Abstract:
:Little information is available upon the effects of carbon nanotubes (CNT) on the airway barrier. Here we study the barrier function of Calu-3 human airway epithelial cells, grown on permeable filters, after the exposure to commercial single-walled or multi-walled CNT, produced through chemical vapour deposition. To assess changes in the paracellular permeability of CNT-treated Calu-3 monolayers, we have measured the trans-epithelial electrical resistance (TEER) and the permeability to mannitol. Multi-walled CNT caused a large decrease in TEER and an increase in mannitol permeability but no substantial alteration in monolayer viability. Single-walled CNT produced much smaller changes of TEER while CNT, synthesized through the arc discharge method, and Carbon Black nanoparticles had no effect. If commercial multi-walled CNT were added during the formation of the tight monolayer, no further increase in trans-epithelial resistance was observed. Moreover, the same nanomaterials, but neither single-walled counterparts nor Carbon Black, prevented the TEER recovery observed after the discontinuation of interleukin-4, a Th2 cytokine that causes a reversible barrier dysfunction in airway epithelia. These findings suggest that commercial multi-walled CNT interfere with the formation and the maintenance of tight junctional complexes in airway epithelial cells.
journal_name
Toxicol Lettjournal_title
Toxicology lettersauthors
Rotoli BM,Bussolati O,Bianchi MG,Barilli A,Balasubramanian C,Bellucci S,Bergamaschi Edoi
10.1016/j.toxlet.2008.02.007subject
Has Abstractpub_date
2008-05-05 00:00:00pages
95-102issue
2eissn
0378-4274issn
1879-3169pii
S0378-4274(08)00059-3journal_volume
178pub_type
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