Identification of antibody-laden sperm by cytofluorometry.

Abstract:

:A direct assay for measuring sperm-associated immunoglobulin G (IgG) and an indirect assay for measuring IgG plasma antisperm antibodies were devised utilizing fluorescein-labeled antihuman IgG and a fluorescein-activated flow cytometer. The method singularly analyzed Ig attached to the surface of sperm because sperm and nonsperm cells were separately assessed. The direct assay technique results correlated with results from a previously reported direct radiolabeled antiglobulin assay. As few as 100,000 sperm are required in the indirect method; the sensitivity of the indirect technique corresponded with that of a previously reported indirect radiolabeled antiglobulin assay.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Haas GG Jr,Cunningham ME

doi

10.1016/s0015-0282(16)48146-x

subject

Has Abstract

pub_date

1984-10-01 00:00:00

pages

606-13

issue

4

eissn

0015-0282

issn

1556-5653

pii

S0015-0282(16)48146-X

journal_volume

42

pub_type

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