Abstract:
:We introduced into Rhizobium leguminosarum bv. viciae LPR1105 a new pathway for the biosynthesis of the auxin, indole-3-acetic acid (IAA), under the control of a stationary phase-activated promoter active both in free-living bacteria and bacteroids. The newly introduced genes are the iaaM gene from Pseudomonas savastanoi and the tms2 gene from Agrobacterium tumefaciens. Free-living bacteria harbouring the promoter-iaaMtms2 construct release into the growth medium 14-fold more IAA than the wild-type parental strain. This IAA overproducing R. l. viciae, the RD20 strain, elicits the development of vetch root nodules containing up to 60-fold more IAA than nodules infected by the wild-type strain LPR1105. Vetch root nodules derived from RD20 are fewer in number per plant, heavier in terms of dry weight and show an enlarged and more active meristem. A significant increase in acetylene reduction activity was measured in nodules elicited in vetch by RD20.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Camerini S,Senatore B,Lonardo E,Imperlini E,Bianco C,Moschetti G,Rotino GL,Campion B,Defez Rdoi
10.1007/s00203-008-0365-7subject
Has Abstractpub_date
2008-07-01 00:00:00pages
67-77issue
1eissn
0302-8933issn
1432-072Xjournal_volume
190pub_type
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