Effect of burn healing liquid on keratinocyte and fibroblast proliferation and on collagen lattice contraction.

Abstract:

OBJECTIVES:To investigate the effect of Burn Healing Liquid (BHL) on the proliferation of keratinocytes and fibroblasts and to explore the potential effect of BHL on fibroblast contraction. METHODS:Human keratinocytes and dermal fibroblasts were cultured in media containing serial dilutions of BHL followed by cell proliferation determination assessed with MTT (3-[4, 5-dimehtylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide) assay at different time intervals. The in vitro collagen lattice contraction model was utilized for determining the contractility of fibroblasts cultured in BHL containing medium. RESULTS:The 1:10(7) dilution of BHL enhanced the growth of both keratinocytes and fibroblasts whereas the 1:10 dilution increased the growth of keratinocytes only. Collagen lattice contraction was inhibited dose-dependently by BHL and such an inhibition could be reversed by switching BHL containing medium to normal medium containing 10% fetal calf serum. CONCLUSION:BHL enhances the growth of both keratinocytes and fibroblasts and reversibly inhibits the fibroblast contraction in collagen lattice.

journal_name

Chin Med J (Engl)

journal_title

Chinese medical journal

authors

Su Y,Chen B,Thang PT,Hughes MA,Xu X,Cherry GW

keywords:

subject

Has Abstract

pub_date

1999-08-01 00:00:00

pages

720-4

issue

8

eissn

0366-6999

issn

2542-5641

journal_volume

112

pub_type

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