Abstract:
INTRODUCTION:We have previously described the labeling of interleukin-2 (IL2) with (123)I and (99m)Tc-N(3)S. Both radiopharmaceuticals were successfully applied in humans to image several inflammatory lesions and autoimmune diseases characterized by tissue infiltrating lymphocytes expressing the IL2 receptor (CD25). However, both radiopharmaceuticals had some specific disadvantages, such as cost and time of synthesis. MATERIALS AND METHODS:Here, we describe a new improved method for labeling interleukin-2 with (99m)Tc using HYNIC-NHS and tricine as coligand. Several optimizations of reagent concentrations and labeling conditions were performed in order to standardize the procedure. After labeling, IL2 was purified by tC2 reverse-phase chromatography and tested in vitro and in vivo, in mice and in a normal volunteer. Results showed that this labeling procedure is cheap, fast, reliable, and reproducible, leading to a product with high specific activity (153 µCi/µg), high stability and capable of binding in vitro to CD25 positive cells. In vivo biodistribution in mice and human volunteer did not show any significant different from (99m)Tc-N(3)S-IL2. CONCLUSION:In conclusion, the optimization of (99m)Tc-HYNIC-IL2 has a great advantage in terms of cost and time of production and a simple kit formulation can be considered for routine application to study patients affected by autoimmune diseases, graft rejection, or other chronic inflammatory disorders.
journal_name
Mol Imaging Bioljournal_title
Molecular imaging and biologyauthors
D'Alessandria C,di Gialleonardo V,Chianelli M,Mather SJ,de Vries EF,Scopinaro F,Dierck RA,Signore Adoi
10.1007/s11307-009-0285-1subject
Has Abstractpub_date
2010-10-01 00:00:00pages
539-46issue
5eissn
1536-1632issn
1860-2002journal_volume
12pub_type
杂志文章abstract:PURPOSE:Manganese ion has been extensively used as a magnetic resonance imaging (MRI) contrast agent in preclinical studies to assess tissue anatomy, function, and neuronal connectivity. Unfortunately, its use in human studies has been limited by cellular toxicity and the need to use a very low dose. The much higher se...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-018-1162-6
更新日期:2018-08-01 00:00:00
abstract:INTRODUCTION:Technetium (Tc) methylene diphosphonate (MDP) has been the standard method for bone scintigraphy for three decades. (18)F sodium fluoride ((18)F NaF) positron emission tomography (PET)/computed tomography (CT) has better resolution and is considered superior. The role of 2-deoxy-2-[(18)F]fluoro-D-glucose (...
journal_title:Molecular imaging and biology
pub_type: 临床试验,杂志文章
doi:10.1007/s11307-011-0486-2
更新日期:2012-04-01 00:00:00
abstract:PURPOSE:[(18)F]-FEPPA is a translocator protein (18 kDa, TSPO) positron emission tomography (PET) radiotracer. Radiation dosimetry was estimated from the whole body biodistribution, taking into consideration TSPO rs6971 (Ala147Thr) polymorphism. PROCEDURES:[(18)F]-FEPPA whole body PET scans were acquired for six healt...
journal_title:Molecular imaging and biology
pub_type: 临床试验,杂志文章
doi:10.1007/s11307-012-0589-4
更新日期:2013-06-01 00:00:00
abstract:PURPOSE:The increasing availability of fluorescent probes for in vivo optical imaging enables the interrogation of complex biological processes in small animals serving as models for human-like tissue function and disease. However, the validation of probe bio-distribution during their development or the study of differ...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-010-0416-8
更新日期:2011-10-01 00:00:00
abstract:OBJECTIVES:Accurate staging of patients with melanoma is vital to guide appropriate treatment. 2-Deoxy-2-[F-18]fluoro-D-glucose (FDG)-positron emission tomography (PET) has been reported to be a sensitive and specific technique for the staging of advanced melanoma, however, few studies provide information regarding its...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-005-0002-7
更新日期:2005-07-01 00:00:00
abstract:PURPOSE:The purpose of this paper is to validate a rapid and cost-effective ex vivo technique, microCT-based virtual histology, as an alternative to MRI imaging for assessing the therapeutic response in genetically engineered mouse models of cancer. PROCEDURES:All animal procedures were conducted in accordance with th...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-010-0372-3
更新日期:2011-06-01 00:00:00
abstract:PURPOSE:CXCR4 is overexpressed on tumor cells from many types of human cancers. A high level of CXCR4 expression often correlates with poor prognosis, chemotherapy resistance, and metastasis. The development of CXCR4-specific radiotracers for positron emission tomography (PET) imaging will allow in vivo evaluation of r...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-013-0640-0
更新日期:2013-12-01 00:00:00
abstract:PURPOSE:Macrophage accumulation characterizes the development of atherosclerotic plaques, and the presence of certain macrophage subsets might be an indicator of plaque phenotype and (in)stability. The macrophage mannose receptor (MMR) is expressed on alternatively activated macrophages and found at sites of intraplaqu...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-017-1117-3
更新日期:2018-04-01 00:00:00
abstract:PURPOSE:This study aims to determine a lumped constant (LC) value that can be applied to the 2-deoxy-2[18F]fluoro-D-glucose positron emission tomography (FDG-PET) study to yield a physiological value of cerebral metabolic rate of glucose (CMR(glc)) in normal brain. PROCEDURES:We developed a more robust method for dete...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1016/s1536-1632(02)00122-1
更新日期:2003-01-01 00:00:00
abstract::This paper demonstrates the application of mutual information based coregistration of radionuclide and magnetic resonance imaging (MRI) in an effort to use multimodality imaging for noninvasive localization of stem cells grafted in the infarcted myocardium in rats. Radionuclide imaging such as single photon emission c...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-006-0062-3
更新日期:2007-01-01 00:00:00
abstract:PURPOSE:This study aimed to synthesize a necrosis-avid agent using rhein as a precursor and labeled with gallium-68 (Ga-68) for positron emission tomography/computed tomography (PET/CT) imaging, to evaluate response to anticancer treatment in a mouse model. PROCEDURES:1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraaceti...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-019-01365-y
更新日期:2020-06-01 00:00:00
abstract:PURPOSE:Inflammation plays a key role in the progression of intervertebral disk (IVD) herniation and associated low back pain. However, real-time spatial diagnosis of inflammation associated with acute disk herniation has not been investigated. We sought to detect local neutrophil and macrophage infiltration near disk ...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-016-1038-6
更新日期:2017-10-01 00:00:00
abstract:PURPOSE:Noninvasive imaging of cell necrosis can provide an early evaluation of tumor response to treatments. Here, we aimed to design and synthesize a novel diindole-based magnetic resonance imaging (MRI) contrast agent (Gd-bis-DOTA-diindolylmethane, Gd-DIM) for assessment of tumor response to therapy at an early stag...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-019-01399-2
更新日期:2020-06-01 00:00:00
abstract:PURPOSE:The aims of this study were to evaluate the binding and pharmacokinetics of novel 18F-labeled ethenyl-benzoxazole derivatives (i.e., [18F] fluorinated amyloid imaging compound of Tohoku university ([18F]FACT)) as amyloid positron emission tomography (PET) tracers and to assess [18F]FACT efficacy in imaging of A...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-012-0608-5
更新日期:2013-08-01 00:00:00
abstract:PURPOSE:Previously, some reports mentioned that magnetic resonance imaging (MRI) can predict histopathological features in primary CNS lymphoma (PCNSL). The reported data analyzed diffusion-weighted imaging findings. The aim of this study was to investigate possible associations between histopathological findings, such...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-017-1115-5
更新日期:2018-04-01 00:00:00
abstract:PURPOSE:Negative surgical margins (NSMs) have favorable prognostic implications in breast tumor resection surgery. Fluorescence image-guided surgery (FIGS) has the ability to delineate surgical margins in real time, potentially improving the completeness of tumor resection. We have recently developed indocyanine green ...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-019-01462-y
更新日期:2020-08-01 00:00:00
abstract:PURPOSE:Ga-68-labeled prostate-specific membrane antigen (PSMA) ligands have been used clinically for positron emission tomography (PET) imaging of prostate cancer. However, F-18-labeled compounds offer several advantages, including the potential for delayed imaging, high starting activities enabling multidose preparat...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-015-0844-6
更新日期:2015-12-01 00:00:00
abstract::Magnetic resonance imaging (MRI) requires synthesis of contrast media bearing targeting groups and numerous gadolinium chelating groups generating high relaxivity. This paper explores the results of linking the gadolinium chelates to the targeting group, a protein molecule, via various types of linkers. Polycondensate...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-010-0358-1
更新日期:2011-06-01 00:00:00
abstract::The cyclooxygenase-2 (COX-2) gene plays a role in a wide variety of normal physiologic pathways and is a major target of pharmacologic intervention in a large number of pathophysiologic contexts, including pain, fever, inflammation, and cancer. Expression of the COX-2 gene is induced in a wide range of cells, in respo...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1016/s1536-1632(03)00105-7
更新日期:2003-07-01 00:00:00
abstract:PURPOSE:The present work was aimed at the formulation and evaluation of freeze-dried kits of 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA)-peptides for the preparation of (68)Ga-labelled peptides for PET imaging of neuroendocrine tumours. The (68)GaCl3 was obtained from the locally produced nanoceria-...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-014-0719-2
更新日期:2014-08-01 00:00:00
abstract:PURPOSE:Increased expression of the αvβ6 integrin correlates with advanced tumor grade and poor clinical outcome, identifying αvβ6 as a prognostic indicator and an attractive target for molecular imaging. This work investigated the ability of a disulfide-stabilized [64Cu]NOTA-αvβ6 cys-diabody to image αvβ6 expression i...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-017-1097-3
更新日期:2018-02-01 00:00:00
abstract:PURPOSE:To evaluate prognostic value of pretreatment and posttreatment 2-deoxy-2-[18F]fluoro-D-glucose positron emission tomography (FDG-PET) in advanced hypopharyngeal carcinoma treated by chemoradiotherapy. PROCEDURES:Thirty-one patients underwent a baseline FDG-PET and then FDG-PET was repeated 7 weeks after chemor...
journal_title:Molecular imaging and biology
pub_type: 临床试验,杂志文章
doi:10.1007/s11307-009-0229-9
更新日期:2010-01-01 00:00:00
abstract:PURPOSE:The goal of this study was to determine the specificity of ⁶⁴Cu-CB-TE2A-c(RGDyK) (⁶⁴Cu-RGD) for osteoclast-related diseases, such as Paget's disease or rheumatoid arthritis. PROCEDURES:C57BL/6 mice were treated systemically with osteoprotegerin (OPG) for 15 days or RANKL for 11 days to suppress and stimulate o...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-011-0512-4
更新日期:2012-08-01 00:00:00
abstract:PURPOSE:[123I]FP-CIT (DaTSCAN®) single-photon emission computed tomography (SPECT) imaging is widely used to study neurodegenerative parkinsonism, by measuring presynaptic dopamine transporter (DAT) in striatal regions. Beyond DAT, [123I]FP-CIT may be considered for other monoaminergic systems, in particular the seroto...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-017-1052-3
更新日期:2017-10-01 00:00:00
abstract:PURPOSE:This study was designed to estimate the clinical significance of the contrast-enhanced computed tomography (CT) textural features for prediction of survival in colorectal cancer (CRC) patients receiving targeted therapy (bevacizumab and cetuximab). PROCEDURES:The LifeX software was used to extract the textural...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-020-01552-2
更新日期:2020-10-27 00:00:00
abstract:PURPOSE:To explore a representative hypoxic parameter to predict the treatment response and prognosis for [18F]fluoromisonidazole ([18F]FMISO) positron emission tomography (PET)/X-ray computed tomography (CT) in patients with non-small cell lung cancer (NSCLC). PROCEDURES:Twenty-nine patients with NSCLC underwent FMIS...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-018-1190-2
更新日期:2018-12-01 00:00:00
abstract:PURPOSE:The purpose of this study is to evaluate if the differential exchange rates with bulk water between amine and amide protons can be exploited using chemical exchange saturation transfer magnetic resonance (CEST-MR) to monitor the release of glutamate induced by carboxypeptidase G2 (CPG2), an enzyme utilized in c...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-013-0680-5
更新日期:2014-04-01 00:00:00
abstract:PURPOSE:Evaluation of [68Ga]NODAGA-duramycin as a positron emission tomography (PET) tracer of cell death for whole-body detection of chemotherapy-induced organ toxicity. PROCEDURES:Tracer specificity of Ga-68 labeled NODAGA-duramycin was determined in vitro using competitive binding experiments. Organ uptake was anal...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-019-01417-3
更新日期:2020-06-01 00:00:00
abstract:PURPOSE:[(18)F]Flumazenil, which has the advantage of a longer half-life than [(11)C]flumazenil, is well known for determining of the central benzodiazepine receptor concentrations. However, [(18)F]flumazenil has not been widely used because fluctuating and relatively low yields render automatic production insufficient...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-014-0738-z
更新日期:2014-10-01 00:00:00
abstract:PURPOSE:Sensitivity of contrast-enhanced ultrasound (CEUS) to microvascular flow modifications can be limited by intra-injection variability (injected dose, rate, volume). PROCEDURES:To evaluate the effect of injection variability on microvascular flow evaluation, CEUS was compared between controlled and manual inject...
journal_title:Molecular imaging and biology
pub_type: 杂志文章
doi:10.1007/s11307-016-0952-y
更新日期:2016-10-01 00:00:00