Abstract:
:Estrogens have been associated with risk for epithelial ovarian cancer (OVCA). Both IL-6 and IL-8 are also likely involved in the progression of OVCA. In order to discover the underline molecular mechanism, we investigated the modulation of estrogen and two cytokines in the growth and progression of epithelial OVCA. In these studies, the effect of 17beta-estradiol (E(2)) on the expression levels of IL-6, IL-8 and their receptors was investigated. The effect of IL-6 and IL-8 on activation of estrogen-responsive promoter as well as estrogen receptor (ER)alpha and ER beta expression was also analyzed. Gene expression profile analysis revealed that CAOV-3 and OVCAR-3 cells, which express ER, IL-6 and IL-8 receptors, are suitable model for this study. We found that E(2) not only enhanced IL-6 and IL-8 production via NF-kappaB signaling pathway, but also modulated their respective receptor expression. Tamoxifen (Txf), an ER antagonist, completely abolished E(2)-stimulated cell growth and the expression of IL-6 and IL-8. IL-6/IL-8-induced cell proliferation was completely blocked by their specific neutralizing antibodies, which partially inhibited E(2)-induced cell growth. In the absence of estrogen, both cytokines activated estrogen-responsive promoter, which was completely blocked by Txf, and caused a dose-dependent ER alpha increase and ER beta decrease. Pretreatment of OVCAR-3 with p38 MAPK, MEK1/2 or ErbB2 MAPK inhibitors, respectively, blocked IL-6-mediated induction of estrogen-responsive promoter while Src inhibitor blocked IL-8-induced activation of estrogen-responsive promoter. These results provide a novel mechanism that estrogens, IL-6 and IL-8 may form a common amplifying signaling cascade to modulate OVCA growth and progression. Estrogen-induced OVCA proliferation is partially occurring via enhanced IL-6 and IL-8 production and modulated their receptors, and IL-6/IL-8 could also promote OVCA growth through an ER alpha pathway.
journal_name
Cytokinejournal_title
Cytokineauthors
Yang J,Wang Y,Gao Y,Shao J,Zhang XJ,Yao Zdoi
10.1016/j.cyto.2009.03.013subject
Has Abstractpub_date
2009-06-01 00:00:00pages
382-91issue
3eissn
1043-4666issn
1096-0023pii
S1043-4666(09)00101-Xjournal_volume
46pub_type
杂志文章相关文献
CYTOKINE文献大全abstract:BACKGROUND:Vascular endothelial growth factor (VEGF) is the key regulator of angiogenesis in the development of various cancers. Previous studies have examined the relationship between VEGF gene promoter polymorphisms such as -2578C/A and -460C/T and bladder cancer risk; however, these results are inconclusive. Therefo...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2020.155112
更新日期:2020-07-01 00:00:00
abstract::D-dopachrome tautomerase (D-DT) is a newly described cytokine and a member of the macrophage migration inhibitory factor (MIF) protein superfamily. MIF is a broadly expressed pro-inflammatory cytokine that regulates both the innate and the adaptive immune response. MIF activates the MAP kinase cascade, modulates cell ...
journal_title:Cytokine
pub_type: 杂志文章,评审
doi:10.1016/j.cyto.2012.03.014
更新日期:2012-07-01 00:00:00
abstract::Tenidap is a novel anti-inflammatory and anti-arthritic agent that lowers intracellular pH and suppresses anion transport when applied to cells in vitro. Both of these parameters are known to influence pro-inflammatory cell function. To investigate whether tenidap can modulate cellular responses to cytokine stimulatio...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1006/cyto.1995.1027
更新日期:1995-02-01 00:00:00
abstract::Three representative cytokines interleukin (IL-8), RANTES and tumour necrosis factor alpha (TNF-alpha) have a concentration-dependent chemotactic effect on the unicellular Tetrahymena. Maximal effective concentrations of IL-8 (1 ng/ml) and RANTES (75 ng/ml) are in the same range as in mammals, which indicates an evolu...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1006/cyto.1997.0328
更新日期:1998-07-01 00:00:00
abstract::We had earlier shown that human foetal epithelial cells (WISH), growth-inhibited by interferon gamma (IFNgamma), were reversibly detained at a point prior to DNA synthesis. In the present study, we determined the window of action of IFNgamma in the G1 phase duration and the exact point of detention of WISH cells in ce...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2007.02.013
更新日期:2007-02-01 00:00:00
abstract::Inflammatory processes are implicated in gastric cancer development. In contrast, the role of inflammation and proinflammatory cytokines in established cancer remains to be clarified. We investigated the contribution of IL-17A versus IL-17F-mediated intracellular signalling pathways in human gastric adenocarcinoma AGS...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2007.06.002
更新日期:2007-06-01 00:00:00
abstract:PURPOSE:Tumor necrosis factor (TNFα) is a proinflammatory cytokine and has been a target for intervention in human sepsis. However, inhibition of TNF-α with a high dose of a TNF-receptor fusion protein in patients with septic shock worsened patient survival. This study was designed to investigate whether blocking TNF-α...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2013.04.002
更新日期:2013-07-01 00:00:00
abstract::Since its first description in 1966, macrophage migration inhibitory factor (MIF) was found to play a critical role in inflammatory and immune responses as well as in disease pathogenesis especially in tumor pathogenesis and cancer progression. MIF is expressed in different cell types and is associated with many disea...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2017.06.006
更新日期:2017-09-01 00:00:00
abstract::Azidothymidine (AZT)-induced anemia in mice can be reversed by the administration of IGF-IL-3 (fusion protein of insulin-like growth factor II (IGF II) and interleukin 3). Although interleukin 3 (IL-3) and erythropoietin (EPO) are known to act synergistically on hematopoietic cell proliferation in vitro, injection of ...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1006/cyto.2002.1029
更新日期:2002-04-07 00:00:00
abstract::LIGHT (lymphotoxin-like inducible protein that competes with glycoprotein D for binding herpesvirus entry mediator on T cells) is a recently identified of the tumor necrosis factor alpha (TNF-alpha) ligand superfamily. We wanted to establish whether the presence of chronic viral hepatitis could be implicated in enhanc...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2007.07.189
更新日期:2007-09-01 00:00:00
abstract::Treatment of non-small cell lung carcinoma (NSCLC) remains at a disappointingly low success rate. Not only is metastatic spread common in NSCLC, but therapeutic success decreases dramatically once metastases are present. Understanding factors which contribute to poor prognosis in NSCLC is critical for development of m...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2009.11.015
更新日期:2010-03-01 00:00:00
abstract::Natural terrestrial ionizing radiations emerge from uranium deposits and can impact human tissues by affecting DNA bases which constitute genes. Human interferon alpha-2b (hIFNα-2b) gene synthesizes a protein which exhibits anticancerous, immunomodulatory, anti-proliferative and antiviral properties. This research aim...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2015.05.011
更新日期:2015-12-01 00:00:00
abstract::Interleukin 1 (IL-1) activates p42/p44 and p38 mitogen-activated protein kinases (MAP kinases) in target cells. Here we have used two specific inhibitors, PD98059 which inhibits MAP kinase kinase (MEK), and SB203580 which inhibits p38 MAP kinase to explore the involvement of these kinases in the induction of IL-2 by I...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1006/cyto.1998.0478
更新日期:1999-09-01 00:00:00
abstract::The purpose of the study was to reassess the utility of transforming growth factor-beta-1 (TGF-beta1) together with dosimetric and tumor parameters as a predictor for radiation pneumonitis (RP). Of the 121 patients studied, 32 (26.4%) developed grade > or =1 RP, and 27 (22.3%) developed grade > or =2 RP. For the endpo...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2006.07.021
更新日期:2006-08-01 00:00:00
abstract:BACKGROUND:Why severity varies in blastomycosis outbreaks remains unresolved. In experimental pulmonary blastomycosis, susceptibility varied in mouse strains. In susceptible BALB/c the response is Th-2, in immunized, resistance is associated with Th-1. Can susceptibility be redirected by IL-12? Methods, results: BALB/c...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2006.08.004
更新日期:2006-09-01 00:00:00
abstract::The supernatant from normal liver cell (Clone 1469) culture enhanced polyclonal antibody production in mouse spleen cells but not in human peripheral blood lymphocytes. The supernatants from transforming growth factor beta (TGF-beta)-treated C3H/HeN spleen cells augmented greatly polyclonal antibody responses compared...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1006/cyto.1996.0112
更新日期:1996-11-01 00:00:00
abstract:BACKGROUND:Resistin is a novel cysteine-rich protein that plays a role in the development of insulin resistance and atherosclerosis in rodents, while its role in humans is unclear. C-reactive protein (CRP) is an important risk predictor for coronary heart disease, and it can also modify the expression of genes involved...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2007.09.011
更新日期:2007-12-01 00:00:00
abstract::We have cloned and performed the first functional characterization of the chemokine receptor, CCR3, of Cynomolgus monkey (Macaca fascicularis). The deduced amino acid sequence of the cloned Cynomolgus CCR3 was found to be more similar to that of a previously-reported Rhesus (Macaca mulatta) CCR3 (99.4%) than that of a...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1006/cyto.2002.1971
更新日期:2002-09-21 00:00:00
abstract:BACKGROUND:Cutaneous melanoma displays high morbidity and mortality rates. Isolated limb perfusion with melphalan (Mel) is used for the treatment of non-resectable, locally advanced extremity melanomas. When combined with tumor necrosis factor alpha (TNF-alpha) treatment, the complete response varies between 70% and 90...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2013.02.022
更新日期:2013-05-01 00:00:00
abstract::Therapeutic plasmapheresis is a recognized medical procedure in which various techniques are used to separate and remove undesirable or excessively elevated plasma elements from blood. The main purpose of the procedure is to remove the substances responsible for the disease (autoantibodies, circulating immune complexe...
journal_title:Cytokine
pub_type: 杂志文章,评审
doi:10.1016/j.cyto.2011.08.038
更新日期:2011-12-01 00:00:00
abstract::Systemic sclerosis (SSc) has the highest fatality rate among connective tissue diseases and is characterized by vascular damage, inflammation and fibrosis. Currently, no therapy has proven effective in modifying the course of SSc, a reflection of its complex pathogenesis. T cell-derived cytokines have been implicated ...
journal_title:Cytokine
pub_type: 杂志文章,评审
doi:10.1016/j.cyto.2011.08.030
更新日期:2011-12-01 00:00:00
abstract::The purpose of this study is to evaluate local levels of interleukin-1 beta (IL-1 beta), -4 (IL-4), -6 (IL-6), and tumour necrosis factor-alpha (TNF-alpha), in a model of murine osteomyelitis due to Staphylococcus aureus. Cytokine levels in supernatants derived from bone homogenates were determined by enzyme-linked im...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1006/cyto.2002.1039
更新日期:2002-07-21 00:00:00
abstract::Oncostatin M (OSM) and leukaemia inhibitory factor (LIF) exhibit pleiotropic biological activities and share many structural and genetic features. The two cytokines bind with high affinity to the same receptor (LIF/OSM receptor), which consists of the LIF receptor alpha chain (LIFRalpha) and the signal transduction un...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1006/cyto.1999.0550
更新日期:2000-02-01 00:00:00
abstract::Non-Hodgkin's lymphomas (NHL) constitute a heterogenous group of mainly B-cell lymphoproliferative diseases with different patterns of clinical behaviour. Biological mechanisms leading to development of NHL are not clearly understood. Transforming growth factor-beta1 (TGF-beta1) influences B cell growth and developmen...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2005.12.010
更新日期:2006-02-07 00:00:00
abstract::iNKT cells, CD1d dependent natural killer T cells are a unique population of T cells. The capacity of iNKT cells to produce regulatory cytokines first provided an indication of their regulatory potential. Later on, in experimental models as well as in patients afflicted with an auto-immune disease, such as Type 1 diab...
journal_title:Cytokine
pub_type: 杂志文章,评审
doi:10.1016/j.cyto.2010.11.001
更新日期:2011-03-01 00:00:00
abstract::The protective effect of interleukin 1 alpha (IL-1 alpha) in mice with acetaminophen (AAP)-induced hepatitis was investigated. IL-1 alpha had a significant protective effect if given 2 or more hours (up to 24 hours) before AAP; it significantly reduced mortality of mice and decreased serum transaminase level. The maxi...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/1043-4666(93)90004-o
更新日期:1993-05-01 00:00:00
abstract::The response of the fetal rat Type II pneumocyte (FTIIP), the stem cell of the alveolar epithelium, to hyperoxia would be helpful to understand the effects of oxygen-induced injury to the developing lung. Our goals were to evaluate the effect of antioxidants (AO) on apoptosis and release of cytokines in freshly isolat...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2004.06.001
更新日期:2004-10-07 00:00:00
abstract::Interferon (IFN)-alpha and -beta are important antiviral mediators. IFN-alpha is widely used for the treatment of chronic hepatitis B. In our previous studies, a subtype of woodchuck IFN-alpha (wIFN-alpha) was characterized and has been shown to be active in suppressing the replication of woodchuck hepatitis virus (WH...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2007.11.002
更新日期:2008-02-01 00:00:00
abstract:BACKGROUND:Although transforming growth factor-beta (TGF-beta), a growth regulator of hepatocytes, induces cell death under pathological conditions, responsiveness of hepatocytes to hypoxic stimulus has not been fully defined. This study aimed at investigating the role of TGF-beta1 in hypoxia-induced hepatotoxicity usi...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2009.03.004
更新日期:2009-07-01 00:00:00
abstract::The history of the discovery and the development of our knowledge of IL-4 exemplifies the path of progress in biomedical science. There are unanticipated twists and turns although progress is made, sometimes quickly, other times far too slowly. Illustrative is the extended time from the first report of IL-4 in 1982 to...
journal_title:Cytokine
pub_type: 杂志文章,评审
doi:10.1016/j.cyto.2015.01.038
更新日期:2015-09-01 00:00:00