Adsorption of plasma proteins on polyethylene oxide-modified lipid bilayers studied by total internal reflection fluorescence.

Abstract:

:Distearoylphophatidylcholine (DSPC) mixed with various mole percentages of polyethylene oxide (number average molecular weight 2000)-grafted distearoylphosphatidylethanolamine (PEO2000-DSPE) were deposited on DSPE-coated quartz surfaces by the Langmuir-Blodgett deposition. Structural transitions in PEO2000 from pancake to mushroom, and from mushroom to brush conformations were revealed from film balance experiments. Adsorption kinetics of proteins from 1% platelet-poor plasma (PPP) on the supported lipid bilayers were studied using intrinsic total internal reflection fluorescence. All the supported lipid bilayers exhibited over a magnitude reduction in adsorbed plasma proteins, compared with the quartz substrate. The increase of PEO2000-DSPE density in the mixed bilayers slightly increases the amount of adsorbed proteins on the bilayers.

journal_name

Biomaterials

journal_title

Biomaterials

authors

Xu Z,Marchant RE

doi

10.1016/s0142-9612(00)00004-1

keywords:

subject

Has Abstract

pub_date

2000-05-01 00:00:00

pages

1075-83

issue

10

eissn

0142-9612

issn

1878-5905

pii

S0142-9612(00)00004-1

journal_volume

21

pub_type

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