Abstract:
BACKGROUND:Green tea has been shown to improve cholesterol metabolism in animal studies, but the molecular mechanisms underlying this function have not been fully understood. Long non-coding RNAs (lncRNAs) have recently emerged as a major class of regulatory molecules involved in a broad range of biological processes and complex diseases. Our aim was to identify important lncRNAs that might play an important role in contributing to the benefits of epigallocatechin-3-gallate (EGCG) on cholesterol metabolism. METHODS:Microarrays was used to reveal the lncRNA and mRNA profiles in green tea polyphenol(-)-epigallocatechin gallate in cultured human liver (HepG2) hepatocytes treated with EGCG and bioinformatic analyses of the predicted target genes were performed to identify lncRNA-mRNA targeting relationships. RNA interference was used to investigate the role of lncRNAs in cholesterol metabolism. RESULTS:The expression levels of 15 genes related to cholesterol metabolism and 285 lncRNAs were changed by EGCG treatment. Bioinformatic analysis found five matched lncRNA-mRNA pairs for five differentially expressed lncRNAs and four differentially expressed mRNA. In particular, the lncRNA AT102202 and its potential targets mRNA-3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR) were identified. Using a real-time polymerase chain reaction technique, we confirmed that EGCG down-regulated mRNA expression level of the HMGCR and up-regulated expression of AT102202. After AT102202 knockdown in HepG2, we observed that the level of HMGCR expression was significantly increased relative to the scrambled small interfering RNA control (P < 0.05). CONCLUSIONS:Our results indicated that EGCG improved cholesterol metabolism and meanwhile changed the lncRNAs expression profile in HepG2 cells. LncRNAs may play an important role in the cholesterol metabolism.
journal_name
Chin Med J (Engl)journal_title
Chinese medical journalauthors
Liu G,Zheng X,Xu Y,Lu J,Chen J,Huang Xdoi
10.4103/0366-6999.147824subject
Has Abstractpub_date
2015-01-05 00:00:00pages
91-7issue
1eissn
0366-6999issn
2542-5641pii
ChinMedJ_2015_128_1_91_147824journal_volume
128pub_type
杂志文章abstract:OBJECTIVE:Clinical application of autologous fat grafting (AFG) is quickly expanding. Despite the widely acceptance, long-term survival rate (SR) of AFG remains a question not yet solved. Meanwhile, although rare, severe complications related to AFG including vision loss, stroke even death could be seen in the literatu...
journal_title:Chinese medical journal
pub_type: 杂志文章,评审
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journal_title:Chinese medical journal
pub_type: 杂志文章
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journal_title:Chinese medical journal
pub_type: 杂志文章
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更新日期:1993-06-01 00:00:00
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journal_title:Chinese medical journal
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doi:
更新日期:2007-04-05 00:00:00
abstract::Selective ablation of slow pathway by radiofrequency (RF) current in 34 patients with atrioventricular (AV) node pathways and tachycardia was performed with two methods in our section; posterior approach was used in the first 12 patients, the slow pathways were ablated successfully. Inferior approach was performed in ...
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更新日期:2009-04-20 00:00:00
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更新日期:2012-05-01 00:00:00
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更新日期:2007-04-20 00:00:00
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journal_title:Chinese medical journal
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更新日期:2013-11-01 00:00:00
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doi:
更新日期:2004-05-01 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2011-07-05 00:00:00
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pub_type: 杂志文章
doi:
更新日期:2008-01-05 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2010-11-01 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.1097/CM9.0000000000000709
更新日期:2020-04-05 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2007-08-05 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract::100 women ranging in age from 26-34 years were followed up for 4-21 months after undergoing sterilization by tubal occlusion using intraluminal threads and silver clips. The number of children/woman ranged from 1-5 with 80% having 2. 31 women underwent sterilization postmenstrually, 11 midmenstrually, 28 premenstrua...
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doi:
更新日期:1983-08-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2015-07-20 00:00:00
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doi:
更新日期:2007-10-05 00:00:00
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更新日期:2010-07-01 00:00:00
abstract:BACKGROUND:Limited data exist on the association of serum gamma-glutamyl transferase (GGT) level within the reference range with the increased risk of coronary heart disease (CHD) prediction in men. The study examined the association between serum GGT concentration within the reference range and the CHD risk prediction...
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更新日期:2015-08-05 00:00:00
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doi:
更新日期:2014-01-01 00:00:00
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doi:
更新日期:2010-09-01 00:00:00
abstract:BACKGROUND:Few clinical studies or randomized clinical trial results have reported the impact of fast track surgery on postoperative insulin sensitivity. This study aimed to investigate the effects of fast track surgery on postoperative insulin sensitivity in patients undergoing elective open colorectal resection. MET...
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pub_type: 杂志文章,随机对照试验
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更新日期:2010-01-20 00:00:00
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