Abstract:
:The purpose of this study was to evaluate the effect of osteogenic differentiation of bone marrow stromal stem cells (BMSCs) on bone formation in a novel interconnected porous calcium hydroxyapatite (IP-CHA). BMSCs/IP-CHA composites, as a cell-hybrid artificial bone, were made by injecting BMSCs solution into IP-CHA scaffolds. To induce osteogenic differentiation, BMSCs/IP-CHA composites were subcultured for three, seven, 10, and 14 days. At the end of each subculture period, BMSCs/IP-CHA composites were examined by SEM and ALP staining. BMSCs/IP-CHA composites of different osteogenic groups of subculture were also placed into bone sockets in the right femur of beagle dogs. After four weeks, same placement procedure was done in the left femur. BMSCs/IP-CHA subcultured for 10 and 14 days were ALP-positive as opposed to those of three and seven days. At four weeks after placement, bone formation was superior at the 10- and 14-day subculture groups. Based on the results obtained, it was suggested that osteogenic differentiation periods with 10 and 14 days of subculture for BMSCs/IP-CHA as a cell-hybrid artificial bone were beneficial in promoting bone formation.
journal_name
Dent Mater Jjournal_title
Dental materials journalauthors
Doi K,Kubo T,Hayashi K,Imura K,Akagawa Ydoi
10.4012/dmj.26.162subject
Has Abstractpub_date
2007-03-01 00:00:00pages
162-9issue
2eissn
0287-4547issn
1881-1361journal_volume
26pub_type
杂志文章abstract::The aim of this study was to clarify the effect of heating rate on the development of both transient and residual stresses in investment molds. Solid, cylindrical, gypsum-based molds were modeled and theoretical calculations made of temperature distributions with a constant heating rate. These calculations used experi...
journal_title:Dental materials journal
pub_type: 杂志文章
doi:10.4012/dmj.15.121
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abstract::The influence of the number of cycles on shear fatigue strength to enamel and dentin using dental adhesives in self-etch mode was investigated. A two-step self-etch adhesive and two universal adhesives were used to bond to enamel and dentin in self-etch mode. Initial shear bond strength and shear fatigue strength to e...
journal_title:Dental materials journal
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doi:10.4012/dmj.26.628
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journal_title:Dental materials journal
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abstract::The effects of monomer structure on the mechanical properties of visible light-cured composite resins based on the seven types of aromatic dimethacrylates were investigated. The results of this study suggested that the mechanical properties of the composite resins were dependent upon the chemical structure of the dime...
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journal_title:Dental materials journal
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journal_title:Dental materials journal
pub_type: 杂志文章
doi:10.4012/dmj.2015-378
更新日期:2016-01-01 00:00:00
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journal_title:Dental materials journal
pub_type: 杂志文章
doi:10.4012/dmj.2017-279
更新日期:2019-02-08 00:00:00
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journal_title:Dental materials journal
pub_type: 杂志文章
doi:10.4012/dmj.2013-060
更新日期:2013-01-01 00:00:00
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journal_title:Dental materials journal
pub_type: 杂志文章
doi:10.4012/dmj.26.481
更新日期:2007-07-01 00:00:00
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journal_title:Dental materials journal
pub_type: 杂志文章
doi:10.4012/dmj.14.221
更新日期:1995-12-01 00:00:00
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journal_title:Dental materials journal
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更新日期:2003-12-01 00:00:00
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journal_title:Dental materials journal
pub_type: 杂志文章
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更新日期:2016-01-01 00:00:00
abstract::We evaluated the effect of different curing strategies for universal adhesives on micro-tensile bond strength (μTBS) between resin cement and dentin and/or between resin cement and indirect resin composite. Flat coronal dentin surfaces and composite resin disks were pretreated with silane-containing universal adhesive...
journal_title:Dental materials journal
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doi:10.4012/dmj.2017-240
更新日期:2018-06-08 00:00:00
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journal_title:Dental materials journal
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doi:10.4012/dmj.2015-099
更新日期:2015-01-01 00:00:00
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journal_title:Dental materials journal
pub_type: 杂志文章
doi:10.4012/dmj.18.304
更新日期:1999-09-01 00:00:00
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journal_title:Dental materials journal
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更新日期:2011-01-01 00:00:00
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journal_title:Dental materials journal
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更新日期:2016-01-01 00:00:00
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journal_title:Dental materials journal
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journal_title:Dental materials journal
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journal_title:Dental materials journal
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