma and its relationship to orthodontics with respect to prevention and primary, secondary, and tertiary care. The level of evidence is not high in regard to this topic, but recommendable approaches to trauma at each stage are discussed on the basis of available literature and published g...
journal_title:Journal of endodontics
pub_type: 杂志文章,评审
doi:10.1016/j.joen.2012.10.030
更新日期:2013-03-01 00:00:00
abstract:INTRODUCTION:Hydrogen peroxide tooth bleaching is claimed to cause alterations in dental tissue structures. This study investigated the influence of the number of bleaching sessions on pulp tissue in rats. METHODS:Male Wistar rats were studied in 5 groups (groups 1S-5S) of 10 each, which differed by the number (1-5) o...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2013.08.007
更新日期:2013-12-01 00:00:00
abstract:INTRODUCTION:A great number of studies performed in the last 3 decades have shown the superior mechanical features of nickel-titanium files as compared with stainless steel files. A novel file system recently developed, Gentlefile, defies these findings and claims to have superior qualities despite the fact it is made ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2015.12.023
更新日期:2016-03-01 00:00:00
abstract:INTRODUCTION:Mixtures of clodronate with sodium hypochlorite (NaOCl) better maintain free available chlorine (FAC) than etidronate-hypochlorite mixtures. This research aimed to compare organic tissue dissolution and residual FAC between clodronate and etidronate mixtures. Additionally, clodronate-hypochlorite mixtures ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2019.10.020
更新日期:2020-02-01 00:00:00
abstract:INTRODUCTION:This study tested whether heat stress (42 degrees C for 30 minutes) induces reactive oxygen species (ROS), proinflammatory cytokines, Nrf2 activation, and Nrf2 target genes such as antioxidant enzymes in human dental pulp (HDP) cells. METHODS:ROS was evaluated by using flow cytometry. Proteins and messeng...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2009.06.005
更新日期:2009-09-01 00:00:00
abstract::The aim of this study was to investigate the extent of calcium ion (Ca(2+)) removal on root dentin after treatment with 1.25% NaOCl, 2.5% NaOCl, 5.25% NaOCl, 2% chlorhexidine (CHX), 2% iodine potassium iodide (IKI), and MTAD in 3 immersion time periods. Extracted human mandibular premolars were bisected longitudinally...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2008.11.016
更新日期:2009-02-01 00:00:00