Abstract:
:To elucidate the involvement of proteasome inhibition in apoptosis induced by anthracycline anticancer agents, we investigated the interaction between the proteasome and anthracycline anticancer agents, and the function of the proteasome in apoptosis. Exposure of L1210 mouse lymphocytic leukemia cells to adriamycin (ADM) or 4'-O-tetrahydropyranyladriamycin (THP) resulted in apoptosis in a dose-dependent manner: 5 microM ADM and 0.5 microM THP induced apoptosis efficiently, but the effects of 10 microM ADM and 5 microM THP were markedly decreased or completely absent. Carbobenzoxy-leucyl-leucyl-leucinal (Z-LLLal), a specific proteasome inhibitor, also induced dose-dependent apoptosis of the cells. The inhibitory effect of THP on chymotrypsin-like protease activity of proteasomes purified from the cytosol of L1210 cells was stronger than that of ADM. Both of these agents showed reversible non-competitive inhibitory effects. The intracellular content of ubiquitinated protein increased in ADM-, THP- or Z-LLLal-treated L1210 cells during apoptosis. These results suggested that anthracycline anticancer agents induce apoptosis by interacting, at least in part, with proteasomes.
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Kiyomiya K,Kurebe M,Nakagawa H,Matsuo Skeywords:
subject
Has Abstractpub_date
2002-06-01 00:00:00pages
1205-9issue
6eissn
1019-6439issn
1791-2423journal_volume
20pub_type
杂志文章abstract::The Drosophila argos protein is a natural ligand antagonist of Drosophila EGF receptor (DER), but does not interact with the human EGF receptor (EGFR). With a view to generating a corresponding EGFR antagonist, we, have designed five chimeric proteins featuring selected characteristic parts of argos within the framewo...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.10.4.677
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journal_title:International journal of oncology
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journal_title:International journal of oncology
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journal_title:International journal of oncology
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journal_title:International journal of oncology
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pub_type: 杂志文章
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journal_title:International journal of oncology
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journal_title:International journal of oncology
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journal_title:International journal of oncology
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journal_title:International journal of oncology
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journal_title:International journal of oncology
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更新日期:1995-04-01 00:00:00
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journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2013.1860
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pub_type: 杂志文章
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journal_title:International journal of oncology
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journal_title:International journal of oncology
pub_type: 杂志文章
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journal_title:International journal of oncology
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