Abstract：
:Dysregulated expression of microRNAs (miRNAs) in various tissues has been associated with a variety of diseases, including cancers. Here we demonstrate that miRNAs are present in the serum and plasma of humans and other animals such as mice, rats, bovine fetuses, calves, and horses. The levels of miRNAs in serum are stable, reproducible, and consistent among individuals of the same species. Employing Solexa, we sequenced all serum miRNAs of healthy Chinese subjects and found over 100 and 91 serum miRNAs in male and female subjects, respectively. We also identified specific expression patterns of serum miRNAs for lung cancer, colorectal cancer, and diabetes, providing evidence that serum miRNAs contain fingerprints for various diseases. Two non-small cell lung cancer-specific serum miRNAs obtained by Solexa were further validated in an independent trial of 75 healthy donors and 152 cancer patients, using quantitative reverse transcription polymerase chain reaction assays. Through these analyses, we conclude that serum miRNAs can serve as potential biomarkers for the detection of various cancers and other diseases.
journal_name
Cell Resjournal_title
Cell researchauthors
Chen X,Ba Y,Ma L,Cai X,Yin Y,Wang K,Guo J,Zhang Y,Chen J,Guo X,Li Q,Li X,Wang W,Zhang Y,Wang J,Jiang X,Xiang Y,Xu C,Zheng P,Zhang J,Li R,Zhang H,Shang X,Gong T,Ning G,Wang J,Zen K,Zhang J,Zhang CYdoi
10.1038/cr.2008.282subject
Has Abstractpub_date
2008-10-01 00:00:00pages
997-1006issue
10eissn
1001-0602issn
1748-7838pii
cr2008282journal_volume
18pub_type
杂志文章相关文献
CELL RESEARCH文献大全abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Cell research
pub_type： 已发布勘误
doi：10.1038/s41422-020-0311-z
更新日期：2020-06-01 00:00:00
abstract：:Various factors/pathways including hormonal regulation have been suggested to control herpes simplex virus type 1 (HSV-1) latency and reactivation. Our computer analysis identified a DNA repeat containing thyroid hormone-responsive elements (TRE) in the regulatory region of HSV-1 latency-associated transcript (LAT). T...
journal_title：Cell research
pub_type： 杂志文章
doi：10.1038/cr.2010.50
更新日期：2010-05-01 00:00:00
abstract：:Aging is associated with many complex diseases. Reliable prediction of the aging process is important for assessing the risks of aging-associated diseases. However, despite intense research, so far there is no reliable aging marker. Here we addressed this problem by examining whether human 3D facial imaging features c...
journal_title：Cell research
pub_type： 杂志文章
doi：10.1038/cr.2015.36
更新日期：2015-05-01 00:00:00
abstract：:Transfer RNA (tRNA) is present at tens of millions of transcripts in a human cell and is the most abundant RNA in moles among all cellular RNAs. tRNA is also the most extensively modified RNA with, on an average, 13 modifications per molecule. The primary function of tRNA as the adaptor of amino acids and the genetic ...
journal_title：Cell research
pub_type： 杂志文章,评审
doi：10.1038/s41422-018-0013-y
更新日期：2018-04-01 00:00:00
abstract：:Immunization with inactivated autoreactive T cells is an effective therapeutic approach to ameliorating autoimmune diseases, while the underlying mechanisms that regulate autoreactive T cells are not completely understood. This study tested the hypothesis that T-cell vaccination (TCV) inhibits autoimmune diabetes in m...
journal_title：Cell research
pub_type： 杂志文章
doi：10.1038/cr.2011.74
更新日期：2011-09-01 00:00:00
abstract：:There is strong relationship between melanocortin-1 receptor (MC1R) gene variants and human hair color and skin type. Based on a sequencing study of MC1R gene in 50 individuals from the Uygur, Tibetan, Wa and Dai ethnic populations, we discuss the occurrence of 7 mc1r variants consisting of 5 nonsynonymous sites (Val6...
journal_title：Cell research
pub_type： 杂志文章
doi：10.1038/sj.cr.7290070
更新日期：2001-03-01 00:00:00
abstract：:LRP16 was previously identified as an estrogen-induced gene in breast cancer cells. The responsiveness of LRP16 to estrogen and its functional effects in endometrial cancer (EC) cells are still unclear. Here, we show that the mRNA level and promoter activity of the LRP16 gene were significantly increased by 17beta-est...
journal_title：Cell research
pub_type： 杂志文章
doi：10.1038/cr.2007.79
更新日期：2007-10-01 00:00:00
abstract：:The tumor suppressor PTEN controls a variety of biological processes including cell proliferation, growth, migration, and death. As a master cellular regulator, PTEN itself is also subjected to deliberated regulation to ensure its proper function. Defects in PTEN regulation have a profound impact on carcinogenesis. In...
journal_title：Cell research
pub_type： 杂志文章,评审
doi：10.1038/cr.2008.83
更新日期：2008-08-01 00:00:00
abstract：:Elimination of misfolded proteins of the endoplasmic reticulum (ER) requires their retrotranslocation from the ER to the cytosol via membrane-bound ubiquitin ligase complexes. Baldridge and Rapoport now reconstitute a key step of retrotranslocation, demonstrating a protein conduit gated by ubiquitination. ...
journal_title：Cell research
pub_type： 评论,杂志文章
doi：10.1038/cr.2016.92
更新日期：2016-10-01 00:00:00
abstract：:Autophagy is a conserved process that delivers cytosolic substances to the lysosome for degradation, but its direct role in the regulation of antiviral innate immunity remains poorly understood. Here, through high-throughput screening, we discovered that CCDC50 functions as a previously unknown autophagy receptor that...
journal_title：Cell research
pub_type： 杂志文章
doi：10.1038/s41422-020-0362-1
更新日期：2021-01-01 00:00:00
abstract：:The study of the proteins that bind to telomeric DNA in mammals has provided a deep understanding of the mechanisms involved in chromosome-end protection. However, very little is known on the binding of these proteins to nontelomeric DNA sequences. The TTAGGG DNA repeat proteins 1 and 2 (TRF1 and TRF2) bind to mammali...
journal_title：Cell research
pub_type： 杂志文章
doi：10.1038/cr.2011.40
更新日期：2011-07-01 00:00:00
abstract：:Eosinophils (Eos) have been long considered as end-stage effector cells in the hierarchical hematopoietic system. Numerous lines of evidence have suggested that Eos are multifunctional leukocytes with respect to the initiation, propagation and regulation of various inflammatory or immune reactions, especially in aller...
journal_title：Cell research
pub_type： 杂志文章
doi：10.1038/cr.2018.2
更新日期：2018-03-01 00:00:00
abstract：:A novel ribosome-inactivating protein designated Moschatin from the mature seeds of pumpkin (Cucurbita moschata) has been successively purified to homogeneity, using ammonium sulfate precipitation, CM-cellulose 52 column chromatography, Blue Sepharose CL-6B Affinity column chromatography and FPLC size-exclusion column...
journal_title：Cell research
pub_type： 杂志文章
doi：10.1038/sj.cr.7290182
更新日期：2003-10-01 00:00:00
abstract：:Our previous study has demonstrated that CD146 molecule is a biomarker on vascular endothelium, which is involved in angiogenesis and tumor growth. However the mechanism behind is not clear. Here we have for the first time developed a novel CD146 blockade system using CD146 siRNA to study its function on endothelial c...
journal_title：Cell research
pub_type： 杂志文章
doi：10.1038/sj.cr.7310039
更新日期：2006-03-01 00:00:00
abstract：:DNA hydrolysis is a biochemical process often associated with different forms of cell death, including apoptosis. In a recent paper published in Cell Discovery, Du et al. report that synaptic acetylcholinesterase (AChE-S) shows an unexpected enzymatic activity as DNase switched on after cytotoxic insults. ...
journal_title：Cell research
pub_type： 评论,杂志文章
doi：10.1038/cr.2015.52
更新日期：2015-06-01 00:00:00
abstract：:Spontaneous Ca2+ oscillations in vascular smooth muscle cells have been modeled using a single Ca2+ pool. This report describes spontaneous Ca2+ oscillations dependent on two separate Ca2+ sources for the nuclear versus cytoplasmic compartments. Changes in free intracellular Ca2+ were monitored with ratiometric Ca2+- ...
journal_title：Cell research
pub_type： 杂志文章
doi：10.1038/sj.cr.7290238
更新日期：2004-10-01 00:00:00
abstract：:CD8 engagement with class I major histocompatibility antigens greatly enhances T-cell activation, but it is not clear how this is achieved. We address the question of whether or not the antibody-mediated ligation of CD8 alone induces transcriptional remodeling in a T-cell clone, using serial analysis of gene expressio...
journal_title：Cell research
pub_type： 杂志文章
doi：10.1038/cr.2008.56
更新日期：2008-06-01 00:00:00
abstract：:Upon encountering the antigen (Ag), the immune system can either develop a specific immune response or enter a specific state of unresponsiveness, tolerance. The response of B cells to their specific Ag can be activation and proliferation, leading to the immune response, or anergy and activation-induced cell death (AI...
journal_title：Cell research
pub_type： 杂志文章,评审
doi：10.1038/sj.cr.7290047
更新日期：2000-09-01 00:00:00
abstract：:Protein ubiquitination by E3 ubiquitin ligases plays an important role in cancer development. In this study, we provide experimental evidence that a RING-finger-containing protein RNF13 is an ER/Golgi membrane-associated E3 ubiquitin ligase and its RING finger domain is required for the ubiquitin ligase activity. Immu...
journal_title：Cell research
pub_type： 杂志文章
doi：10.1038/cr.2008.285
更新日期：2009-03-01 00:00:00
abstract：:The cytotoxic T lymphocyte antigen-4 (CTLA-4)-blocking antibody ipilimumab induces immune-mediated long-term control of metastatic melanoma in a fraction of patients. Although ipilimumab undoubtedly exerts its therapeutic effects via immunostimulation, thus far clinically useful, immunologically relevant biomarkers th...
journal_title：Cell research
pub_type： 杂志文章
doi：10.1038/cr.2015.3
更新日期：2015-02-01 00:00:00
abstract：:The nucleotide sequence deduced from the amino acid sequence of the scorpion insectotoxin AaIT was chemically synthesized and was expressed in Escherichia coli. The authenticity of this in vitro expressed peptide was confirmed by N-terminal peptide sequencing. Two groups of bioassays, artificial diet incorporation ass...
journal_title：Cell research
pub_type： 杂志文章
doi：10.1038/sj.cr.7290120
更新日期：2002-06-01 00:00:00
abstract：:The Wnt/β-catenin pathway plays important roles in the differentiation of multiple cell types, including mesenchymal stem cells. Using a cell-based chemical screening assay with a synthetic chemical library of 270 000 compounds, we identified the compound SKL2001 as a novel agonist of the Wnt/β-catenin pathway and unc...
journal_title：Cell research
pub_type： 杂志文章
doi：10.1038/cr.2011.127
更新日期：2012-01-01 00:00:00
abstract：:One of the two X chromosomes in cells of female mammals is transcriptionally silenced in a process known as X chromosome inactivation (XCI). Initiation of XCI is regulated by the ubiquitin ligase Rnf12/RLIM, but the mechanisms by which Rnf12/RLIM mediates this process has been a mystery. A recent study by Gontan et al...
journal_title：Cell research
pub_type： 杂志文章
doi：10.1038/cr.2012.98
更新日期：2012-11-01 00:00:00
abstract：:CCAAT/enhancer binding protein alpha (C/EBPalpha) is a transcriptional regulatory factor that inhibits cell proliferation, and alternative translational initiation produces two polypeptides, C/EBPalphap30 and C/EBPalphap42. By expression profiling, it was revealed that C/EBPalphap30 specifically inhibited a unique set...
journal_title：Cell research
pub_type： 杂志文章
doi：10.1038/sj.cr.7310121
更新日期：2007-04-01 00:00:00
abstract：:The formation of long-term memory is critical for learning ability and social behaviors of humans and animals, yet its underlying mechanisms are largely unknown. We found that the efficacy of hippocampus-dependent memory consolidation is regulated by METTL3, an RNA N6-methyladenosine (m6A) methyltransferase, through p...
journal_title：Cell research
pub_type： 杂志文章
doi：10.1038/s41422-018-0092-9
更新日期：2018-11-01 00:00:00
abstract：:The mixed lineage kinase domain-like (MLKL) protein is a key factor in tumor necrosis factor-induced necroptosis. Recent studies on necroptosis execution revealed a commitment role of MLKL in membrane disruption. However, our knowledge of how MLKL functions on membrane remains very limited. Here we demonstrate that ML...
journal_title：Cell research
pub_type： 杂志文章
doi：10.1038/cr.2016.26
更新日期：2016-05-01 00:00:00
abstract：:Recent evidence indicates that codon optimality is a broad determinant of mRNA stability. A study by Radhakrishnan et al. in Cell raises the possibility that the conserved DEAD-box protein Dhh1 underlies the phenomenon. ...
journal_title：Cell research
pub_type： 评论,杂志文章
doi：10.1038/cr.2016.127
更新日期：2016-12-01 00:00:00
abstract：:Kinesins are microtubule-based motors involved in various intracellular transports. Neurons, flagellated cells, and pigment cells have been traditionally used as model systems to study the cellular functions of kinesins. Here, we report silkworm posterior silkgland (PSG), specialized cells with an extensive endomembra...
journal_title：Cell research
pub_type： 杂志文章
doi：10.1038/cr.2010.47
更新日期：2010-06-01 00:00:00
abstract：:We have developed a cell-free system that can trigger the nuclei purified from mouse liver and suspension-cultured carrot cells to undergo apoptosis as defined by the formation of apoptotic bodies and nucleosomal DNA fragments. The effects of different divalent cations and cycloheximide on DNA cleavage in this system ...
journal_title：Cell research
pub_type： 杂志文章
doi：10.1038/sj.cr.7290008
更新日期：1999-06-01 00:00:00
abstract：:This investigation reports a protocol for transfer and expression of foreign chimeric genes in loblolly pine (Pinus taeda L.). Transformation was achieved by co-cultivation of mature zygotic embryos with Agrobacterium tumefaciens strain LBA4404 which harbored a binary vector (pBI121) including genes for beta-glucuroni...
journal_title：Cell research
pub_type： 杂志文章
doi：10.1038/sj.cr.7290092
更新日期：2001-09-01 00:00:00
