Abstract:
:Primary fibroblasts initiated from skin biopsies of Wistar rats were transfected with a plasmid that encodes the human growth hormone and the neomycin resistance genes. Cell clones selected for G418 resistance and expressing high levels of human growth hormone were propagated in vitro and subsequently transplanted into the cerebral cortex of adult allogeneic rats. Grafts were examined by immunocytochemistry at weekly intervals up to 2 months. Fibroblasts in the transplants survived and expressed growth hormone clearly up to 4 weeks, but showed reduced expression at 6 to 8 weeks. Significant levels of human growth hormone were also detected by radioimmunoassay in the serum of the host rats up to 1 month. The experiments demonstrate that primary fibroblasts can be genetically modified to deliver a new gene product into the central nervous system, and the gene product can pass the blood-brain barrier to enter the systemic circulation. This model illustrates the potential to introduce desired products into the brain through the genetic alteration of autologous primary fibroblasts.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Doering LC,Chang PLdoi
10.1002/jnr.490290304subject
Has Abstractpub_date
1991-07-01 00:00:00pages
292-8issue
3eissn
0360-4012issn
1097-4547journal_volume
29pub_type
杂志文章abstract::Isotonic media containing sodium salts from weak organic acids induce cell swelling in several experimental preparations (Grinstein et al., 1984; Jakubovicz et al., 1987). In vivo perfusion of rat dentate gyrus, using a microdialysis probe, with modified Krebs-Ringer bicarbonate solutions in which 50 mM NaCl was isoto...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490260205
更新日期:1990-06-01 00:00:00
abstract::The five major antioxidant enzymes, glutathione, and in vivo or in vitro stimulated (Fe(++)-ascorbate) peroxidation were similar in old and young Rana perezi frogs. Long-term (2.5 months) treatment with aminotriazole strongly decreased cerebral catalase (CAT) activity and increased in vivo but not in vitro peroxidatio...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490260314
更新日期:1990-07-01 00:00:00
abstract::The brainstem nucleus of the solitary tract (NTS) is the key integrating relay in the central processing of sensory information from the thoracic and from most subdiaphragmatic viscera. Modulation of neuronal excitability and synaptic activity in the NTS by nicotinic agents can have potent effects on vital physiologic...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23436
更新日期:2014-11-01 00:00:00
abstract::The G protein-coupled muscarinic acetylcholine receptor (mAChR) isoforms have been identified in neural stem/progenitor (or precursor) cells. In previous studies, activation of these receptors induced elevations in intracellular Ca(2+) signals and mitogen-activated protein (MAP) kinase activity that led to enhanced DN...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10591
更新日期:2003-05-01 00:00:00
abstract::The role of the transcription factor AP-1 in regulating D2 receptor transcriptional activity was investigated in D2 receptor expressing neuroblastoma cells, NB41A3, and in non-D2 receptor expressing CHO cells. Deletion of a region containing the putative AP-1 binding site resulted in a significant reduction in the act...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19971001)50:1<23::AID-JNR3
更新日期:1997-10-01 00:00:00
abstract::The effects of glutamate on intracellular free Ca2+, [Ca2+]i, and neurotoxicity were compared in cerebellar granule neurons in vitro. [Ca2+]i was measured with fura-2 and digital fluorescence imaging microscopy; neurotoxicity was monitored using a vital dye and colorimetric analysis. Glutamate produced dose-dependent ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490280317
更新日期:1991-03-01 00:00:00
abstract::Astrocytes are the most abundant glial cell in the central nervous system. They modulate synaptic function through a variety of mechanisms, and yet remain relatively understudied with respect to overall neuronal circuit function. Exploiting the tractability of the mouse olfactory system, we manipulated astrocyte activ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.24634
更新日期:2020-08-01 00:00:00
abstract::An aminopeptidase purified to homogeneity from cytosol of rat brain cleaved dynorphins having 5-17 residues and selected proenkephalins at the Tyr-Gly bond only to release Tyr and the desTyr fragments. The enzyme protein consisted of a single polypeptide chain of Mr 103,000 and was inhibited by puromycin, bestatin, an...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490110311
更新日期:1984-01-01 00:00:00
abstract::Structural microtubule-associated proteins (MAPs) interact with microtubules to regulate the various dynamic stages of microtubules. The purpose of this study was to measure the impact of myc-tagged MAP1a fragments on microtubule dynamic phases in vivo. Cells from an epithelial kidney cell line (LLCPK1) that had been ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21920
更新日期:2009-04-01 00:00:00
abstract::Thirty-day readmission rates has been increasingly used by clinicians, hospital administrators, and policy makers as a metric for the quality of care. However, the 30-day readmission rates may be affected by other factors intrinsic to the patient and may not be a good measure of the quality of care provided by the hos...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.24398
更新日期:2020-01-01 00:00:00
abstract::During surgical intervention in medically refractory temporal lobe epilepsy (TLE) patients, diagnosed with either mesial temporal lobe sclerosis (MTS)- or tumor (T)-associated TLE, biopsies were taken from the anterior temporal neocortex and the hippocampal region. Synaptosomes, isolated from these biopsies were used ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(20000601)60:5<686::AID-JNR
更新日期:2000-06-01 00:00:00
abstract::Raf kinases are downstream effectors of Ras and upstream activators of the MEK-ERK cascade. Ras and MEK-ERK signaling play roles in learning and memory (L&M) and neural plasticity, but the roles of Raf kinases in L&M and plasticity are unclear. Among Raf isoforms, B-raf is preferentially expressed in the brain. To det...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20703
更新日期:2006-01-01 00:00:00
abstract::Gamma-Aminobutyric acid (GABA) is the principal inhibitory neurotransmitter in the brain. A deficiency of GABAergic inhibition mediated via the GABAA receptor complex has for a long time been suspected to be a central factor in epileptogenesis. Status epilepticus is a condition of sustained and prolonged excitation of...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19981215)54:6<863::AID-JNR
更新日期:1998-12-15 00:00:00
abstract::Changes in executive function are at the root of most cognitive problems associated with Parkinson's disease. Because dopaminergic treatment does not necessarily alleviate deficits in executive function, it has been hypothesized that dysfunction of neurotransmitters/systems other than dopamine (DA) may be associated w...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23638
更新日期:2015-12-01 00:00:00
abstract::Among the ion channels and pumps activated by growth factor stimulation, K+ channels have been implicated in the growth and proliferation of several cancer cell lines. The role of these channels in central nervous system tumors, however, has not been described. This study used the malignant astrocytoma cell lines U87 ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:
更新日期:1997-04-15 00:00:00
abstract::The multifactorial nature of traumatic brain injury (TBI), especially the complex secondary tissue injury involving intertwined networks of molecular pathways that mediate cellular behavior, has confounded attempts to elucidate the pathology underlying the progression of TBI. Here, systems biology strategies are explo...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23503
更新日期:2015-02-01 00:00:00
abstract::Manganese (Mn) is an essential metal and plays an important role in the brain. To evaluate Mn uptake into the brain during development and aging, 54Mn concentrations in the brain of rats aged from 5 days to 95 weeks were measured after injection of 54MnCl2. 54Mn concentration in the brain of 5-day-old rats was the hig...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19990401)56:1<93::AID-JNR1
更新日期:1999-04-01 00:00:00
abstract::Neuroepithelial b2T cells were derived from the hindbrain and the spinal cord of mouse transgenic embryos, which expressed SV40 T antigen under the control of a Hoxb2 enhancer. Strikingly, b2T cell lines of either origin exhibit a very similar gene expression pattern, including markers of the hindbrain and the spinal ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10716
更新日期:2003-09-15 00:00:00
abstract::Recent experiments have shown that Arg, Lys, and Leu can be incorporated posttranslationally into proteins of regenerating sciatic nerves of rats. The present experiments investigate a mixture of 15 radioactive amino acids to determine if additional amino acids can be conjugated posttranslationally to proteins of rege...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490250407
更新日期:1990-04-01 00:00:00
abstract::An early and essential step in the formation of functional neuronal circuits is the establishment of cell polarity, a process involving the morphological and functional differentiation of the axon (axonogenesis). We now report that treatment of cultured embryonic hippocampal neurons with ethidium bromide (EtBr; an age...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19990401)56:1<8::AID-JNR2>
更新日期:1999-04-01 00:00:00
abstract::DBA/2J mice are one of several animal strains used for experimental models of both intraocular hypertension and glaucoma. This study investigates the relationship between endoplasmic reticulum (ER) stress and optic nerve degeneration in DBA/2J mice. Intraocular pressure (IOP) was measured in DBA/2J mice between the ag...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23630
更新日期:2015-11-01 00:00:00
abstract::We have developed an animal model of learning and memory impairment associated with activation of microglia in the mouse brain. Injection of lipopolysaccharide into the CA1 region of the mouse hippocampus resulted in an increased production of inflammatory cytokines, such as interleukin-1β. Immunostaining for interleu...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.22582
更新日期:2011-04-01 00:00:00
abstract::Niemann-Pick C (NPC) disease is an autosomal recessive lipidosis characterized by lysosomal accumulations of unesterified cholesterol. Cultured NPC cells exhibit a defect in the intracellular trafficking of LDL-derived cholesterol that leads to lysosomal accumulations of unesterified cholesterol. We found in a prelimi...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(sici)1097-4547(19970801)49:3<389::aid-jnr
更新日期:1997-08-01 00:00:00
abstract::Remyelination of the central nervous system in multiple sclerosis patients is often incomplete. Remyelination depends on normal oligodendrogenesis and the differentiation of oligodendrocyte precursor cells (OPC) into mature oligodendrocytes (OL). Inhibitor of DNA binding (ID), a transcription factor, is thought to inh...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.22826
更新日期:2012-05-01 00:00:00
abstract::Although proteins belonging to the sHSP superfamily are diverse in sequence and size, most share characteristic features, including 1) a small molecular mass of 12-43 kDa, 2) a conserved alpha-crystallin domain of 80-100 residues, 3) formation of large oligomers, 4) a dynamic quaternary structure, and 5) induction by ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.21682
更新日期:2008-08-01 00:00:00
abstract::The expression of the immunoglobulin superfamily member myelin-associated glycoprotein (MAG) and the extracellular matrix glycoprotein tenascin-R (TN-R) by oligodendrocytes overlaps in time and space. The two molecules can be neurite outgrowth-inhibitory or -promoting depending on the neuronal cell type and the enviro...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19990315)55:6<687::AID-JNR
更新日期:1999-03-15 00:00:00
abstract::The mechanisms by which polyglutamine expansion causes common features of neuronal death remain unclear. Here we describe an approach for delivering polyglutamine expansions directly into cultured sympathetic neurons. Glutamine (Q) residues (n = 10, 22, 30) were conjugated with a peptide possessing translocation prope...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20461
更新日期:2005-05-15 00:00:00
abstract::Pigment epithelium-derived factor (PEDF) is a neurotrophic protein synthesized and secreted by retinal pigment epithelial (RPE) cells in early embryogenesis and has been shown to be present in the extracellular matrix between the RPE cells and the neural retina. It induces neuronal differentiation and promotes surviva...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:
更新日期:1999-09-15 00:00:00
abstract::Lipoic acid plays a crucial role as antioxidant and metabolic component of enzymes involved in glucose metabolism of different cell types. Choline alphoscerate (α-glyceryl-phosphoryl-choline [αGPC]) is a semisynthetic derivative of phosphatidylcholines representing, among acetilcholine precursors, a cholinergic drug. ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23289
更新日期:2014-01-01 00:00:00
abstract::The most visible and, until very recently, the only hypothesis regarding the involvement of microglial cells in Alzheimer's disease (AD) pathogenesis is centered around the notion that activated microglia are neurotoxin-producing immune effector cells actively involved in causing the neurodegeneration that is the caus...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.20093
更新日期:2004-07-01 00:00:00