Abstract:
:The purpose of this study was to determine, using fluorescence in situ hybridization (FISH), whether treatment of cancer cells with 3'-azido-2'-3'-dideoxythymidine (AZT) has any effect on telomere length as determined by the telomeric signal intensity. To do so, we treated a metastatic murine melanoma cell line (K-1735 clone X-21) and a human breast cancer cell line (MCF-7) with three concentrations of AZT for 72 h at 37ûC. FISH preparations processed using an all-human telomeric DNA probe showed a significantly reduced, concentration-dependent telomeric signal intensity in interphase and metaphase spreads of AZT-treated cells as compared with the signal intensity in untreated controls, which showed no reduction. We conclude from these preliminary results that AZT has the potential of targeting the telomeric ends of chromosomes in cancer cells and promoting cell death and could well be tested along with other chemotherapy drugs given to cancer patients for this purpose.
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Multani AS,Furlong C,Pathak Sdoi
10.3892/ijo.13.5.923subject
Has Abstractpub_date
1998-11-01 00:00:00pages
923-5issue
5eissn
1019-6439issn
1791-2423journal_volume
13pub_type
杂志文章abstract::Identification of sensitive and specific biomarkers for early detection and prognosis of prostate cancer is essential for timely and appropriate treatment of the disease in individual patients. We identified an RNA transcript with sequence homology to TRPM8 (melastatin-related transient receptor potential member 8) th...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2010-02-01 00:00:00
abstract::The neuroepithelioma cell line CHP100 expresses low but detectable amounts of N-myc protein together with large amounts of c-myc protein. We have recently demonstrated that antisense inhibition of N-myc expression in CHP100 cells leads to decreased in vitro growth and alterations in cellular morphology without affecti...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.6.1.175
更新日期:1995-01-01 00:00:00
abstract::In order to optimize the management of patients with renal cell carcinoma (RCC) it is important to define the genetic risk for metastatic disease. In this study we performed comparative genomic hybridization (CGH) on metastatic tumors aiming at the identification of genetic alterations associated with metastatic disea...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.17.5.903
更新日期:2000-11-01 00:00:00
abstract::The modifying effects of dietary administration of capsaicin, which is the principal pungent capsicum fruit, and rotenone, which is a naturally occurring pesticide derived from Derris and Lonchorcarpus species, on azoxymethane (AOM)-induced colon tumorigenesis were investigated in male F344 rats. Gavage with capsaicin...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.19.5.929
更新日期:2001-11-01 00:00:00
abstract::A multi stage extraction procedure which gives the possibility to analyze both water soluble and lipid components stemming from the same specimen has been developed in our laboratory. Metabolites from brain cancer biopsies have been compared to metabolites in normal human brain, developing mouse brain and primary mous...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2.4.545
更新日期:1993-04-01 00:00:00
abstract::Nestin is an intermediate filament protein and a stem cell marker expressed in several tumours. There is growing evidence of an association between the expression level of nestin and the pathogenesis of triple-negative breast cancer (TNBC). Nestin is also expressed in newly forming tumour vessels and is a valuable mar...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2017.4223
更新日期:2018-02-01 00:00:00
abstract::Mos is a germ cell-specific serine/threonine protein kinase that plays an important role during meiotic divisions of oocytes. Upon expression in somatic cells, Mos causes cell cycle perturbations leading to neoplastic transformation. Mos activates the MAP kinase pathway in both oocytes and transformed somatic cells. T...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.12.5.1199
更新日期:1998-05-01 00:00:00
abstract::Simultaneous resistance of cancer cells to multiple cytotoxic drugs, multidrug resistance (MDR), is the major limitation to the successful chemotherapeutic treatment of disseminated neoplasms. The 'classical' MDR phenotype is conferred by MDR1/P-glycoprotein (MDR1/P-gp) that is expressed in almost 50% of human cancers...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2007-08-01 00:00:00
abstract::Serum CASA, CEA, CYFRA 21-1, NSE, MSA, TPA, and TPS were determined in patients with lung cancer (LC), benign lung disease (BL), and healthy control (HC) donors. Using predefined cutpoints, the cytokeratin-related markers TPA, TPS, and CYFRA showed the highest sensitivity in non-small cell lung cancer (TPA 69%, TPS 63...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.4.5.1129
更新日期:1994-05-01 00:00:00
abstract::Bcl-xL is an apoptosis inhibitor that is upregulated in bladder cancer (BCa) and provides an attractive target for molecular therapies. Treatment with specific antisense oligodeoxynucleotides (AS‑ODNs) or small interfering RNAs (siRNAs) were able to sensitize BCa cells to conventional chemotherapeutics. Ten new Bcl‑xL...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.3096
更新日期:2015-09-01 00:00:00
abstract::Cytokine production in squamous cell carcinoma (SCC) of the head and neck may directly influence the clinical behavior of this aggressive neoplasm since cytokines act as autocrine/paracrine growth factors, as well as immunomodulators. In the present study, the cytokine profiles of 16 SCCs of the head and neck and matc...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
abstract::Metformin is a commonly used oral anti-hyperglycemic agent of the biguanide family. Recent studies suggest that metformin may reduce cancer risk and improve prognosis. However, the antitumor mechanism of metformin in several types of cancers, including hepatocellular carcinoma (HCC), has not been elucidated. The goal ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2419
更新日期:2014-07-01 00:00:00
abstract::Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is promising for cancer treatment owing to its selective cytotoxicity against malignant cells. However, some cancer cell types, including malignant melanoma cells, are resistant to TRAIL-induced apoptosis. Therefore, dr...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2012.1483
更新日期:2012-08-01 00:00:00
abstract::The relationship between p53 point mutation and patient survival was examined in 328 colorectal cancer patients. Point mutation was detected in 51% (166/328) of cases and was associated with a poorer prognosis in univariate and multivariate analysis. However, subcohort analysis showed that this relationship was restri...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.15.3.491
更新日期:1999-09-01 00:00:00
abstract::Ophiopogonin B (OP-B), a saponin compound isolated from Radix Ophiopogon japonicus, was verified to inhibit cell proliferation in numerous non-small cell lung cancer (NSCLC) cells in our previous study. However, the precise mechanisms of action have remained unclear. In the present study, we mainly investigated the ef...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2016.3514
更新日期:2016-07-01 00:00:00
abstract::The mechanisms of invasion and metastasis are poorly understood. Our previous studies demonstrated that cancer cell invasion may result from reorganization of membrane molecules, thereby initiating signaling pathways. To increase our understanding on how cancer cells govern metastases we studied the established LNCaP ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo_00000302
更新日期:2009-06-01 00:00:00
abstract::Recently, the compilation of massive amounts of genetic and genomic information on a wide variety of human cancer types, collectively known as The Cancer Genome Atlas (TCGA), has revealed a wealth of descriptive classification schemes both within and between different types and sources of cancer. In endometrial cancer...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2020.5139
更新日期:2020-12-01 00:00:00
abstract::The presence of tumor cells in the bone marrow (BM) could be relevant to identifying high risk of disease progression and death in gastrointestinal cancer. However, the molecular profile associated with disseminated tumor cells (DTCs) homing to the BM has yet to be defined. MicroRNAs (miRNA) play key roles in cellular...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2011.1112
更新日期:2011-11-01 00:00:00
abstract::To elucidate the involvement of proteasome inhibition in apoptosis induced by anthracycline anticancer agents, we investigated the interaction between the proteasome and anthracycline anticancer agents, and the function of the proteasome in apoptosis. Exposure of L1210 mouse lymphocytic leukemia cells to adriamycin (A...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2002-06-01 00:00:00
abstract::Hexadecylphosphocholine (HPC, D-18506, INN: Miltefosine) was characterized in a clonogenic micro assay in vitro with respect to its cytotoxicity against a panel of human and murine tumor cell lines and murine bone marrow cultures (GM-CFC). The KB human epithelial carcinoma line was found to be more sensitive by at lea...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2.2.161
更新日期:1993-02-01 00:00:00
abstract::Gene amplification occurs frequently in human glioblastomas. We report the amplification and expression analysis of glioma amplified sequence 64 (GAS64) which was recently identified by microdissection mediated cDNA capture from the amplicon at 12q13-15. Herein we analyzed primary tumor tissues for amplification frequ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::Mutations of p53 tumor suppressor gene increase with tumor progression in colorectal cancers. In this study, we examined the expressions of p33ING1, p14ARF, MDM2 and p21WAF1 mRNA in 25 advanced colorectal cancers by quantitative RT-PCR method, and compared the expression levels of p33ING1, p14ARF, p21WAF1 and MDM2 in ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2004-10-01 00:00:00
abstract::Although a number of recent studies have reported the involvement of leptin in colorectal carcinogenesis, findings are contradictory and difficult to interpret. Our group has previously reported that leptin signaling might have an important role in the development of colorectal adenomas. In this study, we investigated...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2011.1105
更新日期:2011-10-01 00:00:00
abstract::Selenium is considered to be one of the most promising micronutrients for cancer prevention and therapy, based on evidence from epidemiological studies, laboratory-based research and clinical trial intervention. There are ample reports of selenium methionine and sodium selenite's ability to induce apoptosis in various...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2007-05-01 00:00:00
abstract::High-mobility group AT-hook protein 2 (HMGA 2) is a transcription factor associated with malignancy and poor prognosis in a variety of human cancers. We correlated HMGA 2 expression with clinical parameters, survival, and O-6-methylguanine-DNA methyltransferase methylation status (MGMT) in glioblastoma patients. HMGA ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2016.3397
更新日期:2016-04-01 00:00:00
abstract::Liver cancer is the fourth leading cause of cancer‑related mortality worldwide with limited therapeutic options. Thus, novel treatment strategies are urgently required. While the oncogenic kinase, proviral integration site for Moloney murine leukemia virus 2 (PIM2), has been shown to be overexpressed in liver cancer, ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2019.4936
更新日期:2020-02-01 00:00:00
abstract::The anti-inflammatory effects of oridonin (Ordn) have been well established in previous studies. However, the apoptotic effects of Ordn on oral cancer cells have not yet been evaluated, at least to the best of our knowledge. The aim of this study was to examine the apoptotic activity of Ordn in oral squamous cell carc...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2018.4319
更新日期:2018-05-01 00:00:00
abstract::B7-H4 is a transmembrane protein which is a member of the B7 superfamily. It is overexpressed in various types of cancer, including gastric cancer. However, the effects of B7-H4 on the tumorigenicity of gastric cancer and the underlying mechanisms have not yet been fully explored. Thus, the aim of this study was to ex...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2018.4274
更新日期:2018-04-01 00:00:00
abstract::Two new tumor-suppressor genes, the cyclin dependent kinase 4 inhibitor gene (p16/CDKN2) and the von Hippel-Lindau disease gene (VHL), have been cloned and mapped on chromosomes 9p and 3p respectively, where putative tumor-suppressor genes of the oral squamous-cell carcinoma (SCC) may be present. In order to elucidate...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.7.4.895
更新日期:1995-10-01 00:00:00
abstract::Glutathione S-transferase (GST) enzymes catalyse the detoxification of by-products of reactive oxygen species and are thus important in cellular defence mechanisms. The GSTs are polymorphic with allelic variants encoding isoforms with functional differences. GST polymorphism has been associated with susceptibility and...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00