Abstract：
:Two-cell mouse embryos were cultured in Ham's F-10 medium (Gibco, Grand Island, NY) supplemented with various concentrations of transferrin, insulin, epidermal growth factor, platelet-derived growth factor, or fibroblast growth factor. Rates of development and the cell numbers per blastocyst were compared between embryos cultured in Ham's F-10 alone versus growth factor (GF) supplemented media. Rates of development in the presence of GF were inhibited significantly in some cases, and the cell numbers per blastocyst were identical in all groups. When compared with in vivo derived blastocysts, however, all in vitro cultured embryos had significantly less cells per embryo. These results suggest that GF supplementation of media for culture of early preimplantation embryos does not stimulate the rate of embryonic development in vitro.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Colver RM,Howe AM,McDonough PG,Boldt Jdoi
10.1016/s0015-0282(16)54082-5subject
Has Abstractpub_date
1991-01-01 00:00:00pages
194-9issue
1eissn
0015-0282issn
1556-5653pii
S0015-0282(16)54082-5journal_volume
55pub_type
杂志文章abstract：:A prospective randomized study of 243 embryo transfers revealed that the use of blastocysts in assisted reproductive technology is not more effective than the use of day 2 and day 3 embryos. ...
journal_title：Fertility and sterility
pub_type： 临床试验,信件,随机对照试验
doi：10.1016/j.fertnstert.2003.07.016
更新日期：2004-02-01 00:00:00
abstract：:We have microsurgically extracted a pronucleus from 11 tripronuclear human zygotes under two different experimental conditions. One group of six zygotes was incubated in cytochalasin D for 30 minutes before manipulation. The remaining five zygotes were manipulated without preincubation in cytoskeletal relaxing agents....
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)60901-9
更新日期：1989-09-01 00:00:00
abstract：:The steroidogenic activity of purified theca interstitial cells (TIC) from polycystic ovaries of a patient with virilizing congenital adrenal hyperplasia due to 21-hydroxylase deficiency was studied in serum-free medium. The results show that CVAH TIC have high intrinsic basal androgen secretory activity that is drama...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)60450-8
更新日期：1989-01-01 00:00:00
abstract：OBJECTIVE:To determine whether the oncofetal protein IMP3 is detectable in endometriomas with or without histological atypia and whether IMP3 staining can be used as a triage tool to identify foci of atypical endometriosis in doubtful cases. DESIGN:Retrospective study. SETTING:Academic department and referral center ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2013.02.002
更新日期：2013-06-01 00:00:00
abstract：OBJECTIVE:To investigate the possible role of phthalate, a ubiquitous chemical used in consumer products, in the pathogenesis of uterine leiomyoma. DESIGN:Experimental and prospective case-control study using human samples. SETTING:University hospital. PATIENT(S):Fifty-three women with histologic evidence of uterine...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2017.01.015
更新日期：2017-04-01 00:00:00
abstract：:Estrogen (E)/progestin therapy for functional ovarian cysts is widely used in clinical practice, but the efficacy of this treatment has not been determined in controlled trials. In this study, we examined the effect of E/progestin administration in a group of infertility patients enrolled in a program of ovulation ind...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章,随机对照试验
doi：
更新日期：1990-11-01 00:00:00
abstract：OBJECTIVE:To evaluate the effectiveness of growth hormone (GH) supplementation in improving the in vitro fertilization (IVF) outcomes of poor responders. DESIGN:Systematic review and meta-analysis. SETTING:Not applicable. PATIENT(S):Poor ovarian responders undergoing conventional IVF or intracytoplasmic sperm inject...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2020.03.007
更新日期：2020-07-01 00:00:00
abstract：:To evaluate the effect of luteal estrogen supplementation on pregnancy rates in normal responder IVF patients treated with recombinant FSH and fixed multidose GnRH antagonist, patients were randomized into two groups to receive vaginal 600 mg/d micronized progesterone for luteal phase supplementation with or without t...
journal_title：Fertility and sterility
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.fertnstert.2007.08.029
更新日期：2008-06-01 00:00:00
abstract：OBJECTIVE:To modify Tarkowski's air-dry technique for mouse oocytes to develop a rapid, consistent procedure for human oocytes that enables accurate scoring of meiotic stage. DESIGN, SETTING, AND PATIENTS:Meiotically immature human oocytes, obtained after oophorectomy, were cultured for various periods and then subjec...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)55021-3
更新日期：1992-05-01 00:00:00
abstract：:Evidence implicates the ovarian nerves in the regulation of hormonal and ovulatory functions. A technique for selectively denervating the in situ rabbit ovary is therefore of considerable investigative value. The ovarian artery was stripped of its adventitia and nerve bundles. Three weeks later, fluorescent histochemi...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1975-04-01 00:00:00
abstract：OBJECTIVE:To determine whether antisperm autoantibody production after prepubertal vas injury is influenced by immediate repair of the vas compared to delay of the reanastomosis until sexual maturity. DESIGN:Animal study comparing early repair, late repair, and sham-operated groups. SETTING:Research laboratory in a m...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(99)00501-4
更新日期：2000-02-01 00:00:00
abstract：:The transcervical division of uterine septa is usually described as a simple procedure associated with minimal morbidity. Two cases are presented describing late hemorrhage after this procedure. Approaches to avoiding this complication as well as management principles are described. ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)54984-x
更新日期：1992-04-01 00:00:00
abstract：:Transvaginal follicular aspiration (TVA) with ultrasonically guided needles allows the transfer of in vitro generated embryos to the fallopian tubes (TET), performing only one surgical procedure in the process. Up to now, this approach has been used to treat 16 couples with infertility due to severe male factor. Folli...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)60136-x
更新日期：1988-09-01 00:00:00
abstract：:A direct assay for measuring sperm-associated immunoglobulin G (IgG) and an indirect assay for measuring IgG plasma antisperm antibodies were devised utilizing fluorescein-labeled antihuman IgG and a fluorescein-activated flow cytometer. The method singularly analyzed Ig attached to the surface of sperm because sperm ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)48146-x
更新日期：1984-10-01 00:00:00
abstract：:The concentrations of pregnanediol-3-glucuronide (PGD) and pregnanolone (PN) were measured in daily morning urine specimens from 66 infertile women (40 with varying degrees of endometriosis and 26 control subjects) and correlated with daily changes in basal body temperature (BBT) and with midluteal levels of serum pro...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1983-11-01 00:00:00
abstract：OBJECTIVE:To analyze the potential role of extraovarian factors such as endometrium in the reproductive outcome of obese patients. DESIGN:Retrospective study. SETTING:University-affiliated infertility clinic, between January 2001 and July 2005. PATIENT(S):Women undergoing a total of 2656 first ovum donation (OD) cyc...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2006.11.162
更新日期：2007-08-01 00:00:00
abstract：:Surgical ablation of the inferior mesenteric plexus in the rat results in an excessive accumulation of sperm within the cauda epididymidis. The present study examined the effect of ablation of the inferior mesenteric plexus on the motility of cauda epididymal spermatozoa. One to 8 weeks after ablation of the inferior ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1990-06-01 00:00:00
abstract：OBJECTIVE:To determine whether polymorphisms in the estrogen receptor (ER) alpha and beta genes are associated with endometriosis in a Japanese population. DESIGN:Association study. SETTING:University hospital. PATIENT(S):Japanese women diagnosed with endometriosis by laparotomy or laparoscopy. INTERVENTION(S):Dete...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2004.02.094
更新日期：2004-06-01 00:00:00
abstract：OBJECTIVE:To evaluate whether stimulation of endometrium embryo transfer (SEET) can improve implantation rate and pregnancy rate (PR) for patients undergoing assisted reproductive technology (ART) for the first time by injecting embryo culture supernatant into the uterus before blastocyst transfer (BT). DESIGN:Randomi...
journal_title：Fertility and sterility
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.fertnstert.2008.08.076
更新日期：2009-10-01 00:00:00
abstract：OBJECTIVE:To evaluate sperm DNA fragmentation in normozoospermic male partners of couples undergoing infertility evaluation. DESIGN:Retrospective cohort study. SETTING:Clinical andrology laboratory. PATIENT(S):A total of 1,974 consecutive normozoospermic men selected from a larger cohort of 4,345 consecutive, nonazo...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2014.02.006
更新日期：2014-06-01 00:00:00
abstract：:One hundred infertile patients with laparoscopically confirmed ovarian endometriosis were treated with either intranasal (IN) Buserelin (Hoechst, AG, Frankfurt am Main, West Germany) (300 micrograms three times a day) or subcutaneous (SC) Buserelin implant (6.6 mg Buserelin). Serum estradiol was suppressed in the meno...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/s0015-0282(16)60783-5
更新日期：1989-07-01 00:00:00
abstract：:The author reports on 206 cases of endometriosis examined between 1970-1977. 172 patients had used OC (oral contraception) for a varying period of time prior to diagnosis of endometriosis; of these, 122 had taken only combined OCs containing 50 mg. or more of estrogen. These data neither support nor contradict the c...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)43887-2
更新日期：1979-03-01 00:00:00
abstract：:We found that four polymorphisms in the protein L-isoaspartyl-O-methyltransferase (PCMT1) gene, encoding a protein repair enzyme, are associated with premature ovarian failure (POF). All four polymorphisms were in strong linkage disequilibrium. The frequencies of two haplotypes were statistically significantly differe...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2008.03.078
更新日期：2009-04-01 00:00:00
abstract：OBJECTIVE:To investigate whether proteins secreted in urine differ between women with and without endometriosis. DESIGN:Laboratory study using human urine. SETTING:University-based laboratory. PATIENT(S):Women with and without endometriosis undergoing laparoscopy, hysteroscopy and curettage. INTERVENTION(S):Urine c...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2010.05.016
更新日期：2011-01-01 00:00:00
abstract：OBJECTIVE:To assess the endocrine and clinical responses to microdose GnRH agonist (GnRH-a) that was administered in the early follicular phase before controlled ovarian hyperstimulation to poor responders who were candidates for IVF-ET. DESIGN:Prospective nonrandomized trial with historical controls. SETTING:Tertiar...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(97)00575-x
更新日期：1998-03-01 00:00:00
abstract：STUDY OBJECTIVE:To determine the effect of baseline complex ovarian cysts on controlled ovarian hyperstimulation and in vitro fertilization (IVF) outcome. DESIGN:Retrospective analysis with stratification by stimulation regimen and the presence or absence of surgically documented endometriosis. PATIENTS:Two hundred s...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1992-06-01 00:00:00
abstract：OBJECTIVE:To assess right-left differences in ultrasonographic markers of ovarian morphology and determine the impact on the diagnosis of polycystic ovarian morphology (PCOM). DESIGN:A cross-sectional study of data collected from 2006 to 2018. SETTING:Academic clinical research centers. PATIENT(S):Women with polycys...
journal_title：Fertility and sterility
pub_type： 杂志文章,多中心研究
doi：10.1016/j.fertnstert.2019.06.016
更新日期：2019-11-01 00:00:00
abstract：OBJECTIVE:To investigate the effect of dienogest on the expression of Toll-like receptor (TLR) 4 in human endometrial epithelial cells. DESIGN:Prospective basic research study. SETTING:Pharmaceutical research center. PATIENT(S):None. INTERVENTION(S):Not applicable. MAIN OUTCOME MEASURE(S):TLR4 in the immortalized ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2011.09.040
更新日期：2011-12-01 00:00:00
abstract：OBJECTIVE:To measure serum anti-Müllerian hormone (AMH) during different phases of the menstrual cycle and to correlate the measurements with ovarian response and clinical-pregnancy rates in intracytoplasmic sperm injection cycles. DESIGN:Prospective cohort study. SETTING:University IVF unit. PATIENT(S):Thirty-three...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2007.05.040
更新日期：2008-06-01 00:00:00
abstract：:A laparotomy was performed in 40 rats and the cecum was subjected to a standardized trauma. Half the rats were treated with oxyphenbutazone in a dose of 40 mg/kg body weight daily for 14 days. The remaining 20 rats served as controls. All of the rats were killed 2 weeks after the laparotomy, and the number of intraper...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)42731-7
更新日期：1977-08-01 00:00:00
