Abstract：
BACKGROUND:The coordinated change of haematopoietic supporting microenvironment in bone marrow (BM) is crucial for innate immunity and inflammation. As the precursors of marrow stroma, BM derived mesenchymal stem cells (MSCs) promote haematopoietic function, but their roles in innate immunity or inflammation have not been investigated. Here we investigated the expression of Toll like receptor 4 (TLR-4) and the effect of lipopolysaccharide (LPS) on its expression in BM MSCs in vitro. METHODS:MSCs were harvested from adult rat's BM cells by density gradient centrifugation and adhesive culture. The purity of MSCs were identified with the cell morphological feature and osteogenic capacity, the phenotypes were tested by flow cytometry. Cultured MSCs were treated by LPS (1 microg/ml, 10 microg/ml or 100 microg/ml) for 24 hours. The relative expression levels of TLR-4 mRNA were detected by semiquantitative reverse transcription polymerase chain reaction and costimulatory molecules (CD80, CD86 and MHC-II) expressed on MSCs were analyzed by flow cytometry. The levels of tumor necrosis factor-alpha (TNF-alpha) in supernatants were determined by enzyme linked immunosorbent assay. RESULTS:After incubation with LPS, MSCs expressed the higher levels of TLR-4 mRNA, costimulatory molecules and TNF-alpha than the untreated group: LPS 10 microg/ml was the most effective (P < 0.01); the levels of TLR-4 mRNA, costimulatory molecules and TNF-alpha decreased when MSCs were exposed to 100 microg/ml LPS. Except for MHC-II and TNF-alpha (P > 0.05), the levels of CD80, CD86 and TLR-4 mRNA were significantly lower than that in the treated group of 10 microg/ml (P < 0.01). CONCLUSION:MSCs expressed TLR-4 mRNA. LPS activated the functional expression levels of TLR-4 in MSCs although the activity may depend on the concentration of LPS.
journal_name
Chin Med J (Engl)journal_title
Chinese medical journalauthors
Shi L,Wang JS,Liu XM,Hu XY,Fang Qsubject
Has Abstractpub_date
2007-10-05 00:00:00pages
1685-8issue
19eissn
0366-6999issn
2542-5641journal_volume
120pub_type
杂志文章abstract：BACKGROUND:The reports on massive transfusions (MTs) in obstetrics have recently been an increasing trend. We aimed to define the clinical features, risk factors, main causes, and outcomes of MTs due to severe postpartum hemorrhage (PPH) and the frequency trends over the past 10 years. METHODS:We retrospectively analy...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.4103/0366-6999.200545
更新日期：2017-03-05 00:00:00
abstract：BACKGROUND:The In1.1C single nucleotide polymorphism (SNP) allele results in reduced RANTES transcription, which is associated with increased frequency of HIV-1 infection, and rapid progression to AIDS among HIV-1-infected individuals. This study aimed to study the mutant frequency and polymorphism of RANTES in Chinese...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2009-05-20 00:00:00
abstract：BACKGROUND:Hippophae rhamnoides L. (HL) exerts antioxidant activities against various oxidative stress conditions. In this study, we investigated effects of extract from HL leaves (HLE) on cell proliferation and neuroblast differentiation in the subgranular zone (SGZ) of the dentate gyrus (DG) of aged gerbils. METHODS...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
abstract：:We presented the experimental results of endothelial cells (EC) seeding in small caliber interpositional dacron grafts of canine carotid arteries. Six weeks after surgery, 9 (56.25%) seeded, and 6 (37.5%) control grafts remained patent. The mean thrombus-free surface area was 83.4% in the seeded and 62% in the control...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1989-08-01 00:00:00
abstract：:A patient with both a femoral attachment injury (peel-off injury) and musculotendinous junction avulsion of the popliteus, a so-called “floating popliteus tendon injury”, received arthroscopy-assisted popliteus reconstruction. The injured ligaments were addressed in the same procedure, including mini-open direct repai...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2011-12-01 00:00:00
abstract：:One 22-month-old boy who was admitted for a fever lasting 6 days as well as a cough and wheezing lasting 2 days was reported. He was diagnosed with influenza A (H1N1, severe type), severe pneumonia, acute respiratory distress syndrome (ARDS), Evans syndrome and multiple organ failure. This is the first case of novel i...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2011-06-01 00:00:00
abstract：BACKGROUND:Atrial fibrillation (AF) is the most common supraventricular arrhythmia in clinical practice. Chronic atrial fibrillation (CAF) is associated with ionic remodeling. However, little is known about the activity of ATP-sensitive potassium current (IK, ATP) during CAF. So we studied the changes of IK, ATP densit...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2005-07-20 00:00:00
abstract：OBJECTIVE:To explore the mechanism of autoimmune hemolysis and establish a more sensitive test for autoimmune hemolytic anemia (AIHA). METHODS:IgG subclasses were tested in 40 patients with idiopathic or secondary AIHA by Coombs test with monoclonal antibodies. Hb, TBil, RC and FHb, as hemolytic parameters, were used ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1999-09-01 00:00:00
abstract：BACKGROUND:Airway mucus hypersecretion is an important pathophysiological feature of chronic obstructive pulmonary disease, which is closely associated with cigarette smoking. However, the signal transduction pathway from the cell surface to the nucleus through which cigarette smoke causes upregulation of mucin gene ex...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2011-10-01 00:00:00
abstract：BACKGROUND:Nasopharyngeal carcinoma (NPC) is a squamous-cell carcinoma especially prevailing among the natives of southern China. The regimen of concurrent chemoradiotherapy (CCRT) that include platinum and 5-fluorouracil (5-FU) is considered to be the standard treatment for NPC. However, its clinical use is limited by...
journal_title：Chinese medical journal
pub_type： 杂志文章,meta分析
doi：
更新日期：2014-01-01 00:00:00
abstract：BACKGROUND:Cholesterol-lowering therapy with statins has been reported to reduce the morbidity and mortality of cardiovascular diseases. This study aimed to investigate the effects of combined application of extended-release niacin and atorvastatin on lipid profile modification and the risks of adverse events in patien...
journal_title：Chinese medical journal
pub_type： 杂志文章,随机对照试验
doi：
更新日期：2009-07-20 00:00:00
abstract：BACKGROUND:Postoperative preablative stimulated thyroglobulin (ps-Tg) has been evaluated in predicting prognosis and success of ablation regarding differentiated thyroid cancer (DTC); however, its relationship with recurrence risk and radioiodine decision-making remains uncertain, especially in Chinese DTC patients. We...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.4103/0366-6999.155086
更新日期：2015-04-20 00:00:00
abstract：BACKGROUND:The optimal surgical approach for four-level cervical spondylotic myelopathy remains controversial. The purpose of this study was to compare clinical and radiological outcomes and complications between the anterior and posterior approaches for four-level cervical spondylotic myelopathy. METHODS:A total of 1...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.1097/CM9.0000000000001146
更新日期：2020-12-05 00:00:00
abstract：BACKGROUND:One of the major causes of death in severe acute pancreatitis (SAP) is severe infection owing to bacterial translocation. Some clinical studies suggested that ecoimmunonutrition (EIN) as a new strategy had better treatment effect on SAP patients. But the experiment studies on the precise mechanism of the eff...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2006-04-20 00:00:00
abstract：OBJECTIVE:This article aims to present a brief profile of the advances in prevention and treatment of myocardial injury in cancer therapy based on relevant literature or reports. DATA SOURCES:The data cited in this review were obtained from articles indexed in PubMed and China National Knowledge Internet (CNKI) up to ...
journal_title：Chinese medical journal
pub_type： 杂志文章,评审
doi：10.1097/CM9.0000000000000498
更新日期：2019-11-20 00:00:00
abstract：BACKGROUND:Contrast induced acute kidney injury (CIAKI) is an important complication in the use of iodinated contrast media (CM). Our study was to evaluate the neutrophil gelatinase-associated lipocalin (NGAL) and cystatin C for early diagnosis of CIAKI. METHODS:The patients with established or suspected coronary arte...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2012-03-01 00:00:00
abstract：Background:Increasing evidence has supported the link of intestinal Fusobacterium nucleatum infection to colorectal cancer (CRC). However, the value of F. nucleatum as a biomarker in CRC detection has not been fully defined. In order to reduce the random error and bias of individual research, this meta-analysis aimed t...
journal_title：Chinese medical journal
pub_type： 杂志文章,meta分析
doi：10.4103/0366-6999.232814
更新日期：2018-06-05 00:00:00
abstract：BACKGROUND:Although a variety of risk factors of pneumonia after clipping or coiling of the aneurysm (post-operative pneumonia [POP]) in patients with aneurysmal subarachnoid hemorrhage (aSAH) have been studied, the predictive model of POP after aSAH has still not been well established. Thus, the aim of this study was ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.1097/CM9.0000000000001304
更新日期：2020-12-24 00:00:00
abstract：BACKGROUND:Rituximab is used extensively in combination with chemotherapy to cure non-Hodgkin's lymphoma (NHL), and not only accelerates short-term improvement, but also prolongs patient survival and decreases receptor relapse. The aim of this study was to evaluate the impact of Fcgamma IIIA (FcgammaRIIIA) gene polymor...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2010-01-20 00:00:00
abstract：:Carbamyl phosphate synthetase I (CPS1) is an initial enzyme of urea synthetase system. It exists exclusively in liver cells and epithelial cells of the small intestine. By immunocytochemistry, 70.5% of 88 surgically resected gastric carcinomas (42 advanced and 46 early gastric carcinomas) was found to be CPS1 immunore...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1989-08-01 00:00:00
abstract：BACKGROUND:The conventional method cannot guarantee the precise osteotomies required for a perfect realignment and a better prognosis after total knee arthroplasty (TKA). This study investigated a customized guide plate for osteotomy placement in TKAs with the aid of the statistical shape model technique using weight-b...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.4103/0366-6999.176082
更新日期：2016-02-20 00:00:00
abstract：BACKGROUND:Exposure of adult mice to more than 95% O(2) produces a lethal injury by 72 hours. Nitric oxide synthase (NOS) is thought to contribute to the pathophysiology of murine hyperoxia-induced acute lung injury (ALI). Osteopontin (OPN) is a phosphorylated glycoprotein produced principally by macrophages. OPN inhib...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2010-04-05 00:00:00
abstract：OBJECTIVE:To describe the methods which were used to develop collagen-based materials for wound dressing. METHODS:Fresh frozen bovine tendon was treated with 0.05 mol/L acetic acid at pH 3.2 for 48-72 hours, homogenized, filtered, mixed with 8% chondroitin sulphate, for creating a deaerated 1.5%-2.5% collagen solution...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2003-03-01 00:00:00
abstract：BACKGROUND:The methods for the treatment of postcatheterization femoral arteriovenous fistulas (AVF-s) - simple observation, ultrasound guided compression, covered stents implantation and coil embolization have poor outcome. Surgery is the standard method for treatment of femoral AVFs, but it is a traumatic operation. ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2007-06-05 00:00:00
abstract：BACKGROUND:Robotic assisted minimally invasive cardiac sugery is a new technique that uses small port sites and peripheral vessel cannulation for cardiopulmonary bypass (CPB) has been used. The right internal jugular vein (IJV) is commonly used for intraoperative venous access to the central circulation and identified ...
journal_title：Chinese medical journal
pub_type： 临床试验,杂志文章
doi：
更新日期：2013-07-01 00:00:00
abstract：BACKGROUND:Cubital tunnel syndrome is a well-recognized clinical condition and is the second most common peripheral compression neuropathy. This study was designed to investigate the causes of cubital tunnel syndrome by surgical means and to assess the clinical value of the neurophysiological diagnosis of cubital tunne...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2004-09-01 00:00:00
abstract：OBJECTIVE:To investigate whether coagulation factor VII (FVII) polymorphisms play a role in the pathogenesis of coronary artery disease (CAD) and/or myocardial infarction (MI) in a series of Hans. METHODS:The Arg(353)Gln and HVR4 polymorphisms of FVII gene were determined in 374 patients undergoing selective coronary ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2003-08-01 00:00:00
abstract：BACKGROUND:The aim of this study was to investigate the potential relationship between the dynamic expression of Toll-like receptor 2 and 4 (TLR2/4) in peripheral blood mononuclear cells as well as changes in serum concentration of inflammatory factors and acute lung injury (ALI) in patients after orthotopic liver tran...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2009-04-20 00:00:00
abstract：OBJECTIVE:To investigate the role of a potential diabetes-related mitochondrial region, which includes two previously reported mutations, 3243A-->G and 3316G-->A, in Chinese patients with adult-onset type 2 diabetes. METHODS:A total of 277 patients and 241 normal subjects were recruited for the study. Mitochondrial nt...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2002-05-01 00:00:00
abstract：BACKGROUND:The chronic obstructive pulmonary disease assessment test (CAT) is an easy to use health-related quality of life questionnaire, the modified Medical Research Council (mMRC) dyspnea scale is a classic dyspnea scale which is widely used, while the correlation between them is still not clear. This study investi...
journal_title：Chinese medical journal
pub_type： 杂志文章,多中心研究
doi：
更新日期：2013-01-01 00:00:00
