Abstract:
:Numerous dietary phytochemicals have shown anti-breast carcinogenic activities when tested in vitro; however, in most cases, the demonstrated efficacy of individual phytochemicals requires doses not readily achievable in vivo. Therefore, whether diets might exert translational promises and benefits in clinical settings and prevention of breast cancer remain unclear. Since cancer cells are endowed with complex, redundant, converging and diverging pathways spanning both the genetic and metabolic networks that are not merely replicates of those in normal cells, it is of interest to test whether a multicomponent approach involving lower, physiologically relevant doses of natural dietary agents may be developed as a chemopreventive strategy for breast cancer. Herein, we investigated, using the estrogen receptor-positive MCF-7 breast cancer cells as a model, whether the combination of epigallocatechin gallate (EGCG), resveratrol and gamma-tocotrienol at suboptimal doses elicits synergism in suppressing cell proliferation, modulating gene expression, and increasing antioxidant activity, as compared to each of the three phytochemicals added alone. The results showed that there was a approximately 33, 50 and 58% inhibition of cell proliferation by > or =50 microM EGCG, > or =25 microM resveratrol and > or =10 microM gamma-tocotrienol, respectively, added as a single agent. When a suboptimal dose (10 microM) of each phytochemical was used, a significant additive effect in suppression of cell proliferation was observed with the combination of resveratrol and gamma-tocotrienol whereas the three phytochemicals added together did not produce more pronounced inhibition of cell proliferation. A significant additive effect in reducing cyclin D1 and bcl-2 expression was found when gamma-tocotrienol was added with either EGCG or resveratrol. Functional synergism among the three phytochemicals was only observed in the induction of quinone reductase NQO1. These results suggest that diet-based protection against breast cancer may partly derive from synergy amongst dietary phytochemicals directed against specific molecular targets in responsive breast cancer cells, and provide support for the feasibility of the development of a diet-based combinatorial approach in the prevention and treatment of breast cancer.
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Hsieh TC,Wu JMsubject
Has Abstractpub_date
2008-10-01 00:00:00pages
851-9issue
4eissn
1019-6439issn
1791-2423journal_volume
33pub_type
杂志文章abstract::Prostate cancer is characterized by structural rearrangements, most frequently including translocations between androgen-dependent genes and members of the ETS family of transcription factor like TMPRSS2:ERG. In a recent whole genome sequencing study we identified 140 gene fusions that were unrelated to ETS genes in 1...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.2855
更新日期:2015-04-01 00:00:00
abstract::Sentinel node (SN) mapping and biopsy is a procedure that accurately stages the regional lymph node (LN) basin. Defined patterns of lymphatic drainage allow intraoperative determination of the first (sentinel) lymph node in the regional basin, and the absence of metastatic disease in the SN accurately reflects the abs...
journal_title:International journal of oncology
pub_type: 杂志文章,评审
doi:10.3892/ijo.15.1.25
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abstract::m-Chloroperoxybenzoic acid (CPBA),which induces ornithine decarboxylase activity as much as 12-O-tetradecanoylphorbol-13-acetate (TPA), was tested for its ability to induce DNA synthesis, hydroperoxide (HPx) production, and tumor promotion in mouse epidermis in vivo. After an early inhibition, CPBA stimulates DNA synt...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.8.1.197
更新日期:1996-01-01 00:00:00
abstract::The FHIT gene, at 3p14.2, has been suggested to form a molecular target to damage induced by human lung carcinogens. We examined aberrant expression of the Fhit protein and allele loss at the FHIT gene in a series of lung cancer cases, mainly of non-small cell carcinoma (NSCLC) histology. We had detailed data on tobac...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2002-02-01 00:00:00
abstract::Previous studies have revealed that protein kinase C (PKC) is responsible for malignant progression. In the present study, we investigated the potent inhibitory effects of an antiestrogen, toremifene, on PKC-mediated cellular adhesion. A phorbol ester, phorbol 12-myristate 13-acetate (PMA), significantly enhanced alph...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.13.5.981
更新日期:1998-11-01 00:00:00
abstract::The purpose of this study was to determine, using fluorescence in situ hybridization (FISH), whether treatment of cancer cells with 3'-azido-2'-3'-dideoxythymidine (AZT) has any effect on telomere length as determined by the telomeric signal intensity. To do so, we treated a metastatic murine melanoma cell line (K-173...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.13.5.923
更新日期:1998-11-01 00:00:00
abstract::MLL gene at human chromosome 11q23.3 is frequently rearranged or amplified in hematological malignancies, while PHLDB1, BCL9L, FOXN5 (FOXR1), RNF26, and MFRP genes linked to MLL gene are deleted in neuroblastoma. Here, we characterized the TMEM24 gene family by using bioinformatics. KIAA0285 gene within the 11q23.3 co...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2004-09-01 00:00:00
abstract::Conventional chemotherapy produces varying degrees of response in patients with many advanced cancers and has significant side effects. Receptors for luteinizing hormone-releasing hormone (LH-RH) are present in a high percentage of human ovarian, prostatic, breast and endometrial tumors and targeted chemotherapy based...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.12.3.671
更新日期:1998-03-01 00:00:00
abstract::Ligands of the EGF/Heregulin family control the growth of epithelial cells by binding to receptors of the erbB family. By searching a large database of cDNA sequences at Human Genome Sciences Inc. we have identified a new encoded protein sequence containing all the conserved elements of the EGF/Heregulin family. The s...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.13.5.1061
更新日期:1998-11-01 00:00:00
abstract::One of the genes that the authors have isolated by a differential display of hepatocellular carcinoma compared to adjacent liver is the alpha6 integrin. alpha6 integrin is the major adhesion receptor for laminin and is suggested to be involved in tumor cell invasion and metastasis. To our knowledge, however, there are...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.16.4.725
更新日期:2000-04-01 00:00:00
abstract::Antisense oligonucleotides have been investigated as anticancer agents administered alone or in combination with conventional chemotherapeutics. In the present study, we demonstrated synergistic effects between anti-MDM2 antisense oligonucleotides and the clinically used anticancer agent irinotecan, using nude mouse m...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2002-04-01 00:00:00
abstract::A promising family of anticancer agents, the camptothecins, is noted for their ability to induce apoptosis specifically in malignant cells. However, a major obstacle for successful cancer treatment by these and other chemotherapeutic agents is the intrinsic or acquired resistance to drug treatment. Resistance to 9NC6,...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2004-08-01 00:00:00
abstract::Developing simple and effective approaches to detect tumor markers will be critical for early diagnosis or prognostic evaluation of prostate cancer treatment. Prostate‑specific membrane antigen (PSMA) has been validated as an important tumor marker for prostate cancer progression including angiogenesis and metastasis....
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2256
更新日期:2014-03-01 00:00:00
abstract::Cytokine production in squamous cell carcinoma (SCC) of the head and neck may directly influence the clinical behavior of this aggressive neoplasm since cytokines act as autocrine/paracrine growth factors, as well as immunomodulators. In the present study, the cytokine profiles of 16 SCCs of the head and neck and matc...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
abstract::Cancer antigens RAK-p120, p42, and p25, which exhibit biological, immunological and molecular similarity to the proteins expressed by Human Immunodeficiency Virus 1 (HIV-1), were found in 47 of 47 tested cases of serous adenocarcinoma of the ovary, and 45 of 45 tested cases of squamous carcinoma of the cervix. Normal ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.9.4.693
更新日期:1996-10-01 00:00:00
abstract::The aim of this study was to examine the level and basic characteristics of cell‑derived microparticles (MPs) in the cyst fluids of odontogenic keratocysts (OKCs). For this purpose, MPs from the cyst fluids (CFMPs) of OKCs were purified by a classic differential centrifugation method and characterized by a transmissio...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2018.4361
更新日期:2018-06-01 00:00:00
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journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2017.3871
更新日期:2017-03-01 00:00:00
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journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.14.4.785
更新日期:1999-04-01 00:00:00
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journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2002-02-01 00:00:00
abstract::Percutaneous nephrostomy (PN) will rapidly correct renal failure due to ureteral obstruction. Complications of the procedure are few and the procedure can be quickly accomplished on an outpatient basis using local anesthesia. A retrospective review of fifty patients who underwent PN led to derivation of a formula whic...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.4.4.983
更新日期:1994-04-01 00:00:00
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journal_title:International journal of oncology
pub_type: 杂志文章
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更新日期:1997-11-01 00:00:00
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journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.11.3.437
更新日期:1997-09-01 00:00:00
abstract::We found that a mouse p53 cDNA clone (pP53-5; Jenkins et al, Nature 312: 651-654, 1984), which previously was characterized as encoding mutant p53 protein, in fact represents wild-type mouse p53 cDNA, as we were able to demonstrate that the mutations described represented sequencing artefacts (compressions). Such sequ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.3.1.23
更新日期:1993-07-01 00:00:00
abstract::RLIP76, a stress-responsive, multi-functional protein with multi-specific transport activity towards glutathione-conjugates (GS-E) and chemotherapeutic agents is frequently overexpressed in malignant cells. Our recent studies suggest that it plays a prominent anti-apoptotic role selectively in cancer cells. The presen...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2009-01-01 00:00:00
abstract::The distinct roles of estrogen receptors (ERs) related with androgen receptors (ARs) have been proposed in prostate cancer, while the involvement of transforming growth factor-β (TGF-β) has been reported in the progression of prostate cancer. In this study, we examined whether the TGF-β signaling pathway is associated...
journal_title:International journal of oncology
pub_type: 杂志文章
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更新日期:2014-08-01 00:00:00
abstract::Reports of the metabolomic characteristics of esophageal cancer are limited. In the present study, we thus conducted metabolome analysis of paired tumor tissues (Ts) and non-tumor esophageal tissues (NTs) using capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS). The Ts and surrounding NTs were surgi...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2018.4340
更新日期:2018-06-01 00:00:00
abstract::Tumour growth is frequently associated with massive loss of body weight and negative nitrogen balance leading to cachexia, one of the worst features of malignancy accounting for nearly two thirds of cancer deaths. At the metabolic level, cachexia is associated with depletion of body lipid stores as well as loss of ske...
journal_title:International journal of oncology
pub_type: 杂志文章,评审
doi:
更新日期:1999-03-01 00:00:00
abstract::The epithelial-to-mesenchymal transition (EMT) confers an aggressive subtype associated with chemotherapy resistance in epithelial cancers. However, the mechanisms underlying the EMT and its associated signaling dysfunctions are still poorly understood. In two genetic models of MCF-...
journal_title:International journal of oncology
pub_type: 杂志文章
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journal_title:International journal of oncology
pub_type: 杂志文章
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更新日期:2015-01-01 00:00:00
abstract::Hepatocellular carcinoma (HCC) is one of the most malignant types of cancer, and is associated with high recurrence rates and a poor response to chemotherapy. Immune signatures in the microenvironment of HCC have not been well explored systematically. The aim of the present study was to identify prognostic immune sign...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2020.4998
更新日期:2020-05-01 00:00:00