Abstract：
OBJECTIVE:To investigate the feasibility and features of 3 catheter-mediated approaches of gene transfer into heart, including direct myocardial injection (DMI), coronary artery perfusion (CAP), and intrapericardial cavity injection (ICI). METHODS:Fifteen dogs were used, and 0.3 ml (1 x 10(9) pfu) of an adenovirus (Adex1SR LacZ) was injected into the heart by 3 methods. The dogs were killed 5 days following injection, and gene expressions in heart and liver were evaluated by histochemical analysis. RESULTS:The results showed that (1) the CAP method was relatively less damaging and induced sparse LacZ expression in the myocardium, and the gene expression was also found in both vessels within the myocardium and liver; (2) gene transfer by DMI resulted in intense LacZ expression around the injection accompanied by a local inflammatory response; (3) LacZ expression elicited by ICI was detected in either the inner surface of the parietal pericardium or epicardial surface of the heart, and also in the myocardium underlying the visceral pericardium. CONCLUSION:Three catheter-mediated methods of gene transfer into the heart may be used and a reasonable approach should be chosen according to purpose.
journal_name
Chin Med J (Engl)journal_title
Chinese medical journalauthors
Li J,Li G,Huang C,Jiang H,Tang Q,Xu J,Xia H,Wang Jkeywords:
subject
Has Abstractpub_date
2002-04-01 00:00:00pages
612-3issue
4eissn
0366-6999issn
2542-5641journal_volume
115pub_type
杂志文章abstract：OBJECTIVE:To rapidly and economically obtain knowledge about adult stage Schistosoma japonicum (Chinese strain) expressed genes using expressed sequence tag (EST). METHODS:A directional cDNA library constructed from Schistosoma japonicum (Chinese strain) adult stage RNA was used to generate expressed sequence tags (ES...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2002-10-01 00:00:00
abstract：BACKGROUND:Angiogenesis and lymphogenesis which were promoted by vascular endothelial growth factor (VEGF) and VEGF-C are important in the growth and metastasis of solid tumors. The high level of VEGF and VEGF-C were distributed in numerous types of cancers, but their distribution and expression in Wilms tumor, the mos...
journal_title：Chinese medical journal
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doi：
更新日期：2011-11-01 00:00:00
abstract：OBJECTIVE:To clarify the role of these cyclins in human gastric cancer. METHODS:38 gastric cancer patients, 29 first degree relatives of gastric cancer patients, as well as 18 healthy subjects were included. The mRNA expression of cyclins D1, D2, D3 and E in gastric biopsies was evaluated by RT-PCR analysis using spec...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2002-05-01 00:00:00
abstract：BACKGROUND:Jaw osteonecrosis possibly associated with the administration of bisphosphonates is expected to be treated with a non-pharmacologic approach. This study aimed to determine whether noninvasive, mechanically mediated vibration would inhibit the decline in bone mineral density (BMD) that follows menopause, enha...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2008-07-05 00:00:00
abstract：OBJECTIVE:The P53 tumor suppressor gene shows the most frequent genetic alteration in human tumors. Mutation, deletion and rearrangement of P53 gene have been found in several types of neoplasm including osteosarcoma. The present study is to clarify the status of abnormalities of this gene in osteosarcoma in China. MA...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1996-10-01 00:00:00
abstract：BACKGROUND:Orthostatic intolerance (OI) is a common disease at pediatric period which has a serious impact on physical and mental health of children. The purpose of this study was to investigate the effect of related factors on the prognosis of children with OI. METHODS:The subjects were 170 children with OI, includin...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
abstract：BACKGROUND:Some esophageal cancer patients complicated with depression exhibit cognitive impairments. Frontal electroencephalogram (EEG) may be used as a reliable biomarker for prefrontal-mediated cognitive functions. This study was to investigate alterations of EEG and frontal cognitive impairment in esophageal cancer...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.4103/0366-6999.211552
更新日期：2017-08-05 00:00:00
abstract：:The unclear DNA content was measured with flow cytometry (FCM) in 167 specimens of normal nasopharyngeal epithelium (NE), adjacent tissue to carcinoma (ATC) and nasopharyngeal carcinoma (NPC). All 20 patients with NE showed diploid, whereas 38 (38%) of 101 patients with NPC, 7 (58%) of 12 patients with recurrent NPC, ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1994-05-01 00:00:00
abstract：OBJECTIVE:To investigate the relationship between angiotensin-converting enzyme (ACE) gene insertion/deletion polymorphism and the clinico-pathological manifestations in patients with immunoglobulin nephropathy (IgAN). METHODS:A flanking primer pair and an insertion-specific primer pair were used to perform two polyme...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1997-07-01 00:00:00
abstract：BACKGROUND:Acute rejection is one of the most important factors for prognosis following liver transplantation. With the use of potent immunosuppressants, acute rejection does not always present typical manifestations. Moreover, other complications often occur concomitantly after liver transplantation, which makes early...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2009-06-20 00:00:00
abstract：BACKGROUND:The effects of near-road pollution on lung function in China have not been well studied. We aimed to investigate the effects of long-term exposure to traffic-related air pollution on lung function, airway inflammation, and respiratory symptoms. METHODS:We enrolled 1003 residents aged 57.96 ± 8.99 years livi...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.4103/0366-6999.189923
更新日期：2016-09-20 00:00:00
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journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2001-03-01 00:00:00
abstract：BACKGROUND:To compare the efficacy and safety of hormone replacement therapy (HRT) combined with fluoxetine, with HRT alone, in post-menopausal women suffering from depression. METHODS:A randomized, open-label, parallel trial was applied. HRT was administered to all patients for 2 cycles, with 14 days of estrogen ther...
journal_title：Chinese medical journal
pub_type： 临床试验,杂志文章,随机对照试验
doi：
更新日期：2004-02-01 00:00:00
abstract：:Enhanced recovery after cesarean (ERAC) delivery is an evidence-based, multi-disciplinary approach throughout pre-, intra-, post-operative period. The ultimate goal of ERAC is to enhance recovery and improve the maternal and neonatal outcomes. This review highlights the role of anesthesiologist in ERAC protocols. This...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.1097/CM9.0000000000000644
更新日期：2020-03-05 00:00:00
abstract：BACKGROUND:Hemolysis-elevated liver enzymes-low platelet counts (HELLP) syndrome is a clinical condition occurring in middle and late stage pregnancy. It is characterized by hemolysis, elevated liver enzymes and low platelet counts. This study involves the analysis of the diagnosis, clinical characteristics and treatme...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2010-05-20 00:00:00
abstract：BACKGROUND:Hepatocyte growth factor (HGF) treats ischemic disease by promoting arteriogenesis, however, its mechanism of action is not known. The notch signaling pathway plays an important role in neovascularization. The relationship between the proliferation and migration ability of artery endothelial cells and the Dl...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2011-01-01 00:00:00
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journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2012-10-01 00:00:00
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journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2011-03-01 00:00:00
abstract：OBJECTIVE:To characterize effects of the GP Ib alpha mutation (A156V) on its interaction with von Willebrand factor (vWf) under high fluid shear stress. METHODS:The residue A156 of GP Ib alpha was converted to a valine and the mutant expressed in CHO cells expressing wild-type GP Ib beta and GPIX. The transfected cell...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2000-08-01 00:00:00
abstract：OBJECTIVE:To study the severe acute respiratory syndrome (SARS)-associated coronavirus genotype and its characteristics. METHODS:A SARS-associated coronavirus isolate named ZJ01 was obtained from throat swab samples taken from a patient in Hangzhou, Zhejing province. The complete genome sequence of ZJ01 consisted of 2...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2003-09-01 00:00:00
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journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2003-08-01 00:00:00
abstract：BACKGROUND:This study evaluated the prognostic impact of D2 lymphadenectomy combined with splenectomy in patients with advanced proximal gastric cancer and lymph node metastasis at the splenic hilum (No. 10 lymph nodes). METHODS:The clinical records of 216 patients with advanced proximal gastric cancer and No. 10 lymp...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2009-11-20 00:00:00
abstract：BACKGROUND:The earthworm fibrinolytic enzyme (EFE) is a complex protein enzyme that is widely distributed in the earthworm's digestive cavity. Possessing strong protein hydrolysis activity, EFE not only has a direct effect on fibrin, but also can activate plasminogen. Its therapeutic and preventative effects on thrombo...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2007-05-20 00:00:00
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journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2000-04-01 00:00:00
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journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2003-09-01 00:00:00
abstract：BACKGROUND:Apoptosis is a major cause of ischemic heart dysfunction. Apelin, the endogenous ligand for the G-protein-coupled APJ receptor, has been reported to exert cardioprotective effects during myocardial injury. The aim of this study was to investigate the effects of apelin on apoptosis of rat cardiomyocytes induc...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2009-10-05 00:00:00
abstract：OBJECTIVE:To investigate the short- and long-term clinician and angiographic outcome of stenting or angioplasty for chronic total coronary artery occlusion. METHODS:A total of 95 patients with successful percutaneous transluminal coronary angioplasty (PTCA) for total occlusion coronary artery were identified out of 35...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2000-03-01 00:00:00
abstract：BACKGROUND:Zika virus (ZIKV) has emerged as a global pathogen causing significant public health concerns. China has reported several imported cases where ZIKV were carried by travelers who frequently travel between China and ZIKV-endemic regions. To fully characterize the ZIKV strains isolated from the cases reported i...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.1097/CM9.0000000000000317
更新日期：2019-07-20 00:00:00
abstract：BACKGROUND:Gastric varices (GV) are life-threatening for patients with portal hypertension. Endoscopic injection with butyl cyanoacrylate (BC), the mainstay of the therapy for GV, has been reported to be effective for hemostasis of bleeding varices, but its efficacy in the obliteration of GV and impact on the survival ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2007-12-05 00:00:00
abstract：BACKGROUND:Novel influenza A viruses of avian-origin may be the precursors of pandemic strains. This descriptive study aims to introduce a novel avian-origin influenza A (H10N8) virus which can infect humans and cause severe diseases. METHODS:Collecting clinical data of three cases of human infection with a novel reas...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
