Abstract：
:Inorganic arsenic has strong human carcinogenic potential, but the availability of an animal model to study toxicity is extremely limited. Here, we used the transgenic zebrafish line Tg(k18(2.9):RFP) as an animal model to study arsenite toxicity. This line was chosen because the red fluorescent protein (RFP) is expressed in stratified epithelia (including skin), due to the RFP reporter driven by the promoter of the zebrafish keratin 18 gene. We titrated doses of inorganic arsenite for zebrafish embryos and found that arsenite exposure at 50 microM for 120 h was suitable for mimicking a long-term, chronic effect. When embryos derived from Tg(k18(2.9):RFP) adults were treated with this arsenite dose and time of exposure, abnormal phenotypes were not noticeable under the light microscope. However, arsenic keratosis was visible in the epithelial cells under the fluorescent microscope. Morphological defects became more severe with increased dose and exposure duration, suggesting that the severity of skin lesions was dose- and time-dependent. Histochemical examination of keratosis after 4',6'-diamidino-2-phenylindole hydrochloride (DAPI) staining showed that the epithelial cells overproliferated after treatment with arsenite. Therefore, this Tg(k18(2.9):RFP) zebrafish line is an excellent model for studying toxicity induced by inorganic arsenite and may have potential for studying other environmental pollutants.
journal_name
Toxicol Lettjournal_title
Toxicology lettersauthors
Wang YH,Chen YH,Wu TN,Lin YJ,Tsai HJdoi
10.1016/j.toxlet.2005.10.024keywords:
subject
Has Abstractpub_date
2006-06-01 00:00:00pages
191-7issue
3eissn
0378-4274issn
1879-3169pii
S0378-4274(05)00365-6journal_volume
163pub_type
杂志文章abstract：:It is known in humans and mouse models, that drinking water exposures to arsenite (As+3) leads to immunotoxicity. Previously, our group showed that certain types of immune cells are extremely sensitive to arsenic induced genotoxicity. In order to see if cells from different immune organs have differential sensitivitie...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2016.09.008
更新日期：2016-11-16 00:00:00
abstract：:Nano-materials are currently being used in a variety of fields. One of the concerns associated with their use is their potential to harm human health. In an attempt to identify genes expressed differently in human lung cells (WI-26 VA4) exposed to nanosized (45 nm in diameter) PAMAM (polyamidoamine) dendrimers, we obs...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2009.07.018
更新日期：2009-10-28 00:00:00
abstract：:The effects of zinc on the lipid components of carp erythrocytes (Cyprinus carpio L.) were studied on red blood cells exposed to ZnSO4 (0.01-1 mM) for 20 h. The temperatures of the incubation medium were 5 and 20 degrees C for carp collected in winter and summer, respectively. It was observed that increase in the conc...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/s0378-4274(02)00066-8
更新日期：2002-06-07 00:00:00
abstract：:Glutathione (GSH) levels, glutathione peroxidase (GPx), glutathione reductase (GR) and glutathione-S-transferase (GST) as antioxidant defense system were evaluated in CHO-K1 cells after beauvericin (BEA) exposure. The effect of N-acetyl-cysteine (NAC) pre-treatment was assessed. GSH levels significantly decrease 18% a...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2014.02.023
更新日期：2014-05-02 00:00:00
abstract：:Most studies on the adverse effects of air pollution have focused on respiratory and cardiovascular diseases, and there are relatively few studies on eye diseases following exposure of ambient particulate matter (PM). Epidemiological and clinical researches correlating the eye and PMs have recently received attention....
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2017.12.012
更新日期：2018-03-01 00:00:00
abstract：:Colistin is a polypeptide antibiotic that effectively treats infections caused by multidrug-resistant Gram-negative bacteria, but its clinical use is limited due to nephrotoxicity. The purpose of the present study was to identify biomarkers of colistin-induced nephrotoxicity and to further characterize the mechanisms ...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2016.02.018
更新日期：2016-04-25 00:00:00
abstract：:The present study evaluated the proconvulsant liability of biapenem, a novel carbapenem antibiotic, in in vitro and in vivo experiments, in comparison with the carbapenems, imipenem/cilastatin and meropenem. Imipenem/cilastatin is a carbapenem antibiotic with known proconvulsive liability in man and in animal experime...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(95)80008-2
更新日期：1995-04-01 00:00:00
abstract：:A previous study revealed that a single i.p. administration of 6 mg/kg body wt. of mercuric chloride (MC) durably inhibits the rat cerebral microvascular Na+/K(+)-ATPase activity [1]. In this study, cerebral microvessels isolated 18 h after MC treatment were compared to those obtained from control rats and subsequentl...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(94)90127-9
更新日期：1994-02-15 00:00:00
abstract：:Exposure to phthalate esters during sexual differentiation disrupts testosterone and insulin-like three hormones resulting in malformations of androgen- and insulin-like three-dependent tissues. The current study was designed to test the hypothesis that gubernacular lesions would be more prevalent in the DEHP-treated ...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2007.03.004
更新日期：2007-05-15 00:00:00
abstract：:The liver carcinogen N-nitrosodimethylamine (NDMA) has to be metabolically activated by specific cytochromes before it can react with cellular macromolecules (e.g. proteins or DNA). Although hepatocytes are believed to be responsible for this activation, the liver tumours originate mainly from non-parenchymal cells (N...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/s0378-4274(01)00400-3
更新日期：2001-09-15 00:00:00
abstract：:Polycyclic aromatic hydrocarbons (PAHs) are contaminants that are ubiquitously found in the environment, produced through combustion of organic matter or petrochemicals, and many of which are procarcinogens. The prototypic PAH, benzo[a]pyrene (B[a]P) and the highly carcinogenic dibenzo[def,p]chrysene (DBC) are metabol...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2017.01.008
更新日期：2017-03-05 00:00:00
abstract：:Since guanidinoacetic acid (GAA), a precursor of creatine, is synthesized mainly in the proximal tubule of the kidney where gentamicin (GM) nephrotoxicity often occurs, GM-induced renal cell damage was investigated using GAA synthesis in tubular suspension as an indicator. Results obtained were as follows: (1) GAA syn...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(95)03411-0
更新日期：1995-11-15 00:00:00
abstract：:The purpose of the study was to investigate the neurotoxic effect of multi-walled carbon nanotubes (MWCNTs) on the properties of glutamatergic synaptic transmission in rat's hippocampal slices using whole-cell patch clamp technique. The amplitude and frequency of excitatory postsynaptic current (EPSC) were accessed on...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2014.06.036
更新日期：2014-09-17 00:00:00
abstract：:Induction of endotoxin tolerance in rats led to reductions in bile flow and BSP excretion. BSP serum retention or storage of BSP in the liver was not affected. Although serum alanine aminotransferase (ALT) activity was slightly elevated in endotoxin-tolerant rats, the levels of serum aspartate amino-transferase (AST) ...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(82)90151-5
更新日期：1982-05-01 00:00:00
abstract：:The aim of this study was to establish an air-liquid interface (ALI) culture of precision-cut lung slices (PCLS) for direct exposure of lung cells to gaseous contaminants. Nitrogen dioxide (NO(2)) and ozone (O(3)) were selected as model gas compounds. Acute pro-inflammatory and toxic effects of NO(2) and O(3) on live ...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2010.04.004
更新日期：2010-07-01 00:00:00
abstract：:Recently, we reported that silver nanoparticles (AgNPs) induced reactive oxygen species (ROS) generation and the resultant oxidative stress contributes to the cell damage associated with AgNPs. 8-Oxoguanine (8-oxoG) is sensitive marker of ROS-induced DNA damage. 8-Oxoguanine DNA glycosylase 1 (OGG1) is an important DN...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2011.09.002
更新日期：2011-11-30 00:00:00
abstract：:The plastic chemical bisphenol A (BPA) has recently been suspected to be a base structure of endocrine disrupting chemicals, which achieve their adverse effects by interfering with human nuclear receptors. For instance, BPA, bisphenol AF, and tetrabromo- or tetrachloro-BPA (X₄-BPA) have been characterized as binders f...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2012.05.020
更新日期：2012-07-20 00:00:00
abstract：:Considering of novel biochemical modulation by some foods and beverages, we have performed screening for green tea components that have enhancing effects on doxorubicin (DOX) induced antitumor activity. Components, such as caffeine, theanine, (-)-epigallocatechin gallate (EGCG) and flavonoids have inhibitory effects o...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/s0378-4274(99)00290-8
更新日期：2000-04-03 00:00:00
abstract：:Aluminum phosphide (AlP), a widely used fumigant and rodenticide leads to high mortality if ingested. Its toxicity is due to phosphine liberated when it comes in contact with moisture. The exact mechanism of action of phosphine is not known. In this study male Wistar rats were used. The animals received a single dose ...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2012.09.020
更新日期：2012-11-23 00:00:00
abstract：:We have designed a low-cost, compact, head-only exposure system which is easy to use and allows exposure of up to 8 or 16 small rodents depending on the chamber used with the system. Animals are exposed without anesthesia or extreme restraint. Deflection tubes at the chamber inlet and outlet serve to provide turbulent...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(87)90107-x
更新日期：1987-09-01 00:00:00
abstract：:Chlordimeform (Cdf) is readily absorbed through the skin of pesticide applicators. It also enters the body when breathed into the lungs. Cdf can be found in the urine of workers who handle or are involved in applying pesticide products which contain the chemical, even when they are wearing special protective clothing ...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(86)90069-x
更新日期：1986-10-01 00:00:00
abstract：:To characterize the accumulated hazards associated with the inhalation of gases typical of combustion products, a time-integrated value known as the fractional effective dose (FED) is used. This FED is maintained by the International Organization for Standardization (ISO) and made publicly available as the Standard IS...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2016.09.012
更新日期：2016-11-16 00:00:00
abstract：:Fourteen amino acids protected at the N-terminal and at their side chains were screened for resolving the enantiomers of V-agents by NMR. While none of the shift reagents tested showed really effective separation in proton NMR, two of them (BOC-Gln(Xan)-OH, 16, and Z-Arg(Z)2-OH), 21, with 16 superior to 21) were found...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2019.12.004
更新日期：2020-03-01 00:00:00
abstract：:Work with sub-natural levels of deuterium (D) in animals has demonstrated an anti-cancer effect of low D-concentration in water. Our objective was to investigate whether deuterium-depleted water (DDW) can overturn reverse manganese (Mn)-induced reduction in life span, using the Caenorhabditis elegans (C. elegans) as a...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2012.04.014
更新日期：2012-06-20 00:00:00
abstract：:The color additive, amaranth, was given in the diet to provide dietary levels of 0 (control), 0.03, 0.09 and 0.27%, from 5 weeks of age in F0 generation mice to 9 weeks of age in F1 generation mice, and some reproductive, developmental and behavioral parameters were measured. There was no effect on the parameters of l...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(92)90290-z
更新日期：1992-05-01 00:00:00
abstract：:A group of 20 female albino rats was exposed to mosquito coil smoke, 8 h a day, 6 days per week, for 60 days. An additional group receiving air exposure served as control. The smoke-exposed animals had a lower body weight than the controls. Smoke-induced histopathological lesions, including an inflammation of the trac...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(87)90238-4
更新日期：1987-12-01 00:00:00
abstract：:Apoptosis has been described as programmed active cell death. Overexpression of the c-Myc gene has been shown to induce apoptosis in Rat1A/Myc-ER cells when combined with a growth blocking agent. In the present study, we tested whether 4-Hydroxynonenal (HNE) or N-acetyl-L-cysteine (LNAC) affect apoptotic death in seru...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/s0378-4274(96)03816-7
更新日期：1996-12-31 00:00:00
abstract：:The involvement of neurotrophins after radiation injury during brain development were studied in pregnant mice (C 57/B1) exposed on gestation day 15 to X-ray doses of 0.02-2 Gy. Nerve growth factor protein (NGF) and different cholinergic markers were investigated on postnatal day 1 (P1) and day 21 (P21); in situ hybri...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/s0378-4274(96)03827-1
更新日期：1997-01-15 00:00:00
abstract：:Cadmium is a toxic heavy metal which has been shown to be a possible risk factor of atherosclerosis in epidemiological and experimental studies. Since intimal hyperplasia in vascular tissue is an important component of atherosclerosis, we examined the effect of cadmium on the proliferation of vascular smooth muscle ce...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/s0378-4274(98)00005-8
更新日期：1998-02-01 00:00:00
abstract：:Sulfur dioxide (SO2) is one of potential risk factors for induction and/or exacerbation of asthma, but the underlying mechanisms are not well understood. In this study, we investigate the role of SO2 in asthma using a classical asthmatic model with allergic airway inflammation by treating C57BL/6 mice with ovalbumin (...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2017.12.020
更新日期：2018-03-15 00:00:00
