A GC-TOF-MS study of the stability of serum and urine metabolomes during the UK Biobank sample collection and preparation protocols.

Abstract:

BACKGROUND:The stability of mammalian serum and urine in large metabolomic investigations is essential for accurate, valid and reproducible studies. The stability of mammalian serum and urine, either processed immediately by freezing at -80 degrees C or stored at 4 degrees C for 24 h before being frozen, was compared in a pilot metabolomic study of samples from 40 separate healthy volunteers. METHODS:Metabolic profiling with GC-TOF-MS was performed for serum and urine samples collected from 40 volunteers and stored at -80 degrees C or 4 degrees C for 24 h before being frozen at -80 degrees C. Subsequent Wilcoxon rank sum test and Principal Components Analysis (PCA) methods were used to assess whether differences in the metabolomes were detected between samples stored at 4 degrees C for 0 or 24 h. RESULTS:More than 700 unique metabolite peaks were detected, with over 200 metabolite peaks detected in any one sample. PCA and Wilcoxon rank sum tests of serum and urine data showed as a general observation that the variance associated with the replicate analysis per sample (analytical variance) was of the same magnitude as the variance observed between samples stored at 4 degrees C for 0 or 24 h. From a functional point of view the metabolomic composition of the majority of samples did not change in a statistically significant manner when stored under two different conditions. CONCLUSIONS:Based on this small pilot study, the UK Biobank sampling, transport and fractionation protocols are considered suitable to provide samples, which can produce scientifically robust and valid data in metabolomic studies.

journal_name

Int J Epidemiol

authors

Dunn WB,Broadhurst D,Ellis DI,Brown M,Halsall A,O'Hagan S,Spasic I,Tseng A,Kell DB

doi

10.1093/ije/dym281

subject

Has Abstract

pub_date

2008-04-01 00:00:00

pages

i23-30

eissn

0300-5771

issn

1464-3685

pii

dym281

journal_volume

37 Suppl 1

pub_type

杂志文章
  • Obesity prediction by modelling BMI distributions: application to national survey data from Mexico, Colombia and Peru, 1988-2014.

    abstract:BACKGROUND:The prediction of future obesity patterns is crucial for effective strategic planning. However, disproportionally changing body mass index (BMI) distributions pose particular challenges. Flexible modelling of the shape of BMI distributions may improve prediction performance. METHODS:We used data from repeat...

    journal_title:International journal of epidemiology

    pub_type: 杂志文章

    doi:10.1093/ije/dyz195

    authors: Yamada G,Castillo-Salgado C,Jones-Smith JC,Moulton LH

    更新日期:2020-06-01 00:00:00

  • It is just a game: lack of association between watching football matches and the risk of acute cardiovascular events.

    abstract:BACKGROUND:The role of trigger factors in acute cardiovascular events has been much studied in the past few years. A recent study analysed changes in the rates of cardiac emergencies in Bavaria (Germany) during the last Football World Cup. The authors reported a 2.7-fold increase in the incidence of cardiac emergencies...

    journal_title:International journal of epidemiology

    pub_type: 杂志文章

    doi:10.1093/ije/dyq007

    authors: Barone-Adesi F,Vizzini L,Merletti F,Richiardi L

    更新日期:2010-08-01 00:00:00

  • The intergenerational impact of the African orphans crisis: a cohort study from an HIV/AIDS affected area.

    abstract:BACKGROUND:In sub-Saharan Africa, the prevalence of orphanhood among children has been greatly exacerbated by the HIV/AIDS pandemic. If orphanhood harms a child's development and these effects perpetuate into adult life, then the African orphan crisis could seriously jeopardize the continent's future generations. Wheth...

    journal_title:International journal of epidemiology

    pub_type: 杂志文章

    doi:10.1093/ije/dyn197

    authors: Beegle K,De Weerdt J,Dercon S

    更新日期:2009-04-01 00:00:00

  • Is shift work associated with a higher risk of overweight or obesity? A systematic review of observational studies with meta-analysis.

    abstract:Background:An increasing number of original studies suggest that exposure to shift work could be associated with the risk of overweight and obesity, but the results remain conflicted and inconclusive. This study aimed to quantitatively synthesize available epidemiological evidence on the association between shift work ...

    journal_title:International journal of epidemiology

    pub_type: 杂志文章,meta分析

    doi:10.1093/ije/dyy079

    authors: Liu Q,Shi J,Duan P,Liu B,Li T,Wang C,Li H,Yang T,Gan Y,Wang X,Cao S,Lu Z

    更新日期:2018-12-01 00:00:00

  • Morbidity profile and its relationship with disability and psychological distress among elderly people in Northern India.

    abstract:BACKGROUND:Morbidity among elderly people has an important influence on their physical functioning and psychological well-being. Evaluation of the morbidity profile and its determinants, which have implications for elderly people, are not available. The objective of this study is to assess morbidity, co-morbidity, and ...

    journal_title:International journal of epidemiology

    pub_type: 杂志文章

    doi:10.1093/ije/dyg204

    authors: Joshi K,Kumar R,Avasthi A

    更新日期:2003-12-01 00:00:00

  • Spontaneous foetal losses in women using different contraceptives around the time of conception.

    abstract::Spontaneous losses between the 5th and 27th weeks of pregnancy were measured in a prospective study of 32 123 women whose contraceptive history around the time of conception was known. Diaphragm use prior to conception was associated with a significant reduction in second-trimester losses, after taking into account th...

    journal_title:International journal of epidemiology

    pub_type: 杂志文章

    doi:10.1093/ije/9.1.49

    authors: Harlap S,Shiono PH,Ramcharan S

    更新日期:1980-03-01 00:00:00

  • Case-control studies of the effect of environmental sanitation on diarrhoea morbidity: methodological implications of field studies in Africa and Asia.

    abstract::The problems and prospects in the use of case-control studies to assess the effects of improvements in environmental sanitation on diarrhoea morbidity are discussed on the basis of two field studies. It is concluded that an adequate design is available for assessing the effects of a single improvement on diarrhoeal di...

    journal_title:International journal of epidemiology

    pub_type: 杂志文章

    doi:10.1093/ije/17.2.441

    authors: Briscoe J,Baltazar J,Young B

    更新日期:1988-06-01 00:00:00

  • Long-term antioxidant supplementation has no effect on health-related quality of life: the randomized, double-blind, placebo-controlled, primary prevention SU.VI.MAX trial.

    abstract:BACKGROUND:The effect of antioxidant vitamin and mineral supplementation on health is one of the most controversial issues in human nutrition. Our objective was to investigate the effect of nutritional doses of a combination of antioxidant vitamins and minerals on health-related quality of life (HRQoL) in a sample of h...

    journal_title:International journal of epidemiology

    pub_type: 杂志文章,随机对照试验

    doi:10.1093/ije/dyr161

    authors: Briançon S,Boini S,Bertrais S,Guillemin F,Galan P,Hercberg S

    更新日期:2011-12-01 00:00:00

  • Thyroid disorders in the general population of Hisayama Japan, with special reference to prevalence and sex differences.

    abstract::Serum triiodothyronine (T3), thyroxine (T4), thyroid stimulating hormone (TSH) and thyroid autoantibodies (TAA) to either microsome or thyroglobulin were measured in 1251 samples from the general population, 546 males and 705 females, aged 40 or over in Hisayama, a Japanese rural town. TAA was positive in 7.7% of men ...

    journal_title:International journal of epidemiology

    pub_type: 杂志文章

    doi:10.1093/ije/16.4.545

    authors: Okamura K,Nakashima T,Ueda K,Inoue K,Omae T,Fujishima M

    更新日期:1987-12-01 00:00:00

  • Investigating the causal effect of maternal vitamin B12 and folate levels on offspring birthweight.

    abstract:BACKGROUND:Lower maternal serum vitamin B12 (B12) and folate levels have been associated with lower offspring birthweight, in observational studies. The aim of this study was to investigate whether this relationship is causal. METHODS:We performed two-sample Mendelian randomization (MR) using summary data on associati...

    journal_title:International journal of epidemiology

    pub_type: 杂志文章

    doi:10.1093/ije/dyaa256

    authors: Moen GH,Beaumont RN,Grarup N,Sommer C,Shields BM,Lawlor DA,Freathy RM,Evans DM,Warrington NM

    更新日期:2020-12-21 00:00:00

  • Software Application Profile: RVPedigree: a suite of family-based rare variant association tests for normally and non-normally distributed quantitative traits.

    abstract:MOTIVATION:RVPedigree (Rare Variant association tests in Pedigrees) implements a suite of programs facilitating genome-wide analysis of association between a quantitative trait and autosomal region-based genetic variation. The main features here are the ability to appropriately test for association of rare variants wit...

    journal_title:International journal of epidemiology

    pub_type: 杂志文章

    doi:10.1093/ije/dyw047

    authors: Oualkacha K,Lakhal-Chaieb L,Greenwood CM

    更新日期:2016-04-01 00:00:00

  • Global trend of aetiology-based primary liver cancer incidence from 1990 to 2030: a modelling study.

    abstract:BACKGROUND:Predictions of primary liver cancer (PLC) incidence rates and case numbers are critical to understand and plan for PLC disease burden. METHODS:Data on PLC incidence rates and case numbers from 1990 to 2017 were retrieved from the Global Burden of Disease database. The estimated average percentage change (EA...

    journal_title:International journal of epidemiology

    pub_type: 杂志文章

    doi:10.1093/ije/dyaa196

    authors: Liu Z,Xu K,Jiang Y,Cai N,Fan J,Mao X,Suo C,Jin L,Zhang T,Chen X

    更新日期:2020-12-08 00:00:00

  • Material, psychosocial, behavioural and biomedical factors in the explanation of relative socio-economic inequalities in mortality: evidence from the HUNT study.

    abstract:BACKGROUND:Previous studies have assessed the relative importance of material, psychosocial and behavioural factors in the explanation of relative socio-economic inequalities in mortality, but research into the contribution of biomedical factors has been limited. Our study examines the relative contribution of (i) mate...

    journal_title:International journal of epidemiology

    pub_type: 杂志文章

    doi:10.1093/ije/dyp262

    authors: Skalická V,van Lenthe F,Bambra C,Krokstad S,Mackenbach J

    更新日期:2009-10-01 00:00:00

  • Association of HIV and ART with cardiometabolic traits in sub-Saharan Africa: a systematic review and meta-analysis.

    abstract:BACKGROUND:Sub-Saharan Africa (SSA) has the highest burden of HIV in the world and a rising prevalence of cardiometabolic disease; however, the interrelationship between HIV, antiretroviral therapy (ART) and cardiometabolic traits is not well described in SSA populations. METHODS:We conducted a systematic review and m...

    journal_title:International journal of epidemiology

    pub_type: 杂志文章,meta分析,评审

    doi:10.1093/ije/dyt198

    authors: Dillon DG,Gurdasani D,Riha J,Ekoru K,Asiki G,Mayanja BN,Levitt NS,Crowther NJ,Nyirenda M,Njelekela M,Ramaiya K,Nyan O,Adewole OO,Anastos K,Azzoni L,Boom WH,Compostella C,Dave JA,Dawood H,Erikstrup C,Fourie CM,Fr

    更新日期:2013-12-01 00:00:00

  • Transmission of hepatitis B virus infection in Ethiopia with emphasis on the importance of vertical transmission.

    abstract::This two-year controlled study was conducted to define the mode of transmission of hepatitis B infection in Ethiopia. Twenty-five of 500 (5%) expectant mothers were identified as HbsAg positive (Group A). A control group of 29 mothers negative for HBsAg (Group B) was randomly selected. These 54 mothers and their famil...

    journal_title:International journal of epidemiology

    pub_type: 杂志文章

    doi:10.1093/ije/17.4.874

    authors: Tsega E,Tsega M,Mengesha B,Nordenfelt E,Hansson BG,Lindberg J

    更新日期:1988-12-01 00:00:00

  • Chlamydia pneumoniae antibodies in chronic obstructive pulmonary disease.

    abstract:BACKGROUND:The significance of persistent or recurrent respiratory infections in adult life for the development of chronic obstructive pulmonary disease (COPD) is still to a large extent unknown. A few clinical and experimental animal studies suggest that peripheral airways diseases may be due to the cumulative effects...

    journal_title:International journal of epidemiology

    pub_type: 杂志文章

    doi:10.1093/ije/25.3.658

    authors: von Hertzen L,Isoaho R,Leinonen M,Koskinen R,Laippala P,Töyrylä M,Kivelä SL,Saikku P

    更新日期:1996-06-01 00:00:00

  • Smoking among junior high school students in Nagoya, Japan.

    abstract::A questionnaire survey was administered in January 1982 to 3090 junior high school boys and girls in Nagoya, Japan. The proportion of current regular smokers who smoke at least one cigarette per week was 3.6% for boys and 0.5% for girls. By the third grade this had increased to 6.9% and 1.2% respectively for each sex....

    journal_title:International journal of epidemiology

    pub_type: 杂志文章

    doi:10.1093/ije/17.4.814

    authors: Ogawa H,Tominaga S,Gellert G,Aoki K

    更新日期:1988-12-01 00:00:00

  • Genital herpes infection: a review.

    abstract::Genital herpes infection is life-long and may result in painful and recurrent genital lesions, systemic complications, serious psychosocial morbidity, and rare but serious outcomes in neonates born to infected women, including permanent neurological handicap and death. Herpes simplex virus (HSV)-2 is the principal cau...

    journal_title:International journal of epidemiology

    pub_type: 杂志文章,评审

    doi:10.1093/ije/26.4.698

    authors: Brugha R,Keersmaekers K,Renton A,Meheus A

    更新日期:1997-08-01 00:00:00

  • Increasing mortality during the expansions of the US economy, 1900-1996.

    abstract:BACKGROUND:In Western countries mortality dropped throughout the 20th century, but over and above the long-term falling trend, the death rate has oscillated over time. It has been postulated that these short-term oscillations may be related to changes in the economy. METHODS:To ascertain if these short-term oscillatio...

    journal_title:International journal of epidemiology

    pub_type: 杂志文章

    doi:10.1093/ije/dyi141

    authors: Tapia Granados JA

    更新日期:2005-12-01 00:00:00

  • The Ballabgarh Health and Demographic Surveillance System (CRHSP-AIIMS).

    abstract::The Ballabgarh Health and Demographic Surveillance System (HDSS), also known as the Comprehensive Rural Health Services Project (CRHSP) Ballabgarh, is located in north India and was established in 1961 to develop a model for rural health-care practice in India. In addition to demographic surveillance and community-bas...

    journal_title:International journal of epidemiology

    pub_type: 杂志文章

    doi:10.1093/ije/dyt055

    authors: Kant S,Misra P,Gupta S,Goswami K,Krishnan A,Nongkynrih B,Rai SK,Srivastava R,Pandav CS

    更新日期:2013-06-01 00:00:00

  • Short stature and cardiovascular disease among men and women from two southeastern New England communities.

    abstract:BACKGROUND:Short stature has been associated with an increased risk of coronary heart disease (CHD), although the reason for the association remains unclear. Data on the relation between stature and stroke is more limited. We examined the association between stature and CHD as well as between stature and stroke in men ...

    journal_title:International journal of epidemiology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1093/ije/27.6.970

    authors: Parker DR,Lapane KL,Lasater TM,Carleton RA

    更新日期:1998-12-01 00:00:00

  • Getting more from heterogeneous HIV-1 surveillance data in a high immigration country: estimation of incidence and undiagnosed population size using multiple biomarkers.

    abstract:BACKGROUND:Most HIV infections originate from individuals who are undiagnosed and unaware of their infection. Estimation of this quantity from surveillance data is hard because there is incomplete knowledge about (i) the time between infection and diagnosis (TI) for the general population, and (ii) the time between imm...

    journal_title:International journal of epidemiology

    pub_type: 杂志文章

    doi:10.1093/ije/dyz100

    authors: Giardina F,Romero-Severson EO,Axelsson M,Svedhem V,Leitner T,Britton T,Albert J

    更新日期:2019-12-01 00:00:00

  • Exclusion of clinically atypical or microbiologically mixed diarrhoeal episodes from outcome events in a field trial of oral cholera vaccines.

    abstract::We investigated whether alternative clinical and microbiological criteria for outcome events affected estimates of vaccine efficacy in a randomized, double-blind field trial of B subunit-killed whole cell (BS-WC) and killed whole cell-only (WC) oral cholera vaccines among 62,285 rural Bangladeshi participants. At one ...

    journal_title:International journal of epidemiology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1093/ije/18.2.440

    authors: Clemens JD,Stanton BF,Harris JR,Chakraborty J,Sack DA,Rao MR,Ahmed F,Ansaruzzaman M,Yunus MD,Svennerholm AM

    更新日期:1989-06-01 00:00:00

  • Corporate influences on epidemiology.

    abstract::Corporate influences on epidemiology have become stronger and more pervasive in the last few decades, particularly in the contentious fields of pharmacoepidemiology and occupational epidemiology. For every independent epidemiologist studying the side effects of medicines and the hazardous effects of industrial chemica...

    journal_title:International journal of epidemiology

    pub_type: 杂志文章,评审

    doi:10.1093/ije/dym270

    authors: Pearce N

    更新日期:2008-02-01 00:00:00

  • Estimating interaction on an additive scale between continuous determinants in a logistic regression model.

    abstract:BACKGROUND:To determine the presence of interaction in epidemiologic research, typically a product term is added to the regression model. In linear regression, the regression coefficient of the product term reflects interaction as departure from additivity. However, in logistic regression it refers to interaction as de...

    journal_title:International journal of epidemiology

    pub_type: 杂志文章

    doi:10.1093/ije/dym157

    authors: Knol MJ,van der Tweel I,Grobbee DE,Numans ME,Geerlings MI

    更新日期:2007-10-01 00:00:00

  • Occupational exposure to respirable crystalline silica and risk of autoimmune rheumatic diseases: a nationwide cohort study.

    abstract:BACKGROUND:Exposure to respirable crystalline silica is suggested to increase the risk of autoimmune rheumatic diseases. We examined the association between respirable crystalline silica exposure and systemic sclerosis, rheumatoid arthritis, systemic lupus erythematosus and small vessel vasculitis. METHODS:In a cohort...

    journal_title:International journal of epidemiology

    pub_type: 杂志文章

    doi:10.1093/ije/dyaa287

    authors: Boudigaard SH,Schlünssen V,Vestergaard JM,Søndergaard K,Torén K,Peters S,Kromhout H,Kolstad HA

    更新日期:2021-01-18 00:00:00

  • Food insecurity, food choices, and body mass index in adults: nutrition transition in Trinidad and Tobago.

    abstract:BACKGROUND:This study evaluated whether food insecurity and obesity were associated in a population sample in Trinidad. METHODS:A sample was drawn of 15 clusters of households, in north central Trinidad. Resident adults were enumerated. A questionnaire was administered including the short form Household Food Security ...

    journal_title:International journal of epidemiology

    pub_type: 杂志文章

    doi:10.1093/ije/dyg100

    authors: Gulliford MC,Mahabir D,Rocke B

    更新日期:2003-08-01 00:00:00

  • Racial/ethnic differences in the epidemiology of ovarian cancer: a pooled analysis of 12 case-control studies.

    abstract:Background:Ovarian cancer incidence differs substantially by race/ethnicity, but the reasons for this are not well understood. Data were pooled from the African American Cancer Epidemiology Study (AACES) and 11 case-control studies in the Ovarian Cancer Association Consortium (OCAC) to examine racial/ethnic differences...

    journal_title:International journal of epidemiology

    pub_type: 杂志文章

    doi:10.1093/ije/dyx252

    authors: Peres LC,Risch H,Terry KL,Webb PM,Goodman MT,Wu AH,Alberg AJ,Bandera EV,Barnholtz-Sloan J,Bondy ML,Cote ML,Funkhouser E,Moorman PG,Peters ES,Schwartz AG,Terry PD,Manichaikul A,Abbott SE,Camacho F,Jordan SJ,Nagle C

    更新日期:2018-04-01 00:00:00

  • Comparative prevalence of rabies antibodies among household and unclaimed/stray dogs as determined by the immune adherence haemagglutination assay.

    abstract::The immune adherence haemagglutination assay (IAHA), widely used for human viral disease diagnosis, has been adapted for detection of rabies virus antibodies in dog sera. Rabies virus antibody titres obtained by the IAHA correlated well with those obtained by the currently accepted test for rabies antibody determinati...

    journal_title:International journal of epidemiology

    pub_type: 杂志文章

    doi:10.1093/ije/16.3.472

    authors: Bota CN,Anderson RK,Goyal SM,Charamella LJ,Howard DR,Briggs DJ

    更新日期:1987-09-01 00:00:00

  • Low-dose aspirin use and endometrial cancer mortality-a Danish nationwide cohort study.

    abstract:BACKGROUND:Accumulating evidence suggests that aspirin use may improve survival in cancer patients, however, for endometrial cancer, epidemiological evidence is limited and results are equivocal. In a nationwide cohort study, we examined the association between post-diagnostic low-dose aspirin use and endometrial cance...

    journal_title:International journal of epidemiology

    pub_type: 杂志文章

    doi:10.1093/ije/dyz253

    authors: Sperling CD,Verdoodt F,Aalborg GL,Dehlendorff C,Friis S,Kjaer SK

    更新日期:2020-02-01 00:00:00