Abstract：
OBJECTIVE:To determine whether extraadrenal tissues synthesize aldosterone in addition to vascular tissue and brain. METHODS:Ex vivo kidney perfusion was performed in normal Wistar rats, ACEI pretreated and adrenalectomized rats prior to the perfusion experiment. After equilibration for 30 minutes, 120 ml of perfusate was collected and subjected to reverse-phase HPLC and then aldosterone was measured by RIA. By RT-PCR and Southern blot the expression of aldosterone synthase gene-CYP11B2 mRNA was studied in both kidney tissue and cultured renal tubular epithelial cell, lung and liver tissues. In situ hybridization was used to identify the cell types of liver and lung expressing CYP11B2 mRNA. RESULTS:Production of aldosterone in the kidney perfusate was not changed in adrenalectomized rats although it was decreased in the group pretreated with ACEI perindopril. By RT-PCR and Southern blot the expression of CYP11B2 mRNA was demonstrated in both kidney tissue and cultured renal tubular epithelial cell. We have also identified CYP11B2 mRNA expression in liver and lung of rats. In situ hybridization showed that CYP11B2 mRNA was localized in the endoplasm of liver fat-storing cell (Ito cells) and type II alveolar cells of lung. CONCLUSIONS:These studies prove that kidney, liver and lung are able to produce aldosterone.
journal_name
Chin Med J (Engl)journal_title
Chinese medical journalauthors
Wu P,Liang X,Dai Y,Liu H,Zang Y,Guo Z,Zhang R,Lai W,Zhang Y,Liu Ykeywords:
subject
Has Abstractpub_date
1999-05-01 00:00:00pages
414-8issue
5eissn
0366-6999issn
2542-5641journal_volume
112pub_type
杂志文章abstract：BACKGROUND:Sinus of Valsalva aneurysm (SVA) is a rare anomaly and few large or long-term series are well established. This study was designed to review 33-year surgical experience of SVA in one center. METHODS:From August 1980 to December 2013, patients with SVA underwent surgical repair were retrospectively studied. ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
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pub_type： 杂志文章
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journal_title：Chinese medical journal
pub_type： 杂志文章,随机对照试验
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abstract：:Lymphocytes from regional lymph nodes of patients with ovarian carcinoma were immortalized by fusing them with a nonsecreting cell line of murine myeloma (Sp2/0-Ag14). The fusion rate was 0-87.5%. By early cloning and recloning, a hybrid cell line, named HMD4, was established. It has stably secreted human IgG for more...
journal_title：Chinese medical journal
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journal_title：Chinese medical journal
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doi：
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journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
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journal_title：Chinese medical journal
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journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2004-06-01 00:00:00
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journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2001-10-01 00:00:00
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journal_title：Chinese medical journal
pub_type： 杂志文章,评审
doi：10.4103/0366-6999.241805
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journal_title：Chinese medical journal
pub_type： 杂志文章,评审
doi：
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journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
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journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2009-04-20 00:00:00
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journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
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journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2009-11-20 00:00:00
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journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2004-02-01 00:00:00
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journal_title：Chinese medical journal
pub_type： 杂志文章,评审
doi：
更新日期：2011-11-01 00:00:00
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journal_title：Chinese medical journal
pub_type： 杂志文章,评审
doi：
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journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
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journal_title：Chinese medical journal
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journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
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更新日期：2011-12-01 00:00:00
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journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.4103/0366-6999.192782
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pub_type： 杂志文章
doi：
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journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
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journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
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journal_title：Chinese medical journal
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