Abstract：
BACKGROUND:Ilexonin A (IA), purified from the Chinese herbal medicine Maodongqing (Ilex pubescens Hook, et Arn) has been commonly used in south China to treat thrombotic disorders. In this study, we aimed to study the inhibiting effects and mechanism of IA on von Willebrand factor (vWF)-dependent high shear-induced platelet aggregation. METHODS:vWF-dependent high shear (10,800 s(-1)) induced aggregation of platelets obtained from normal donors in the presence or absence of IA was measured by a modified cone-plate viscometer and shear-induced vWF binding was measured by quantitative flow cytometry with monoclonal antibody known to bind exclusively to the C-terminal domain of vWF (LJ-C3) directly labeled with fluorescein isothiocyanate (FITC). P-selectin surface expression was also measured by a similar method with FITC conjugated anti-P-selectin monoclonal antibody (WGA1). RESULTS:Shear-induced platelet aggregation was inhibited by IA in a dose-dependent manner. The extent of aggregation decreased from (78.6 +/- 4.6)% in the absence of IA to (36.5 +/- 2.1)% in the presence of IA (3.3 mmol/L) (P < 0.0001, n = 9) with a high shear rate of 10800 s(-1). vWF binding and P-selectin expression were also inhibited by IA in a dose dependent manner. The number of binding FITC-LJ-C3 molecules increased after exposure of platelet-rich plasma to a high shear rate of 10800 s(-1) for 6 minutes, but this shear-induced increased binding platelet surface vWF molecules and P-selectin expression can be decreased in the presence of IA. CONCLUSION:vWF binding and vWF mediated platelet activation, aggregation occurring under high shear rate were inhibited by IA. IA may be a unique antithrombotic drug inhibiting the vWF-GP Ibalpha interaction, and may thus facilitate drug design targeting arterial thrombosis.
journal_name
Chin Med J (Engl)journal_title
Chinese medical journalauthors
Li M,Wu WK,Liu L,Liao FL,Shinohara Y,Handa S,Goto Skeywords:
subject
Has Abstractpub_date
2004-02-01 00:00:00pages
241-6issue
2eissn
0366-6999issn
2542-5641journal_volume
117pub_type
杂志文章abstract：BACKGROUND:Thoracic endovascular aortic repair (TEVAR) is an emerging treatment modality, which has been rapidly embraced by clinicians treating thoracic aortic disease. However, the clinical manifestations of systemic inflammatory response after TEVAR as post-implantation syndrome (PIS) resemble the perioperative infe...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
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abstract：OBJECTIVE:To investigate the correlation among retinal fluorescein angiography, blood viscosity, and other factors in patients with open angle glaucoma (POAG). METHODS:Multiple step regression analysis was made to investigate the correlation between each of the following blood vessel filling times: the arm-choroid (A-...
journal_title：Chinese medical journal
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更新日期：1997-09-01 00:00:00
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journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
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abstract：BACKGROUND:Previous studies have showed that photooxidative stress can lead to down-modulation of nuclear factor-kappa B (NF-kappaB) activity causing apoptosis of cultured photoreceptor cells. This study aimed at investigating whether NF-kappaB was involved in photoreceptor cells apoptosis induced by N-methyl-N-nitroso...
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pub_type： 杂志文章,meta分析,评审
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abstract：BACKGROUND:Although the use of hepatitis B immunoglobulin (HBIG) may lead to a significant reduction in recurrent hepatitis B virus (HBV) infection and improve the survival of patients who have undergone liver transplantation (LT) for hepatitis B-related diseases, the recurrence of the disease still remains at a lower ...
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journal_title：Chinese medical journal
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pub_type： 杂志文章,多中心研究
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journal_title：Chinese medical journal
pub_type： 杂志文章,评审
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pub_type： 杂志文章
doi：
更新日期：2003-01-01 00:00:00
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doi：
更新日期：2014-01-01 00:00:00
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doi：
更新日期：2007-12-05 00:00:00
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pub_type： 杂志文章
doi：10.1097/CM9.0000000000000544
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doi：
更新日期：2012-03-01 00:00:00
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doi：
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doi：
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pub_type： 杂志文章
doi：
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