Abstract：
:Neutrophils make up an essential part of the innate immune system, and are involved both in the initial responses to pathogens, and in orchestrating later immune responses. Neutrophils recognize pathogens through pattern-recognition receptors (PRRs), which are activated by microbial motifs. The Nod-like receptors (nucleotide-binding domain leucine-rich repeat containing family; NLRs) constitute a recently discovered group of PRRs whose role in the neutrophil immune responses is not yet characterized. The present study aimed to investigate the expression and function of NLRs in neutrophils. Neutrophils were isolated from human peripheral blood, and the presence of nucleotide-binding oligomerization domain 1 (NOD1), NOD2 and NACHT-LRR-PYD-containing protein 3 (NLRP3) was evaluated with flow cytometry and immunohistochemistry. The expression of NOD1, NOD2 and NLRP3 messenger RNA was determined using real-time reverse transcription-polymerase chain reaction. Changes in neutrophil cytokine secretion, phenotype and migration following agonist-induced activation were studied using enzyme-linked immunosorbent assay, flow cytometry and a chemotaxis assay, respectively. No expression of NOD1 was found in isolated neutrophils and stimulation with the NOD1 ligand gamma-d-glutamyl-meso-diaminopimelic acid induced no signs of activity. In contrast, a marked expression of NOD2 and NLRP3 was found. NOD2 activation with MurNAc-l-Ala-d-isoGln (MDP) resulted in interleukin-8 secretion, CD62 ligand down-regulation, CD11b up-regulation and increased migration towards an inflammatory stimulus. NLRP3 activation with alum caused interleukin-1beta secretion and facilitated migration. Altogether, this suggests that NLRs may be a previously unknown pathway for neutrophil activation.
journal_name
Immunologyjournal_title
Immunologyauthors
Ekman AK,Cardell LOdoi
10.1111/j.1365-2567.2009.03212.xsubject
Has Abstractpub_date
2010-05-01 00:00:00pages
55-63issue
1eissn
0019-2805issn
1365-2567pii
IMM3212journal_volume
130pub_type
杂志文章相关文献
IMMUNOLOGY文献大全abstract：:The amount and relative affinity of antibodies to tetanus toxoid were measured, following immunization, in patients with chronic or recurrent acute chest infections and in healthy controls. The responding patients and controls produced similar amounts of antibody and, although antibody affinity was higher in the contr...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1985-07-01 00:00:00
abstract：:Suppressor T (Ts) cells specific for minor alloantigens can be generated by intravenous administration of high doses (10(8) cells) of alloantigenic spleen cells. Such Ts cells have previously been shown to inhibit the in vivo induction of CTL responses and are now shown to suppress in vitro proliferative responses to ...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1989-10-01 00:00:00
abstract：:Mucosa-associated invariant T (MAIT) cells express the semi-invariant T-cell receptor TRAV1-2 and detect a range of bacteria and fungi through the MHC-like molecule MR1. However, knowledge of the function and phenotype of bacteria-reactive MR1-restricted TRAV1-2(+) MAIT cells from human blood is limited. We broadly ch...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/imm.12461
更新日期：2015-07-01 00:00:00
abstract：:In the past 15 years, regulatory T cell (Treg) suppression has graduated from a phenomenon that 'dare not speak its name' to a field at the centre of a global research effort. It is now accepted that Tregs can target numerous cell populations to elicit potent immunosuppression. Intriguingly, emerging data suggest that...
journal_title：Immunology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-2567.2009.03053.x
更新日期：2009-04-01 00:00:00
abstract：:Polystyrene surfaces coated with proteins at alkaline pH were found to be useful to investigate the stimulation of neutrophils independently of phagocytosis. Human neutrophils, when exposed to appropriate stimuli, release superoxide anion (O2-). We have measured superoxide dismutase-inhibitable cytochrome c reduction ...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1978-10-01 00:00:00
abstract：:Various strains of the murine mycoplasma M. neurolyticum have been shown to induce extensive blast transformation of mouse lymphocytes, comparable in strength to mitogenicity exerted by these mycoplasma species on rat lymphocytes. The data summarized in this report demonstrate that this mitogenic effect is non-specifi...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1978-04-01 00:00:00
abstract：:The intestinal epithelium is rich in γδ T cells and the gut is a site of residence for a wide variety of pathogens, including nematodes. Although CD4+ T-cell receptor (TCR) -αβ+ T helper type 2 T cells are essential for the expulsion of intestinal nematodes, little information is available on the function of γδ T cell...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2011.03503.x
更新日期：2011-12-01 00:00:00
abstract：:Immunodeficiency syndromes associated with protein-energy malnutrition (PEM) have been documented extensively, although to date the mechanism underlying these defects remains uncharacterized. In this study, we have evaluated T, B, and antigen-presenting cell functions of malnourished mice fed a 4% protein diet compare...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1988-04-01 00:00:00
abstract：:The purpose of this work was to determine if the fine specificity of T cells differed between mice immunized with an antigen in a T helper 1 (Th1) cytokine-dominated environment as compared with a T helper 2 (Th2) cytokine-dominated environment. It was found that splenic T cells from mice immunized with mycobacterial ...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.1998.00007.x
更新日期：1998-04-01 00:00:00
abstract：:Using cloned human helper T lymphocytes reactive with a 24 amino acid peptide (p20) of the carboxyl terminal of the HA-1 molecule of influenza haemagglutinin we have investigated the influence of antigen structure on the activation and in the induction of antigen specific unresponsiveness of T cells. For this analysis...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1986-03-01 00:00:00
abstract：:We produced three murine monoclonal antibodies (mAb) directed against the human T-cell differentiation antigen CD2 (formerly T11) and analysed their epitope specificity by E-rosette inhibition, cross-blocking and proliferation-induction experiments. When added together, two mAb (both IgG1 kappa) were able to induce pr...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1989-07-01 00:00:00
abstract：:Large numbers of cells can be recovered from pig intestine with phenotypes suggesting lamina propria rather than intraepithelial origin. Following activation with concanavalin A these cells produced a T-cell growth factor (TCGF) activity which was not inhibited in the presence of a monoclonal antibody recognizing pig ...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1994-08-01 00:00:00
abstract：:In many species, the rejection of liver allografts is milder than that of other organs. This is especially so in the rat where, without immunosuppressive treatment, liver grafts between certain strain combinations are accepted permanently, whereas skin, heart and renal allografts undergo acute rejection. Reliable surg...
journal_title：Immunology
pub_type： 杂志文章,评审
doi：
更新日期：1985-07-01 00:00:00
abstract：:The cellular prion protein (PrPc) is a glycosyl-phosphatidylinositol (GPI)-linked cell surface protein, which is expressed at high density on nervous tissues and at lower levels on most other solid-organ tissues. It is also expressed on peripheral blood mononuclear cells (PBMC) of all lineages. In lymphocytes, its lev...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.2001.01166.x
更新日期：2001-02-01 00:00:00
abstract：:Cellular adhesion molecules play a central role in leucocyte migration through peripheral blood and tissues. A crucial stage in these events in selectin-mediated adhesion involving E-selectin expressed on activated endothelium interacting with a range of carbohydrate ligands expressed by specific subpopulations of leu...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1994-02-01 00:00:00
abstract：:The influence of intact influenza virus and purified detergent solubilized haemagglutinin (HA) subunits from these viruses on human natural killer (NK) cell activity was examined. Effector cells incubated with whole influenza virus for 18 hr initiated the production of alpha interferon which was associated with the en...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1984-08-01 00:00:00
abstract：:The kinetics of anti-paternal alloantibody production in syngeneically and allogeneically mated mice previously immunized with allogenic spleen cells has been investigated. In allogeneically mated females, total and cytotoxic alloantibody levels increased during the final 3 days of pregnancy to reach maximal levels ar...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1982-05-01 00:00:00
abstract：:The adoptive transfer of immunity by means of spleen and lymph node cells has been analysed in rabbits of defined major histocompatibility (RLA) types and immunoglobulin (Ig) allotypes. Previous results had shown that chimaeras formed by transplanting adult cells into RLA-matched newborn hosts become stable B-lymphocy...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1987-01-01 00:00:00
abstract：:Nasal carriage of Staphylococcus aureus is an important source of nosocomial infection and community-acquired methicillin-resistant S. aureus (MRSA). Previous studies by our laboratory revealed that nasal carriage of S. aureus is accompanied by subclinical inflammation, which is insufficient to prevent colonization, a...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2007.02615.x
更新日期：2007-09-01 00:00:00
abstract：:The role of anti-idiotypic subclass-specific antibodies was analysed in the regulation of the immune response to BCG in the guinea-pig. The idiotypes to BCG were separated into subclasses and anti-idiotypes were carried out by immunizing with the purified IgG1 and IgG2 anti-idiotypes. The in vivo T cell response was r...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1984-08-01 00:00:00
abstract：:Polyclonal rabbit anti-idiotypic (Ab2) antibodies raised against the antiprogesterone mAb DB3 (Ab1) were used to induce an Ab3 antiprogesterone response in BALB/c mice. While the affinity of Ab3 sera for progesterone was 10-50-times lower than that of DB3, their steroid-binding specificity showed considerable similari...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.2000.00006.x
更新日期：2000-06-01 00:00:00
abstract：:The mammalian target of rapamycin (mTOR) pathway is an important integrator of nutrient-sensing signals in all mammalian cells, and acts to coordinate the cell proliferation with the availability of nutrients such as glucose, amino acids and energy (oxygen and ATP). A large part of the immune response depends on the p...
journal_title：Immunology
pub_type： 杂志文章,评审
doi：10.1111/imm.12162
更新日期：2013-12-01 00:00:00
abstract：:T cells do not generally express Fc receptors (FcRs). However, we report here that C8166 cells, a human CD4+ T lymphoblastoid cell line, widely used in research into the human immunodeficiency virus type 1 (HIV-1), expressed CD64 (Fc gamma RI), CD32 (Fc gamma RII), and CD16 (Fc gamma RIII) on the plasma membrane as sh...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.1997.00116.x
更新日期：1997-01-01 00:00:00
abstract：:The mechanisms of action and the nature of the co-operating cells (CC) controlling human T-lymphocyte-colony formation were investigated. Media conditioned by PHA-stimulated blood mononuclear cells (MC) were tested for their capacity to induce T-colony formation in the effluent cell population, obtained after anti (Fa...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1982-02-01 00:00:00
abstract：:Antibody-dependent cell-mediated cytotoxicity (ADCC) of infectious bovine rhinotracheitis (IBR)-infected bovine kidney cells (MDBK) by neutrophils was demonstrated. Neutrophils from bovine and sheep mammary exudate and peripheral blood, and also from human peripheral blood, were all active in the presence of anti-IBR ...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1984-10-01 00:00:00
abstract：:Human β-defensin 3 (hBD-3) activates antigen-presenting cells through Toll-like receptors (TLRs) 1/2. Several TLR1/2 agonists have been identified but little is known about how they might differentially affect cellular activation. We compared the effects of hBD-3 with those of another TLR1/2 agonist, Pam(3) CSK(4) , i...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2011.03475.x
更新日期：2011-10-01 00:00:00
abstract：:Mice injected intravenously with a high dose (5 X 10(7) ) of BCG fail to develop delayed hypersensitivity to BCG and are described as anergic or unresponsive. Spleen cells from these mice release factors on culture which suppress DNA synthesis induced by concanavalin A in vitro. Cell separation experiments showed that...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1984-01-01 00:00:00
abstract：:The members of the tumour necrosis factor (TNF) superfamily of cytokines play important roles in the regulation of various immune-cell functions. Likewise, induction of cell death by apoptosis is indispensable for the normal functioning of the immune system. There are two major pathways of apoptosis induction. The int...
journal_title：Immunology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-2567.2009.03058.x
更新日期：2009-06-01 00:00:00
abstract：:Interleukin-2 (IL-2) is a critical regulator of immune homeostasis through its non-redundant role in regulatory T (Treg) cell biology. There is major interest in therapeutic modulation of the IL-2 pathway to promote immune activation in the context of tumour immunotherapy or to enhance immune suppression in the contex...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/imm.12609
更新日期：2016-07-01 00:00:00
abstract：:We have isolated, characterized and quantified the immunocompetent cells present in the extravascular hepatic compartment at various stages after Plasmodium yoelii malaria infection with sporozoites. Cytological analyses revealed a predominantly lymphoid population. In mice with a primary infection, the predominant ce...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1994-08-01 00:00:00
