Abstract:
:The anti-inflammatory effects of oridonin (Ordn) have been well established in previous studies. However, the apoptotic effects of Ordn on oral cancer cells have not yet been evaluated, at least to the best of our knowledge. The aim of this study was to examine the apoptotic activity of Ordn in oral squamous cell carcinoma cells and to eluciudate the underlying mechanisms. For this purpose, we employed experimental techniques, such as MTT assay, DAPI staining, soft agar assay, flow cytometry and western blot analysis. Our results revealed that Ordn suppressed oral cancer cell proliferation and soft agar colony formation, while it induced reactive oxygen species (ROS)-dependent apoptosis in a dose or time-dependent manner. The generation of ROS was detected in HN22 and HSC4 cells treated with Ordn and the use of the free radical scavenger, N-acetyl-L-cysteine, almost blocked Ordn-induced apoptosis. The phosphorylation of JNK and p38 mitogen-activated protein kinase (MAPK) was manifested in the Ordn-treated cells. Furthermore, Ordn induced the apoptosis of oral cancer cells through the mitochondrial-dependent pathway, involving the loss of mitochondrial membrane potential, the release of cytochrome c, the induction of poly(ADP-Ribose) polymerase (PARP) cleavage, alterations in the ratios of apoptotic proteins and the activation of the caspase cascade. Taken together, these findings indicate that Ordn induces the apoptosis of oral cancer cells probably via ROS-mediated JNK/p38 MAPK and mitochondrial pathways; thus, Ordn may have potential for use in the treatment of oral cancer.
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Oh HN,Seo JH,Lee MH,Yoon G,Cho SS,Liu K,Choi H,Oh KB,Cho YS,Kim H,Han AL,Chae JI,Shim JHdoi
10.3892/ijo.2018.4319subject
Has Abstractpub_date
2018-05-01 00:00:00pages
1749-1759issue
5eissn
1019-6439issn
1791-2423journal_volume
52pub_type
杂志文章abstract::In the present study, we have investigated a combination of photodynamic therapy (PDT) using 5-aminolevulinic acid (ALA) and simultaneous hyperthermia (HT) on osteosarcoma (HOSM-1) cells, squamous cell carcinoma (KB) cells and fibroblasts (HF), including an assessment of the differences in the sensitivity of these cel...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2005-07-01 00:00:00
abstract::We examined the gene expression profiles of esophageal squamous cell carcinomas (ESCCs) with respect to degree of invasive depth and lymph node (LN) involvement in a large cohort. We used high-density oligonucleotide microarrays to examine the expression of 22,115 genes in 54 ESCCs and 11 non-cancerous esophageal tiss...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2006-05-01 00:00:00
abstract::m-Chloroperoxybenzoic acid (CPBA),which induces ornithine decarboxylase activity as much as 12-O-tetradecanoylphorbol-13-acetate (TPA), was tested for its ability to induce DNA synthesis, hydroperoxide (HPx) production, and tumor promotion in mouse epidermis in vivo. After an early inhibition, CPBA stimulates DNA synt...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.8.1.197
更新日期:1996-01-01 00:00:00
abstract::Thymidine kinase (TK) activity in rat hepatoma JB1 and AH136B was mainly due to cytosolic TK and was much higher than that in rat regenerating liver. Two forms of cytosolic TK, designated as TK-I and TK-II, were revealed in addition to mitochondrial TK in anion-exchange high performance liquid chromatography (HPLC) of...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:1999-08-01 00:00:00
abstract::Loss of E-cadherin and catenin expression may be associated with distant and lymph node metastases in breast cancer. Heterogeneity of E-cadherin expression is associated with poor prognosis, suggesting that E-cadherin and catenins may serve as useful prognostic markers for invasive breast carcinoma. Reduction or loss ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2001-03-01 00:00:00
abstract::Upregulation of nuclear factor-κB (NF-κB) in colorectal carcinoma (CRC) accelerates tumor growth, whereas, irinotecan (CPT-11)-induced NF-κB activation reduces chemosensitivity and weakens the anti-colorectal cancer function itself, while proteasome inhibitors can inhibit NF-κB and improve the effect of chemotherapy. ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2513
更新日期:2014-09-01 00:00:00
abstract::In the present study, we report the utility of transrectal ultrasonography guidance for laparoscopic radical prostatectomy (LRP), and the effect of a novel surgical approach of 'seven key elements of operative skill for the early recovery of urinary continence' ('7 key elements'). Among 170 patients who underwent lapa...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2010.865
更新日期:2011-02-01 00:00:00
abstract::Previously, we found that the injection of zoledronic acid (ZOL) into mice bearing tumor induced changes of the vascular structure in the tumor. In this study, we examined whether ZOL treatment could decrease interstitial fluid pressure (IFP) via change of tumor vasculature, and enhance the antitumor efficacy of lipos...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.2991
更新日期:2015-07-01 00:00:00
abstract::Mutations in p53 are the most common genetic abnormality in cancers. Arsenic trioxide (ATO) is an effective chemotherapeutic agent for the treatment of acute promyelocytic leukemia (APL) and is being tested in phase II studies in various types of cancers. We have shown that ATO is a potent inducer of apoptosis in mult...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2007-04-01 00:00:00
abstract::Photodynamic therapy (PDT) is a tool against neoplastic and non-neoplastic diseases. PDT is capable to induce different cell death mechanisms in vitro, triggered in a dose-dependent manner. Relationships between PDT and apoptosis or necrosis induction are well-known, but other cell death mechanisms triggered after PDT...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo_32_6_1189
更新日期:2008-06-01 00:00:00
abstract::We review our recent experience with intensity-modulated radiation therapy (IMRT) and conventional three-dimensional radiation therapy (C3DRT) in advanced head and neck cancer. Sixty-nine patients with Stage IV head and neck cancer (and stage III base of tongue and hypopharynx) enrolled in a Phase II study of definiti...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2006-05-01 00:00:00
abstract::Linear accelerator-based radiotherapy has little effect on tumors such as malignant melanoma, various types of sarcoma, and most locally-advanced neoplasms that have grown to several centimeters or more. These tumors contain many hypoxic cancer cells or large amounts of anti-oxidative enzymes, and are therefore resist...
journal_title:International journal of oncology
pub_type: 临床试验,杂志文章
doi:10.3892/ijo.2011.1069
更新日期:2011-09-01 00:00:00
abstract::Meningiomas are the most commonly occurring tumors of the central nervous system including the brain and spinal cord. Malignant meningiomas are highly aggressive and frequently recur after surgical resection of the tumor. Our previous studies have reported that urokinase plasminogen activator receptor (uPAR) and matri...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2007-07-01 00:00:00
abstract::It has previously been suggested that some tumours may elaborate substances which can suppress the functional activity of lymphokine activated killer (LAK) cells. We have investigated the effects of secreted products from the colorectal cancer cell line LoVo on the pattern of expression of T lymphocyte subsets and nat...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.3.4.763
更新日期:1993-10-01 00:00:00
abstract::Side population (SP) cells are isolated from various tissues and cell lines based on the exclusion of DNA-binding dye Hoechst 33,342 and exhibit potent stem cell characteristics. There have been few previous reports of SP cells in head and neck cancer cell lines. Thus, we isolated SP cells from oral squamous cell carc...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2009-08-01 00:00:00
abstract::Globally, breast cancer in women is the leading cause of cancer death. This fact has generated an interest to obtain insight into breast tumorigenesis and also to develop drugs to control the disease. Ras is a proto-oncogene that is activated as a response to extracellular signals. As a member of the Ras GTPase superf...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.3298
更新日期:2016-02-01 00:00:00
abstract::Epithelial-mesenchymal transition (EMT) is thought to be a key step for cancer metastasis. Using an immunohistochemical approach with gastric carcinoma tissue, we found the expression of protease-activated receptor-1 (PAR1), along with a metalloproteinase known to activate PAR1, were associated with poorer prognosis, ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2651
更新日期:2014-12-01 00:00:00
abstract::The impacts of post‑operative abdominal infectious complications increase hematogenous distant metastasis and result in poor long‑term survival after curative resection. Even if curative resection can be performed, the presence of circulating tumor cells is affected. The liver, the most common site of metastases, is a...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2020.5102
更新日期:2020-10-01 00:00:00
abstract::Human telomerase reverse transcriptase (hTERT), the catalytic subunit of telomerase, is very closely associated with telomerase activity. Telomerase has been implicated in cellular immortalization and carcinogenesis. In situ detection of hTERT will aid in determining the localization of telomerase-positive cells. The ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2005-11-01 00:00:00
abstract::Lung cancer is the most common and most lethal type of cancer. A sustained proliferative capacity is one of the hallmarks of cancer, and microtubules serve an important role in maintaining a sustained cell cycle. Therefore, understanding the regulation of microtubule proteins in the cell cycle is important for tumor p...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2019.4805
更新日期:2019-07-01 00:00:00
abstract::Abnormal activation of signal transducer and activator of transcription 3 (STAT3) serves a pivotal role in oral squamous cell carcinoma (OSCC) tumor cell invasion into normal tissues or distant organs. However the downstream regulatory network of STAT3 signaling remains unclear. The present study aimed to investigate ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2018.4293
更新日期:2018-04-01 00:00:00
abstract::Polo‑like kinase 4 (PLK4) has been identified as an oncogene, which is overexpressed in various types of human cancer; however, its role in colorectal cancer (CRC) development remains unknown. The present study demonstrated that PLK4 protein expression was upregulated in CRC tissues compared with in normal tissues thr...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2018.4659
更新日期:2019-02-01 00:00:00
abstract::Fatty acid synthase (FASN) is a key enzyme that plays a critical role in numerous metabolic functions by catalyzing the synthesis for long-chain fatty acids. FASN is highly expressed in various human cancers. This preferential expression makes FASN an attractive target for anticance...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2012.1401
更新日期:2012-06-01 00:00:00
abstract::Angiogenesis is a prerequisite for the growth and metastasis of solid tumors. Studies have confirmed that in the absence of angiogenesis, tumors rarely have the ability to develop beyond a few millimeters in diameter. Tumor-associated endothelium is activated by the production of soluble factors by tumor and stromal c...
journal_title:International journal of oncology
pub_type: 杂志文章,评审
doi:
更新日期:2004-08-01 00:00:00
abstract::We studied the expresssion of estrogen (ER), progesterone (PgR), and androgen receptors (AR) in hepatocellular carcinoma (HCC) and found expression in (7/36), 3.2% (1/31), 16.7% (4/24) of cases, respectively. The expression of ER did not correlate with histological grade, tumor size, or stage of disease. On the other ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.4.6.1349
更新日期:1994-06-01 00:00:00
abstract::We recently demonstrated lymphoma development in transgenic mice deficient in retinoic acid receptor alpha (RARalpha). High incidence of lymphoma development in this transgenic mouse model system was similar to lymphoma development in p53 knockout mice. In an effort to understand the molecular basis of lymphomagenesis...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.16.3.561
更新日期:2000-03-01 00:00:00
abstract::Cytokines of the TGF beta family are thought to be involved in cellular growth control and are therefore likely candidates to regulate homeostasis of the prostate. We have analyzed immunohistochemically the expression of TGF-beta 3 in normal prostate (NP), benign prostate hyperplasia (BPH) and prostate cancer (PCa). I...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.11.6.1185
更新日期:1997-12-01 00:00:00
abstract::Bcl-xL is an apoptosis inhibitor that is upregulated in bladder cancer (BCa) and provides an attractive target for molecular therapies. Treatment with specific antisense oligodeoxynucleotides (AS‑ODNs) or small interfering RNAs (siRNAs) were able to sensitize BCa cells to conventional chemotherapeutics. Ten new Bcl‑xL...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.3096
更新日期:2015-09-01 00:00:00
abstract::Metastasis is the main challenge of osteosarcoma treatment. Herein, we first reveal the oncogenic role of FAT10 in metastasis of osteosarcoma. FAT10 was upregulated in osteosarcoma, especially in metastatic osteosarcoma. High level of FAT10 was associated with poorer prognosis of osteosarcoma patients. Moreover, Trans...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2016.3549
更新日期:2016-08-01 00:00:00
abstract::Hepatocellular carcinoma (HCC) is difficult to diagnose early, resulting in only 30% resection rate. HCC is a relatively chemo-resistant tumor, molecular targeted therapy can only benefit approximately 30% patients with liver cancer. Bufalin (Bu) is one of the topoisomerase II inhibitors, many studies have recently fo...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2016.3667
更新日期:2016-10-01 00:00:00