Abstract:
BACKGROUND:Advanced glycation end products (AGEs) play a critical role in the development of diabetic nephropathy. Reactive oxygen species (ROS) may play a critical role in AGEs induced growth factor expression. In this study, the effects of AGEs on transforming growth factor beta1 (TGF-beta1), connective tissue growth factor (CTGF) and fibronectin (Fn) mRNA expression and oxidative stress in cultured NRK-49F cells were examined. METHODS:NRK-49F cells were incubated with medium containing different doses of AGEs (50, 100 or 200 microg/ml) for 24 hours, or with AGEs 100 microg/ml for different times (0, 12, 24 or 48 hours). Cells in the serum-free medium or medium containing 25 mmol/L glucose were controls. Cells were treated with 25 mmol/L glucose and 100 microg/ml AGEs for 24 hours to determine the effects between AGEs and glucose. We clarified the role of antioxidant by pretreating cells with N-acetylcysteine (10 mmol/L), ginkgo biloba extract (50 or 100 mg/L) for 24 hours and with 100 microg/ml AGEs for further 24 hours. Alamarblue dye assay was used to analyze cell growth; intracellular ROS generation was measured by flow cytometry; intracellular glutathione by fluorescence spectrophotometry; expressions of TGF-beta1, CTGF and Fn mRNA by semiquantitative RT-PCR. RESULTS:AGEs significantly increased the expressions of TGF-beta1, CTGF, Fn mRNA and intracellular ROS generation, and decreased the glutathion level in NRK-49F cells in dose- and time-dependent manners. High glucose and AGEs together significantly increased the expression of TGF-beta1, CTGF and Fn mRNA, compared with AGEs and high glucose separately. Preincubation with N-acetylcysteine or ginkgo biloba extract increased GSH level, suppressed AGEs-induced oxidative stress and TGF-beta1, CTGF and Fn mRNA overexpression. CONCLUSIONS:AGEs can significantly increase expression of TGF-beta1, CTGF, Fn mRNA in NRK-49F cells through enhancement of oxidative stress. The accumulation of AGEs may play a pivotal role in the pathogenesis of tubulointerstitial fibrosis in diabetic nephropathy. Suppression of AGEs induced TGF-beta1, CTGF and Fn mRNA overexpression in renal fibroblasts through inhibition of oxidative stress may be a mechanism underlying effect of ginkgo biloba extract in diabetic nephropathy. In addition, antioxidant therapy may help prevent AGEs accumulation and its induced damage.
journal_name
Chin Med J (Engl)journal_title
Chinese medical journalauthors
Yan HD,Li XZ,Xie JM,Li Msubject
Has Abstractpub_date
2007-05-05 00:00:00pages
787-93issue
9eissn
0366-6999issn
2542-5641journal_volume
120pub_type
杂志文章abstract:OBJECTIVE:Intermediate-risk acute myeloid leukemia (IR-AML), which accounts for a substantial number of AML cases, is highly heterogeneous. We systematically summarize the latest research progress on the significance of gene mutations for prognostic stratification of IR-AML. DATA SOURCES:We conducted a systemic search...
journal_title:Chinese medical journal
pub_type: 杂志文章,评审
doi:10.4103/0366-6999.163400
更新日期:2015-09-05 00:00:00
abstract:OBJECTIVE:To study 4 cases of adult T-cell leukemia/lymphoma (ATLL) associated with cutaneous lesions for clinicopathology, immunophenotype, human T-cell leukemia/lymphoma virus type I (HTLV-I) provirus DNA and their ultrastructure. At the same time, HTLV-I provirus DNA of ATLL patients were also compared with 18 cases...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1999-05-01 00:00:00
abstract::Twin pregnancy with mosaic partial hydatidiform mole (PHM) and survival of two healthy fetuses following in vitro fertilization and embryos transfer (IVF-ET) is a rare situation and is considered a challenge for management. A 32-year-old Chinese woman conceived twin pregnancy following IVF-ET. At 22 weeks' gestation, ...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2012-12-01 00:00:00
abstract:BACKGROUND:The effectiveness of neoadjuvant chemoradiotherapy (NCRT) treatment for patients with esophageal carcinoma (EC) remains controversial. The aim of this study was to compare the effect of NCRT followed by surgery (NCRTS) with surgery alone (SA) for EC. METHODS:The PubMed, EMBASE, and the Cochrane Library data...
journal_title:Chinese medical journal
pub_type: 杂志文章,meta分析
doi:10.4103/0366-6999.195464
更新日期:2016-12-20 00:00:00
abstract:OBJECTIVE:To determine whether extraadrenal tissues synthesize aldosterone in addition to vascular tissue and brain. METHODS:Ex vivo kidney perfusion was performed in normal Wistar rats, ACEI pretreated and adrenalectomized rats prior to the perfusion experiment. After equilibration for 30 minutes, 120 ml of perfusate...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1999-05-01 00:00:00
abstract:OBJECTIVE:To summarize the recent findings on the epidemiology of medically important, opportunistic invasive fungal infections (IFIs) in China and discuss the relevant social, economical reasons and medical factors. DATA SOURCES:We performed a comprehensive search of both English and Chinese literatures of opportunis...
journal_title:Chinese medical journal
pub_type: 杂志文章,评审
doi:
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:Intensive treatment such as autologous peripheral blood stem cell (PBSC) transplantation is an important therapeutic strategy in many hematologic malignancies. A number of factors have been reported to impact PBSC mobilization, but the predictive factors varied from one study to another. This retrospective s...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2012-02-01 00:00:00
abstract:BACKGROUND:Laser in situ keratomileusis (LASIK) has become an efficient and commonly performed procedure to reduce refractive errors. In order to further increase the postoperative visual quality, the wavefront-guided refractive surgery has been a research hotspot in customized surgery. This study was conducted to comp...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2008-01-20 00:00:00
abstract:OBJECTIVE:To study the effect of pericardial devascularization combined with Latarjet's innervation on portal hypertension. METHODS:Forty-eight patients undergoing pericardial devascularization combined with Latarjet's innervation were compared with 57 patients with devascularization. Clinical results were evaluated b...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1998-12-01 00:00:00
abstract:BACKGROUND:Hemophilia A (HA) is an X-linked inherited bleeding disorder caused by decreased activity of factor VIII (FVIII) due to heterogenous mutations in the FVIII coding gene (F8). The type of mutation plays an important role in the FVIII inhibitor formation. To date, several studies on the spectra of F8 defects ha...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2010-02-05 00:00:00
abstract:OBJECTIVE:Little information is available to guide treatment strategies regarding patients with unexpected positive cultures (UPC), including Propionibacterium acnes (PA), without overt signs of infection in revision shoulder arthroplasty (RSA). The purpose of our study was to analyze the prevalence, clinical meaning, ...
journal_title:Chinese medical journal
pub_type: 杂志文章,评审
doi:
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:Recurrence or metastasis of myxomas is not rare and can lead to malignancy. We aimed to analyze the risk factors for postoperative cardiac myxoma recurrence and to summarize its clinical characteristics, treatments and classification. METHODS:The clinical data of 5 patients with recurrent cardiac myxoma wer...
journal_title:Chinese medical journal
pub_type: 杂志文章,评审
doi:
更新日期:2012-08-01 00:00:00
abstract::Carbamyl phosphate synthetase I (CPS1) is an initial enzyme of urea synthetase system. It exists exclusively in liver cells and epithelial cells of the small intestine. By immunocytochemistry, 70.5% of 88 surgically resected gastric carcinomas (42 advanced and 46 early gastric carcinomas) was found to be CPS1 immunore...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1989-08-01 00:00:00
abstract:OBJECTIVE:To explore the effects of thyroid hormone (TH) on cardiac function and peripheral lymphocyte beta-adrenoceptors (beta-ARs) of patients with chronic congestive heart failure (CHF). METHODS:Twenty-eight patients with class III or IV advanced CHF due to dilated cardiomyopathy (DCM) or ischemic cardiomyopathy (I...
journal_title:Chinese medical journal
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:2003-11-01 00:00:00
abstract::Dot-ELISA assay was compared with standard Sandwich ELISA in detecting parasite antigen in sera from patients with bancroftian filariasis. The same monoclonal antibody and the same serum samples were used in both assays. With Dot-ELISA, 67 of 70 serum samples from microfilaremic patients were positive at a dilution of...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1990-09-01 00:00:00
abstract:OBJECTIVE:To have a better understanding of genetic contributions to the development of obstructive sleep apnea hypopnea syndrome (OSAHS) by reviewing studies on its genetic basis. DATA SOURCES:A comprehensive search of the PubMed literature without restriction on the publication date was carried out using terms "obst...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:Chronic heart failure (CHF) and diabetes mellitus portend high morbidity and mortality because of an interrelated pathophysiologic process. This large cohort study aimed to analyze the prevalence, clinical characteristics and long-term outcome of patients with CHF and diabetes. METHODS:A total of 1119 patie...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2010-03-20 00:00:00
abstract:BACKGROUND:The earthworm fibrinolytic enzyme (EFE) is a complex protein enzyme that is widely distributed in the earthworm's digestive cavity. Possessing strong protein hydrolysis activity, EFE not only has a direct effect on fibrin, but also can activate plasminogen. Its therapeutic and preventative effects on thrombo...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2007-05-20 00:00:00
abstract:BACKGROUND:Gingerol is the generic term for pungent constituents in ginger, which has been reported to be effective for inhibiting vomiting. We attempted to investigate the antiemetic effect of gingerol and its effective mechanism on substance P and NK(1) receptors in minks. METHODS:The antiemetic effect of gingerol w...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2010-02-20 00:00:00
abstract:BACKGROUND:Urine output (UO) is an essential criterion of the Kidney Disease Improving Global Outcomes (KDIGO) definition and classification system for acute kidney injury (AKI), of which the diagnostic value has not been extensively studied. We aimed to determine whether AKI based on KDIGO UO criteria (KDIGOUO) could ...
journal_title:Chinese medical journal
pub_type: 杂志文章,多中心研究
doi:10.4103/0366-6999.189059
更新日期:2016-09-05 00:00:00
abstract:BACKGROUND:Serum creatinine (Scr) measurement plays a key role in glomerular filtration rate estimation (eGFR), chronic kidney disease (CKD) diagnosis as well as CKD treatment. However, the test results of Scr from different laboratories vary significantly. In order to get comparable results, the European in vitro diag...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2010-09-01 00:00:00
abstract:BACKGROUND:Acute fatty liver of pregnancy (AFLP) is a rare but life-threatening complication occurring in the third trimester. It is often fatal to both mother and fetus. The complicated clinical manifestations as well as an insufficient understanding of the disease make the precise diagnosis and effective treatment of...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.4103/0366-6999.181963
更新日期:2016-05-20 00:00:00
abstract:BACKGROUND:HATCH score is an established predictor of progression from paroxysmal to persistent atrial fibrillation (AF). The purpose of this study was to determine if HATCH score could predict recurrence after catheter ablation of AF. METHODS:The data of 488 consecutive paroxysmal AF patients who underwent an index c...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2012-10-01 00:00:00
abstract:BACKGROUND:The development of the targeted signal transduction inhibitor STI571 has prompted us to treat chronic myeloid leukemia in different ways. Since STI571 may reverse multidrug-resistance of K562/MDR cells in vitro, we studied the effect of STI571 on multidrug-resistant K562 cells in vivo. METHODS:Multidrug-res...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2006-06-05 00:00:00
abstract:BACKGROUND:The SYNergy between percutaneous coronary intervention with TAXus and cardiac surgery Score II (SS-II) can well predict 4-year mortality in patients with complex coronary artery disease (CAD), and guide decision-making between coronary artery bypass graft surgery and percutaneous coronary intervention (PCI)....
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.4103/0366-6999.162510
更新日期:2015-08-20 00:00:00
abstract:BACKGROUND:Double plate osteosynthesis is the standard treatment for intra-articular fractures of the distal humerus. However, there is still controversy concerning dual plate positions in terms of providing optimal stability. The purpose of this retrospective study was to compare the clinical outcomes in patients with...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2013-06-01 00:00:00
abstract:BACKGROUND:Gastric intestinal metaplasia (GIM) is generally considered to be the main mucosal background for the development of gastric adenocarcinomas. Using linked color imaging (LCI), we noticed that the color pattern in areas of GIM was purple mixed with white on the epithelium with signs of mist that were detected...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.1097/CM9.0000000000000172
更新日期:2019-04-05 00:00:00
abstract::Anti-HNK-1 monoclonal antibody (MAb) was reactive with non-cancerous and cancerous prostatic epithelia, as well as natural killer cells, myelinated nerves and cells from APUD systems. The expression of HNK-1 antigen on prostate cancer was investigated immunohistochemically by avidinbiotin-peroxidase complex (ABC) meth...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1995-07-01 00:00:00
abstract:BACKGROUND:The tibial plateau is asymmetric with a larger medial plateau. We observed from clinical practice that the shape of the tibial plateau does not always present a larger medial plateau. Tibial plateau also showed other shapes. The purpose of this study was to analyze the anthropometric data of the proximal tib...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVE:To characterize effects of the GP Ib alpha mutation (A156V) on its interaction with von Willebrand factor (vWf) under high fluid shear stress. METHODS:The residue A156 of GP Ib alpha was converted to a valine and the mutant expressed in CHO cells expressing wild-type GP Ib beta and GPIX. The transfected cell...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2000-08-01 00:00:00