Assessing autophagy in the context of photodynamic therapy.

abstract：:Autophagosome formation depends on a carefully orchestrated interplay between membrane-associated protein complexes. Initiation of macroautophagy/autophagy is mediated by the ULK1 (unc-51 like autophagy activating kinase 1) protein kinase complex and the autophagy-specific class III phosphatidylinositol 3-kinase compl...

journal_title：Autophagy

authors： Birgisdottir ÅB,Mouilleron S,Bhujabal Z,Wirth M,Sjøttem E,Evjen G,Zhang W,Lee R,O'Reilly N,Tooze SA,Lamark T,Johansen T

更新日期：2019-08-01 00:00:00

abstract：:In 2013, Dr. Lora Hooper and colleagues described the induction of antibacterial macroautophagy/autophagy in intestinal epithelial cells as a cytoprotective host defense mechanism against invading Salmonella enterica serovar Typhimurium (S. Typhimurium). Canonical autophagy functions in a primarily degradative capacit...

journal_title：Autophagy

authors： Delorme-Axford E,Klionsky DJ

更新日期：2018-01-01 00:00:00

abstract：:Ubiquitin-proteasome system and autophagy are the two major mechanisms for protein degradation in eukaryotic cells. LC3, a ubiquitin-like protein, plays an essential role in autophagy through its ability to be conjugated to phosphatidylethanolamine. In this study, we discovered a novel LC3-processing activity, and bio...

journal_title：Autophagy

authors： Gao Z,Gammoh N,Wong PM,Erdjument-Bromage H,Tempst P,Jiang X

更新日期：2010-01-01 00:00:00

abstract：:Single microRNA (miRNA) can regulate expression of several or multiple principal targets in a specific microenvironment. In different cellular contexts, the same miRNA may exhibit diverse functions, depending on the repertoire and stoichiometry of its direct mRNA targets. For instance, in breast cancer, microRNA-10b (...

journal_title：Autophagy

authors： Gabriely G,Teplyuk NM,Krichevsky AM

更新日期：2011-11-01 00:00:00

abstract：:To examine the functional significance and detailed morphological characteristics of starvation-induced autophagy in the adult heart, we starved green fluorescent protein (GFP)-microtubule-associated protein 1 light chain 3 (LC3) transgenic mice for up to 3 days. Electron microscopy revealed that, after as little as 1...

journal_title：Autophagy

authors： Takemura G,Kanamori H,Goto K,Maruyama R,Tsujimoto A,Fujiwara H,Seishima M,Minatoguchi S

更新日期：2009-10-01 00:00:00

abstract：:Phosphatidylinositol-3-phosphate (PtdIns3P) is essential for generating autophagosomes and regulating endocytic trafficking. Recently, we have shown that the activities of human PIK3C3/VPS34-containing complexes I and II, which synthesize PtdIns3P, are greatly affected by three membrane physicochemical parameters: lip...

journal_title：Autophagy

authors： Ohashi Y,Tremel S,Williams RL

更新日期：2021-01-15 00:00:00

abstract：:In yeast and mammals, selective vacuolar delivery and degradation of whole mitochondria, or mitophagy, represents an important quality control system and is achieved by a cargo recognition mechanism enabling selective elimination of dysfunctional mitochondria. As photosynthetic organelles that need light for energy pr...

journal_title：Autophagy

authors： Izumi M,Nakamura S

更新日期：2017-07-03 00:00:00

abstract：:Silver nanoparticles (Ag NPs) are cytotoxic to cancer cells and possess excellent potential as an antitumor agent. A variety of nanoparticles have been shown to induce autophagy, a critical cellular degradation process, and the elevated autophagy in most of these situations promotes cell death. Whether Ag NPs can indu...

journal_title：Autophagy

authors： Lin J,Huang Z,Wu H,Zhou W,Jin P,Wei P,Zhang Y,Zheng F,Zhang J,Xu J,Hu Y,Wang Y,Li Y,Gu N,Wen L

更新日期：2014-01-01 00:00:00

abstract：:Overcoming metabolic stress is a critical step in tumorigenesis. Acetyl coenzyme A (acetyl-CoA) converted from glucose or acetate is a substrate used for histone acetylation to regulate gene expression. However, how acetyl-CoA is produced under nutritional stress conditions is unclear. Herein we report that nutritiona...

journal_title：Autophagy

authors： Li X,Qian X,Lu Z

更新日期：2017-10-03 00:00:00

abstract：:Thioglycolate-elicited macrophages exhibit abundant conjugation of LC3 with PE (LC3-II). Among other autophagy-related (ATG) proteins, it is proposed that, like in yeast, both ATG5 and ATG7 are essential for LC3 conjugation. Using atg5-deficient ( -/- ) and atg7-/- macroph...

journal_title：Autophagy

authors： Vujić N,Bradić I,Goeritzer M,Kuentzel KB,Rainer S,Kratky D,Radović B

更新日期：2021-01-18 00:00:00

abstract：:Autophagy is a highly conserved homeostatic pathway that plays an important role in tumor development and progression by acting on cancer cells in a cell-autonomous mechanism. However, the solid tumor is not an island, but rather an ensemble performance that includes nonmalignant stromal cells, such as macrophages. A ...

journal_title：Autophagy

authors： Chen P,Cescon M,Bonaldo P

更新日期：2014-02-01 00:00:00

abstract：:The precise degradation of dysfunctional mitochondria by mitophagy is essential for maintaining neuronal homeostasis. HTT (huntingtin) can interact with numerous other proteins and thereby perform multiple biological functions within the cell. In this study, we investigated the role of HTT during mitophagy and analyze...

journal_title：Autophagy

authors： Franco-Iborra S,Plaza-Zabala A,Montpeyo M,Sebastian D,Vila M,Martinez-Vicente M

更新日期：2020-02-24 00:00:00

abstract：:Autophagy is a highly conserved intracellular degradation system, and recently was shown to display circadian rhythms in mice. The mechanisms underlying circadian regulation of autophagy, however, are still unclear. Here, we observed that numbers of autophagosomes and autolysosomes exhibit daily rhythms in the zebrafi...

journal_title：Autophagy

authors： Huang G,Zhang F,Ye Q,Wang H

更新日期：2016-08-02 00:00:00

abstract：:In the fungal pathogen Fusarium oxysporum, vegetative hyphal fusion triggers nuclear mitotic division in the invading hypha followed by migration of a nucleus into the receptor hypha and degradation of the resident nucleus. Here we examined the role of autophagy in fusion-induced nuclear degradation. A search of the F...

journal_title：Autophagy

authors： Corral-Ramos C,Roca MG,Di Pietro A,Roncero MI,Ruiz-Roldán C

更新日期：2015-01-01 00:00:00

abstract：:The conserved target of rapamycin (TOR) kinase is a central regulator of cell growth in response to nutrient availability. TOR forms 2 structurally and functionally distinct complexes, TORC1 and TORC2, and negatively regulates autophagy via TORC1. Here we demonstrate TOR also operates independently through the TORC2 s...

journal_title：Autophagy

authors： Vlahakis A,Powers T

更新日期：2014-01-01 00:00:00

abstract：:The accumulation of ubiquitin-positive protein aggregates has been implicated in the pathogenesis of neurodegenerative diseases, heart disease and diabetes. Emerging evidence indicates that the autophagy lysosomal pathway plays a critical role in the clearance of ubiquitin aggregates, a process that is mediated by the...

journal_title：Autophagy

authors： Fan W,Tang Z,Chen D,Moughon D,Ding X,Chen S,Zhu M,Zhong Q

更新日期：2010-07-01 00:00:00

abstract：:Some key questions being examined in the field of autophagy concern the origin of the membrane that forms the sequestering vesicle, the function of the related machinery, including the identification of new components and binding partners of previously identified autophagy-related proteins and the mechanism of autopha...

journal_title：Autophagy

authors： Vellai T,Klionsky DJ

更新日期：2010-01-01 00:00:00

abstract：:Lysosomal storage diseases (LSDs) are debilitating genetic conditions that frequently manifest as neurodegenerative disorders. They severely affect eye, motor and cognitive functions and, in most cases, abbreviate the lifespan. Postmitotic cells such as neurons and mononuclear phagocytes rich in lysosomes are most oft...

journal_title：Autophagy

authors： Kiselyov K,Jennigs JJ Jr,Rbaibi Y,Chu CT

更新日期：2007-05-01 00:00:00

abstract：:Mechanisms to protect against viral infections are crucial during pregnancy as maternal-fetal transmission can have serious pathological outcomes, including fetal infection and its sequelae, such as growth restriction, birth defects, and/or fetal death. The trophoblast forms the interface between the feto-placental un...

journal_title：Autophagy

authors： Delorme-Axford E,Bayer A,Sadovsky Y,Coyne CB

更新日期：2013-12-01 00:00:00

abstract：:Chronic drinking leads to myocardial contractile dysfunction where ethanol metabolism plays an essential role. Acetaldehyde, the main ethanol metabolite, mediates alcohol-induced cell injury although the underlying mechanism is still elusive. This study was designed to examine the mechanism involved in accelerated eth...

journal_title：Autophagy

authors： Guo R,Hu N,Kandadi MR,Ren J

更新日期：2012-04-01 00:00:00

abstract：:Cargo-based assays have proven invaluable in the study of macroautophagy in yeast and mammalian cells. Proteomic analysis of autolysosomes identified the metabolic enzyme, betaine homocysteine methyltransferase (BHMT), as a potential cargo-based, end-point marker for mammalian macroautophagy. To test whether degradati...

journal_title：Autophagy

authors： Mercer CA,Kaliappan A,Dennis PB

更新日期：2008-02-01 00:00:00

abstract：:SNCA/α-synuclein aggregation plays a crucial role in synucleinopathies such as Parkinson disease and dementia with Lewy bodies. Aggregating and nonaggregating SNCA species are degraded by the autophagy-lysosomal pathway (ALP). Previously, we have shown that the ALP is not only responsible for SNCA degradation but is a...

journal_title：Autophagy

authors： Poehler AM,Xiang W,Spitzer P,May VE,Meixner H,Rockenstein E,Chutna O,Outeiro TF,Winkler J,Masliah E,Klucken J

更新日期：2014-01-01 00:00:00

abstract：:This Editor's Corner may sound like the title of a mystery novel, but it actually reflects a question I have about the puncta articles that appear in Autophagy (or rather, the ones that do not appear). In particular, I am surprised by the number of solicitations sent out for puncta that are either ignored, or, less fr...

journal_title：Autophagy

authors： Klionsky DJ

更新日期：2017-09-02 00:00:00

abstract：:The Syrian hamster Harderian gland (HG) is a juxtaorbital organ exhibiting marked gender-associated morphological differences. Regarding contents of porphyrins, this gland is a good model for studying physiological oxidative stress effects, since both sexes present strong (in females) and moderate (in males) levels of...

journal_title：Autophagy

authors： Coto-Montes A,Tomás-Zapico C

更新日期：2006-04-01 00:00:00

abstract：:Autophagy research continues to expand exponentially. Clearly autophagy and metabolism are intimately connected; however, the rapid expansion of research into this topic inevitably brings the risk that important basic knowledge of metabolism will be overlooked when considering experimental data. Unfortunately, unaware...

journal_title：Autophagy

authors： Meijer AJ

更新日期：2009-01-01 00:00:00

abstract：:Multiple stress pathways result in the induction of autophagy and apoptosis. Current methods (e.g., protein gel blot, microscopy) do not offer quantitative single-cell resolution, thus making it difficult to discern if these pathways are mutually exclusive or, in some situations, cooperative in executing cell death. W...

journal_title：Autophagy

authors： de la Calle C,Joubert PE,Law HK,Hasan M,Albert ML

更新日期：2011-09-01 00:00:00

abstract：:We demonstrated for the first time that the COP9 signalosome (COPS) controls the degradation of a surrogate and a bona fide misfolded protein in the cytosol of cardiomyocytes likely via supporting ubiquitination by CUL/cullin-RING ligases, and that Cops8 hypomorphism exacerbates cardiac proteinopathy in mice, in which...

journal_title：Autophagy

authors： Liu J,Su H,Wang X

更新日期：2016-01-01 00:00:00

abstract：:Autophagy plays critical roles in plant responses to stress. In contrast to the wealth of information concerning the core process of plant autophagosome assembly, our understanding of the regulation of autophagy is limited. In this study, we demonstrated that transcription factor HsfA1a played a critical role in tomat...

journal_title：Autophagy

authors： Wang Y,Cai S,Yin L,Shi K,Xia X,Zhou Y,Yu J,Zhou J

更新日期：2015-11-02 00:00:00

abstract：:We detail here a protocol using tandem-tagged mCherry-EGFP-LC3 (C-G-LC3) to quantify autophagic flux in single cells by ratiometric flow cytometry and to isolate subpopulations of cells based on their relative levels of autophagic flux. This robust and sensitive method measures autophagic flux rather than autophagosom...

journal_title：Autophagy

authors： Gump JM,Thorburn A

更新日期：2014-07-01 00:00:00

abstract：:Neuroblastoma is the most common extracranial solid tumor in childhood. Despite intense multimodal therapy and many improvements through basic scientific and clinical research, the successful response of advanced-stage patients to chemotherapy remains poor. Autophagy is a cytoprotective mechanism that may help advance...

journal_title：Autophagy

authors： Oehme I,Lodrini M,Brady NR,Witt O

更新日期：2013-12-01 00:00:00