Abstract:
:The creation of 3D scaffolds that mimic the structure of physiological tissue required for normal cell function is a major bioengineering challenge. For corneal stroma reconstruction this necessitates the creation of a stroma-like scaffold consisting of a stack of orthogonally disposed sheets of aligned collagen fibrils. This study demonstrates that such a scaffold can be built up using magnetic alignment. By allowing neutralized acid-soluble type I collagen to gel in a horizontal magnetic field (7 T) and by combining a series of gelation-rotation-gelation cycles, a scaffold of orthogonal lamellae composed of aligned collagen fibrils has been formed. Although initially dilute, the gels can be concentrated without noticeable loss in orientation. The gels are translucent but their transparency can be greatly improved by the addition of proteoglycans to the gel-forming solution. Keratocytes align by contact guidance along the direction of collagen fibrils and respect the orthogonal design of the collagen template as they penetrate into the bulk of the 3D matrix. The scaffold is a significant step towards the creation of a corneal substitute with properties resembling those of native corneal stroma.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Torbet J,Malbouyres M,Builles N,Justin V,Roulet M,Damour O,Oldberg A,Ruggiero F,Hulmes DJdoi
10.1016/j.biomaterials.2007.05.024subject
Has Abstractpub_date
2007-10-01 00:00:00pages
4268-76issue
29eissn
0142-9612issn
1878-5905pii
S0142-9612(07)00440-1journal_volume
28pub_type
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