Heat stress activates interleukin-8 and the antioxidant system via Nrf2 pathways in human dental pulp cells.

Abstract:

INTRODUCTION:This study tested whether heat stress (42 degrees C for 30 minutes) induces reactive oxygen species (ROS), proinflammatory cytokines, Nrf2 activation, and Nrf2 target genes such as antioxidant enzymes in human dental pulp (HDP) cells. METHODS:ROS was evaluated by using flow cytometry. Proteins and messenger RNA levels for cytokines and antioxidant genes were determined by using Western blotting and reverse transcription-polymerase chain reaction (RT-PCR) analysis, respectively. RESULTS:Heat stress induced the production of ROS and the increased expression of the interleukin (IL)-8 and IL-8 receptor genes. Exposure of cells to heat stress resulted in the nuclear translocation of Nrf2 and increased expression of Nrf2 target genes including heme oxygenase-1. Pretreatment with an exogenous antioxidant inhibited the heat-induced expression of IL-8 and Nrf2 target genes and Nrf2 translocation. CONCLUSION:Collectively, these results show that heat-induced Nrf2 activation is the major regulatory pathway of cytoprotective gene expression against oxidative stress in HDP cells.

journal_name

J Endod

journal_title

Journal of endodontics

authors

Chang SW,Lee SI,Bae WJ,Min KS,Shin ES,Oh GS,Pae HO,Kim EC

doi

10.1016/j.joen.2009.06.005

subject

Has Abstract

pub_date

2009-09-01 00:00:00

pages

1222-8

issue

9

eissn

0099-2399

issn

1878-3554

pii

S0099-2399(09)00548-2

journal_volume

35

pub_type

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