Abstract:
BACKGROUND:Hepatocyte growth factor (HGF) treats ischemic disease by promoting arteriogenesis, however, its mechanism of action is not known. The notch signaling pathway plays an important role in neovascularization. The relationship between the proliferation and migration ability of artery endothelial cells and the Dll4-Notch-Hey2 signaling pathway in the process of arteriogenesis was investigated as a mechanism of action of HGF. METHODS:Based on the prophase study cells and supernatant were harvested at the indicated time after human femoral artery endothelial cells (HFAECs) were infected with adenovirus-HGF (Ad-HGF) at 200 pfu/cell. Cells were analyzed for HGF expression and Notch1, Dll4 and Hey2 expression by ELISA and reverse transcription-PCR (RT-PCR). The changes in the proliferation and migration ability of HFAECs were observed by MTT and Transwell migration experiments. Ad-GFP-infected HFAECs were used as control. RESULTS:Compared with the control group the Ad-HGF group's HGF expression was not increased with time, and the induction by HGF of Notch1, Dll4 and Hey2 gene transcription was not enhanced with an increase of HGF. The proliferation ability of Ad-HGF-transduced HFAECs was enhanced and their migration ability was also enhanced in the presence of HGF. CONCLUSIONS:Through activating the Dll4-Notch-Hey2 signaling pathway, HGF indirectly promotes the proliferation and migration ability of cells, so that offspring artery branches are formed.
journal_name
Chin Med J (Engl)journal_title
Chinese medical journalauthors
Gao YF,Ha XQ,Lü TD,Han JPsubject
Has Abstractpub_date
2011-01-01 00:00:00pages
127-31issue
1eissn
0366-6999issn
2542-5641journal_volume
124pub_type
杂志文章abstract::The effects of low-dose estrogen and progestogen on menopausal symptoms were studied with Kuppermen score and urinary excretion of calcium as fasting morning urine Ca/Cr ratio in 69 perimenopausal women. The subjects were divided into 3 groups: amenorrhea less than 1 year (14 women); post menopause 1-3 years (19); and...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1990-08-01 00:00:00
abstract:BACKGROUND:Embryo quality and receptivity of the endometrium are two factors that determine the results of in vitro fertilization/intra-cytoplasmic sperm injection-embryo transfer (IVF/ICSI-ET). There is no consensus of the optimal transfer strategy for normal responders or high responders. The current study aimed to f...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2013-01-01 00:00:00
abstract::Since February 1982, 23 patients with scoliosis were treated by releasing the soft tissues on the concave side and plaster spinal fusion jacket. Of these patients, 13 had congenital scoliosis and 10 idiopathic scoliosis. Follow-up for 1 to 8.5 years showed that the results of correction were satisfactory. The maximum ...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1992-02-01 00:00:00
abstract:BACKGROUND:Now lung volume reduction surgery (LVRS) has become one of the most effective methods for the management of some cases of severe chronic obstructive pulmonary disease (COPD). We evaluated the mid-term effects of LVRS on pulmonary function in patients with severe COPD. METHODS:Ten male patients with severe C...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2007-04-20 00:00:00
abstract:OBJECTIVE:This article aims to present a brief profile of the advances in prevention and treatment of myocardial injury in cancer therapy based on relevant literature or reports. DATA SOURCES:The data cited in this review were obtained from articles indexed in PubMed and China National Knowledge Internet (CNKI) up to ...
journal_title:Chinese medical journal
pub_type: 杂志文章,评审
doi:10.1097/CM9.0000000000000498
更新日期:2019-11-20 00:00:00
abstract::Carbamyl phosphate synthetase I (CPS1) is an initial enzyme of urea synthetase system. It exists exclusively in liver cells and epithelial cells of the small intestine. By immunocytochemistry, 70.5% of 88 surgically resected gastric carcinomas (42 advanced and 46 early gastric carcinomas) was found to be CPS1 immunore...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1989-08-01 00:00:00
abstract:BACKGROUND:Many recipients of implantable cardiac electronic devices have atrial fibrillation (AF) occurrences after device implantation, even if there is no previous history of AF, and some of the episodes are asymptomatic. The purpose of this study was to evaluate trends in AF burden following early AF detection in p...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2013-11-01 00:00:00
abstract:BACKGROUND:Spinal cord injury (SCI) is a worldwide medical concern. This study aimed to elucidate the mechanism underlying the protective effect of hyperbaric oxygen (HBO) against SCI-induced neurologic defects in rats via exploring the stromal cell-derived factor-1 (SDF-1)/CXC chemokine receptor 4 (CXCR4) axis and exp...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.1097/CM9.0000000000000115
更新日期:2019-03-20 00:00:00
abstract::Human keratinocytes in primary culture stimulated by Ca2+ ionophore A23187(Io) could synthesize and release a material which might aggregate aspirin-treated washed rabbit platelets and was identified as platelet activating factor (PAF) by four methods. Io stimulated the production of PAF by keratinocytes in a time- an...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1994-05-01 00:00:00
abstract:OBJECTIVE:To study the effect of pericardial devascularization combined with Latarjet's innervation on portal hypertension. METHODS:Forty-eight patients undergoing pericardial devascularization combined with Latarjet's innervation were compared with 57 patients with devascularization. Clinical results were evaluated b...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1998-12-01 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.4103/0366-6999.152664
更新日期:2015-03-20 00:00:00
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journal_title:Chinese medical journal
pub_type: 临床试验,杂志文章
doi:
更新日期:2013-07-01 00:00:00
abstract:BACKGROUND:Assessment of perfusion in the early period of acute renal failure (ARF) is important, and can not be made by conventional ultrasound. The aim of this study was to prospectively test in a rabbit ARF model whether real-time contrast-enhanced ultrasound (CEUS) can quantitatively evaluate the hemodynamic change...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2012-02-01 00:00:00
abstract:BACKGROUND:Insulin-like growth factor-II mRNA-binding protein 3 (IMP3) is a newly identified mRNA-binding protein that is involved in embryogenesis and carcinogenesis of some malignant tumors. The aim of this study was to detect the expression of IMP3 protein in gastric adenocarcinoma (GAC) and the correlation with cli...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2010-12-01 00:00:00
abstract:OBJECTIVE:To detect occult metastases in lymph nodes from patients with colorectal carcinoma and its clinical significance. METHODS:The metastases in 260 lymph nodes from 39 histologically verified colorectal cancer patients were studied by both hematoxylin-eosin (HE) staining and cytokeratin-20 (CK20) specific RT-PCR...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2002-04-01 00:00:00
abstract:: ...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.4103/0366-6999.244110
更新日期:2018-11-05 00:00:00
abstract:PURPOSE:To review the current status of multimodality treatment and lymphadenectomy in the management of esophageal cancer. DATA SOURCES:Literature review. STUDY SELECTION:Multimodality treatment and lymphadenectomy in esophageal cancer. DATA EXTRACTION:Results in research papers published selected by literature sea...
journal_title:Chinese medical journal
pub_type: 杂志文章,评审
doi:
更新日期:1997-11-01 00:00:00
abstract:BACKGROUND:While combined oral contraceptives (COCs) are commonly used to treat polycystic ovary syndrome (PCOS), comparative data regarding metabolic effects of different progestogens on this patient population are missing. This study aimed to compare the different effects of drospirenone (DRP)-containing COCs with cy...
journal_title:Chinese medical journal
pub_type: 杂志文章,随机对照试验
doi:10.4103/0366-6999.179783
更新日期:2016-04-20 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1998-04-01 00:00:00
abstract:BACKGROUND:Matrix metalloproteinase (MMP)-2 plays an important role in the remodeling of left ventricles (LVs) and right ventricles (RVs). We investigated the differences of MMP-2 expression between LV and RV in response to nandrolone decanoate (ND), swimming training (ST), and combined ND and ST (NS) in mice, based on...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.4103/0366-6999.222330
更新日期:2018-01-20 00:00:00
abstract:BACKGROUND:Subcapital femoral neck fracture in young adults has many complications, and the incidence is increasing year-by-year. The selection of the proper operation method to avoid them is an ambiguous matter. This study aimed to evaluate the treatment effect of subcapital femoral neck fracture by the capsulotomy an...
journal_title:Chinese medical journal
pub_type: 杂志文章,随机对照试验
doi:10.4103/0366-6999.151092
更新日期:2015-02-20 00:00:00
abstract:BACKGROUND:Acinetobacter baumannii is one of the main gram-negative bacilli in clinical practice. Nosocomial infections caused by multi-drug resistance Acinetobacter baumannii is very difficult to treat. This study was designed to investigate the antimicrobial resistance characteristics and four resistant gene expressi...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2005-01-20 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2010-09-01 00:00:00
abstract:BACKGROUND:Endothelial cells derived microRNAs can be detected in plasma and serum and there is evidence that inflammatory disease states may affect the levels of circulating microRNAs. However, there is no direct proof that inflammation induces endothelial cells to release microRNAs into circulation. This study aimed ...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
abstract::Four calcium channel blockers, i.e. nifedipine, verapamil, cinnarizine and tetrandrine are currently available and used widely in treating cardiovascular diseases. To confirm the effects, if any, of calcium channel blockers on cirrhotic patients with portal hypertension, a study was performed on esophageal variceal pr...
journal_title:Chinese medical journal
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:1995-11-01 00:00:00
abstract:OBJECTIVE:To report our experiences in retroperitoneoscopic renal pedicle lymphatic stripping for chyluria. METHODS:Six cases of filarial chyluria were admitted to our hospital from November 2001 to June 2002. Of these cases, 4 were men and 2 women, with age ranging from 34 to 52 years (mean, 42 years). Diagnosis was ...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2003-11-01 00:00:00
abstract:OBJECTIVE:To explore the mechanism of cisplatin resistance and its reversion in human ovarian cancer. MATERIALS AND METHODS:A cisplatin resistant cell subline of SKOV3, SKOV3/cp, was established, and a xenograft mice model of human ovarian cancer was established by microencapsulation technology. Various biochemical ch...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1996-05-01 00:00:00
abstract:BACKGROUND:Sinus of Valsalva aneurysm (SVA) is a rare cardiac anomaly, and SVA with discrete membranous subaortic stenosis is even rarer. The aim of the study was to make sure the incidence of SVA with discrete membraneous subaortic stenosis in SVA and their surgical results. We retrospectively analyzed 234 patients re...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2012-05-01 00:00:00
abstract:BACKGROUND:Some esophageal cancer patients complicated with depression exhibit cognitive impairments. Frontal electroencephalogram (EEG) may be used as a reliable biomarker for prefrontal-mediated cognitive functions. This study was to investigate alterations of EEG and frontal cognitive impairment in esophageal cancer...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.4103/0366-6999.211552
更新日期:2017-08-05 00:00:00
abstract:OBJECTIVE:To evaluate the long-term efficacy and safety of lamivudine therapy for the treatment of chronic hepatitis B and the clinical influence of emergence of tyrosine methionine aspartic acid (YMDD) motif mutation of hepatitis B virus (HBV). METHODS:This multicenter, double-blind, randomized, placebo controlled tr...
journal_title:Chinese medical journal
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:
更新日期:2002-12-01 00:00:00