Abstract:
INTRODUCTION:This study tested whether heat stress (42 degrees C for 30 minutes) induces reactive oxygen species (ROS), proinflammatory cytokines, Nrf2 activation, and Nrf2 target genes such as antioxidant enzymes in human dental pulp (HDP) cells. METHODS:ROS was evaluated by using flow cytometry. Proteins and messenger RNA levels for cytokines and antioxidant genes were determined by using Western blotting and reverse transcription-polymerase chain reaction (RT-PCR) analysis, respectively. RESULTS:Heat stress induced the production of ROS and the increased expression of the interleukin (IL)-8 and IL-8 receptor genes. Exposure of cells to heat stress resulted in the nuclear translocation of Nrf2 and increased expression of Nrf2 target genes including heme oxygenase-1. Pretreatment with an exogenous antioxidant inhibited the heat-induced expression of IL-8 and Nrf2 target genes and Nrf2 translocation. CONCLUSION:Collectively, these results show that heat-induced Nrf2 activation is the major regulatory pathway of cytoprotective gene expression against oxidative stress in HDP cells.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Chang SW,Lee SI,Bae WJ,Min KS,Shin ES,Oh GS,Pae HO,Kim ECdoi
10.1016/j.joen.2009.06.005subject
Has Abstractpub_date
2009-09-01 00:00:00pages
1222-8issue
9eissn
0099-2399issn
1878-3554pii
S0099-2399(09)00548-2journal_volume
35pub_type
杂志文章abstract:INTRODUCTION:The purpose of this in vivo study was to evaluate the accuracy of small-volume cone-beam computed tomographic (CBCT) imaging compared with conventional periapical radiography (CPR) in the diagnosis of vertical root fractures (VRFs) using exploratory surgery as the reference standard. METHODS:Eighty-two de...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2020.04.015
更新日期:2020-08-01 00:00:00
abstract:INTRODUCTION:Stabilizing sodium hypochlorite (NaOCl) at an alkaline pH is proposed to increase solution stability and tissue dissolution ability; however, a reduction on the flexural strength of dentin discs has been found to be a side effect. This study sought to determine whether a stabilized alkaline NaOCl reduces t...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2014.02.028
更新日期:2014-10-01 00:00:00
abstract::This study compared the cytotoxicity of MetaSEAL (Parkell Inc, Farmington, NY), a methacrylate resin-based sealer with an epoxy resin-based (AH Plus Jet; Dentsply Caulk, Milford, DE) and a zinc oxide-eugenol-based sealer (Pulp Canal Sealer; SybronEndo, Orange, CA). Five-millimeter diameter disks prepared from the resp...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2008.06.020
更新日期:2008-09-01 00:00:00
abstract::The aim of this study was to evaluate the presence of bacterial biofilm on the external surface of the root apex in teeth with pulp necrosis, with and without radiographically visible periapical lesions, and in teeth with a vital pulp. Twenty-one teeth were extracted, eight with pulp necrosis and periapical lesions, e...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200212000-00006
更新日期:2002-12-01 00:00:00
abstract::In this study the sealing ability of a new urethane methacrylate resin-based sealer, EndoRez, was evaluated using a fluid-filtration model. Sixty-four single-rooted lower bicuspids were decoronated, instrumented, and divided into 3 groups of 20 each with 4 teeth used as controls. In group A, the roots were obturated w...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200310000-00011
更新日期:2003-10-01 00:00:00
abstract::A degree of uncertainty exists about the use of cotton pellets in the pulp chamber after canal obturation. The purpose of this study was to poll practicing endodontists and generalists regarding their preference. Forty-six endodontists and an equal number of general dentists were polled in a mail survey regarding thei...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200209000-00007
更新日期:2002-09-01 00:00:00
abstract::A lack of agreement exists in the literature regarding the anatomical relationship of the mandibular canal to its surrounding structures such as the root apices. The purpose of this investigation was to study the spatial relationship of the mandibular canal to the posterior teeth in dried human mandibles. Twenty-two m...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(06)81411-1
更新日期:1992-04-01 00:00:00
abstract::A polydimethylsiloxane-based sealer (GuttaFlow) was used for filling single-rooted teeth with four obturation techniques. Prefitted gutta-percha master-cones were employed in groups 1 to 3 with backfilling of the sealer. Apical third of the roots were first filled with GuttaFlow, and sealer-coated master cones were se...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2006.07.009
更新日期:2006-12-01 00:00:00
abstract::Biopsy reports of specimens from the apices of 1659 teeth physically present at the time the biopsy was performed were reviewed. The following information was recorded from each report: (a) source of specimen, (b) sex of patient, (c) age of patient, (d) location of tooth associated with lesion, and (e) diagnoses repor...
journal_title:Journal of endodontics
pub_type: 杂志文章,评审
doi:10.1016/s0099-2399(06)81679-1
更新日期:1990-05-01 00:00:00
abstract::It is a general belief that large cyst-like periapical lesions and apical true cysts caused by root canal infection are less likely to heal after nonsurgical root canal therapy. Nevertheless, there is no direct evidence to support this assumption. A large cyst-like periapical lesion or an apical true cyst is formed wi...
journal_title:Journal of endodontics
pub_type: 杂志文章,评审
doi:10.1016/j.joen.2009.02.012
更新日期:2009-05-01 00:00:00
abstract::The aim of this study was to investigate the response of human pulp tissue to a dentin bonding agent, Scotchbond Multi-Purpose Plus (SMPP), in exposed class V cavities. Sixteen human premolar teeth were mechanically exposed. Ten pulps were capped with SMPP and six teeth were capped with Dycal. The cavities were filled...
journal_title:Journal of endodontics
pub_type: 临床试验,杂志文章
doi:10.1097/01.don.0000137649.24821.91
更新日期:2005-03-01 00:00:00
abstract:INTRODUCTION:Achieving anesthesia in mandibular molar teeth with irreversible pulpitis is very difficult. The aim of this study was to compare the efficacy of 1.8 mL and 3.6 mL articaine for an inferior alveolar nerve block (IANB) when treating molars with symptomatic irreversible pulpitis. METHODS:In a randomized, do...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2015.05.015
更新日期:2015-09-01 00:00:00
abstract:INTRODUCTION:The mental foramen (MF) is an important landmark in dentistry. Knowledge of its position is central to perform block anesthesia of the mental nerve or to avoid nerve damage during surgical procedures in the premolar area of the mandible. The present radiographic study aimed at evaluating the location and d...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2013.07.033
更新日期:2013-12-01 00:00:00
abstract:INTRODUCTION:This study aimed at evaluating the bond strength to root dentin of 2 mineral trioxide aggregate (MTA)-based sealers (Endo-CPM sealer and MTA Fillapex) and of 1 epoxy resin-based sealer (AH Plus sealer). METHODS:Forty-five extracted human teeth with single roots were prepared by using the step-back techniq...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2011.10.018
更新日期:2012-02-01 00:00:00
abstract::Silicone tubes filled with CRCS, a calcium hydroxide-based cement, were implanted at two different localizations in the right tibias of white male Wistar rats. A similar number of solid silicone rods were implanted in the left tibias of the same animals and were used as inert controls. Fifteen days after implantation ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(89)80090-1
更新日期:1989-01-01 00:00:00
abstract::Characteristics of cyclooxygenase-2 (COX-2) expressing cells in human dental pulp were immunohistologically studied. Extirpated pulpal tissues from extracted teeth were examined to elucidate the localization and distribution of COX-2. Pulpal tissues were examined by the labeled streptavidin biotin method using specifi...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200106000-00003
更新日期:2001-06-01 00:00:00
abstract::Pulp regeneration and its clinical translation into regenerative endodontic procedures are receiving increasing research attention, leading to significant growth of the published scientific and clinical literature within these areas. Development of research strategies, which consider patient-, clinician-, and scientis...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2017.06.002
更新日期:2017-09-01 00:00:00
abstract:INTRODUCTION:Electrically heated pluggers are the most commonly used instruments during warm obturation techniques. This study aimed to evaluate the effect of sterilization and operating temperature settings on the heat generation of pluggers of various taper sizes. METHODS:Fifty pluggers were sterilized at 132°C for ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2019.09.008
更新日期:2019-12-01 00:00:00
abstract:INTRODUCTION:The diagnosis and treatment planning of cracked teeth depend on the understanding of how cracks affect the surrounding tissues. This study evaluated the dentin and pulp conditions in teeth affected by cracks and attrition. METHODS:Specimens under investigation included 12 cracked posterior teeth and 8 tee...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2014.09.021
更新日期:2015-03-01 00:00:00
abstract:INTRODUCTION:This randomized clinical trial assessed the effect of preoperative intraoral cryotherapy application on the success rate of inferior alveolar nerve blocks (IANBs) in patients with symptomatic irreversible pulpitis (SIP). METHODS:One hundred four patients with SIP were randomly distributed into 2 groups: c...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2019.05.001
更新日期:2019-08-01 00:00:00
abstract::This study evaluated the effect of three post surface treatments on quartz-fiber post (a) retention and (b) morphology. A control group was not treated. Different treatments were performed for the other posts: silanization; etching by hydrofluoric acid 9.5%; sandblasting with 50 microm Al2O3. Two specimens of each gro...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2006.10.015
更新日期:2007-03-01 00:00:00
abstract::The biocompatibility and apical microleakage of tricalcium phosphate based Sankin Apatite (SA) Type I, II, and III root canal sealers were investigated. Teflon tubes containing freshly mixed test materials were implanted in the subcutaneous tissue of mice. The observation periods were 24 h, 7, and 30 days, after which...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/s0099-2399(97)80255-5
更新日期:1997-02-01 00:00:00
abstract::Orthodontic tooth movement has been implicated in secondary changes to the dental pulp. The purpose of this study was to correlate the effects of orthodontic tooth movement on the dental pulp by histomorphometric parameters. Four groups, each consisting of 36 male adult Sprague-Dawley strain rats, were studied with di...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(06)81034-4
更新日期:1993-01-01 00:00:00
abstract:INTRODUCTION:Mineral trioxide aggregate (MTA) and calcium silicate (CS) cements exhibit acceptable levels of cytocompatibility. The aim of the present study was to evaluate MTA and CS cement genotoxicity in a human osteosarcoma cell line (MG63). METHODS:A mitochondrial colorimetric assay was used to evaluate the MG63 ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2010.03.039
更新日期:2010-07-01 00:00:00
abstract:PURPOSE:To evaluate in vitro the solubility of the Epiphany endodontic filling material Epiphany (Pentron Clinical Technologies, Wallingford, CT) prepared with its resinous solvent. METHODS:The specimens were prepared in the following experimental conditions: (1) GI, epiphany without photoactivation; (2) GII, Epiphany...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2009.01.021
更新日期:2009-05-01 00:00:00
abstract:INTRODUCTION:The purpose of this in vitro study was to compare the cytotoxicity of white mineral trioxide aggregate cement (AMTA, MTA-Angelus), Brasseler Endosequence Root Repair Putty (ERRM), Dycal, and Ultra-blend Plus (UBP) by using human dermal fibroblasts and a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2011.11.012
更新日期:2012-03-01 00:00:00
abstract:INTRODUCTION:Anesthetic efficacy of inferior alveolar nerve block decreases in patients with irreversible pulpitis. It was hypothesized that premedication with nonsteroidal anti-inflammatory drugs might improve the success rates in patients with inflamed pulps. METHODS:Sixty-nine adult volunteers who were actively exp...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2009.11.010
更新日期:2010-03-01 00:00:00
abstract::Enterococcus faecalis has been suggested to be an important etiological agent in endodontic failures. The purpose of this study was to evaluate the effectiveness of chlorhexidine- or antibiotics-based medications in eliminating E. faecalis biofilms. One-day and three-day biofilms of E. faecalis were induced on cellulo...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200110000-00004
更新日期:2001-10-01 00:00:00
abstract::This study compares the incidence, degree, and length of postoperative pain in 300 endodontically treated teeth, with and without apical patency, in relation to some diagnostic factors (vitality, presence of preoperative pain, group, and mandible of treated tooth). Of the questionnaires received back, apical patency w...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2008.11.014
更新日期:2009-02-01 00:00:00
abstract::Bisphosphonates are commonly used in medicine to maintain bone density in patients with certain nonneoplastic diseases or cancers. A serious adverse effect of bisphosphonates that has substantial dental significance is osteonecrosis that appears to uniquely affect the mandible and maxilla without occurring in other bo...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/01.don.0000186481.96169.cd
更新日期:2005-11-01 00:00:00